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An iterative Rankine BEM for wave-making analysis of submerged and surface-piercing bodies in finite water depth

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Journal of Hydrodynamics 2013年第6期25卷 839-847页

作者： he guanghua Department of Naval Architecture and Ocean Engineering Osaka University

A 3-D iterative Rankine Boundary Element Method （BEM） for seakeeping problem in time domain is developed in the framework of linear potential theory. Waves generated by both submerged and surface-piercing bodies moving at a constant forward speed in otherwise calm water, and the resultant steady wave pattern, wave profile and resistance are computed to validate this newly-developed code. A rectangular computational domain moving with the same forward speed as the body is introduced, in which an artificial damping beach is installed at an outer portion of the free surface except the downstream side for satisfying the radiation condition. The velocity potential on the ship hull and the normal velocity on the free surface are obtained directly by solving the boundary integral equation, with the Rankine source used as the kernel function. An iterative time-marching scheme is employed for updating both kinematic and dynamic free surface boundary conditions to stabilize the calculation. Extensive results including the wave patterns, wave profiles and wave resistances for a submerged spheroid and a Wigley hull with forward speed are presented to validate the efficiency of the proposed 3-D time-domain higher-order approach. Finally, the sensitivity of ship-generated waves to the water depth is investigated. Computed results show satisfactory agreement with the corresponding experimental data and other numerical solutions.

关键词： wave resistance wave pattern time-domain Boundary Element Method (BEM) iterative method

The auxin transporter OsAUX1 regulates tillering in rice(Oryza sativa)

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Journal of Integrative Agriculture 2024年第5期23卷 1454-1467页

作者： Luqi Jia Yongdong Dai Ziwei Peng Zhibo Cui Xuefei Zhang Yangyang Li Weijiang Tian guanghua he Yun Li Xianchun Sang Chongqing Key Laboratory of Crop Molecular Improvement/Rice Research Institute Academy of Agricultural SciencesSouthwest UniversityChongqing400715China Rice and Sorghum Research Institute Sichuan Academy of Agricultural Sciences/Key Laboratory of Southwest Rice Biology and Genetic BreedingMinistry of Agriculture and Rural AffairsDeyang 618000China

Tillering is an important agronomic trait of rice(Oryza sativa)that affects the number of effective panicles,thereby affecting *** phytohormone auxin plays a key role in *** we identified the high tillering and semi-dwarf 1(htsd1)mutant with auxin-deficiency root characteristics,such as shortened lateral roots,reduced lateral root density,and enlarged root ***1 showed reduced sensitivity to auxin,but the external application of indole-3-acetic acid(IAA)inhibited its *** identified the mutated gene in htsd1 as AUXIN1(OsAUX1,LOC_Os01g63770),which encodes an auxin influx *** promoter sequence of OsAUX1 contains many SQUAMOSA PROMOTER BINDING PROTEIN-LIKE(SPL)binding sites,and we demonstrated that SPL7 binds to the OsAUX1 *** BRANCheD1(OsTB1),a key gene that negatively regulates tillering,was significantly downregulated in *** was enhanced in the OsTB1 knockout mutant,and the external application of IAA inhibited tiller elongation in this *** OsTB1 restored the multi-tiller phenotype of *** results suggest that SPL7 directly binds to the OsAUX1 promoter and regulates tillering in rice by altering OsTB1 expression to modulate auxin signaling.

关键词： rice(Oryza sativa L.) tillering indole-3-acetic acid(IAA) OsAUX1 OsTB1

A polygalacturonase gene OsPG1 modulates water homeostasis in rice

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The Crop Journal 2024年第1期12卷 79-91页

作者： Qinwen Zou Ranran Tu Jiajun Wu Tingting Huang Zhihao Sun Zheyan Ruan Hongyu Cao Shihui Yang Xihong Shen guanghua he Hong Wang Rice Research Institute Key Laboratory of Application and Safety Control of Genetically Modified CropsAcademy of Agricultural SciencesSouthwest UniversityChongqing 400715China State Key Laboratory of Rice Biology and Breeding Key Laboratory for Zhejiang Super Rice ResearchChina National Center for Rice ImprovementChina National Rice Research InstituteHangzhou 311401ZhejiangChina

