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Quantitative trait locus analysis of lateral branch-related traits in cucumber(Cucumis sativus L.)using recombinant inbred lines

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Science China(Life Sciences) 2008年第9期51卷 833-841页

作者： JIANG Su YUAN XiaoJun PAN JunSong he huanle CAI Run School of Agriculture and Biology Shanghai Jiaotong UniversityShanghai 200240China

A group of 224 recombinant inbred lines (RILs) was derived from a narrow cross between 2 cucumber (Cucumis sativus L.) lines, namely, S94 (Northern China type with weak lateral branch growth potential and early lateral branch sprouting time) and S06 (Northern European type with strong lateral branch growth potential and late lateral branch sprouting time). These lines were then used for investigating lateral branch-related traits. A total of 36 quantitative trait loci (QTLs) were detected for the following 4 lateral branch-related traits: lateral branch average length (LBAL), lateral branch total length (LBTL), lateral branch number (LBN), and first lateral branch node (FLBN). Further, each QTL explained 3.1% (lbtl2.1, spring) to 32.3% (lbn2.3, spring) of the observed phenotypic variance. Eleven QTLs (lbal1.1, lbtl1.1, lbn1.2, flbn1.2, etc.) for different traits were found to be clustered on the e23m18d-ME23EM6c section (7.4 cM) of linkage group (LG) 1; further, 15 QTLs (lbal2.1, lbtl2.1, lbn2.1, flbn2.1, etc.) were found to be clustered on the S94A1-ME4SA4a section (13.9 cM) of LG2. Twenty-one QTLs explained more than 10% of the phenotypic variance. Moreover, lbtl1.3 (autumn, 26.2%, logarithm of odds (LOD) = 17.4; spring, 26.9%, LOD = 17.9) had stable position and contribution in both seasons. Several se-quence-anchor markers (CMBR40, F, CS30, S94A1, CSWTA11B, etc.) were closely linked with some QTLs for LBAL, LBTL, LBN, and FLBN, which can be used for the marker-assisted selection to improve the plant architecture in cucumber breeding.

关键词： Cucumis sativus L. lateral branching recombinant inbred lines quantitative trait loci

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Construction of a cucumber genetic linkage map with SRAP markers and location of the genes for lateral branch traits

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Science China(Life Sciences) 2005年第3期48卷 213-220页

作者： WANG Gang PAN Junsong LI Xiaozun he huanle WU Aizhong CAI Run Agriculture and Biology School of Shanghai Jiaotong University Shanghai201101China

Using SRAP(sequence-related amplified polymorphism)markers a genetic linkage map of cucumber was constructed with a population consisting of 138 F_(2) individuals derived from a cross of the two cucumber lines,S06 and *** the survey of parental polymorphisms with 182 primer combinations,64 polymorphism-revealing primer pairs were screened out,which generated totally 108 polymorphic bands with an average of 1.7 bands per primer pair and at most 6 bands from one primer *** constructed molecular linkage map included 92 loci,distributed in seven linkage groups and spanning 1164.2 cM in length with an average genetic distance of 12.6 cM between two neighboring *** on this linkage map,the quantitative trait loci(QTL)for the lateral branch number(lbn)and the lateral branch average length(lbl)in cucumber were identified by QTLMapper1.6.A major QTL lbn1 located between ME11SA4B and ME5EM5 in LG2 could explain 10.63%of the total variation with its positively effecting allele from S06.A major QTL lbl1 located between DC1OD3 and DC1EM14 in LG2 could account for 10.38%of the total variation with its positively effecting allele from S_(06).

关键词： cucumber SRAP(sequence-related amplified polymorphism) molecular linkage map lateral branch gene quan-titative trait loci(QTL).

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Efficient Transposition of the Retrotransposon Tnt1 in Cucumber(Cucumis sativus L.)

