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Genome-wide identification and expression analysis of asparagine synthetase family in apple

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Journal of Integrative Agriculture 2020年第5期19卷 1261-1273页

作者： YUAN Xi-sen YU Zi-peng LIU Lin XU Yang ZhanG Lei han de-guo ZhanG Shi-zhong State Key Laboratory of Crop Biology Shandong Agricultural UniversityTai’an 271018P.R.China Shandong Peanut Research Institute Shandong Academy of Agricultural SciencesQingdao 266100P.R.China College of Horticulture&Landscape Architecture Northeast Agricultural UniversityHarbin 150030P.R.China

Asparagine is an efficient nitrogen transport and storage carrier. Asparagine synthesis occurs by the amination of aspartate which is catalyzed by asparagine synthetase(ASN) in plants. Complete genome-wide analysis and classifications of the ASN gene family have recently been reported in different plants. However, systematic analysis and expression profiles of these genes have not been performed in apple(Malus domestica). Here, a comprehensive bioinformatics approach was applied to identify MdASNs in apple. Then, plant phylogenetic tree, chromosome location, conserved protein motif, gene structure, and expression pattern of MdASNs were analyzed. Five members were identified and distributed on 4 chromosomes with conserved GATase-7 and ASN domains. Expression analysis indicated that all MdASNs mRNA accumulated at the highest level in reproductive organs, namely flowers or fruits, which may be associated with the redistribution of free amino acids in plant metabolic organs and reservoirs. Additionally, most of Md ASNs were dramatically up-regulated under various nitrogen supplies, especially in the aboveground part. Taken together, MdASNs may be assigned to be responsible for the nitrogen metabolism and asparagine synthesis in apple.

关键词： Malus domestica bioinformatics ASN family asparagine synthetase nitrogen metabolism

Genome-wide analysis of OVATE family proteins in cucumber(Cucumis sativus L.)

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Journal of Integrative Agriculture 2022年第5期21卷 1321-1331页

作者： han Li-jie SONG Xiao-fei WANG Zhong-yi LIU Xiao-feng YAN Li-ying han de-guo ZHOU Zhao-yang ZhanG Xiao-lan State Key Laboratories of Agrobiotechnology/Beijing Key Laboratory of Growth and developmental Regulation for Protected Vegetable Crops/MOE Joint Laboratory for International Cooperation in Crop Molecular Breeding/China Agricultural University Beijing 100193P.R.China Analysis and Testing Centre Hebei Normal University of Science&TechnologyQinhuangdao 066004P.R.China College of Horticulture Science and Technology Hebei Normal University of Science&TechnologyQinhuangdao 066004P.R.China Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of Northeast Region Ministry of Agriculture and Rural Affairs/College of Horticulture&Landscape ArchitectureNortheast Agricultural UniversityHarbin 150030P.R.China

OVATE family proteins(OFPs)are plant-specific proteins with a conserved OVATE domain that regulate plant growth and *** OFPs have been studied in several species,their biological functions remain largely unknown in cucumber(Cucumis sativus L.).This study identified 19 Cs OFPs distributed on seven chromosomes in *** Cs OFP genes were expressed in reproductive organs,but with different expression *** expression of Cs OFP12-16c in Arabidopsis resulted in shorter and blunt *** overall results indicated that Cs OFP12-16c regulates silique development in Arabidopsis and may have a similar function in cucumber.

关键词： cucumber OVATE family proteins expression pattern Cs OFP12-16c fruit development

Thermodynamic and experimental study of high-temperature roasting of blast furnace gas ash for recovery of metallic zinc and iron

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Journal of Iron and Steel Research International 2024年第1期31卷 108-120页

作者： Zi-han Wang Jing guo han-jie guo Bin Li School of Metallurgical Engineering University of Science and Technology BeijingBeijing100083China School of Metallurgical Engineering Xi’an University of Architecture and TechnologyXi’an710055ShaanxiChina

A high-temperature reduction roasting method was used to achieve metallic iron and zinc recovery from blast furnace gas ash(BFA).The reduction processes for Zn-containing and Fe-containing oxides were analyzed in detail by using ther-modynamic equilibrium calculation and the principle of minimum free *** results showed that the main reaction in the system is the reduction of ZnFe_(2)_(4)and iron *** the full temperature range,iron oxides were more easily reduced than zinc *** of the amount of CO contained in the system,the reduction of ZnO to Zn was difficult to proceed below the boiling point(906℃)of *** the reduction temperature is below 906℃,the reduction process of zinc ferrate was ZnFe_(2)_(4)→ZnO;when the reduction temperature is above 906℃,its reduction process becomed ZnFe_(2)_(4)→ZnO→Zn(g).The metallization and dezincification rates of the BFA gradually increased with increasing reaction *** the C/O ratio increased,the metallization and dezincification rates first increased and then *** effect of reduction time on BFA reduction was similar to that of reaction temperature.

