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The molecular mechanism of embryonic stem cell pluripotency maintenance

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Chinese Science Bulletin 2005年第19期50卷 2121-2131页

作者： WANG Qingzhong LIU Yixun han chunsheng College of Life Science Shandong Normal University Jinan 250014 China State Key Laboratory of Reproductive Biology Institute of Zoology Chinese Academy of Sciences Beijing 100080 China

In vitro cultured embryonic stem (ES) cells are derived from the inner cell mass (ICM) of pre-implantation embryos, and are capable of giving rise to all cell and tissue types of the three germ layers upon being injected back into blastocysts. These cells are therefore said to possess pluripo-tency that can be maintained infinitely in culture under op-timal conditions. Such pluripotency maintenance is believed to be due to the symmetrical cleavage of the cells in an un-differentiated state. The pluripotency of ES cells is the basis for their various practical and potential applications. ES cells can be used as donor cells to generate knockout or transgenic animals, as in vitro models of mammalian development, and as cell resources for cell therapy in regenerative medicine. The further success in these applications, particularly in the last two, is dependent on the establishment of a culture sys-tem with components in the medium clearly defined and the subsequent procedures for controlled differentiation of the cells into specific lineages. In turn, elucidating the molecular mechanism for pluripotency maintenance of ES cells is the prerequisite. This paper summarizes the recent progresses in this area, focusing mainly on the LIF/STAT3, BMPs/Smads, canonical Wnt, TGFβ/activin/nodal, PI3K and FGF signaling pathways and the genes such as oct4, nanog that are crucial in ES cell pluripotency maintenance. The regulatory systems of pluripotency maintenance in both mouse and human ES cells are also discussed. We believe that the cross-talkings between these signaling pathways, as well as the regulatory system underlying pluripotency maintenance will be the main focus in the area of ES cell researches in the future.

关键词：胚胎干细胞内部细胞聚集无限扩展微生物层对称分裂

脊髓圆锥弥散张量成像扫描在下尿路功能障碍中的应用

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中国康复理论与实践 2024年第3期30卷 333-338页

作者：孙浩瑜高轶吴娟廖利民靖华芳张思雨李东韩春生首都医科大学康复医学院北京市100068 中国康复研究中心北京博爱医院北京市100068 中国康复科学所北京市100068 北京市神经损伤与康复重点实验室北京市100068

目的利用MRI弥散张量成像(DTI)技术寻找膀胱过度活动症(OAB)和膀胱活动低下(UAB)患者脊髓圆锥信号的异常。方法 2021年5月至2023年4月,从北京博爱医院招募下尿路功能障碍患者23例(病例组)。所有患者行盆底电生理检查和影像尿动力检查,... 详细信息

目的利用MRI弥散张量成像(DTI)技术寻找膀胱过度活动症(OAB)和膀胱活动低下(UAB)患者脊髓圆锥信号的异常。方法 2021年5月至2023年4月,从北京博爱医院招募下尿路功能障碍患者23例(病例组)。所有患者行盆底电生理检查和影像尿动力检查,根据患者尿动力灌注期膀胱收缩情况,将病例组分为UAB组和OAB组。同时招募健康受试者8例(对照组)。所有受试者行T_(10)~L_(5)脊髓MRI平扫+DTI序列扫描。通过DTI序列对比MRI平扫节段确定脊髓圆锥位置,提取圆锥中段的各向异性分数(FA)、表观弥散系数(ADC)和相对各项异性(RA)。结果 UAB组12例,OAB组11例。病例组盆底电生理检查均存在异常。盆底电生理检查结果显示,UAB组与OAB组间骶反射弧神经传导有显著性差异(P=0.036)。DTI扫描结果显示,与对照组比较,病例组ADC升高(t=2.185, P=0.037);UAB组FA降低(t=3.439, P=0.005),ADC显著升高(t=4.582, P <0.001)。结论对于下尿路功能障碍患者,DTI有助于寻找脊髓是否存在潜在的病变。FA和ADC是很有价值的脊髓圆锥损伤的诊断指标。

