FAK-targeting PROTAC as a chemical tool for the investigation of non-enzymatic FAK function in mice

作     者：Hongying Gao Chunwei Zheng Jian Du Yue Wu Yonghui Sun Chunsheng Han Kehkooi Kee Yu Rao Hongying Gao;Chunwei Zheng;Jian Du;Yue Wu;Yonghui Sun;Chunsheng Han;Kehkooi Kee;Yu Rao

作者机构：MOE Key Laboratory of Protein SciencesSchool of Pharmaceutical SciencesMOE Key Laboratory of Bioorganic Phosphorus Chemistry Chemical BiologyTsinghua UniversityBeijing 100084.China Tsinghua University-Peking University Joint Center for Life SciencesBejing 100084China State Key Laboratory of Stem Cell and Reproductive BiclogyInstitute of ZoologyChinese Academy of SciencesBeijing 100084China Center for Stem Cell Biology and Regenerative MedicineDepartment of Basic Medical SciencesSchool of MedicineTsinghua UniversityBeijing 100084China University of Chinese Academy of SciencesSavaid Medical SchoolBejing 100049China 

出 版 物：《Protein & Cell》 (蛋白质与细胞（英文版）)

年 卷 期：2020年第11卷第7期

页      面：534-539页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 

基　　金：国家自然科学基金 National Major Scientific and Technological Special Project for "Significant New Drugs Development" 

主　　题：al. function enzymatic 

摘      要：Dear Editor,Animal models,most commonly mice,that lack a protein of interest play an important role in phenotypic and functional studies of a target gene,allowing researchers to answer various biological questions(Chaible et al,2010).At pre-sent,a variety of tools act at the DNA or RNA level to enable researchers to model gene function(and thus protein)deficiency,including nucleic acid based RNA interference(EI-bashir et al.,2001),antisense oligonucleotides(Schoch and Miller,2017),and genome editing-based CRISPR-Cas9(Doudna and Charpentier,2014)***,challenges remain.