检索结果-南通市图书馆

Characterization of multidrug-resistant Klebsiella pneumoniae isolated from the Chinese cobra Naja atra in a Beijing suburb

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

BIOCELL 2018年第2期42卷 47-54页

作者： Haifeng WANG hongxuan he School of Environmental Engineering Central Plains Specialty Food Engineering&Technology Research CenterYellow River Conservancy Technical InstituteDong Jing AvenueKaifengChina National Research Center for Wildlife-Borne Diseases Key Laboratory of Animal Ecology and Conservation BiologyInstitute of ZoologyChinese Academy of SciencesChina Kaifeng Key Laboratory of Food Quality Assessment KaifengChina

The emergence and spread of antibiotic resistance genes among Bacteria are a serious threat to global *** occurrence in animals which are in contact with humans is also *** Chinese cobra(Naja atra,Elapidae),though a highly venomous species,is appreciated as food and as a source of materials used in traditional Chinese *** are here reporting the isolation of multidrug-resistant Klebsiella pneumoniae(Enterobacteriaceae)from the lung of Naja atra,obtained from a snake farm in a Beijing *** study analyzed,using gene sequencing,the occurrence of antibiotic resistance genes(ARGs)in three *** isolates from two *** addition,bacterial clones were identified by biochemical tests and phylogenetic *** of antimicrobial susceptibility showed that all *** isolates were resistant to a host of antibiotics(piperacillin,cefazolin,gentamicin,tetracycline,doxycyclin,ciprofloxacin,levofloxacin,lomefloxacin,ofloxacin,norfloxacin,nalidixic acid,chloramphenicol,nitrofurantoin,sulfamethoxazole,and sulfamethoxazole/trimethoprim)but were susceptible to cefotaxime,cefixime,aztreonam,bramycin,amikacin,kanamycin,netilmicin,and *** ARGs were detected in total DNA extracted from the *** showed three quinolone resistance genes(oqxA,oqxB,qnrB),the gyrA gene that confers resistance to beta-lactam antibiotics,and the emerging aac(3)-II gene that confers resistance to *** is an important opportunistic human pathogen and the emergence of multidrug-resistant *** in *** suggests the increasing risk of pathogen transmission between humans,livestock,and *** the close association between foodborne pathogenic microorganisms and humans,it is key factor to identify these antibiotic resistance genes profile thereby minimize the risk of *** transmission.

关键词： Antimicrobial susceptibility Naja atra Resistance genes

Effect of epidemic diseases on wild animal conservation

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Integrative Zoology 2023年第6期18卷 963-980页

作者： Bin HU Shuyi HAN hongxuan he National Research Center for Wildlife-Borne Diseases Institute of ZoologyChinese Academy of SciencesBeijingChina College of Life Sciences University of Chinese Academy of SciencesBeijingChina

Under the background of global species extinction,the impact of epidemic diseases on wild animal protection is increasingly ***,we review and synthesize the literature on this topic,and discuss the relationship between diseases and *** usually reduce species diversity by decreasing or extinction of species populations,but also accelerate species evolution and promote species *** the same time,species diversity can regulate disease outbreaks through dilution or amplification *** synergistic effect of human activities and global change is emphasized,which further aggravates the complex relationship between biodiversity and ***,we emphasize the importance of active surveillance of wild animal diseases,which can protect wild animals from potential diseases,maintain population size and genetic variation,and reduce the damage of diseases to the balance of the whole ecosystem and human ***,we suggest that a background survey of wild animal populations and their pathogens should be carried out to assess the impact of potential outbreaks on the population or species *** mechanism of dilution and amplification effect between species diversity and diseases of wild animals should be further studied to provide a theoretical basis and technical support for human intervention measures to change *** importantly,we should closely combine the protection of wild animals with the establishment of an active surveillance,prevention,and control system for wild animal epidemics,in an effort to achieve a win-win situation between wild animal protection and disease control.

