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TRANSLUCENT GREEN, an ERF Family Transcription Factor, Controls Water Balance in Arabidopsis by Activating the Expression of Aquaporin Genes

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Molecular Plant 2014年第4期7卷 601-615页

作者： Danling Zhu Zhe Wu Guangyu Cao Jigang Li Jia Wei Tomohiko Tsuge Hongya Gua Takashi aoyama Li-Jia Qu State Key Laboratory of Protein and Plant Gene Research Peking-Tsinghua Center for Life Sciences College of Life Sciences Peking University Beijing 100871 China State Key Laboratory of Plant Physiology and Biochemistry College of Biological Sciences China Agricultural University Beijing 100193 China Institute for Chemical Research Kyoto University Gokasho Uji Kyoto 611-0011 Japan The National Plant Gene Research Center (Beijing) Beijing 100101 China

Water is the most abundant molecule in almost all living organisms. Aquaporins are channel proteins that play critical roles in controlling the water content of cells. Here, we report the identification of an AP2/EREBP family transcription factor in Arabidopsis thaliana, TRANSLUCENT GREEN （TG）, whose overexpression in transgenic plants gave enhanced drought tolerance and vitrified leaves. TG protein is localized in the nucleus, binds DRE and GCC elements in vitro, and acts as a transcriptional activator in yeast cells. Microarray analysis revealed a total of 330 genes regulated by TG, among which five genes encode aquaporins. A transient expression assay showed that TG directly binds to the promoters of three aquaporin genes, such as AtTIP1;1, AtTIP2;3, and AtPIP2;2, indicating that TG directly regulates the expression of these genes. Moreover, overexpression of AtTIP1;1 resulted in vitrified phenotypes in transgenic Arabidopsis plants, similar to those observed in TG overexpression lines. Water injection into wild-type leaves recapitulated the vitrified leaf phenotypes, which was reversed by cutting off the water supply from vascular bundles. Taken together, our data support that TG controls water balance in Arabidopsis through directly activating the expression of aquaporin genes.

关键词： AP2/EREBP TRANSLUCENT GREEN vitrified leaf transcriptional regulation aquaporin.

An Advanced Oscillation Mixing Method for Improving the Cooling Uniformity in Quenching

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材料热处理学报 2004年第5期25卷 475-479页

作者： Hiroyuki Hoshino Hiromitsu Murakami Hideo Yokota Satoshi Suda hideki Tsuge Koichi Terasaka Toshiaki Wakabayashi Nippon Oil Processing CO. LTD Niigata JapanOriental engineering CO. LTD Tokyo JapanNippon Oil Corp. Yokohama JapanNippon Oil Processing CO. LTD Niigata JapanKeio Univ. Yokohama Japan Keio Univ. Yokohama Japan Kagawa Univ. Takamatsu Japan

In quenching, the cooling uniformity is most important to diminish distortion occurring on work pieces. As a trial to accomplish uniform cooling, therefore, there exist various mixing methods of a quenchant and the quenchant circulation with an external pump has so far been the well accepted mixing method. However, this study proposes an advanced oscillation mixing method that can improve more the cooling uniformity in quenching. The proposed method includes a stirrer in oscillating motion, so that the simultaneous oscillating and mixing movements of the stirrer are considered to provide effectively the uniform cooling characteristics for the quenchant. In comparison with the case of the circulation pump mixing, the investigation using the oscillation mixing method has demonstrated the following two experimental facts: (1) the short vapor blanket stage caused by the quick breakage of the oil vapor blanket and (2) the reduced variation of the quenching distortion.

关键词：淬火剂冷却能力变形振动混合

Modeling three-dimensional forest structures to drive canopy radiative transfer simulations of bidirectional reflectance factor

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International Journal of Digital Earth 2018年第10期11卷 981-1000页

作者： Wei Yang hideki Kobayashi Xuehong Chen Kenlo Nishida Nasahara Rikie Suzuki Akihiko Kondoh Center for Environmental Remote Sensing Chiba UniversityChibaJapan Department of Environmental Geochemical Cycle Research Japan Agency for Marine-Earth Science and TechnologyYokohamaJapan State Key Laboratory of Earth Surface Processes and Resource Ecology Beijing Normal UniversityBeijingPeople’s Republic of China Faculty of Life and Environmental Sciences University of TsukubaTsukubaJapan