A dynamic plant architecture is the basis of plant adaptation to changing *** many genes regulating leaf rolling have been identified,genes directly associated with water homeostasis are largely ***,we isolated a rice mutant,dynamic leaf rolling 1(dlr1),characterized by‘leaf unfolding in the morning-leaf rolling at noon-leaf unfolding in the evening’during a sunny *** content was decreased in rolled leaves and water sprayed on leaves caused reopening,indicating that in vivo water deficiency induced the leaf ***-based cloning and expression tests demonstrated that an A1400G single base mutation in Oryza sativa Polygalacturonase 1(OsPG1)/PHOTO-SENSITIVE LEAF ROLLING 1(PSL1)was responsible for the dynamic leaf rolling phenotype in the dlr1 ***1 encodes a polygalacturonase,one of the main enzymes that degrade demethylesterified homogalacturonans in plant cell ***1 was constitutively expressed in various tissues and was enriched in *** of the OsPG1 gene exhibited defects in stomatal closure and decreased stomatal density,leading to reduced transpiration and excessive water loss under specific conditions,but had normal root *** analysis revealed that mutation of OsPG1 led to reduced pectinase activity in the leaves and increased demethylesterified homogalacturonans in guard *** findings reveal a mechanism by which OsPG1 modulates water homeostasis to control dynamic leaf rolling,providing insights for plants to adapt to environmental variation.

关键词： Photosynthesis Stomata Transpiration Leaf rolling

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基于深度学习的海工平台运动预测

哈尔滨工业大学学报 2024年第8期56卷 163-170页

作者：薛佳帆张航维何广华姜泽成哈尔滨工业大学(威海)海洋工程学院山东威海264209 哈尔滨工业大学机电工程学院哈尔滨150001 山东船舶技术研究院山东威海264209

为提高海上作业装备的安全性能,实现海上结构物运动的实时预报,本研究利用了一种卷积神经网络(convolutional neural network, CNN)方法与长短期记忆(long short-term memory, LSTM)方法混合的深度学习模型用于运动预测,该混合模型运用... 详细信息

为提高海上作业装备的安全性能,实现海上结构物运动的实时预报,本研究利用了一种卷积神经网络(convolutional neural network, CNN)方法与长短期记忆(long short-term memory, LSTM)方法混合的深度学习模型用于运动预测,该混合模型运用CNN提取运动数据的特征,采用LSTM学习提取出的特征间的时间关系,并结合贝叶斯(Bayesian)优化算法优化混合模型的超参数。首先,对海工平台进行数值仿真,将得到的纵荡运动数据作为实验数据。其次,将数据集划分为训练集、验证集与测试集,利用训练集与验证集进行训练与验证,获得6、12、18 s运动最优预测模型,并利用测试集与LSTM模型进行比较。结果表明:混合模型在6、12、18 s预测上相比于LSTM等模型,预测精度可以提高15%~30%。除此之外,本研究还分别探究了预测精度与输入时长、预测时长之间的关系,结果显示预测精度受输入时长的影响较小,但随着预测时长的增加近似呈线性下降趋势。最后结合训练耗时证明了混合模型相比于LSTM等模型更具有优势。

关键词：贝叶斯优化自动机器学习运动预测 CNN LSTM

SUPER WOMAN 2(SPW2)maintains organ identity in spikelets by inhibiting the expression of floral homeotic genes OsMADS3,OsMADS58,OsMADS13,and DROOPING LEAF

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Journal of Integrative Agriculture 2024年第1期23卷 59-76页

作者： Hui Zhuang Jinsong Lan Qiuni Yang Xiaoyu Zhao Yuhuan Li Jingya Zhi Yalin Shen guanghuahe Yunfeng Li Rice Research Institute Key Laboratory of Application and Safety Control of Genetically Modified CropsAcademy of Agricultural SciencesSouthwest UniversityChongqing 400715China

