检索结果-南通市图书馆

Biological Fingerprinting Analysis of Interaction Between Taxoids in Taxus and Microtubule Protein by Microdialysis Coupled with High-performance Liquid Chromatography/Mass Spectrometry for Screening Antimicrotubule Agents

引用

Chemical Research in Chinese Universities 2008年第4期24卷 411-419页

作者： LEI Xiao-yuan KONG Liang SU Xing-ye GUO Ming ZOU han-fa National Chromatographic R＆A Center Dalian Institute of Chemical Physics Chinese Academy of Sciences Dalian 116023 P. R. China College of Marine Environmental Engineering Dalian Fisheries University Dalian 116023 P R. China Department of Chemistry and Chemical Technology Dalian University Dalian 116622 P. R. China

Some natural products, such as traditional Chinese medicines（TCMs）, contain compounds with anticancer activity and have attracted a great interest in recent years as alternative anticancer therapies. A quick and convenient assay for screening antimicrotubule compounds in which in vitro microdialysis/high-performance liquid chromatography （HPLC） is used to monitor the binding of the compounds extracted from TCM Taxus cuspidata Siebold ＆ Zucc（Taxus） to microtubules is reported. It was observed that the extract of Taxus contains at least five compounds which have affinity interaction with microtubules by biological fingerprinting analysis, and they were identified as the taxoids of taxol, baccatin III, 10-deacetylbaccatin Ⅲ（10-DAB）, cephalomannine and 7-epi-10-deacetyltaxol （7-epi-10-DAT） based on the comparison of their high-performance liquid chromatographic/mass spectrometric and UV spectra with those of the standard samples, both assembly-promoting and disassembly-inhibiting characteristics of those compounds were evaluated. It was observed that baccatin Ⅲ and 10-DAB bound to microtubules and the binding degrees were influenced by GTP. Competitive binding behavior of taxol with other four taxoids to microtubules was also investigated.

关键词： Microdialysis/high-performance liquid chromatography(HPLC) Biological fingerprinting analysis Taxoids Microtubule Taxus

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Knots and Polynomials

引用

Journal of Mathematical Research and Exposition 2010年第2期30卷 257-264页

作者： You fa han Xiao Sha MA School of Mathematics Liaoning Normal University Liaoning 116029 P. R. China

In this paper, we deal with some corresponding relations between knots and polynomials by using the basic properties of knot polynomials （such as, some special values of knot polynomials, the Arf invariant and derivative of knot polynomials）. We give necessary and sufficient conditions that a Laurent polynomial with integer coefficients, whose breadth is less than five, is the Jones polynomial of a certain knot.

关键词： knot Jones polynomial Laurent polynomial.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Alterations to biological soil crusts with alpine meadow retrogressive succession affect seeds germination of three plant species

引用

Journal of Mountain Science 2016年第11期13卷 1995-2005页

作者： LI Yi-kang OUYANG Jing-zheng LIN Li XU Xing-liang ZhanG fa-wei DU Yan-gong LIU Shu-li CAO Guang-min han fa Key Laboratory of Adaptation and Evolution of Plateau Biota Northwest Institute of Plateau BiologyChinese Academy of SciencesXining 810001China University of Chinese Academy of Sciences Beijing 100049China Institute of Geographic Sciences&Natural Resources Research the Chinese Academy of SciencesBeijing 100101China Liancheng National Nature Reserve of Gansu Province Yongdeng Country 730000China

Biological soil crusts(BSCs) are the important components of alpine meadow *** extent and morphology of BSCs vary greatly with alpine meadow retrogressive succession due to grazing *** is significant interest in impacts of crust composition on plant seed germination,especial l y in(semi-) arid ***,little is known about the influences of BSCs,and their associations with alpine meadow succession,on germination of typical alpine meadow vascular plant *** a full factorial common-gardenexperiment,we studied effects of:(1) crust type,(2) seed position,and(3) surface texture on seed *** chose three typical alpine meadow plant species(*** pratensis,Tibetia himalaica and Potentillen nivea),which belonged to different functional groups(graminoids,legumes,and forbs) and play important roles in all alpine meadow succession *** type and seed position influenced seed germination,and the inhibitory effects of BSCs depended on the crust type and seed species *** major factors influencing seed germination were BSC type,seed position,soil texture,and the interactions between BSC type and seed position; species and seed position; species andsurface texture; and species,crust type,and surface *** crust significantly inhibited germination of all *** position also had a significant effect on seed germination(p face than below the surface,this was especially true for *** within cyanobacteria and lichen *** germination rates of *** on the soil surface were significantly correlated with plant occurrence frequency within the alpine meadow *** poor correlation for the other two species is possibly that they are *** results clearly demonstrated that BSCs can be biological filters during seed germination,depending on the BSC succession *** their influences on seed germination,BSCs can strongly