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Horticultural Plant Journal 2018年第3期4卷 111-116页

作者： ZHANG Qi DU Hui LV Duo XIAO Tingting PAN Jian he huanle WANG Gang CAI Run WENG Yiqun PAN Junsong Plant Science Department School of Agriculture and BiologyShanghai Jiao Tong University USDA-ARS Vegetable crops Research UnitHorticulture DepartmentUniversity of Wisconsin

Tnt1 is an active retrotransposon originally identified in tobacco(Nicotiana tabacum L.)(Grandbastien et al.,1989),but its transposition activity could be activated through tissue culture in other plant *** insertions are stable and inheritable in the progeny,which has made it a valuable and versatile tool for developing insertional mutagenesis libraries in several plant ***,we explored its utility for mutagenesis in cucumber(Cucumis sativus L.).T_3 Tnt1 transgenic cucumber plants were subjected to tissue culture to regenerate self-pollinated *** PCR and analyses and Southern hybridization,we found regenerated plants maintained the original Tnt1 insertion and created new insertions suggesting characteristic re-transposition activity of Tnt1 during this *** genome walking,some flanking sequences of Tnt1 insertions were recovered in regenerated *** results demonstrated that Tnt1 could be stably inherited and re-transposable during tissue culture in cucumber and that it is feasible to use for developing an insertional mutagenesis library for cucumber.

关键词： cucumber Tnt1 retrotransposon tissue culture mutagenesis library

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Construction of a genetic map with SRAP markers and localization of the gene responsible for the first-flower-node trait in cucumber (Cucumis sativus L.)

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Progress in Natural Science:Materials International 2005年第5期15卷 407-413页

作者： PAN Junsong*, WANG Gang*, LI Xiaozun, he huanle, WU Aizhong and CAI Run**(Agriculture and Biology School, Shanghai Jiaotong University, Shanghai 201101, China) Agric. and Biology Sch. Shanghai Jiaotong Univ. Shanghai 201101 China

With an F 2 population from the cross of two cucumber inbred lines, S06 and S52, sequence-related amplified polymorphism (SRAP) was used to construct a genetic linkage map in cucumber (Cucumis sativus L.). Sixty-four SRAP primer combinations generated 108 polymorphic bands in the F 2 population analysis. The average of polymorphic bands produced by one primer pair was 1.5, and the maximum was 5. Using Mapmaker 3.0, a linkage map was constructed, which consisted of 77 SRAP markers distributed in nine linkage groups (LOD≥3.0) and spanned 1114.2 cM with an average interval of 14.5 cM between markers. The gene for the first-flower-node trait, termed ffn, was mapped to linkage group Ⅸ, flanked by DC1EM5 and ME7EM2A at 10.3 cM and 12.1 cM distance, respectively.

关键词： cucumber (Cucumis sativus L.) SRAP (sequence-related amplified polymorphism) linkage map first flower node.

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Construction of a BAC library from cucumber (Cucumis sativus L.) and identification of linkage group specific clones

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Progress in Natural Science:Materials International 2008年第2期18卷 143-147页

作者： Yuan Guan a,1,Qi Chen b,1,Junsong Pan a,Zheng Li a,huanle he a,Aizhong Wu a,Rentao Song b,Run Cai a,a School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240,China b School of Life Sciences,Shanghai University,Shanghai 200444,China School of Agriculture and Biology Shanghai Jiao Tong University Shanghai 200240 China School of Life Sciences Shanghai University Shanghai 200444 China

A bacterial artificial chromosome (BAC) library consisting of 19,200 clones with an average insert size of 105 kb has been constructed from a cucumber (Cucumis sativus L.) inbred line S94,derived from a cultivar in North China. The entire library was equivalent to approximately 5 haploid cucumber genomes. To facilitate chromosome engineering and anchor the cucumber genetic linkage map to its chromosomes,15 sequence-characterized ampli?ed regions (SCAR) and seven simple sequence repeats (SSR) markers from each link-age group of cucumber were used to screen an ordered array of pooled BAC DNA with polymerase chain reaction (PCR). Fifteen mark-ers gave at least two positive clones. As a result,22 BAC clones representing 7 linkage groups of cucumber were identified,which further validated the genome coverage and utility of the library. This BAC library and linkage group specific clones provide essential resources for future research of the cucumber genome.