Flower organ identity in rice is mainly determined by the A-,B-,C-and E-class genes,with the majority encoding MADS-box transcription ***,few studies have investigated how the expression of these floral organ identity genes is regulated during flower *** this study,we identified a gene named SUPER WOMAN 2(SPW2),which is necessary for spikelet/floret development in rice by participating in the regulation of the expression of pistil identity genes such as OsMADS3,OsMADS13,OsMADS58 and *** the spw2 mutant,ectopic stigma/ovary-like tissues were observed in the non-pistil organs,including sterile lemma,lemma,palea,lodicule,and stamen,suggesting that the identities of these organs were severely affected by mutations in ***2 was shown to encode a plant-specific EMF1-like protein that is involved in H3K27me3 modification as an important component of the PRC2 *** analysis showed that the SPW2 mutation led to the ectopic expression of OsMADS3,OsMADS13,OsMADS58,and DL in non-pistil organs of the *** ChIP-qPCR results showed significant reductions in the levels of H3K27me3 modification on the chromatin of these ***,we demonstrated that SPW2 can mediate the process of H3K27me3 modification of pistil-related genes to regulate their expression in non-pistil organs of spikelets in *** results of this study expand our understanding of the molecular mechanism by which SPW2 regulates floral organ identity genes through epigenetic regulation.

关键词： rice(Oryza sativa) spikelet organ identity H3K27me3

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基于尺度分割和特征优选的外破检测技术

航天返回与遥感 2024年第4期45卷 150-162页

作者：何光华黄薛凌张志坚国网江苏省电力有限公司无锡供电公司无锡214000

高分辨率遥感卫星是识别外力破坏的巡查中不可或缺的有效工具,而实现外力破坏迹象识别的关键在于变化检测技术的应用。文章提出一种基于最优尺度分割和特征优选的对象级变化检测方法,首先通过尺度评估参数确定最优分割尺度;然后基于最... 详细信息

高分辨率遥感卫星是识别外力破坏的巡查中不可或缺的有效工具,而实现外力破坏迹象识别的关键在于变化检测技术的应用。文章提出一种基于最优尺度分割和特征优选的对象级变化检测方法,首先通过尺度评估参数确定最优分割尺度;然后基于最优分割尺度结果,分别采用J-M距离算法和XGBoost模型对多源特征集进行优选,对比分析筛选出合适的特征集合;最后采用支持向量机(SVM)分类器计算各特征参数间的欧式距离并进行二分类,得到变化和未变化两种分类结果。结果表明,XGBoost模型算法进行特征优选后的精度指标均高于J-M距离算法的变化检测结果,并将同一分割尺度和特征集合应用于不同分辨率影像中验证变化检测结果,在面积和对象两种精度评估中,F1分数分别达到了83.31和84.09,而查准率均超过了79%,查全率也均超过了86%,说明文章使用的算法在不同分辨率下能获得较高的检测精度,同时减少了人工对特征选择和特征集建立规则的干预,为电缆沿线变化检测提供有效的技术支撑。

关键词：面向对象尺度分割特征优化变化检测遥感应用

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适冷β-半乳糖苷酶及其在食品工业中的应用

食品与发酵工业 2024年第3期50卷 314-320页

作者：周玲龚金炎何光华肖功年许韩山李玲浙江科技学院生物与化学工程学院浙江杭州310023 杭州市临平区质量计量监测中心浙江杭州311199

β-半乳糖苷酶,又称乳糖酶,广泛存在于细菌、真菌、酵母菌等微生物中,主要功能是乳糖水解和合成低聚半乳糖。利用β-半乳糖苷酶生产低乳糖牛奶等乳制品成为解决乳糖不耐受问题最有效的途径,然而常用的商业化β-半乳糖苷酶的最适反应温... 详细信息