Three-dimensional(3-D)Monte Carlo-based radiative transfer(MCRT)models are usually used for benchmarking in intercomparisons of the canopy radiative transfer(RT)***,the 3-D MCRT models are rarely applied to develop remote sensing algorithms to estimate essential climate variables of forests,due mainly to the difficulties in obtaining realistic stand structures for different forest biomes over regional to global ***,some of important tree structure parameters such as canopy height and tree density distribution have been available *** enables to run the intermediate complexities of the 3-D MCRT *** consequently developed a statistical approach to generate forest structures with intermediate complexities depending on the inputs of canopy height and tree *** aims at facilitating applications of the 3-D MCRT models to develop remote sensing retrieval *** proposed approach was evaluated using field measurements of two boreal forest stands at Estonia and USA,*** demonstrated that the simulations of bidirectional reflectance factor(BRF)based on the measured forest structures agreed well with the BRF based on the generated structures from the proposed approach with the root mean square error(RMSE)and relative RMSE(rRMSE)ranging from 0.002 to 0.006 and from 0.7%to 19.8%,*** of the computed BRF with corresponding MODIS reflectance data yielded RMSE and rRMSE lower than 0.03 and 20%,*** the results from the current study are limited in two boreal forest stands,our approach has the potential to generate stand structures for different forest biomes.

关键词： Bidirectional reflectance factor remote sensing forest structure radiative transfer model

Mechanisms of Steatosis-Derived Hepatocarcinogenesis:Lessons from HCV Core Gene Transgenic Mice

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Engineering 2021年第12期7卷 1797-1805页

作者： Pan Diao Fangping Jia Xiaojing Wang Xiao Hu Takefumi Kimura Takero Nakajima Toshifumi aoyama Kyoji Moriya Kazuhiko Koike Naoki Tanaka Department of Metabolic Regulation Shinshu University School of MedicineMatsumoto 390-8621Japan State Key Laboratory for Diagnosis and Treatment of Infectious Diseases National Clinical Research Center for Infectious DiseasesCollaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesDepartment of Infectious DiseasesThe First Affiliated HospitalCollege of MedicineZhejiang UniversityHangzhou 310003China Department of Gastroenterology Lishui HospitalZhejiang University School of MedicineLishui 323000China Department of Pathophysiology Hebei Medical UniversityShijiazhuang 050017China Department of Gastroenterology Shinshu University School of MedicineMatsumoto 390-8621Japan Molecular Signaling Section Laboratory of Bioorganic ChemistryNational Institute of Diabetes and Digestive and Kidney DiseasesNational Institutes of HealthBethesdaMD 20892USA Department of Infection Control and Prevention The University of TokyoTokyo 113-0033Japan Department of Gastroenterology The University of TokyoTokyo 113-0033Japan International Relations Office Shinshu University School of MedicineMatsumoto 390-8621Japan Research Center for Social Systems Shinshu UniversityMatsumoto 390-8621Japan

Hepatitis C virus(HCV)is a major cause of chronic hepatitis,liver cirrhosis,and hepatocellular carcinoma(HCC)*** the structural proteins of HCV,the HCV core protein has the ability to regulate gene transcription,lipid metabolism,cell proliferation,apoptosis,and autophagy,all of which are closely related to the development of *** mice carrying the HCV core gene exhibited age-dependent insulin resistance,hepatic steatosis,and HCC that resembled the clinical characteristics of chronic hepatitis C *** dietary modifications,including calorie restriction and diets rich in saturated fatty acids,trans fatty acids,or cholesterol,were found to influence hepatic steatogenesis and tumorigenesis in HCV core gene transgenic *** strategies modulated hepatocellular stress and proliferation,in addition to hepatic fibrotic processes and the microenvironment,thereby corroborating a close interconnection between dietary habits and steatosis-related *** this review,we summarize the findings obtained from mouse models transgenic for the HCV genome,with a special focus on HCV core gene transgenic mice,and discuss the mechanisms of steatogenesis and hepatocarcinogenesis induced by the HCV core protein and the impact of dietary habits on steatosis-derived HCC development.

关键词： Steatosis Hepatocellular carcinoma Trans fatty acid Saturated fatty acid Dietary restriction HCV core protein

Suppression of fibrosis in human pterygium fibroblasts by butyrate and phenylbutyrate

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International Journal of Ophthalmology(English edition) 2017年第9期10卷 1337-1343页

作者： Yuka Koga Noriaki Maeshige Hiroto Tabuchi Mikiko Uemura Michiko aoyama-Ishikawa Makoto Miyoshi Chikako Katakami Makoto Usami Division of Nutrition and Metabolism Department ofBiophysicsKobe University Graduate School of Health SciencesTomogaoka 7-10-2Suma-kuKobeJapan Department of Rehabilitation Science Kobe University Graduate School of Health SciencesTomogaoka 7-10-2Suma-kuKobeJapan Department of Ophthalmology Saneikai Tsukazaki HospitalWaku 68-1Aboshi-kuHimejiJapan Department of Nutrition Kobe University HospitalKobe University School of MedicineKusunoki-cho 7-5-2Chuo-kuKobeJapan

