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Molecular Mechanisms of Intracellular Delivery of Nanoparticles Monitored by an Enzyme‑Induced Proximity Labeling

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Nano-Micro Letters 2024年第6期16卷 14-37页

作者： Junji Ren Zibin Zhang Shuo Geng Yuxi Cheng Huize Han Zhipu Fan Wenbing Dai Hua Zhang Xueqing Wang Qiang Zhang bing he Department of Pharmaceutics School of Pharmaceutical Sciences Peking University38 Xueyuan RdHaidian DistrictBeijing 100191People’s Republic of China

Achieving increasingly finely targeted drug delivery to organs,tissues,cells,and even to intracellular biomacromolecules is one of the core goals of *** the delivery destination is refined to cellular and subcellular targets,it is essential to explore the delivery of nanomedicines at the molecular ***,due to the lack of technical methods,the molecular mechanism of the intracellular delivery of nanomedicines remains unclear to ***,we develop an enzyme-induced proximity labeling technology in nanoparticles(nano-EPL)for the real-time monitoring of proteins that interact with intracellular ***(lactic-co-glycolic acid)nanoparticles coupled with horseradish peroxidase(HRP)were fabricated as a model(HRP(+)-PNPs)to evaluate the molecular mechanism of nano delivery in *** adding the labeling probe biotin-phenol and the catalytic substrate H_(2)O_(2)at different time points in cellular delivery,nano-EPL technology was validated for the real-time in situ labeling of proteins interacting with ***-EPL achieves the dynamic molecular profiling of 740 proteins to map the intracellular delivery of HRP(+)-PNPs in macrophages over *** on dynamic clustering analysis of these proteins,we further discovered that different organelles,including endosomes,lysosomes,the endoplasmic reticulum,and the Golgi apparatus,are involved in delivery with distinct participation *** importantly,the engagement of these organelles differentially affects the drug delivery efficiency,reflecting the spatial–temporal heterogeneity of nano delivery in *** summary,these findings highlight a significant methodological advance toward understanding the molecular mechanisms involved in the intracellular delivery of nanomedicines.

关键词： Enzyme-induced proximity labeling Intracellular delivery Nano-protein interaction Dynamic molecule profiling Macrophages

Photothermal‐boosted polaron transport in Fe_(2)O_(3)photoanodes for efficient photoelectrochemical water splitting

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Carbon Energy 2023年第9期5卷 38-48页

作者： Xiaoqin Hu Jing Huang Yu Cao bing he Xun Cui Yunhai Zhu Yang Wang Yihuang Chen Yingkui Yang Zhen Li Xueqin Liu State Key Laboratory of New Textile Materials and Advanced Processing Technologies Wuhan Textile UniversityWuhanHubeiChina Faculty of Materials Science and Chemistry China University of GeosciencesWuhanHubeiChina College of Chemistry and Materials Engineering Wenzhou UniversityWenzhouZhejiangChina

Introduction of the photothermal effect into transition-metal oxide photoanodes has been proven to be an effective method to improve the photoelectrochemical(PEC)water-splitting ***,the precise role of the photothermal effect on the PEC performance of photoanodes is still not well ***,spinel-structured ZnFe_(2)O_(4)nanoparticles are deposited on the surface of hematite(Fe_(2)O_(3)),and the ZnFe_(2)O_(4)/Fe_(2)O_(3)photoanode achieves a high photocurrent density of 3.17 mA cm^(-2)at 1.23 V versus a reversible hydrogen electrode(VRhe)due to the photothermal effect of ZnFe_(2)O_(4).Considering that the hopping of electron small polarons induced by oxygen vacancies is thermally activated,we clarify that the main reason for the enhanced PEC performance via the photothermal effect is the promoted mobility of electron small polarons that are bound to positively charged oxygen *** the synergistic effect of oxygen vacancies and the photothermal effect,the electron conductivity and PEC performance are significantly improved,which provide fundamental insights into the impact of the photothermal effect on the PEC performance of small polaron-type semiconductor photoanodes.

关键词： Fe_(2)O_(3)nanorods oxygen vacancies photoelectrochemical performance photothermal effect small polaron transport

Lipid-based nanoparticles for cancer immunotherapy

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Medical Review

Medical Review 2023年第3期3卷 230-269页

作者： Shumin Fan Huize Han Zhicheng Yan Yao Lu bing he Qiang Zhang Beijing Key Laboratory of Molecular Pharmaceutics and New Drug Delivery Systems State Key Laboratory of Natural and Biomimetic DrugsSchool of Pharmaceutical SciencesPeking UniversityBeijingChina Ningbo Institute of Marine Medicine Peking UniversityNingbo 315832Zhejiang ProvinceChina