关键词： Crust type Seed position Microenvironment Germination Tibetan Plateau Vascular plants

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Expert systems for HPLC in biochemistry—The knowledge base for selection of HPLC column system in the separation of amino acids, peptides and proteins

引用

Chinese Journal of Chemistry 1991年第5期9卷 441-447页

作者： ZhanG Yu-Kui ZOU han-fa LU Pei-Zhang YAO Zhi-Jian Dalian Chromatographic R.&D.Center of China Dalian nstitute of Chemical PhysicsAcademia SinicaDalian 116011 Laboratory of Protein Chemistry and Biotechnology Institute of Basic Medical SciencesBeijing 100850

The strategy of expert system for high performance liquid chromatography was discussed, the attentions are mainly placed on the knowledge base for selection of column system, separation modes and detection modes in th... 详细信息

关键词： The knowledge base for selection of HPLC column system in the separation of amino acids Expert systems for HPLC in biochemistry peptides and proteins base HPLC

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Seismic damage zone and width-throw scaling along the strike-slip faults in the Ordovician carbonates in the Tarim Basin

引用

Petroleum Science 2019年第4期16卷 752-762页

作者： De-Bo Ma Guang-Hui Wu Nicola Scarselli Xin-Sheng Luo Jian-fa han Zhi-Yong Chen School of Geoscience and Technology Southwest Petroleum UniversityChengdu 610500SichuanChina PetroChina Research Institute of Petroleum Exploration and Development Beijing 100083China fault Dynamics Research Group Department of Earth SciencesRoyal Holloway University of LondonEgham TW20 0EXUK PetroChina Tarim Oilfield Company Korla 841000XinjiangChina

Understanding the scaling relation of damage zone width with displacement of faults is important for predicting subsurface faulting mechanisms and fluid flow processes. The understanding of this scaling relationship is influenced by the accuracy of the methods and types of data utilized to investigate faults. In this study, seismic reflection data are used to investigate the throw and damage zone width of five strike-slip faults a ecting Ordovician carbonates of the Tarim intracraton basin,NW China. The results indicate that fault slips with a throw less than 200 m had formed wide damage zones up to 3000 m in width. Also, damage zone width is found to have both a positive correlation and a power-law relation with throw of two orders of magnitude, with a ratio of these values varying in a range of 2–15. The relationship between throw and damage zone width is not a simple power-law and changes its slope from small to larger size faults. The results indicate that throw scales well with damage zone width for the studied faults, and hence these can be used to predict fault geometries in the Tarim Basin. The study of the wide carbonate damage zones presented here provides new insights into scaling of large-size faults, which involve multiple faulting stages.

关键词： fault damage zone Carbonate Throw Scaling Evolution Tarim Basin

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Active immunization with GnRH-tandem-dimer peptide in young male rats reduces serum reproductive hormone concentrations, testicular development and spermatogenesis

引用

Asian Journal of Andrology 2016年第3期18卷 485-491,I0012页

作者： Xing-fa han Jun-Li Li Yu-Qin Zhou Xiao-Hua Ren Gong-Cheng Liu Xiao-han Cao Xiao-Gang Du Xian-Yin Zeng Isotope Research Biological Engineering and Application Biology Department Laboratory Sichuan Agricultural University Ya'an 625014 People's Republic of China.

GnRH sterilization vaccines have been developed for various practical and clinical reasons. However, conjugation of GnRH peptide to carrier protein has many drawbacks, hampering the further commercialization of GnRH vaccines. In this study, a new nonconjugated GnRH vaccine, D-Lys6-GnRH-tandem-dimer peptide （TDK）, emulsified in Specol adjuvant was investigated for its immunocastration efficacy in young male rats. Prepubertal male rats were randomly allocated into three groups （n = 12）： control （no treatment）, surgically castrated or immunized against 100 μg TDK in Specol adjuvant at 6 weeks of age （with a booster 8 weeks later）. Blood samples （for antibody titers and hormone concentrations） were collected at 2-week intervals until rats were killed （18 weeks of age）. Compared to intact controls, active immunization against TDK reduced （P〈 0.05） serum concentrations of testosterone, inhibin B, LH and FSH, prevented the onset of spermatogenesis at puberty. Furthermore, mRNA expressions of GnRH receptor, LH-β and FSH-β in the pituitary, LH receptor, FSH receptor, inhibin α, βA and βB subunit in the testes were decreased in immunocastrated rats compared to intact controls （P 〈 0.05）. These results demonstrate for the first time that GnRH-tandem-dimer peptide emulsified in Specol is a promising veterinary sterilization medicine.

