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Optimal cytokinin/auxin balance for indirect shoot organogenesis of Eucalyptus cloeziana and production of ex vitro rooted micro-cuttings

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Journal of Forestry Research 2022年第5期33卷 1573-1584页

作者： Leandro Silva de Oliveira Gilvano Ebling Brondani Letícia Vaz Molinari Rafaella Zanetti Dias gustavo leal teixeira Antônio Natal Gonçalves Marcílio de Almeida Institute of Agricultural Science Federal University of Minas GeraisUniversity Campus1000Universitária Ave.Montes ClarosMG 39404-547Brazil Laboratory of In Vitro Culture of Forest Species Department of Forestry SciencesFederal University of LavrasUniversity CampusCentral AvenueLavrasMG 37200-900Brazil Department of Biological Sciences “Luiz de Queiroz”College of AgricultureUniversity of São Paulo(ESALQ/USP)University Campus11Pádua Dias AvenuePiracicabaSP 13418-900Brazil

Shoot organogenesis is critical for the shortening of long breeding cycles and circumvent the barrier of cloning mature Eucalyptus cloeziana *** enables large-scale production of plants from transformed *** study evaluates the effect ofα-naphthaleneacetic acid(NAA),thidiazuron(TDZ)and benzylaminopurine(BAP)on the organogenesis of *** from hypocotyls and cotyledonary *** the induction stage,hypocotyls and cotyledonary leaves were established in a Murashige and Skoog(MS)culture medium supplemented with NAA or *** tissues were cultivated in a MS culture medium containing only BAP or different concentrations of BAP/NAA in the differentiation *** buds were multiplied in vitro and elongated in a WPM culture medium supplemented with 0.89μM BAP and 0.05μM *** leaves exhibited the best in vitro *** induction of adventitious buds occurred only in calluses induced from *** the differentiation stage,4.4μM BAP treatment promoted an increase of adventitious bud ***-cuttings from regenerated shoots were acclimatized and rooted ex vitro in *** results confirm the establishment of an efficient protocol for the in vitro regeneration of *** by indirect organogenesis,providing new insights regarding cloning of this species.

关键词： Micropropagation Recalcitrant species Plant growth regulator Explants source

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Femoral positioning influences ipsi-and contralateral anterior cruciate ligament rupture following its reconstruction:Systematic review and meta-analysis

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World Journal of Orthopedics 2017年第8期8卷 644-650页

作者： gustavo Constantino de Campos Paulo Eduardo Portes teixeira Alex Castro Wilson de Mello Alves Junior Department of Orthopaedics Campinas’s State University (UNICAMP) Instituto Wilson Mello Research and Study Center

AIM To systematically review the incidence of ipsilateral graft re-rupture and contralateral anterior cruciate ligament(ACL) rupture following its reconstruction, with special attention to the femoral drilling technique. METHODS Systematic review and meta-analysis of high-level prospective studies searched in MEDLINE database following PRISMA statement. The rate of ipsilateral graft re-rupture and contralateral rupture in patients submitted to either transtibial(TT) technique(isometric) or anteromedial(AM) technique(anatomic) was compared. RESULTS Eleven studies met the criteria and were included in final analysis. Reconstructions using the AM technique had a similar chance of contralateral ACL rupture when compared to the chance of ipsilateral graft failure(OR = 1.08, P = 0.746). In reconstructions using TT technique, the chance of contralateral ACL rupture was approximately 1.5 times higher than ipsilateral graft failure(OR = 1.49, P = 0.048). Incidence of contralateral lesions were similar among the techniques TT(7.4%) and AM(7.0%)(P = 0.963), but a trend could be noticed with a lower incidence of lesion in the ipsilateral limb when using the TT technique(4.9%) compared to the AM technique(6.5%)(P = 0.081).CONCLUSION ACL reconstruction by TT technique leads to lower incidence of graft re-injury than contralateral ACL lesion. There is no difference between the chance of re-injury after AM technique and the chance of contralateral ACL lesion(native ligament) with either technique.

关键词： Anterior cruciate ligament Anterior cruciate ligament reconstruction Arthroscopy Graft survival

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Biochar influences the cane fields’microbiota and the development of pre-sprouted sugarcane seedlings

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Waste Disposal and Sustainable Energy 2023年第1期5卷 75-88页

作者： Bruna Cristina Pinto Adriana Barboza Alves Osania Emerenciano Ferreira gustavo Henrique Gravatim Costa Magno Andréde Oliveira Augusto Cesar da Silva Bezerra Alan Rodrigues teixeira Machado Postgraduate Program in Environmental Sciences State University of Minas GeraisFrutal UnityFrutalMinas Gerais38202-436Brazil Department of Agricultural and Biological Sciences State University of Minas GeraisFrutal UnityFrutalMinas Gerais38208-436Brazil Postgraduate Program in Geotechnical Engineering Federal University of Ouro PretoOuro PretoMinas Gerais35400-000Brazil Department of Transport Engineering Federal Center for Technological Education of Minas GeraisBelo HorizonteMinas Gerais30421-169Brazil Department of Exact Sciences State University of Minas GeraisJoão Monlevade UnityJoão MonlevadeMinas Gerais35930-314Brazil