As the fourth most important cancer management strategy except surgery, chemotherapy and radiotherapy, cancer immunotherapy has been confirmed to elicitdurable antitumor effects in the clinic by leveraging thepatient’s own immune system to eradicate the cancer ***, the limited population of patients who benefitfrom the current immunotherapies and the immune relatedadverse events hinder its development. The immunosuppressive microenvironment is the main cause of the failure,which leads to cancer immune evasion and immunity cycleblockade. Encouragingly, nanotechnology has been engineered to enhance the efficacy and reduce off-target toxicityof their therapeutic cargos by spatiotemporally controllingthe biodistribution and release kinetics. Among them, lipid-based nanoparticles are the first nanomedicines to makeclinical translation, which are now established platforms fordiverse areas. In this perspective, we discuss the availablelipid-based nanoparticles in research and market here, thendescribe their application in cancer immunotherapy, withspecial emphasis on the T cells-activated and macrophagestargeted delivery system. Through perpetuating each step ofcancer immunity cycle, lipid-based nanoparticles can reduceimmunosuppression and promote drug delivery to triggerrobust antitumor response.

关键词： cancer immunity cycle cancer immunotherapy lipid-based nanoparticles macrophages T cells

A spatiotemporal intelligent framework and experimental platform for urban digital twins

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Virtual Reality & Intelligent Hardware 2023年第3期5卷 213-231页

作者： Jinxing HU Zhihan LV Diping YUAN bing he Wenjiang CheN Xiongfei YE Donghao LI Ge YANG Shenzhen Institute of Advanced Technology Chinese Academy of SciencesShenzhen 518055China Department of Game Design Faculty of ArtsUppsala UniversityUppsala SE-75105Sweden Shenzhen Urban Public Safety and Technology Institute Co.Ltd Shenzhen 518172China

Backgrounds This work emphasizes the current research status of the urban Digital Twins to establish an intelligent spatiotemporal framework.A Geospatial Artificial Intelligent(GeoAI)system is developed based on the Geographic Information System and Artificial *** integrates multi-video technology and Virtual City in urban Digital *** Besides,an improved small object detection model is proposed:YOLOv5-Pyramid,and Siamese network video tracking models,namely MPSiam and FSSiamese,are ***,an experimental platform is built to verify the georeferencing correction scheme of video *** The MultiplyAccumulate value of MPSiam is 0.5B,and that of ResNet50-Siam is ***,the model is compressed by *** inference speed has increased by 3.3 times,reaching 83 Frames Per Second.3%of the Average Expectation Overlap is ***,the urban Digital Twins-oriented GeoAI framework established here has excellent performance for video georeferencing and target detection problems.

关键词： Spatiotemporal intelligence Urban digital twins Geographic information system Artificial intelligence Small target detection

STUDY ON SORPTION OF UREA BY MEANS OF MNTAL-POLYMER COMPLEXES

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Chinese Chemical Letters 1992年第8期3卷 669-672页

作者： bing Lin he Xiao bing ZHAO Institute of Polymer Chemistry,Nankai Universlty,Tianjin,300071

Urea can be sorbed by complexation with metal-polyaerylic acid *** experimental results indicate that the coordination occurs in the 0 atom of urea with Cu(Ⅱ)in Cu(Ⅱ)-polyaerylic acid complex and the sorption capaci... 详细信息

关键词： IC Cu 乞一 STUDY ON SORPTION OF UREA BY MEANS OF MNTAL-POLYMER COMPLEXES 台五

A signaling cascade mediating fruit trait development via phosphorylation-modulated nuclear accumulation of JAZ repressor

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Journal of Integrative Plant Biology 2024年第6期66卷 1106-1125页

作者： Wei Wang Jinyao Ouyang Yating Li Changsheng Zhai bing he Huahan Si Kunsong Chen Jocelyn K.C.Rose Wensuo Jia College of Horticulture China Agricultural UniversityBeijing 100193China College of Agriculture and Biotechnology Zhejiang UniversityZijingang CampusHangzhou 310058China Plant Biology Section School of Integrative Plant ScienceCornell UniversityIthaca14853NYUSA Institute of Horticulture Crops Xinjiang Academy of Agricultural SciencesUrumqi 830000China

It is generally accepted that jasmonate-ZIM domain(JAZ)repressors act to mediate jasmonate(JA)signaling via CORONATINE-INSENSITIVE1(COI1)-mediated ***,we report a cryptic signaling cascade where a JAZ repressor,FvJAZ12,mediates multiple signaling inputs via phosphorylation-modulated subcellular translocation rather than the COI1-mediated degradation mechanism in strawberry(Fragaria vesca).FvJAZ12 acts to regulate flavor metabolism and defense response,and was found to be the target of Fv MPK6,a mitogen-activated protein kinase that is capable of responding to multiple signal ***6 phosphorylates FvJAZ12 at the amino acid residues S179 and T183 adjacent to the PY residues,thereby attenuating its nuclear accumulation and relieving its repression for FvMYC2,which acts to control the expression of lipoxygenase 3(FvLOX3),an important gene involved in JA biosynthesis and a diverse array of cellular *** data reveal a previously unreported mechanism for JA signaling and decipher a signaling cascade that links multiple signaling inputs with fruit trait development.