关键词：膀胱过度活动症膀胱活动低下脊髓圆锥弥散张量成像

FAK-targeting PROTAC as a chemical tool for the investigation of non-enzymatic FAK function in mice

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Protein & Cell 2020年第7期11卷 534-539页

作者： Hongying Gao Chunwei Zheng Jian Du Yue Wu Yonghui Sun chunsheng han Kehkooi Kee Yu Rao MOE Key Laboratory of Protein Sciences School of Pharmaceutical SciencesMOE Key Laboratory of Bioorganic Phosphorus Chemistry Chemical BiologyTsinghua UniversityBeijing 100084.China Tsinghua University-Peking University Joint Center for Life Sciences Bejing 100084China State Key Laboratory of Stem Cell and Reproductive Biclogy Institute of ZoologyChinese Academy of SciencesBeijing 100084China Center for Stem Cell Biology and Regenerative Medicine Department of Basic Medical SciencesSchool of MedicineTsinghua UniversityBeijing 100084China University of Chinese Academy of Sciences Savaid Medical SchoolBejing 100049China

Dear Editor,Animal models,most commonly mice,that lack a protein of interest play an important role in phenotypic and functional studies of a target gene,allowing researchers to answer various biological questions(Chaible et al,2010).At pre-sent,a variety of tools act at the DNA or RNA level to enable researchers to model gene function(and thus protein)deficiency,including nucleic acid based RNA interference(EI-bashir et al.,2001),antisense oligonucleotides(Schoch and Miller,2017),and genome editing-based CRISPR-Cas9(Doudna and Charpentier,2014)***,challenges remain.

关键词： al. function enzymatic

Value of procalcitonin and presepsin in the diagnosis and severity stratification of sepsis and septic shock

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World Journal of Emergency Medicine 2024年第2期15卷 135-138页

作者： Enfeng Ren Hongli Xiao Guoxing Wang Yongzhen Zhao han Yu chunsheng Li Department of Emergency Medicine Beijing Friendship HospitalCapital Medical UniversityBeijing 100050China Department of Emergency Medicine Beijing Chaoyang HospitalCapital Medical UniversityBeijing 100050China

Sepsis is defined as life-threatening organ dysfunction caused by a dysregulated host response to infection.[1,2]Septic shock,the most severe form of sepsis,is characterized by circulatory and cellular/metabolic abnormalities,and can increase mortality to>40%.[1-3]Early recognition and risk stratification of septic shock are crucial but challenging because of the heterogeneity of its presentation and progression.

关键词： diagnosis sepsis mortality

Modeling Meiosis Initiation Using Mouse Spermatogonial Stem Cells In Vitro

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Modeling Meiosis Initiation Using Mouse Spermatogonial Stem ...

Enhancing cartilage regeneration and repair through bioactive and biomechanical modification of 3D acellular dermal matrix

中国生理学会生殖科学专业委员会—中国动物学会生殖生物学分会第二次联合学术年会暨“生殖科学专业委员会第二次学术交流会”和“生殖生物学分会第十六次学术年会”

作者： Si Wang Jian Chen chunsheng han State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of Sciences

We have recently reported that retinoic acid(RA)is sufficient for inducing the generation of leptotene/zygotene spermatocytes from cultured mouse spermatogonial stem cells(mSSCs).RA in combination with pup Sertoli cell co-culture results in an induction efficiency of 28%.We have used this model to study the roles of miR-202 in spermatogenesis and showed that it contributed to

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Regenerative Biomaterials 2024年第3期3卷 105-121页

