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豫西黄土高原果园生草对土壤线虫群落及结构的影响

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草地学报 2024年第1期32卷 96-104页

作者：包涵吕丹丹施赟贺雨轩赵金梦许革学李晓敏王冬河南大学生命科学学院河南开封475004 河南省灵宝市特色农业发展中心河南三门峡472500

土壤线虫是土壤生物中最丰富的后生动物,从线虫群落结构角度评价果园不同覆盖作物的土壤质量可为果园管理提供依据。2022年在黄土高原东部果园开展实验,供试品种为‘富士’苹果(Malus domestica cv. Fuji),处理包括:农田、单播毛苕子(Vi... 详细信息

土壤线虫是土壤生物中最丰富的后生动物,从线虫群落结构角度评价果园不同覆盖作物的土壤质量可为果园管理提供依据。2022年在黄土高原东部果园开展实验,供试品种为‘富士’苹果(Malus domestica cv. Fuji),处理包括:农田、单播毛苕子(Vicia sativa L.)、单播黑麦草(Lolium perenne L.)。与农田对照相比,覆盖毛苕子线虫总丰度及食细菌线虫相对丰度分别显著提高了302.33%和38.41%,植物寄生线虫与杂食和捕食线虫的相对丰度分别降低了62.18%和86.12%。覆盖黑麦草较对照处理导致杂食和捕食线虫的相对丰度降低了55.59%。土壤微生物碳含量、变形菌门相对丰度、土壤pH值、豆科生物量比例是影响线虫群落结构的主要因子。果园行间覆盖毛苕子可优化线虫群落结构,有利于果树根系的生长,建议豫西黄土高原果园生草选择豆科物种毛苕子为益。

关键词：黄土高原苹果园土壤线虫覆盖作物土壤微生物

Differential Proteomic Analysis of Carbon Ion Radiation in Sheep Sperm

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Journal of Integrative Agriculture 2013年第9期12卷 1629-1637页

作者： he yu-xuan LI Hong-yan ZHANG Yong he Jian-hua ZHANG Hong ZHAO Xing-xu College of Veterinary Medicine Gansu Agricultural University Department of Medical Physics Institute of Modern PhysicsChinese Academy of Sciences Key Laboratory of heavy Ion Radiation Medicine Chinese Academy of Sciences Key Laboratory of heavy Ion Radiation Medicine of Gansu Province University of Chinese Academy of Sciences Lanzhou Agricultural Science and Technology Research Promotion Center

This study is first to investigate proteomic changes in sheep sperm induced by carbon ion radiation using two-dimensional electrophoresis （2-DE） analysis in the project of breeding a new variety of sheep. Differential expression proteins were detected using the PDQuest 8.0 software after staining with Coomassie blue. Valid spots were then analyzed through liquid chromatography tandem mass spectrometry （LC-MS/MS）. Among the 480 total protein spots displayed in 2-D gels, 6 specific protein spots were observed in sperm gels. A search against protein sequences in the National Center for Biotechnology Information databases （NCBI） indicated that differentially expressed proteins correspond to two proteins, identified to be enolase and transcription factor AP-2-alpha （TFAP-2c0. The two proteins were up-regulated in the irradiated sperm. To the best of our knowledge, this study is the first to identify proteomic changes induced by carbon ion radiation in sheep sperm. The analysis of differential expression protein may be useful in identifying new breeding markers in sheep reproduction and in clarifying the mechanisms involved in irradiation or space breeding.

关键词： sheep sperm protein two-dimensional polyacrylamide gel electrophoresis analysis proteome carbon ionradiation irradiation breeding

基于柔性电极和信号放大技术构建检测磷脂酶C的电化学传感器

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应用化学 2024年第7期41卷 987-997页

作者：王瑶瑶孙铭阳杨钻杜绍恺何禹璇孙越辽宁师范大学化学化工学院大连116029

磷脂酶C(PLC)是动植物中重要的脂质水解酶,在哺乳动物细胞的代谢、生长、炎症、分化、衰老和凋亡中起着重要作用,与许多癌症的发生、发展显著相关。蛋白质催化原子转移自由基聚合(PATRP)是高分子设计、制备的有效手段,可通过链式反应将... 详细信息