关键词： active surveillance amplification effect dilution effect disease spillover wild animal protection

Genetic characterizations of Toll-like receptors in the brown rat and their associations with pathogen infections

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Integrative Zoology 2022年第5期17卷 879-889页

作者： Qianqian SU Yi CheN Bo WANG Qingxun ZHANG hongxuan he National Research Center for Wildlife-Borne Diseases Chinese Academy of SciencesInstitute of ZoologyBeijingChina University of Chinese Academy of Sciences BeijingChina State Key Laboratory of Integrated Management of Pest Insects and Rodents Chinese Academy of SciencesInstitute of ZoologyBeijingChina

Toll-like receptors(TLRs)are important initiators of innate immune responses that target host-pathogen ***,further research into the molecular characteristics of TLRs in wild populations is required,as well as how TLRs genetically influenced pathogen infections in the brown rat(Rattus norvegicus).here,we explored the genetic characterization and evolution of 2 sensing nucleic acid TLRs(TLR7 and TLR8)and 2 sensing nonnucleic acid TLRs(TLR2 and TLR4)in the wild brown rat,and assessed their associations with 2 RNA viruses(Seoul hantavirus and rat hepatitis E virus(heV))and 2 bacteria(Leptospira and Bartonella).In these 4 TLRs,we discovered a total of 16 ***,TLR8 had high genetic diversity among 7 variants,while TLR2 had low genetic diversity with only 1 *** to selective pressure analyses,TLR4,TLR7,and TLR8 genes evolved under purifying ***,significant associations were found between 3 TLR8 variants and heV infection,as well as 1 TLR2 variant and Bartonella ***,our findings provided a glimpse into the genetic characterization of TLRs in the brown rat,and further demonstrated that TLR2 and TLR8 genetic variations were related to Bartonella and heV infection,***,TLR8 may be a good candidate immune gene for future research on molecular ecology and functional adaptation in wild populations.

关键词： Bartonella brown rats hepatitis E virus polymorphism susceptibility Toll-like receptors

Isolation and characterization of a Neisseria sp. from the captive wild goose (Anser anser)

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Integrative Zoology 2016年第2期11卷 128-133页

作者： Chengmin WANG Jing LUO Haijing WANG Said AMER Hua DING Ying DONG hongxuan he National Research Center For Wildlife Born Diseases Key Laboratory of Animal Ecology and Conservation BiologyInstitute of ZoologyChinese Academy of SciencesBeijingChina Department of Zoology Faculty of ScienceKafr El Sheikh UniversityKafr El SheikhEgypt Department of Infectious Diseases Hangzhou Center for Disease Control and PreventionHangzhouChina Department of Medical Oncology the Second Affiliated HospitalCollege of MedicineZhejiang UniversityHangzhouChina

The present study investigated 15 dead cases of captive wild goslings(Anser anser),which were bred in a small poultry farm in Shandong Province,*** examined cases presented diverse clinical signs accompanied with neurological manifestations and fatal *** culture identified the gram-negative Neisseria *** the brain homogenate of most examined cases(10/15,66.7%).The isolated bacteria were identified based on morphologic characteristics,biochemical tests and 16S rDNA *** proved that 1 identical bacterial strain(BNO09-3)was isolated from the positive *** phylogeny based on the 16S rDNA gene sequences indicated that this isolate has a close relationship with various strains of genus Neisseria *** from liver and feces of *** is the first report of Neisseria *** fatality in captive wild geese in China.

关键词： 16S rDNA Anser anser China Neisseria sp.

Molecular characterization of H1N1 influenza A viruses from human cases in North America

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2009年第13期54卷 2179-2192页

作者： WU Bin WANG ChengMin DONG GuoYing LUO Jing ZHAO BaoHua he hongxuan National Research Center For Wildlife Born Diseases Key Laboratory of Animal Ecology and Conservation Biology Institute of Zoology Chinese Academy of Sciences Beijing 100101 China Graduate University of Chinese Academy of Sciences Beijing 100049 China College of Life Science Hebei Normal University Shijiazhuang 050016 China