作者： Wei Gao Tan Cheng Zhengya Tang Wenqiang Zhang Yong Xui Min han Guangdong Zhou chunsheng Tao Ning Xu Huitang Xia Weijie Sun Qingdao Medical College of Qingdao University Qingdao266071China Department of Infectious Diseases The First Affliated Hospital of Anhui Medical UniversityShushanHefei230022China Department of Orthopaedic Surgery Shanghai Sixth People's Hospital Affliated to Shanghai jiao Tong University School of MedicineShanghai200025China Department of Orthopedic Surgery Shanghai Eighth People's HospitalShanghai200235China Department of PlasticSurgery&Jinan ClinicalResearch Center for Tissue Engineering Skin Regeneration and Wound Repair The First Affliated Hospital of Shandong First Medical UniversityJinan266299China Department of Orthopaedics Ninety-seventh Hospital of the Chinese People's Liberation Army NavyQingdao266071China Department of Orthopaedics The First Affliated Hospital of Shandong First Medical UniversityJinan266299China Department of Plastic surgery Shanghai Key Laboratory of Tissue EngineeringShanghai Ninth People's HospitalShanghai jiao Tong University School of MedicineShanghai200023China Department of Thoracic Surgery Shanghai Children's HospitalShanghai Jiao Tong University School of MedicineShaghai200040China Department of Thoracic Surgery Shanghai Pulmonary HospitalSchool of MedicineTongji UniversityShanghai200433China

Acellular dermal matrix(ADM)shows promise for cartilage regeneration and ***,an effective decellularization technique that removes cellular components while preserving the extracellular matrix,the transformation of 2D-ADM into a suitable 3D scaffold with porosity and the enhancement of bioactive and biomechanical properties in the 3D-ADM scaffold are yet to be fully *** this study,we present an innovative decellularization method involving 0.125%trypsin and 0.5%SDS and a 1%Triton X-100 solution for preparing ADM and converting 2D-ADM into 3D-ADM *** scaffolds exhibit favorable physicochemical properties,exceptional biocompatibility and significant potential for driving cartilage regeneration in vitro and in *** further enhance the cartilage regeneration potential of 3D-ADM *** incorporated porcine-derived small intestinal submucosa(SIS)for bioactivity and calcium sulfate hemihydrate(CSH)for biomechanical *** resulting 3D-ADM+SIS scaffolds displayed heightened biological activity,while the 3D-ADM+CSH scaffolds notably bolstered biomechanical *** scaffold types showed promise for cartilage regeneration and repair in vitro and in vivo,with considerable improvements observed in repairing cartilage defects within a rabbit articular cartilage *** summary,this research introduces a versatile 3D-ADM scaffold with customizable bioactive and biomechanical properties,poised to revolutionize the field of cartilageregeneration.

关键词： acellular dermal matrix three-dimensional scaffolds cartilage regeneration small intestinal submucosa calcium sulfate hemihydrate

Faculty positions: Center for Agricultural Resources Research, Chinese Academy of Sciences

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中国生态农业学报 2014年第6期22卷 654-654页

作者： Dr. Yibo han chunsheng Hu Co-Chair of the Research Committee Center for Agricultural Resources ResearchInstitute of Genetics and Developmental BiologyChinese Academy of SciencesShijiazhuang Hebei 050022 China

The Center for Agricultural Resources Research(CARR),the Institute of Genetics and Developmental Biology(IGDB),Chinese Academy of Sciences,invites applicants for several research group leader *** is one of the research organizations in Chinese Academy of Sciences(CAS).We seek nominations and applications from individuals who have expertise and a record of accomplishment in research areas related to ecology,agro-hydrology,

关键词：农业生物技术遥感技术微生物

Diversity Analysis of Intestinal Microflora between Healthy and Diarrheal Neonatal Piglets in Jiangxi

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Diversity Analysis of Intestinal Microflora between Healthy ...

Combination therapy with Adenoviral-mediated PTEN and inhibitor of PI3K suppressed malignant glioma cell growth through regulating PI3K/AKT signaling pathway in vitro and in vivo

中国畜牧兽医学会动物微生态学分会第五届第十三次全国学术研讨会

作者： chunsheng han Yimin Dai Baosheng Liu Linkang Wang Jingqi Wang Jinhua Zhang College of Animal Science and Technology Jiangxi Agriculture University

Introduction Intestinal microflora exerts great influences on the host’s *** gut diseases are closely related to the change of the microbial *** neonatal piglet diarrhea causes significant economic losses,the microbiome composition changes remain *** this study,our goals are to find the intestinal microbial composition differences between the healthy and diarrheal neonatal piglets,even the

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Combination therapy with Adenoviral-mediated PTEN and inhibi...