β-半乳糖苷酶,又称乳糖酶,广泛存在于细菌、真菌、酵母菌等微生物中,主要功能是乳糖水解和合成低聚半乳糖。利用β-半乳糖苷酶生产低乳糖牛奶等乳制品成为解决乳糖不耐受问题最有效的途径,然而常用的商业化β-半乳糖苷酶的最适反应温度大多较高,对pH的要求比较严格,存在生产成本较高、消耗能量高等问题。适冷β-半乳糖苷酶在低温下也具有较高的酶活性,广泛应用于食品行业中,尤其在乳品工业。在低温下水解乳糖,生产低乳糖或无乳糖乳制品,供乳糖不耐受者食用,可降低成本、节约能源,具有重要意义。该文综述了β-半乳糖苷酶的微生物来源、特性、催化特性的研究现状,对适冷β-半乳糖苷酶的来源、特性、耐冷机制及工业化应用进行了系统阐述,并对其前景进行了展望。

关键词： β-半乳糖苷酶适冷β-半乳糖苷酶耐冷机制

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近视屈光参差儿童周边视网膜离焦状态分析

中华眼视光学与视觉科学杂志 2024年第6期26卷 416-422页

作者：廖俊屈雪梅张春芳熊燕何光华成都爱迪眼科医院成都

目的: 观察近视屈光参差儿童的周边视网膜相对离焦量(RPRE), 探索与近视发展相关的离焦区。方法: 系列病例对照研究。连续纳入2022年7—8月于成都爱迪眼科医院就诊的近视屈光参差儿童83例(166眼)。所有儿童在小瞳下使用生物测量仪测量... 详细信息

目的: 观察近视屈光参差儿童的周边视网膜相对离焦量(RPRE), 探索与近视发展相关的离焦区。方法: 系列病例对照研究。连续纳入2022年7—8月于成都爱迪眼科医院就诊的近视屈光参差儿童83例(166眼)。所有儿童在小瞳下使用生物测量仪测量双眼眼轴长度(AL)。在1%环喷脱脂滴眼液睫状肌麻痹后使用自动验光仪测量并计算双眼等效球镜度(SE)。使用屈光地形图(MRT)测量视网膜上、下、鼻、颞(RPRE-S、RPRE-I、RPRE-N、RPRE-T)共4个象限以及偏心0～15°、15°～30°、30°～45°、45°～53°共4个区域(相连区域的分界线数据归后一组)的相对屈光度。使用配对t检验比较左右双眼间不同象限、不同区域的RPRE差异, 并使用Spearman分析中心屈光度、AL与视网膜离焦状态的相关性。结果: 双眼RPRE在4个象限以及偏心15°～30°、30°～45°、45°～53°区域内差异均有统计学意义(t=3.09～8.04, 均P<0.05), 在偏心0～15°区域内差异无统计学意义(t=0.76, P=0.448)。双眼间的RPRE差值(D-RPRE)在4个象限间差异无统计学意义(F=1.84, P=0.140), 在偏心15°～30°、30°～45°、45°～53°区域间差异有统计学意义(F=14.49, P<0.001), 事后比较发现偏心15°～30°与偏心30°～45°和偏心45°～53°差异均有统计学意义(P=0.020, P<0.001), 偏心30°～45°与偏心45°～53°差异无统计学意义(P=0.270)。双眼间的SE差值(D-SE)分别与偏心30°～45°、45°～53°区域的D-RPRE呈负相关(r=-0.26, P=0.016;r=-0.32, P=0.003), 与偏心15°～30°区域的D-RPRE无相关性(r=-0.15, P=0.162)。双眼间的AL差值分别与偏心30°～45°、45°～53°区域的D-RPRE呈正相关(r=0.38, P<0.001;r=0.45, P<0.001), 而与偏心15°～30°区域的D-RPRE无相关性(r=0.19, P=0.090)。结论: 近视屈光参差儿童的近视发展与周边视网膜离焦状态有关, 偏心30°～53°的双眼间离焦量差异可能是其近视不平衡发展的促进因素之一。

关键词：近视屈光参差周边视网膜离焦屈光地形图

Some Characterizations of Bloch Functions

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Analysis in Theory and Applications 2018年第3期34卷 225-231页

作者： guanghua he College of International Finance and Trade Zhejiang Yuexiu University of Foreign Languages

We define Bloch-type functions of C;（D） on the unit disk of complex plane C and characterize them in terms of weighted Lipschitz functions. We also discuss the boundedness of a composition operator C;acting between ... 详细信息

关键词： Bloch space majorant composition operator