Subtypes of H1N1 influenza virus can be found in humans in North America,while they are also asso-ciated with the infection of *** of the genotypes of viral strains in human popula-tions is important to understand the source and distribution of viral *** and protein sequences of 10 isolates of the 2009 outbreak ofinfluenza A(H1N1) virus in North America were obtained from GenBank *** characterize the genotypes of these viruses,phylogenetic trees of genes PB2,PB1,PA,HA,NP,NA,NS and M were constructed by Phylip3.67 program and N-Linked glycosylation sites of HA,NA,PB2,NS1 and M2 proteins were analyzed online by NetNGlyc1.0 *** analysis indicated that these isolates are virtually identical but may be recombinant viruses because their genomic fragments come from different *** isolates also contain a char-acteristic lowly pathogenic amino acid motif at their HA cleavage sites(IPSIQSR↓GL),and an E residue at position 627 of the PB2 protein which shows its high affinity to *** homologous model of M proteins showed that the viruses had obtained the ability of anti-amantadine due to the mutation at the drug-sensitive site,while sequence analysis of NA proteins indicated that the viruses are still suscep-tible to the neuraminidase inhibitor drug(*** and zanamivir) because no mutations have been *** results strongly suggested that the viruses responsible for the 2009 outbreaks ofinfluenza A(H1N1) virus have the ability to cross species barriers to infect human and mammalian animals based on molecular *** findings may further facilitate the therapy and prevention of possible transmission from North America to other countries.

关键词： A型流感病毒分子鉴定北美病例人类

Yersinia pestis: examining wildlife plague surveillance in China and the USA

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Integrative Zoology 2012年第1期7卷 99-109页

作者： Sarah N.BEVINS John A.BAROCH Dale L.NOLTE Min ZHANG hongxuan he US Department of Agriculture Wildlife ServicesNational Wildlife Disease ProgramFort CollinsColoradoUSA Chinese Academy of Sciences Institute of ZoologyBeijingChina

Plague is a zoonotic disease caused by the bacterium Yersinia pestis Lehmann and Neumann,*** it is essentially a disease of rodents,plague can also be transmitted to ***,plague has caused mas-sive morbidity and mortality events in human populations,and has recently been classified as a reemerging dis-ease in many parts of the *** public health threat has led many countries to set up wild and domestic an-imal surveillance programs in an attempt to monitor plague activity that could potentially spill over into human *** China and the USA have plague surveillance programs in place,but the disease dynamics dif-fer in each *** present data on plague seroprevalence in wildlife and review different approaches for plague surveillance in the 2 *** need to better comprehend plague dynamics,combined with the fact that there are still several thousand human plague cases per year,make well-designed wildlife surveillance pro-grams a critical part of both understanding plague risks to humans and preventing disease outbreaks in the future.

关键词： plague sentinel species surveillance Yersinia pestis

Detection of new antibiotic resistance gene profile in Escherichia coliassociated with avian leukosis virus infection from broiler chickens

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

BIOCELL 2020年第2期44卷 217-224页

作者： HAIFENG WANG JUAN GUO LIJING ZheNG SHUYING LIU ZheRONG WANG hongxuan he School of Environmental Engineering Central Plains Specialty Food Engineering and Technology Research CenterKaifeng Key Laboratory of Food Composition and Quality AssessmentYellow River Conservancy Technical InstituteKaifeng475004China National Research Center for Wildlife-Borne Diseases Key Laboratory of Animal Ecology and Conservation BiologyInstitute of ZoologyChinese Academy of SciencesBeijingChina

