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function of seminal vesicles and their role on male fertility

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Asian Journal of Andrology 2001年第4期3卷 251-258页

作者： gustavo f.gonzales Department of Physiological Sciences Faculty of Sciences and Philosophy and Instituto de Invertigaciones de la Altura Universidad Peruana Cayetano Heredia Lima Peru

The present review has been designed to update the recent developments on the function of seminal vesicles andtheir role on male fertility. It is indicated that the true corrected fructose level is a simple method for the assessment ofthe seminal vesicular function. Measurement of seminal fructose used universally as a marker of the seminal vesiclefunction is not an appropriate approach due to its inverse relationship with the sperm count. The true corrected fructosedefined as [log. motile sperm concentration] multiplied by [seminal fructose concentration] has been shown to be abetter marker of the seminal vesicle function. Seminal vesicular secretion is important for semen coagulation, sperm motility, and stability of sperm chromatinand suppression of the immune activity in the female reproductive tract. In conclusion, the function of seminal vesicle is important for fertility. Parameters as sperm motility, sperm chro-matin stability, and immuno-protection may be changed in case of its hypofunction. (Asian J Androl 2001 Dec; 3:251 -258)

关键词： seminal vesicles male fertility male infertility true corrected fructose

Effect of Lepidium meyenii(maca)roots on spermatogenesis of male rats

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Asian Journal of Andrology 2001年第3期3卷 231-233页

作者： gustavo f.gonzales Ana Ruiz Carla gonzales León Villegas Amanda Cordova Department of Physiological Sciences Faculty of Sciences and Philosophy and Instituto de Investigaciones de la Altura.Universidad Peruana Cayetano Heredia.Postal Office 1843.LimaPeru

Aim:To determine the effect of oral administration of an aqueous extract from the roots of Lepidium meyenii(maca)on spermatogenesis in adult male ***;Male rats received an aqueous extract of the root(66.7 mg in onemL)twice a day for 14 consecutive ***:Treatment with Lepidium meyenii resulted in an increase in theweights of testis and epididymis but not the seminal vesicle *** length and frequency of stages IX-XIV seminif-erous tubules,where mitosis occurred,were increased and stages I-VI were reduced in rats treated with ***;The Lepidium meyenii root invigorates spermatogenesis in male rats by acting on its initialstages(IX-XIV).

关键词： spermatogenesis Lepidium meyenii maca transillumination

Lepidium meyenii (Maca) improved semen parameters in adult men

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Asian Journal of Andrology 2001年第4期3卷 301-303页

作者： gustavo f.gonzales Amanda Cordova Carla gonzales Arturo Chung Karla Vega Arturo Villena Department of Physiological Sciences Faculty of Science and Philosopy and Instituto de Investigacions de la Altura.University Peruana Cayetano HerediaLimaPeru

Aim:The present study was designed to determine the effect of a 4-month oral treatment with tablets of Lepidiwnmeyenii(Maca)on seminal analysis in nine adult normal men aged 24-44 years ***:Nine men receivedtablets of Maca(1500 or 3000 mg/day)for 4 *** analysis was performed according to guidelines of theWorld Health Organization(WHO).Serum luteinizing hormone(LH),follicle stimulating hormone(fSH),prolactin(PRL),testosterone(T)and estradiol(E_(2))were measured before and after ***;Treatment withMaca resulted in increased seminal volume,sperm count per ejaculum,motile sperm count,and sperm *** levels were not modified with Maca *** of sperm count was not related to dose of ***:Maca improved sperm production and sperm motility by mechanisms not related to LH,fSH,PRL,Tand E_(2).

关键词： sperm count sperm motility Peruvian plants Maca Lepidium meyenii

Effect of alcoholic extract of Lepidium meyenii (Maca) on testicular function in male rats

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Asian Journal of Andrology 2003年第4期5卷 349-352页

作者： gustavo f. gonzales Julio Rubio Arturo Chung Manuel Gasco León Villegas Department of Biological and physiological Sciences faculty of Sciences and Philosophy and Instituto de Investigaciones de la Altura Department of Biochemistry Molecular Biology and Pharmacology Universidad Peruana Cayetano HerediaLima Peru

Aim: To evaluate the effect of the alcoholic extract of Lepidium meyenii (Maca) on the spermatogenesis in male rats. Methods: In Holtzman rats, Maca alcoholic extract (5%) was given by oral route at doses of 48 mg/day or 96 mg/day for 7 days, 14 days and 21 days. Testicular function was assessed by measurements of lengths of different stages of seminiferous epithelia and by epididymal sperm count. Results: Ethanolic extract of Maca increased the length of stages IX-XI of seminiferous epithelium at treatment day 7, day 14 and day 21. Progression of spermatogenesis was evident only after day 21 when lengths of stages XII-XIV of seminiferous epithelium were increased; at day 7 and day 14, no important change in spermatogenesis was observed. Epididymal sperm count was increased with 48 mg/day at all times. With 96 mg/day an increase in sperm count was observed at day 7, but it was reduced at day 14 and day 21 of treatment. Serum testosterone levels were not affected. Conclusion: The alcoholic extract of Maca activates onset ant progression of spermatogenesis at 48 mg/day or 96 mg/day in rats.