This research investigates the soil conditioning effect of sugar cane fields with biochar produced from sugarcane *** influence on the physicochemical and microbiological conditions of the soil and the agronomic performance of sugarcane seedlings was *** this end,the biochar from sugarcane bagasse was produced by pyrolysis in a double drum oven and mixed with soil at rates of 0,1%,3%,and 5%(in weight).The experiment was installed in a greenhouse with two evaluation periods(30 days and 60 days)and five *** pot received a pre-sprouted cane seedling,and the analysis of colony-forming units(bacteria and fungi),physicochemical characteristics of the soil,and growth and development of sugarcane were performed per *** biochar showed a high fixed carbon content(72%)and a specific surface area estimated by the adsorption of methylene blue of 50 m2 g−***,its structure is porous and contains important nutrients(e.g.,CaO,K2O,and P2O5).In the periods evaluated,the applications of the 3%and 5%(in weight)rates of biochar reduced most of the soil fertility ***,the chemical analyses indicated that the sugarcane field soil collected already possessed high *** microbiota was influenced,but only the application of the 3%(in weight)rate at 60 days after planting showed a significant positive effect on the number of bacteria forming units,with an increase of approximately 385%.On the other hand,no significant positive effect on sugarcane development was ***,biochar application in high-fertility sugarcane plantation soil did not result in gains for sugarcane development in the periods evaluated.

关键词： Soil Biometry Microbiology Double drum oven Biochar

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Morphological and kinetic parameters of the absorption of nitrogen forms for selection of Eucalyptus clones

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Journal of Forestry Research 2021年第4期32卷 1599-1611页

作者： Matheus Severo de Souza Kulmann Betania Vahl de Paula Paula Beatriz Sete Wagner Squizani Arruda Gabriel Alberto Sans Camila Peligrinotti Tarouco Luciane Almari Tabaldi Fernando teixeira Nicoloso gustavo Brunetto Soil Science Department Federal University of Santa MariaSanta MariaRS 97105-900Brazil Federal University of Santa Catarina Admar Gonzaga Highway1346FlorianópolisSC 88034-001Brazil Biology Department Natural and Exact Sciences CenterFederal University of Santa MariaSanta MariaRS 97105-900Brazil

Eucalyptus clones are selected according to productivity,wood quality,rooting capacity,and resistance to drought,frost and ***,kinetic and morphological parameters that determine the absorption efficiency of nutrients such as nitrate(NO_(3)^(-)) and ammonium(NH_(4)^(+))are often not considered in breeding *** objective of this study was to evaluate the morphological,physiological and kinetic parameters of nitrogen uptake by clones of Eucalyptus saligna(32,864) and Eucalyptus grandis(GPC23).Morphological parameters in shoot and root systems,biomass and N concentrations in different organs,photosynthetic pigment concentrations,parameters of chlorophyll a fluorescence and photosynthetic rates were *** parameters,maximum absorption velocity(V_(max)),Michaelis-Menten constant(K_(m)),minimum concentration(C_(min)) and influx(I) were calculated for NO_(3)^(-)and NH_(4)^(+) in the two *** clone was more efficient in the uptake of NO_(3)^(-)and NH_(4)^(+),and showed lower K_(m) and C_(min)values,allowing for the absorption of nitrogen at low concentrations due to the high affinity of the absorption sites of clone roots to NO_(3)^(-)and NH_(4)^(+).Higher root lengths,area and volume helped the *** clone in absorption efficiency and consequently,resulted in higher root and shoot *** *** clone had higher K_(m) and Cmin for NO_(3)^(-)and NH_(4)^(+),indicating adaptation to environments with higher N *** results of NO_(3)^(-)and NH_(4)^(+) kinetic parameters indicate that they can be used in Eucalyptus clone selection and breeding programs as they can predict the ability of clones to absorb NO_(3)^(-)and NH_(4)^(+) at different concentrations.

关键词： Ammonium and nitrate Eucalyptus saligna Eucalyptus grandis Root system architecture Nitrogen influx Maximum absorption velocity(V_(max)) Michaelis–Menten constant(K_(m)) Minimum concentration(C_(min))

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Murine model to study brain,behavior and immunity during hepatic encephalopathy

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World Journal of Hepatology 2014年第4期6卷 243-250页

作者： Lindisley Ferreira Gomides Pedro Elias Marques Bruno Engler Faleiros Rafaela Vaz Pereira Sylvia Stella Amaral Thais Reis Lage gustavo Henrique Souza Resende Patricia Alves Maia Guidine Giselle Foureaux Fabíola Mara Ribeiro Fabiana Paiva Martins Marco Antonio Peliky Fontes Anderson José Ferreira Remo Castro Russo Mauro Martins teixeira Márcio Flávio Moraes Antonio Lúcio teixeira gustavo Batista Menezes Laboratório de Imunobiofotonica Department of MorphologyICBUFMGBelo Horizonte 31270-901Brazil School of Medicine UFMGBelo Horizonte 31270-901Brazil Department of Physiology ICBUFMGBelo Horizonte 31270-901Brazil Department of Morphology UFMGBelo Horizonte 31270-901Brazil Department of Biochemistry and Immunology UFMGBelo Horizonte 31270-901Brazil