磷脂酶C(PLC)是动植物中重要的脂质水解酶,在哺乳动物细胞的代谢、生长、炎症、分化、衰老和凋亡中起着重要作用,与许多癌症的发生、发展显著相关。蛋白质催化原子转移自由基聚合(PATRP)是高分子设计、制备的有效手段,可通过链式反应将数百个电活性单体连接成一个高相对分子质量的聚合物链,从而使分子识别信号大大增强。首先,在柔性碳布(CC)电极表面修饰纳米金(AuNPs),之后通过3-巯基丙酸、碳二亚胺盐酸盐和N-羟基丁二酰亚胺,将PLC的特异性底物磷脂酰乙醇胺(PE)固定在电极上,经过PLC对PE的特异性水解产生磷酸基团,引入Zr^(4+),通过Zr^(4+)的配位作用将聚合引发剂α-溴苯乙酸修饰到电极表面,最后在蛋白质催化作用下进行2,2,6,6-四甲基哌啶氧化物(TEMPO)的PATRP聚合反应,在电极表面引入电活性聚合物分子链,构建信号放大型电化学传感器P(TEMPO)/PE/AuNPs/CC,用于磷脂酶C的高灵敏度检测。结果表明,该传感器检测PLC的线性范围为10~1×10^(5)mU/L,检出限为3.568 mU/L(S/N=3)。与其他检测PLC的方法相比,该传感器具有较宽的检测范围和较低的检出限,且在检测实际乳腺癌细胞中的PLC方面有巨大的实际应用潜力。

关键词：磷脂酶C 原子转移自由基聚合柔性电极电化学传感器

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Segregation of binary particles in gas-solid fluidized bed

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Frontiers of Chemical Science and Engineering 2024年第6期18卷 113-121页

作者： Yaxiong yu Feng Lu xuan he Fei Wei Chenxi Zhang Beijing Key Laboratory of Green Chemical Reaction Engineering and Technology Department of Chemical EngineeringTsinghua UniversityBeijing 100084China School of Chemical Engineering and Technology Xi’an Jiaotong UniversityXi’an 710049China Ordos Laboratory Ordos 017010China Institute for Carbon Neutrality Tsinghua UniversityBeijing 100084China

Particle segregation and mixing behavior play a crucial role in industrial *** study investigates the saturated jetsam fraction,which indicates the maximum capacity of flotsam to entrain jetsam,in an initially separated binary fluidized bed with particle size *** to the value of saturated jetsam fraction,three distinct regimes-segregation,mixing,and an intermediate regime-are ***,intriguing relationships between the saturated jetsam fraction and superficial gas velocity are observed,exhibiting monotonic trends in both the segregation and mixing regimes,while a unique volcano-shaped curve in the intermediate ***,a comprehensive entrainment model based on two-fluid model elucidates the observed phenomena,emphasizing the significance of mixing behavior in fluidized layer on the saturated jetsam *** work offers potential insights for evaluating segregation in industrial applications.

关键词： fluidized bed segregation binary bubbles entrainment

鼠李糖脂的分离鉴定及其在食品中的应用研究进展

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食品安全质量检测学报 2024年第13期15卷 169-176页

作者：赫宇轩敖成翔李雨羲赵阳美瑾卢航大连海洋大学食品科学与工程学院大连116023

鼠李糖脂(rhamnolipids,RLs)是一种天然的具有多种生物活性的脂类物质,由于其具有促进发酵起泡、抑制细菌生物膜形成和调节免疫等功能,可广泛应用于食品、医药和农业等领域。目前工业上已经采用微生物发酵法对RLs进行生产制备。RLs作为... 详细信息

鼠李糖脂(rhamnolipids,RLs)是一种天然的具有多种生物活性的脂类物质,由于其具有促进发酵起泡、抑制细菌生物膜形成和调节免疫等功能,可广泛应用于食品、医药和农业等领域。目前工业上已经采用微生物发酵法对RLs进行生产制备。RLs作为最有前途的商业化生物表面活性剂,高效、准确的鉴定和表征RLs对其生产、应用至关重要。本文介绍了RLs的生产方法 ,重点概述RLs的分离纯化工艺和鉴定技术,并总结了RLs在食品领域的应用现状,为今后RLs活性开发及应用提供参考,推动其在食品领域的工业化进程。

关键词：鼠李糖脂生产鼠李糖脂分离纯化鉴定

首次非诱发性痫性发作后再发风险预测研究进展

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实用医院临床杂志 2024年第2期21卷 163-166页

作者：郑佳玉于明星张娜娜陈春燕何雨萱喻良成都中医药大学医学与生命科学学院四川成都610075 四川省医学科学院·四川省人民医院神经内科四川成都610072

首次非诱发性痫性发作后再发风险的预测较困难,导致癫痫的早期诊断存在难度。本文就近年来相关文献进行综述发现脑电图异常如慢波增加、双侧不对称或正常睡眠波消失、癫痫样放电,神经影像学异常如白质软化、胶质增生、脑软化、任何灰质... 详细信息

首次非诱发性痫性发作后再发风险的预测较困难,导致癫痫的早期诊断存在难度。本文就近年来相关文献进行综述发现脑电图异常如慢波增加、双侧不对称或正常睡眠波消失、癫痫样放电,神经影像学异常如白质软化、胶质增生、脑软化、任何灰质病变、肿块病变、出血、血管病变、海马异常,睡眠时首次痫性发作,癫痫家族史等均可显著增加再发风险,同时发现一些可能的潜在生物标志物如血清催乳素、S100B、白细胞介素-6等对首次非诱发性痫性发作后再发风险的预测具有一定价值,对癫痫的早期诊断有一定帮助。