关键词： Lepidium meyenii transillumination spermatogenesis sperm count

Cycloheximide prevents production of arresting,a fraction of 30-50 kDa obtained from seminiferous tubule conditioned medium

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Asian Journal of Andrology 2004年第4期6卷 359-364页

作者： gustavo f.gonzales Stella Hartinger Yetssy flores Kelly Zárate Pedro Yi Instituto de Investigations de la Altura Department of Biological and Physiological SciencesDepartment of Biological and Physiological SciencesDepartment of Biological and Physiological SciencesFaculty of Veterinary Medicine and Animal Sciences Department of Biological and Physiological Sciences and and and and Universidad Peruana Cayetano Heredia. Postal Office 1843. Lima Peru

Aim: To evaluate the effect of a protein synthesis inhibitor cycloheximide on arresting activity in spermato-genesis and sperm count in male rats. Methods: The study used seminiferous tubule (ST) segments from adult rats cultured in vitro with or without cycloheximide to condition culture media, which have been concentrated, size fractioned (30-50 kDa) and administered 7 days to adult rats by intraperitoneal injections. The effects on testicular and epididymal weights, spermatogenesis and epididymal sperm count were determined. Results: The fraction (30-50 kDa), named arresting, obtained from the culture without cycloheximide decreased testicular and epididymal weights (Pficantly. Study of the spermatogenic cycle by transillumination showed spermatogenic arrest at stage VII in rats treated with arresting compared to that observed in controls. The length of stage VII in the group receiving the seminiferous tubules culture media with cycloheximide (30-50 KDa CHX-STCM fraction) was similar to control. Conclusion: The difference in the effect may be the result of the presence or absence of arresting, a protein secreted by the tubules.

关键词： cycloheximide arresting conditioned medium seminiferous tubule sperm production transillumination

Lepidium meyenii (Maca) reduces spermatogenic damage induced by a single dose of malathion in mice

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Asian Journal of Andrology 2005年第1期7卷 71-76,页

作者： Eduardo Bustos-Obregon Sandra Yucra gustavo f.gonzales faculty of Medicine University of Chile and lnstituto de Investigaciones de la Altura and Faculty of Sciences and Philosophy Universidad Peruana Cayetano Heredia Lima Peru

Aim: To observe the effect of the aqueous extract of hypocotyls of the plant Lepidium meyenii (Maca) on spermato-genic damage induced by the organophosphate insecticide malathion in mice. Methods: Mice were treated with 80 mg kg^(-1) of malathion in the presence or absence of an aqueous extract of Maca, which was orally administered 7, 14 or 21 days after injection of the malathion. Stages of the seminiferous epithelium were assessed by transillumination on days 0, 7, 14 and 21. Results: The administration of Maca increased significantly the length of stage 鈪?on days 7, 14 and 21 of treatment compared with the controls. An increase in the length of stage 鈪?occurred on day 14 of treatment. Malathion affected spermatogenesis by reducing the lengths of stage 鈪?on day 7, stages 鈪?and 鈪?鈪?on day 14 and a recovery of stages 鈪?鈪?on day 21. The magnitude of alteration in the length of stage 鈪?produced by malathion was significantly reduced by Maca on days 7 and 14. The length of stage 鈪?was increased when Maca was administered to mice treated with malathion. Assessment of the relative length of stages of the seminiferous epithelium showed that Maca treatment resulted in rapid recovery of the effect of malathion. Conclusion: Maca enhances spermatogenesis following spermatogenic damage caused by the organophosphorous pesticide.

关键词： malathion spermatogenesis Lepidium meyenii (Maca) mice seminiferous epithelium stages

Effect of two different extracts of red maca in male rats with testosterone-induced prostatic hyperplasia

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Asian Journal of Andrology 2007年第2期9卷 245-251页

作者： gustavo f. gonzales Vanessa Vasquez Daniella Rodriguez Carmen Maldonado Juliet Mormontoy Jimmy Portella Monica Pajuelo León Villegas Manuel Gasco Biological and Physiological Sciences Cayetano Heredia University Lima 100 Peru High Altitude Institute Research Cayetano Heredia University Lima 100 Peru faculty of Sciences and Philosophy Cayetano Heredia University Lima 100 Peru Quality Control Center Cayetano Heredia University Lima 100 Peru Department of Pharmaceutical Sciences Cayetano Heredia University Lima 100 Peru