关键词： Cucumber BAC library Molecular markers PCR screening Specific clones

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Advances in ice avalanches on the Tibetan Plateau

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Journal of Mountain Science 2024年第6期21卷 1814-1829页

作者： TANG Minggao LI Guang ZHAO huanle XU Qiang WU Guangjian YANG Wei GUO Daojing State Key Laboratory of Geohazard Prevention and Geoenvironment Protection Chengdu University of TechnologyChengdu 610059China College of Environment and Civil Engineering Chengdu University of TechnologyChengdu 610059China Institute of Tibetan Plateau Research Chinese Academy of SciencesBeijing 100101China

As some of the greatest natural disasters in the cryosphere,ice avalanches(IAs)seriously threaten lives and cause catastrophic damage to the resource environment,but a comprehensive overview of the state of knowledge on IAs remains *** summarized 63 IAs on the Tibetan Plateau(TP)since the 20th century,of which,over 20 IAs occurred after the 21st *** distributions of IAs are mainly concentrated in the southeastern and northwestern TP,and the occurrence time of IAs is mostly concentrated from July to *** highlight recent advances in mechanical properties and genetic mechanisms of IAs and emphasize that temperature,rainfall,and seismicity are the inducing *** failure modes of IAs are summarized into 6 categories by examples:slip pulling type,slip toppling type,slip breaking type,water level collapse type,cave roof collapse type,and wedge failure ***,we deliver recommendations concerning the risk assessment and prediction of *** results provide important scientific value for addressing climate change and resisting glacier-related hazards.

关键词： Ice avalanche Global warming Genetic mechanism Risk assessment Tibetan Plateau

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QTL molecular marker location of powdery mildew resistance in cucumber (Cucumis sativus L.)

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Science China(Life Sciences) 2008年第11期51卷 1003-1008页

作者： LIU LongZhou1,2, CAI Run1, YUAN XiaoJun1, he huanle1 & PAN JunSong1 1 School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China 2 Horticultural Research Institute, Shanghai Academy of Agricultural Sciences( (SAAS)), Shanghai Key Lab of Protected Horti-cultural Technology, Shanghai, 201106, China School of Agriculture and Biology Shanghai Jiaotong University Shanghai China Horticultural Research Institute Shanghai Academy of Agricultural Sciences (SAAS) Shanghai Key Lab of Protected Horticultural Technology Shanghai China

The cucumber lines, S94 (Northern China open-field type, powdery mildew (PM) susceptible) and S06 (European greenhouse type, PM resistant), and their F6:7 populations were used to investigate PM re-sistance under seedling spray inoculation in 2005/Autumn and 2006/Spring. QTL analysis was under-taken based on a constructed molecular linkage map of the corresponding F6 population using com-posite interval mapping. A total of four QTLs (pm1.1, pm2.1, pm4.1 and pm6.1) for PM resistance were identified and located on LG 1, 2, 4 and 6, respectively, explaining 5.2%-21.0% of the phenotypic variation. Three consistent QTLs (pm1.1, pm2.1 and pm4.1) were detected under the two test conditions. The QTL pm6.1 was only identified in 2005/Autumn. The total phenotypic variation explained by the QTLs was 52.0% and 42.0% in 2005/Autumn and 2006/Spring, respectively. Anchor markers tightly linked to those loci (he PM-resistance gene in cucumber.