关键词： GnRH nonconjugation rat reproductive axis sterilization

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Spanning Pre-disks in a Compression Body

引用

Communications in Mathematical Research 2018年第2期34卷 177-183页

作者： Liang Liang han You-fa Li Feng-ling Zhao Lu-ying School of Mathematics Liaoning Normal University School of Mathematical Sciences Dalian University of Technology

A properly embedded essential planar surface P（not a disk） in a compression body V is called a spanning pre-disk with respect to J, if one boundary component of P is lying in ?;V and all other boundary components of P are lying in?;V and coplanar with J. In this paper, we show that the number of boundary components of spanning pre-disks in a compression body is unbounded. But the number of a maximal collection of spanning pre-disks is bounded.

关键词： spanning pre-disk curve complex compression body maximal collection

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effect of ligand of peroxisome proliferator-activated receptor γ on the biological characters of hepatic stellate cells

引用

World Journal of Gastroenterology 2005年第30期11卷 4735-4739页

作者： Yan-Tong Guo Xi-Sheng Leng Tao Li Ji-Run Peng Sheng-han Song Liang-fa Xiong Zhi-Zhong Qin Department of General Surgery Peking University People's Hospital Beijing 100044 China

AIM： To study the effect of rosiglitazone, which is a ligand of peroxisome proliferator-activated receptor gamma （PPARy）, on the expression of PPARy in hepatic stellate cells （HSCs） and on the biological characteristics of HSCs. METHODS： The activated HSCs were divided into three groups： control group, 3 μmol/L rosiglitazone group, and 10 μmol/L rosiglitazone group. The expression of PPARγ, α-smooth muscle actin （α-SMA）, and type Ⅰ and Ⅲ collagen was detected by RT-PCR, Western blot and immunocytochemical staining, respectively. Cell proliferation was determined with methylthiazolyltetrazolium （MTT） colodmetric assay. Cell apoptosis was demonstrated with flow cytometry. RESULTS： The expression of PPARγ at mRNA and protein level markedly increased in HSCs of 10 μmol/L rosiglitazone group （tvalue was 10.870 and 4.627 respectively, P〈0.01 in both）. The proliferation of HSCs in 10 μmol/L rosiglitazone group decreased significantly （t = 5.542, P〈0.01）, α-SMA expression level and type Ⅰ collagen synthesis ability were also reduced VS controls （tvalue = 10.256 and 14.627 respectively, P〈0.01 in both）. The apoptotic rate of HSCs significantly increased in 10 μmol/L rosiglitazone group vs control （X^2= 16.682, P〈0.01）. CONCLUSION： By increasing expression of PPARγ in activated HSCs, rosiglitazone, an agonist of PPARγ, decreases α-SNA expression and type Ⅰ collagen synthesis, inhibits cell proliferation, and induces cell apoptosis.

关键词： Peroxisome proliferator-activated receptor gamma Hepatic stellate cell Rosiglitazone

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

GEOLOGICAL AND GEOCHEMICAL FEATURES OF SUBMARINE VOLCANIC HYDROTHERMAL-SEDIMENTARY MINERALIZATION OF MAKENG IRON DEPOSIT,FUJIAN PROVINCE

GEOLOGICAL AND GEOCHEMICAL FEATURES OF SUBMARINE VOLCANIC HY...

引用

作者： han fa and Ge Chaohua

＜正＞～～

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Evolution of Proinflammatory Cytokines in Hepatocellular Carcinoma Patients Undergoing Orthotopic Liver Transplantation

引用

Clinical oncology and cancer researeh 2011年第1期8卷 38-41页

作者： Yu-liang WANG Yu ZhanG Zhi-jun ZHU Rui-fa han Tianjin Institute of Urology Tianjin Medical University Tianjin 300211 China Department of Anesthesia Tianjin Medical University Cancer Institute and Hospital Tianjin 300060 China Orient Organ Transplant Center First Central Clinic Institute of Tianjin Medical University Key Lab for Critical Care Medicine of the Ministry of Health Tianjin 300192 China.

OBJECTIVE To analyze the expression and levels of serum proinflammatory cytokines including tumor necrosis factor alpha （TNF-α）, and interleukin （IL）-6 in patients with hepatocellular carcinoma （HCC）, who received orthotopic liver transplantation （OLT）.METHODS The blood samples of 20 consecutive HCC patients who underwent liver transplantation were detected and analyzed for the clinical serum biochemical parameters, TNF-α and *** samples were drawn from the radial artery at planned time points： preoperatively, intraoperatively, and *** of serum TNF-α and IL-6 were detected with enzymelinked immunosorbent assay （ELISA）.RESULTS The levels of serum TNF-αand IL-6 increased significantly at reperfusion phase compared with those detected preoperatively （P 〈 0.01）, and the level of serum IL-6 remained significantly higher until the third day after the liver transplantation. There was a significant correlation between TNF-αand IL-6 （P〈0.001）.CONCLUSION This research into the effects of the proinflammatory cytokines on liver transplantation has provided new insights into the mechanisms of ischemia and reperfusion injury to OLT.

关键词： liver transplantation carcinoma hepatocellular reperfusion cytokines

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：