关键词： Blast furnace gas ash Thermodynamics Zinc removal Iron reduction High-temperature reduction roasting Integrated utilization

A short review of antibacterial Cu-bearing stainless steel:antibacterial mechanisms,corrosion resistance,and novel preparation techniques

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Journal of Iron and Steel Research International 2024年第1期31卷 24-45页

作者： Shuai Liu han-jie guo School of Artificial Intelligence Beijing Technology and Business UniversityBeijing100048China Beijing Key Laboratory of Special Melting and Preparation of High-End Metal Materials University of Science and Technology BeijingBeijing100083China

Cu-bearing stainless steel is widely used in the fields of food,medical and household sanitary equipment because of its surface finish and corrosion ***,the growth of bacteria on stainless steel leads to the formation of biofilms,which causes ***,the antibacterial property of stainless steel is a worthy research *** of breakthroughs in the field of corrosion resistance and antimicrobial properties are still ***,due to the scarcity of publications on the antibacterial mechanisms and processing methods of antibacterial Cu-bearing stainless steel,we review the current state of relevant research and *** toxicity of Cu,corrosion resistance mechanism of stainless steel,and antibacterial mechanism and preparation method of antibacterial stainless steel are *** addition,alloying,surface modification and other methods are found to have limitations in balancing the toxicity and antibacterial properties of copper and the relationship between the antibacterial properties and corrosion resistance of Cu-bearing stainless steel.A new preparation method of antibacterial stainless steel associated with selective laser melting(SLM)is *** is becoming a powerful additive manufacturing technology that can be used to manufacture customized and complex *** research status of SLM applied in antibacterial stainless steel preparation is ***,the future research direction of Cu-bearing antibacterial stainless steel is discussed.

关键词： Cu-bearing stainless steel Antibacterial Processing Additive manufacturing Corrosion resistance

Genome-wide identification and function analysis of the sucrose phosphate synthase MdSPS gene family in apple

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Journal of Integrative Agriculture 2023年第7期22卷 2080-2093页

作者： ZhanG Li-hua ZHU Ling-cheng XU Yu LÜLong LI Xing-guo LI Wen-hui LIU Wan-da MA Feng-wang LI Ming-jun han de-guo The Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(Northeast Region) Ministry of Agriculture and Rural Affairs/National-Local Joint Engineering Research Center for Development and Utilization of Small Fruits in Cold RegionsCollege of Horticulture&Landscape ArchitectureNortheast Agricultural UniversityHarbin 150030P.R.China State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple College of HorticultureNorthwest A&F UniversityYangling 712100P.R.China Horticulture Branch Heilongjiang Academy of Agricultural SciencesHarbin 150040P.R.China

Sucrose phosphate synthase(SPS)is a rate-limiting enzyme that works in conjunction with sucrose-6-phosphate phosphatase(SPP)for sucrose synthesis,and it plays an essential role in energy provisioning during growth and development in plants as well as improving fruit ***,studies on the systematic analysis and evolutionary pattern of the SPS gene family in apple are still *** the present study,a total of seven MdSPS and four MdSPP genes were identified from the Malus domestica genome GDDH13 *** gene structures and their promoter cis-elements,protein conserved motifs,subcellular localizations,physiological functions and biochemical properties were analyzed.A chromosomal location and gene-duplication analysis demonstrated that whole-genome duplication(WGD)and segmental duplication played vital roles in MdSPS gene family *** Ka/Ks ratio of pairwise MdSPS genes indicated that the members of this family have undergone strong purifying selection during ***,three SPS gene subfamilies were classified based on phylogenetic relationships,and old gene duplications and significantly divergent evolutionary rates were observed among the SPS gene *** addition,a major gene related to sucrose accumulation(MdSPSA2.3)was identified according to the highly consistent trends in the changes of its expression in four apple varieties(‘Golden delicious’,‘Fuji’,‘Qinguan’and‘Honeycrisp’)and the correlation between gene expression and soluble sugar content during fruit ***,the virus-induced silencing of MdSPSA2.3 confirmed its function in sucrose accumulation in apple *** present study lays a theoretical foundation for better clarifying the biological functions of the MdSPS genes during apple fruit development.

关键词： apple sucrose phosphate synthase evolutionary pattern expression profile sugar accumulation

Innovations in metallurgical and materials processes:advancing liquid theory and honoring ***-jie guo's contributions to physical chemistry in metallurgy

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Journal of Iron and Steel Research International 2024年第1期31卷 1-2页

作者： Shuai Liu Jing guo han-jie guo School of Artificial Intelligence Beijing Technology and Business UniversityBeijing100048China School of Metallurgical and Ecological Engineering University of Science and Technology Beijing(USTB)Beijing100083China

Spanning three decades,***-jie guo's research group has dedicated itself to exploring the fundamental theories and practical applications of physical chemistry within the realm of *** under the assumption that molecul... 详细信息