关键词： cucumber powdery mildew QTL F6:7families seedling

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单性结实黄瓜主要农艺性状的遗传相关和通径分析

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北方园艺 2005年第6期 54-55页

作者：何欢乐蔡润潘俊松上海交通大学农业与生物学院上海201101

利用全雌性单性结实黄瓜(Cucumis sativusL.)的34个杂交组合及1个对照品种碧玉,对黄瓜主要性状进行遗传相关和通径分析。相关与通径分析表明供试35个黄瓜材料的9个农艺性状对产量构成的影响不同。其中,单果重和瓜条数对产量影响最大,相... 详细信息

利用全雌性单性结实黄瓜(Cucumis sativusL.)的34个杂交组合及1个对照品种碧玉,对黄瓜主要性状进行遗传相关和通径分析。相关与通径分析表明供试35个黄瓜材料的9个农艺性状对产量构成的影响不同。其中,单果重和瓜条数对产量影响最大,相关系数分别为0.516和0.767,达极显著水平;直接通径系数分别为0.687和0.631,单果重和瓜条数对产量有直接的影响作用。叶面积、株高、单果长度和单果直径等性状和产量的相关系数分别为0.523、0.652、532和668也为极显著水平,但它们对产量的直接通径系数仅为0.059、0.086、0.078和0.064,对产量的直接影响不大,而从间接通径系数上看,它们通过单果重的通径系数比较大,因此,这四个性状对产量的影响是通过影响单果重而达到的。

关键词：黄瓜全雌性单性结实农艺性状遗传相关通径分析

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Honeycomb-like biochar framework coupled with Fe_(3)O_(4)/FeS nanoparticles as efficient heterogeneous Fenton catalyst for phenol degradation

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Journal of Environmental Sciences 2024年第2期136卷 390-399页

作者： Aihua Cheng Yi he Xiaohe Liu Chi he Department of Environmental Engineering College of Geology and EnvironmentXi’an University of Science and TechnologyXi’an 710054China State Key Laboratory of Multiphase Flow in Power Engineering School of Energy and Power EngineeringXi’an Jiaotong UniversityXi’an 710049China National Engineering Laboratory for VOCs Pollution Control Material and Technology University of Chinese Academy of SciencesBeijing 101408China

Achieving an efficient and stable heterogeneous Fenton reaction over a wide pH range is of great significance for wastewater ***,a pollen-derived biochar catalyst with a unique honeycomb-like structure,coupled with the dispersion of magnetic Fe_(3)O_(4)/FeS(Fe/S)nanoparticles,was synthesized by simple impregnation precursor,followed by *** prepared Fe/S-biochar catalyst demonstrated outstanding phenol degradation efficiency across a wide pH range,with 98%of which eliminated even under neutral conditions(pH 7.0).The high catalytic activity was due to the multilevel porous structure of pollenderived biochar provided enough active sites and allowed for better electron transfer,then increases oxidation ability to promote the ***,the acid microenvironment formed by SO_(4)^(2-)group from Fe/S composite extended the pH range for Fenton reaction,and S^(2-)facilitated the conversion of≡Fe^(3+)to≡Fe^(2+),resulting in remarkable degradation ***,biochar can effectively promote cycling stability by limiting Fe *** work may provide a general strategy for designing 3D framework biochar-based Fe/S catalysts with excellent performance for heterogeneous Fenton reactions.

关键词： Biomass Biochar Fenton reaction Fe_(3)O_(4)/FeS(Fe/S) Phenol degradation

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北欧温室黄瓜申绿72的选育

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长江蔬菜 2005年第5期 36-37页

作者：何欢乐蔡润潘俊松上海交通大学农业与生物学院 201101

北欧温室型黄瓜申绿72是温室生产专用的一代杂种,其两个亲本分别来源于以色列和荷兰,经过7～8代的自交纯化后,配制成杂交组合申绿72。适应温室栽培,耐弱光性强,抗性好,产量高,适合在保护地推广。

关键词：温室黄瓜北欧选育一代杂种温室生产杂交组合温室栽培耐弱光性温室型以色列产量高保护地亲本自交抗性

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