Aim： To determine the effect of two different extracts of red maca in male rats. Methods： Prostatic hyperplasia was induced in male rats with testosterone enanthate （TE）. The study comprised six groups： one control group （group 1）, one group treated with TE （group 2）, two groups treated with TE and aqueous extract of red maca （groups 3 and 4）, one group treated with hydroalcoholic extract of red maca （group 5） and one group treated with finasteride （0.1 mg, group 6）. Differences in the aqueous extract dependent on the length of time of boiling, whether for 2 or 3 hours, for groups 3 and 4 was assessed. Extracts of red maca contained 0.1 mg of benzylglucosinolate. Thereafter, a doseresponse effect of different doses of benzylglucosinolates （0.02-0.08 mg） in red maca extracts was assessed. Results： Prostate weight was similar in rats treated with freeze-dried aqueous extract of red maca prepared after 2 and 3 hours of boiling. freeze-dried aqueous extract of red maca, hydroalcoholic extract of red maca and finasteride reduced prostate weight in rats with prostatic hyperplasia. No difference was observed between the data obtained from aqueous extract or hydroalcoholic extract of red maca. A dose dependent reduction of prostate weight was observed with the increase of the dose of benzylglucosinolates in red maca extracts. Conclusion： The present study showed that hydroalcoholic or aqueous extract of red maca containing 0.1 mg of benzylglucosinolate can reduce prostate size in male rats in which prostatic hyperplasia had been induced by TE.

关键词： red maca Lepidium meyenii freeze-dried aqueous extract hydroalcoholic extract prostatic hyperplasia prostate weight benzyl glucosinolates

Antifertility Effects of Aqueous Roots Extract of <i>Alchornea cordifolia</i>(Euphorbiaceae) on female Albino Rats

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Pharmacology & Pharmacy 2014年第8期5卷 838-845页

作者： Dieudonne Massoma Lembè Larice E. Njoh Njoh Emma f. Bend Brice L. Koloko Pierre C. Oundoum Oundoum Marie I. Ngaha Njila Hubert Kenmogne Cyprien M. Hambe Marie C. Tchamadeu Judith Domkam Theophile Dimo gustavo f. gonzales Department of Animal Biology and Physiology Faculty of Science University of Yaounde I Yaounde Cameroon Department of Animal Sciences Faculty of Science University of Douala Douala Cameroon Laboratory of Endocrinology and Reproduction Faculty of Sciences and Philosophy Alberto Cazorla Talleri Universidad Peruana Cayetano Heredia Lima Peru

Aqueous roots extract of Alchornea cordifolia was studied to evaluate its traditional antifertility claim. Antifertility effect of Alchornea cordifolia was evaluated according to the stages of pregnancy of female rats. In the first stage, forty female rats were randomly divided into four groups (10 rats/group) and respectively treated with 1 ml distilled water (control), 100, 200 and 400 mg/kg body weight (BW) per day of Alchornea cordifolia. After laparotomy and at parturition, sites of implantation and resorption on the uterine horns were determined. The ovaries and uterus were collected and weighed. Blood samples were collected for hormonal assay. In the second and third stage of pregnancy, treatment schedule and antifertility parameters were assessed as above for the first stage. Treated groups received daily 400 mg/kg bw of extract of Alchornea cordifolia while Control group received distilled water. It was observed in the first stage of pregnancy that, the number of resorption sites significantly (p fter laparotomy as well as the number of pups at parturition at all doses. The body weight of ovaries and uterine horns at dose 400 mg/kg significantly (p ficantly the concentrations of serum progesterone. In the second stage of pregnancy after laparotomy and at parturition, the number of live fetus and the survival ratio significantly (p f animals. In the third stage of pregnancy, the extract caused an early parturition associated with a decreased number of live fetus and the survival ratio with p The results indicate that aqueous roots extract of Alchornea cordifolia could induce inhibitory effects on reproductive functions in female albino rats.

关键词： Implantation Embryo Development Abortion Hormone Level

Urethane Modified Hydrophobic Compact Wood Pulp Paper for Oil Spill Cleanup: A Preliminary Study

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Journal of Renewable Materials 2020年第10期8卷 1257-1268页

作者： gustavo de Souza Ricardo K.Kramer Antonio J.f.Carvalho Department of Materials Engineering Sao Carlos School of Engineering(EESC)University of São Paulo(USP)São PauloBrazil

Oil spills and oil/water wastewater are among the great concerns regarding oil *** technologies face many limitations and in some cases are responsible for causing secondary pollution,therefore there is as seek for environmental friendly ***,from which celluloses are highlighted,are being employed for oil/water separation or oil absorbents ***,these membranes are obtained by freeze drying of CNf(cellulose nano-fibrils)suspensions followed by chemical modification for hydrophobization,which involves expensive process as chemical vapor deposition and expensive reactants as sylanes,turning these processes hardly ***,we produced a natural porous structure paper from eucalyptus pulp fibers modified by a dipping and heating process in a blocked diisocyanate *** the surface treatment,contact angle with water reached 144°and water absorption reduced seven times,keeping the good oil *** chemical modification process is simple to be performed and use a very low quantity of reactant estimated to be less than 0.1 wt%based on *** good mechanical properties of the material allows its use in non usual conditions which can be of great importance depending on the environmental conditions.

关键词： Cellulose oil removal hydrophobization water repellency blocked isocyanates