AIM：To evaluate the antifibrogenic effects of butyrate or phenylbutyrate,a chemical derivative of butyrate,in human pterygium ***：Human pterygium fibroblasts obtained from patient pterygium tissue were treated with butyrate or phenylbutyrate for *** ofα-smooth muscle actin,collagen I,collagen III and matrix metalloproteinase-1m RNA was measured by quantitative real-time reverse transcription polymerase chain reaction,and acetylated histone was evaluated by Western ***：Butyrate inhibitedα-smooth muscle actin,type III collagen and matrix metalloproteinase-1 expressions,and phenylbutyrate inhibited types I and III collagen and matrix metalloproteinase-1 expressions without changing cell viability as well as both of these increased histone *** results suggested that butyrate and phenylbutyrate suppress fibrosis through a mechanism involving histone deacetylase ***：This indicates that butyrate or phenylbutyrate have antifibrogenic effects in human pterygium fibroblasts and could be novel types of prophylactic and/or therapeutic drugs for pterygium,especially phenylbutyrate,which does not have the unpleasant smell associated with butyrate.

关键词： butyrate phenylbutymte pterygium fibroblasts antifibrogenic effect

Evaluating Rock Mass Properties of Vipingo Coral Limestone Quarry Based on a Modified Geological Strength Index (GSI) and State of Karstification

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Open Journal of Geology 2022年第1期12卷 57-79页

作者： Joan Atieno Onyango Takashi Sasaoka hideki Shimada Akihiro Hamanaka Dyson Moses Dintwe Tumelo Earth Resources Engineering Department Kyushu University Fukuoka Japan Mining Materials and Petroleum Engineering Department Jomo Kenyatta University of Agriculture and Technology Nairobi Kenya

The process of evaluating rock mass strength requires that major structural features such as joints that influence rock strength are considered. In carbonate rock masses, however, the strength of the rock mass is largely dependent on intact rock strength and structural features play a secondary role. Laboratory experiments on porous rock have shown that intact rock strength reduces with increasing porosity, which has a direct effect on the rock mass strength. Rock porosity has however not been well accounted for in rock mass characterization methods currently in use. This research applies the modified GSI method for carbonate rock masses which is based on a combination of GSI and total porosity. The main aim is to quantify the GSI with respect to rock porosity which is a direct indicator of the state of karstification, as an inherent feature that affects rock mass strength. An empirical equation is proposed whereby the GSI as observed in the field is modified by a natural log of the value of porosity, giving rise to a modified GSI (GSIm). The GSIm together with laboratory properties of rock is used to determine the properties of Vipingo coral limestone from RocLab software. A deterministic parametric slope stability analysis is done using the finite element software Phase 2 with the rock mass properties as input parameters. The analysis results point to a direct dependence of the slope stability on slope angle, slope height and rock mass strength of the lithological unit. The graphs make a useful design guide for slopes engineered in this type of rock mass.

关键词： Carbonates Karstification Porosity Rock Mass Strength Slope Stability Weak Rock

Reciprocal costimulatory molecules control the activation of mucosal type 3 innate lymphoid cells during engagement with B cells

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Cellular & Molecular Immunology 2023年第7期20卷 808-819页

作者： Xinping Lv Shan Zhu Jing Wu Jinfeng Shi Qiuyu Wei Tete Li Ning Yang Chunyan Liu Lingli Qi Guoxia Zang Hang Cheng Zhiguang Yang Chengyan Jin Yusheng Wang Jiuwei Cui hideki Ueno Yong-Jun Liu Jingtao Chen Cancer Center First Hospital of Jilin UniversityChangchunJilin 130021China Laboratory for Tumor Immunology First Hospital of Jilin UniversityChangchunJilin 130061China Department of Otolaryngology Head and Neck Surgery First Hospital of Jilin UniversityChangchunJilin 130021China Department of Translational Medicine Changchun GeneScience Pharmaceuticals Co.Ltd.ChangchunJilin 130012China Department of Gynecology First Hospital of Jilin UniversityChangchunJilin 130021China Department of Pediatric Gastroenterology First Hospital of Jilin UniversityChangchunJilin 130021China Department of Pediatrics First Hospital of Jilin UniversityChangchunJilin 130021China Department of Thoracic Surgery First Hospital of Jilin UniversityChangchunJilin 130021China Department of Thoracic Surgery Second Hospital of Jilin UniversityChangchunJilin 130041China Department of Immunology Graduate School of MedicineKyoto UniversityKyotoJapan ASHBi Institute for the Advanced Study of Human Biology Kyoto UniversityKyotoJapan