The Escherichia coli(***)is prevailing worldwide,but the epidemiology of *** infections feature regional distribution characteristics to some ***,as a zoonotic pathogen,can be transferred from animals to humans through food chain or via contact with wounds,causing a public health *** reported the swelling of proventriculus and tracheal bleeding following the death in two broiler chickens(Gallus gallus domesticus)from Beijing,*** investigate whether a virus was involved in the infection,Madin Darby Bovine Kidney(MDCK)cells were co-cultured with supernatants of proventriculus,trachea and spleen *** avian leucosis virus was detected in the samples of proventriculus and trachea,but the avian influenza virus,the Newcastle disease virus and the avian infectious laryngotracheitis virus were not *** isolates were resistant to almost all the antimicrobial as tested except for the combinations of amoxicillin/clavulanic acid and sulfamethoxazole/*** tests demonstrated the presence of antibiotic resistance genes in these *** isolates and further research revealed a novel gene profile with the presence of CTX-M-1,gyrA,gyrB,oqxA,oqxB,parC and Sul2 antibiotic resistance genes in a strain isolated from a proventriculus *** results demonstrated that the presence of antibiotic resistant *** would not necessarily cause outbreak of large-scale ***,when the bacteria carrying new antibiotic resistance genes enter the environment,it may result in the development of more virulent strains which will potentially impact human and animal health.

关键词： Patterns E coli Poultry Genes

Qualitative and quantitative analyses of influenza virus receptors in trachea and lung tissues of humans,mice,chickens and ducks

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2012年第7期55卷 612-617页

作者： WANG BaoLin LIU Bo CheN Ling ZHANG JianYong he hongxuan ZHANG Hong Laboratory of Respiratory Medicine Department of Respiratory Medicine Affiliated Hospital of Zunyi Medical College Zunyi 563003 China National Research Center for Wildlife-borne Diseases Institute of Zoology Chinese Academy af Sciences Beijing 100101 China Z-BioMed Inc. Rockville MD 20855 USA

To accurately determine the expression and distribution patterns of two infuenza virus receptors （SAa2,3-gal and SAa2,6-gal） in trachea and lung tissues of humans, mice, chickens and ducks, we analyzed lectin immunofluorescence stainings of various tissue sections qualitatively and quantitatively. Results from the qualitative analysis showed that both influenza virus receptors were expressed in lung tissues of humans, mice, chickens and ducks as well as trachea tissues of mice and ducks. However, SAa2,6-gal receptor was expressed only in the human trachea tissue and SAa2,3-gal receptor was expressed only in the chicken trachea tissue. Results from the quantitative analysis demonstrated that both receptors were expressed in trachea tis- sues of human and mouse, as well as in lung tissues of humans, chickens and ducks. Meanwhile, our results also showed that the expression and distribution of influenza virus receptors in the same tissue were not always uniform, indicating that their distribution and expression in various tissues are not simply the distinction between the presence or absence of receptors, but rather the difference in the amount of expressed receptors.

关键词： influenza virus siaUc acid receptors trachea lung lectin immunofluorescence quantitative analysis qualitative analysis

Identification and genetic characterization of Contracaecum sp.(Nematoda:Anisakidae)from China

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Integrative Zoology 2021年第6期16卷 929-938页

作者： Qingxun ZHANG Meng MENG Chengmei HUANG Shengyong FENG Jie LIU Yan TANG Yu FAN Guohui YUAN Shuyi HAN Jing LUO Baohua ZHAO hongxuan he National Research Center for Wildlife-Borne Diseases Institute of ZoologyChinese Academy of SciencesBeijingChina University of Chinese Academy of Sciences BeijingChina College of Wildlife Resources Northeast Forestry UniversityHaerbinHeilongjiangChina China Wildlife Conservation Association BeijingChina hebei Normal University ShijiazhuangHebeiChina

Contracaecum species are economically important fish-borne larval nematodes with zoonotic *** June 2019,more than 100 piscivorous birds died in their habitats close to the Wild Duck Lake,located in Yanqing,northwest of Beijing,***-mortem examination of Black Night herons(Nycticorax)revealed the presence of numerous anisakid nematodes in the *** nematodes were identified as Contracaecum *** on morphological *** analysis of the mitochondrial(mt)genome and the ITS gene showed that sequences of Contracaecum *** isolates were grouped into a new individual ***,the parasite was successfully isolated from fresh dead birds,feces of piscivorous birds,and fish and prevalence ranged from 8.0%to 81.8%.Consequently,our study demonstrated Contracaecum *** in different sources from China,which might constitute a threat to wildlife,aquaculture,and public health.

关键词： Anisakid epidemiology morphology phylogenetic analysis