关键词： defense response JAZ repressor phosphorylation strawberry subcellular translocation

Treating both wastewater and excess sludge with an innovative process

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Journal of Environmental Sciences 2003年第6期15卷 749-756页

作者： he Sheng-bing, WANG Bao-zhen, WANG Lin, JIANG Yi-feng (School of Municipal & Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China. School of Municipal & Environmental Engineering Harbin Institute of Technology Harbin 150090 China

The innovative process consists of biological unit for wastewater treatment and ozonation unit for excess sludge treatment. An aerobic membrane bioreactor(MBR) was used to remove organics and nitrogen, and an anaerobic reactor was added to the biological unit for the release of phosphorus contained at aerobic sludge to enhance the removal of phosphorus. For the excess sludge produced in the MBR, which was fed to ozone contact column and reacted with ozone, then the ozonated sludge was returned to the MBR for further biological treatment. Experimental results showed that this process could remove organics, nitrogen and phosphorus efficiently, and the removals for COD, NH 3-N, TN and TP were 93.17%, 97.57%, 82.77% and 79.5%, respectively. Batch test indicated that the specific nitrification rate and specific denitrification rate of the MBR were 1.03 mg NH 3-N/(gMLSS·h) and 0.56 mg NOx-N/(gMLSS·h), and denitrification seems to be the rate-limiting step. Under the test conditions, the sludge concentration in the MBR was kept at 5000—6000 mg/L, and the wasted sludge was ozonated at an ozone dosage of 0.10 kgO 3/kgSS. During the experimental period of two months, no excess sludge was wasted, and a zero withdrawal of excess sludge was implemented. Through economic analysis, it was found that an additional ozonation operating cost for treatment of both wastewater and excess sludge was only 0.045 RMB Yuan(USD 0.0054)/m 3 wastewater.

关键词： wastewater treatment excess sludge aerobic membrane bioreactor(MBR) anaerobic reactor ozonation zero withdrawal of excess sludge

Progress and perspective of the cathode/electrolyte interface construction in all-solid-state lithium batteries

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Carbon Energy 2021年第6期3卷 866-894页

作者： Shiming Su Jiabin Ma Liang Zhao Kui Lin Qidong Li Shasha Lv Feiyu Kang Yan-bing he Shenzhen Geim Graphene Center International Graduate School at ShenzhenTsinghua UniversityShenzhenChina Laboratory of Advanced Materials School of Materials Science and EngineeringTsinghua UniversityBeijingChina Key Laboratory of Beam Technology of Ministry of Education College of Nuclear Science and TechnologyBeijing Normal UniversityBeijingChina

Security risks of flammability and explosion represent major problems with the use of conventional lithium rechargeable batteries using a liquid *** application of solid-state electrolytes could effectively help to avoid these safety ***,integrating the solid-state electrolytes into the all-solid-state lithium batteries is still a huge challenge mainly due to the high interfacial resistance present in the entire battery,especially at the interface between the cathode and the solid-state electrolyte pellet and the interfaces inside the ***,recent progress made from investigations of cathode/solid-state electrolyte interfacial behaviors including the contact problem,the interlayer diffusion issue,the space-charge layer effect,and electrochemical compatibility is presented according to the classification of oxide-,sulfide-,and polymer-based solid-state *** also propose strategies for the construction of ideal next-generation cathode/solid-state electrolyte interfaces with high room-temperature ionic conductivity,stable interfacial contact during long cycling,free formation of the space-charge region,and good compatibility with high-voltage cathodes.

关键词： cathode configuration design interface lithium battery solid-state electrolyte

SIRT3 expression in hepatocellular carcinoma and its impact on proliferation and invasion of hepatoma cells

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Asian Pacific Journal of Tropical Medicine 2013年第8期6卷 649-652页

作者： Bo Zhang Lei Qin Cui-Jie Zhou Ye-Lu Liu Hai-Xin Qian Song-bing he The First Affiliated Hospital of Soochow University Zhangjiagang Hospital Affiliated of Soochow University Huai ' an First People ' s Hospital Nanjing Medical University

Objective: To observe expression of SIRT3 in normal liver tissue, cirrhotic tissue and hepatocellular carcinoma (HCC) tissues, and to explore the significance of SIRT3 in primary HCC . Methods: SIRT3 expression was detected in 10 normal cases, 30 cases with, 30 HCC cases by immunohistochemical and Western-blotting method. Results: Immunohistochemical assay showed that the SIRT3 positive expression rates were 100.0% (10/10), 96.7% (29/30) and 60.0% (18/30), respectively in normal group, paracancer group and HCC group. And the SIRT3 expression in HCC group was significantly lower than in normal group and paracancer group ( Phe SIRT3 expression in cancer tissue was 0.29暲0.07, significantly lower than that in paracancer group and normal group ( Phe differentiation degree and portal vein tumor thrombus ( Phe biological behavior of primary HCC.

关键词： SIRT3 Primary hepatocellular carcinoma Early stage Progress