Innate lymphoid cells(ILCs)are the counterpart of T helper cells in the innate immune system and share multiple phenotypes with T helper *** T-cell costimulator(ICOS)is recognized on T cells and participates in T-cell activation and T and B-cell engagement in lymphoid ***,the role of ICOS in ILC3s and ILC3-involved interactions with the immune microenvironment remains ***,we found that ICOS expression on human ILC3s was correlated with the activated state of *** costimulation enhanced the survival,proliferation,and capacity of ILC3s to produce cytokines(IL-22,IL-17A,IFN-γ,TNF,and GM-CSF).Via synergistic effects of ICOS and CD40 signaling,B cells promoted ILC3 functions,and ILC3-induced T-cellindependent B-cell IgA and IgM secretion primarily required CD40 ***,ICOS is essential for the nonredundant role of ILC3s and their interaction with adjacent B cells.

关键词： Innate lymphoid cell B cell Tonsil ICOS Costimulatory molecule

Examination of in vivo mutagenicity of sodium arsenite and dimethylarsinic acid in gpt delta rats

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Journal of Environmental Sciences 2016年第11期28卷 125-130页

作者： Masaki Fujioka Min Gi Satoko Kawachi Kumiko Tatsumi Naomi Ishii Kenichiro Doi Anna Kakehashi hideki Wanibuchi Department of Molecular Pathology Osaka City University Graduate School of Medicine

Arsenic is a well-known human bladder and liver carcinogen, but its exact mechanism of carcinogenicity is not fully understood. Dimethylarsinic acid（DMAV） is a major urinary metabolite of sodium arsenite（i As~Ⅲ） and induces urinary bladder cancers in rats. DMAVand i As~Ⅲare negative in in vitro mutagenicity tests. However, their in vivo mutagenicities have not been determined. The purpose of present study is to evaluate the in vivo mutagenicities of DMAVand i As~Ⅲin rat urinary bladder epithelium and liver using gpt delta F344 ***-week old male gpt delta F344 rats were randomized into 3 groups and administered 0,92 mg/L DMAV, or 87 mg/L i As~Ⅲ（each 50 mg/L As） for 13 weeks in the drinking water. In the mutation assay, point mutations are detected in the gpt gene by 6-thioguanine selection（gpt assay） and deletion mutations are identified in the red/gam genes by Spi-selection（Spi-assay）. Results of the gpt and Spi-assays showed that DMAVand i As~Ⅲhad no effects on the mutant frequencies or mutation spectrum in urinary bladder epithelium or liver. These findings indicate that DMAVand i As~Ⅲare not mutagenic in urinary bladder epithelium or liver in rats.

关键词： Dimethylarsinic acid Sodium arsenite In vivo mutagenicity Urinary bladder epithelium gpt delta rat

Effects of Heavy Metal Pollution of Apple Orchard Surface Soils Associated with Past Use of Metal-Based Pesticides on Soil Microbial Biomass and Microbial Communities

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Journal of Environmental Protection 2013年第4期4卷 27-36页

作者： Masakazu aoyama Ryo Tanaka Faculty of Agriculture and Life Science Hirosaki University Hirosaki Japan

Apple orchard surface soils in Japan are polluted with copper (Cu), lead (Pb), and arsenic (As) due to long-term use of metal-based pesticides. We investigated the effects of heavy metals accumulated in the surface soils in apple orchards on the microbial biomass and the microbial communities. Soil samples were taken from a chestnut orchard (unpolluted control) and five apple orchards with different degrees of heavy metal pollution. Total concentrations of Cu, Pb, and As in soil ranged from 29 to 931 mg/kg, 35 to 771 mg/kg, and 11 to 198 mg/kg, respectively. The amount of microbial biomass carbon expressed on a soil organic carbon basis decreased with increasing concentrations of heavy metals. Thus, the heavy metals that accumulated in apple orchard surface soils had adverse effects on the soil microbial biomass. The analysis of phospholipid fatty acid (PLFA) composition indicated that the microbial community structure had changed because of the pesticide-derived heavy metals in soil. The relative abundance of gram-positive bacterial marker PLFAs increased and that of fungal marker PLFA decreased with increasing concentrations of heavy metals in soil. Denaturing gradient gel electrophoreses targeting the 16S ribosomal RNA gene of bacteria and the 18S ribosomal RNA gene of fungi also showed shifts in the composition of bacterial and fungal communities induced by soil pollution with heavy metals. However, the diversity of microbial communities was not significantly affected by the heavy metal pollution. This was attributable to the adaptation of the microbial communities in apple orchard surface soils to heavy metals derived from previously used pesticides.

关键词： Apple Orchard Denaturing Gradient Gel Electrophoresis Heavy Metals Microbial Biomass Metal-Based Pesticides Soil Pollution Phospholipid Fatty Acid