关键词： chemistry liquid complexes

Plasma-exfoliated g-C_(3)N_(4) with oxygen doping: tailoring photocatalytic properties

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Frontiers of Chemical Science and Engineering 2024年第2期18卷 27-39页

作者： Yuxin Li Junxin guo Rui han Zhao Wang National Engineering Research Centre of Industry Crystallization Technology School of Chemical Engineering and TechnologyTianjin UniversityTianjin 300072China

Heteroatom doping and defect engineering have been proposed as effective ways to modulate the energy band structure and improve the photocatalytic activity of g-C_(3)N_(4). In this work, ultrathin defective g-C_(3)N_(4) was successfully prepared using cold plasma. Plasma exfoliation reduces the thickness of g-C_(3)N_(4) from 10 nm to 3 nm, while simultaneously introducing a large number of nitrogen defects and oxygen atoms into g-C_(3)N_(4). The amount of doped O was regulated by varying the time and power of the plasma treatment. Due to N vacancies, O atoms formed strong bonds with C atoms, resulting in O doping in g-C_(3)N_(4). The mechanism of plasma treatment involves oxygen etching and gas expansion. Photocatalytic experiments demonstrated that appropriate amount of O doping improved the photocatalytic degradation of rhodamine B compared with pure g-C_(3)N_(4). The introduction of O optimized the energy band structure and photoelectric properties of g-C_(3)N_(4). Active species trapping experiments revealed ·O_(2)^(–) as the main active species during the degradation.

关键词： graphitic carbon nitride cold plasma oxygen doping nitrogen defect visible-light photocatalysis

Enhanced corrosion resistance of epoxy resin coating via addition of CeO_(2) and benzotriazole

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Chinese Journal of Chemical Engineering 2024年第3期67卷 89-96页

作者： Xu han Ruijie guo Baolong Niu Hong Yan College of Materials Science and Engineering Taiyuan University of TechnologyTaiyuan 030024China Key Laboratory of Interface Science and Engineering in Advanced Materials Ministry of EducationTaiyuan University of TechnologyTaiyuan 030024China

The use of fillers to enhance the corrosion protection of epoxy resins has been widely *** this work,cerium dioxide(CeO_(2))and benzotriazole(BTA)were introduced into an epoxy resin to enhance the corrosion resistance of Q235 carbon *** electron microscopy results indicated that the CeO_(2) grains were rod-like and ellipsoidal in shape,and the distribution pattern of BTA was analyzed by energy dispersive *** dynamic potential polarization curve proved the excellent corrosion resistance of the composite epoxy resin with CeO_(2) and BTA co-addition,and electrochemical impedance spectroscopy test analysis indicated the significantly enhanced long-term corrosion protection performance of the composite *** the optimal protective performance was provided by the coating containing 0.3%(mass)CeO_(2) and 20%(mass)BTA,which was attributed to the barrier performance of CeO_(2) particles and the chemical barrier effect of *** formation of corrosion products was analyzed using X-ray *** addition,the corrosion resistance mechanism of the coating was also discussed in detail.

关键词： Benzotriazole Cerium dioxide Corrosion resistance Carbon steel Epoxy resin coating Preparation

Hierarchical Spinning of Janus Textiles with Anisotropic Wettability for Wound Healing

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Research 2024年第1期2023卷 97-105页

作者： han Zhang Lingyu Sun Jiahui guo Yuanjin Zhao department of Rheumatology and Immunology Nanjing Drum Tower HospitalSchool of Biological Science and Medical EngineeringSoutheast UniversityNanjingJiangsu 210096China Oujiang Laboratory(Zhejiang Lab for Regenerative Medicine Vision and Brain Health)Wenzhou InstituteUniversity of Chinese Academy of SciencesWenzhouZhejiang 325001China

Wound healing and tissue repair are recognized as basic human health problems *** to accelerate the reparative process are focused on developing functional wound ***,we present novel Janus textiles with anisotropic wettability from hierarchical microfluidic spinning for wound *** hydrophilic hydrogel microfibers from microfluidics are woven into textiles for freeze-drying treatment,followed by the deposition of electrostatic spinning nanofibers composed of hydrophobic polylactic acid(PLA)and silver *** electrospun nanofiber layer can be well coupled with the hydrogel microfiber layer to generate Janus textiles with anisotropic wettability due to the roughness of the hydrogel textile surface and the incomplete evaporation of PLA solution when reaching the *** wound treatment with the hydrophobic PLA side contacting the wound surface,the wound exudate can be pumped from the hydrophobic to the hydrophilic side based on the wettability differential derived drainage *** this process,the hydrophobic side of the Janus textile can prevent excess fluid from infiltrating the wound again,preventing excessive moisture and preserving the breathability of the *** addition,the silver nanoparticles contained in the hydrophobic nanofibers could impart the textiles with good antibacterial effect,which further promote the wound healing *** features indicate that the described Janus fiber textile has great application potential in the field of wound treatment.

关键词： silver spinning healing