关键词：癫痫预测再发风险脑电图首次非诱发性痫性发作

Ionizing Radiation-Induced RPL23a Reduction Regulates Apoptosis via RPL11-MDM2-p53 Pathway in Mouse Spermatogonia

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Biomedical and Environmental Sciences 2021年第10期34卷 789-802页

作者： he yu xuan GUO Yi Xiao ZHANG Yong HU Jun Jie DONG Wei Tao DU Xiang Hong ZHAO Xing Xu College of Veterinary Medicine Gansu Agricultural UniversityLanzhou 730070GansuChina Department of Radiation Oncology Gansu Provincial HospitalLanzhou 730000GansuChina

Objective The expression patterns of ribosomal large subunit protein 23 a(RPL23 a)in mouse testes and GC-1 cells were analyzed to investigate the potential relationship between RPL23 a expression and spermatogonia apoptosis upon exposure to *** Male mice and GC-1 cells were irradiated with X-ray,terminal dUTP nick end-labelling(TUNEL)was performed to detect apoptotic spermatogonia in *** rate and cell cycle phase of GC-1 cells were analyzed with flow *** interactions were detected by Immunoprecipitation and protein localization as studied by *** and real-time PCR were applied to analyze to protein and gene *** Ionizing radiation(IR)increased spermatogonia apoptosis,the expression of RPL11,MDM2 and p53,and decreased RPL23 a expression in mice spermatogonia in vivo and in ***23 a knockdown weakened the interaction between RPL23 a and RPL11,leading to p53 ***,knockdown and IR decreased RPL23 a that induces spermatogonia apoptosis via RPL23 a-RPL11-MDM2-p53 pathway in GC-1 *** These results suggested that IR reduced RPL23 a expression,leading to weakened the RPL23 a-RPL11 interactions,which may have activated p53,resulting in spermatogonia *** results provide insights into environmental and clinical risks of radiotherapy following exposure to IR in male *** graphical abstract was available in the web of ***.

关键词： Ionizing radiation Testis Spermatogonia apoptosis

Metal-to-insulator transition in platinum group compounds

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Rare Metals 2024年第8期43卷 3460-3474页

作者： yu-xuan Xia Jian-Gang he Nuo-Fu Chen Ji-Kun Chen School of New Energy North China Electric Power UniversityBeijing102206China School of Mathematics and Physics University of Science and Technology BeijingBeijing 100083China Key Laboratory of Advanced Materials and Devices for PostMoore Chips(Ministry of Education) School of Materials Science and EngineeringJniversity of Science and Technology BeijingBeijing 100083China

The metal-to-insulator transition(MIT) as usually achieved in 3d-orbital transitional metal(TM) compounds opens up a new paradigm in correlated electronics via triggering abrupt variations in their transportation *** to such 3d-orbital TM compounds,the MIT within the platinum group(Pg) element compounds based on the 4d-and 5d-orbital configurations is more complicated,owing to their elevation in the spinorbit coupling and meanwhile weakened intra-atomic Coulomb *** brings in a new freedom to regulate the balance in their metallic or semiconductive orbital configurations,while their MIT properties can be potentially combined with their spintronic properties to enable new electronic ***,we review the electronic transport and MIT behaviors within the existing family of Pg-containing compounds,particularly those showing first-order MIT behaviors that can be useful in correlated *** is also hoped that summarizing the presently reported Pg-containing MIT compounds will lead to the discovery of more new material families and/or new mechanisms associated with the Pg-containing compounds showing MIT properties.

关键词： Metal-to-insulator transition Platinum group compounds Electron correlation Spin-orbital coupling

Carbon Ion Irradiation Induces Reduction of β-tubulin in Sperm of Pubertal Mice

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Biomedical and Environmental Sciences 2014年第2期27卷 130-133页

作者： LI Hong Yan he yu xuan ZHANG Hong LIU yuan yuan MIAO Guo Ying ZHAO Qiu yue Department of heavy Ion Radiation Medicine Institute of Modern Physics Chinese Academy of Sciences Key Laboratory of heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences Key Laboratory of heavy Ion Radiation Medicine of Gansu Province University of Chinese Academy of Sciences College of Veterinary Medicine Gansu Agricultural University Department of radiotherapy Gansu Provincial Hospital

Microtubules are involved in a variety of cellular functions such as cell division, intracellular transport, maintenance of cell polarity and flagella and ciliary motility. The heterogeneity of tubulin and microtubule-associated proteins is responsible for these different microtubule functions. Many studies have confirmed that the structure and function of the different α-tubulin and β-tubulin subunits can affect the microtubule. The sperm axoneme microtubule has linear fiber filaments which are polymerized by heterodimeric a and β-tubulin, each with a molecular mass of approximately 50 kD[1].

关键词： head tubulin in Sperm of Pubertal Mice Carbon Ion Irradiation Induces Reduction of