AIM:To propose an alternative model of hepatic encephalopathy(HE) in mice,resembling the human features of the ***:Mice received two consecutive intraperitoneal injections of thioacetamide(TAA) at low dosage(300 mg/kg).Liver injury was assessed by serum transaminase levels(ALT) and liver histology(hematoxylin and eosin).Neutrophil infiltration was estimated by confocal liver intravital *** was evaluated using prolonged prothrombin and partial thromboplastin *** parameters were measured through tail *** levels were quantified in serum and brain ***(EEG) and psychomotor activity score were performed to show brain *** edema was evaluated using magnetic resonance ***:Mice submitted to the TAA regime developed massive liver injury,as shown by elevation of serum ALT levels and a high degree of liver *** intense hepatic neutrophil accumulation occurred in response to TAA-induced liver *** led to mice mortality and weight loss,which was associated with severe ***,TAA-treated mice presented with increased serum and cerebral levels of ammonia,in parallel with alterations in EEG spectrum and discrete brain edema,as shown by magnetic resonance *** agreement with this,neuropsychomotor abnormalities ensued 36 h after TAA,fulfilling several HE features observed in *** this context of liver injury and neurological dysfunction,we observed lung inflammation and alterations in blood pressure and heart rate that were indicative of multiple organ dysfunction ***:In summary,we describe a new murine model of hepatic encephalopathy comprising multiple features of the disease in humans,which may provide new insights for treatment.

关键词： Hepatic encephalopathy Liver injury Thioacetamide Neurological dysfunction Neuropsychomotor abnormalities Intracranial hypertension Cerebral herniation

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Risk stratification and geographical mapping of Brazilian inflammatory bowel disease patients during the COVID-19 outbreak:Results from a nationwide survey

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World Journal of Gastroenterology 2021年第12期27卷 1226-1239页

作者： Natália Sousa Freitas Queiroz Fábio Vieira teixeira Marina Pamponet Motta Liliana Andrade Chebli Adriano Akira Ferreira Hino Camilla de Almeida Martins Abel Botelho Quaresma Alexandre Augusto de Paula da Silva Adérson Omar Mourão Cintra Damião Rogerio Saad-Hossne Paulo gustavo Kotze Department of Gastroenterology University of São Paulo School of MedicineSão Paulo 05403-000SPBrazil IBD Unit Gastroenterology DepartmentGastrosaúde ClinicMarilia 17509190SPBrazil School of Medicine Federal University of BahiaSalvador 40020-025BHBrazil IBD Outpatient Clinics Universidade Federal de Juiz de ForaJuiz de Fora 36010-010MGBrazil Health Sciences Postgraduate Program Pontifícia Universidade Católica do Paraná(PUCPR)Curitiba 80440-220PRBrazil Departamento de Ciências da Saúde Universidade do Oeste de Santa Catarina(UNOESC)Joaçaba 89600-000SCBrazil Botucatu Medical School Paulista State University(UNESP)Botucatu 18600-050SPBrazil IBD Outpatient Clinics Pontificia Universidade Católica do Paraná(PUCPR)Curitiba 80440-220PRBrazil

BACKGROUND Coronavirus disease 2019(COVID-19)pandemic is still evolving globally,and Brazil is currently one of the most affected *** is still debated whether patients with inflammatory bowel disease(IBD)are at a higher risk for developing COVID-19 or its *** To assess geographical distribution of IBD patients at the highest risk and correlate these data with COVID-19 mortality rates in *** The Brazilian IBD Study Group(Grupo de Estudos da Doença Inflamatória Intestinal do Brasil)developed a web-based survey adapted from the British Society of Gastroenterology *** included categories were demographic data and inquiries related to risk factors for complications from *** were categorized as highest,moderate or lowest individual *** Spearman correlation test was used to identify any association between highest risk and mortality rates for each state of the *** A total of 3568 patients(65.3%females)were *** participants were from the southeastern and southern regions of Brazil,and 84.1%were using immunomodulators and/or *** patients(55.1%)were at moderate risk,23.4%were at highest risk and 21.5%were at lowest risk of COVID-19 *** association between the proportion of IBD patients at highest risk for COVID-19 complications and higher mortality rates was identified in different Brazilian states(r=0.146,P=0.467).CONCLUSION This study indicates a distinct geographical distribution of IBD patients at highest risk for COVID-19 complications in different states of the country,which may reflect contrasting socioeconomic,educational and healthcare *** association between high risk of IBD and COVID-related mortality rates was identified.

关键词： Crohn’s disease Colitis,Ulcerative COVID-19 Inflammatory bowel disease Brazil

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COVID-19 liver and gastroenterology findings:An in silico analysis of SARS-CoV-2 interactions with liver molecules

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World Journal of Hepatology 2022年第6期14卷 1131-1141页

作者： Gabrielle Caroline Peiter Cristiano de Bem Torquato de Souza Lucca Miketen de Oliveira Luis gustavo Pagliarin Valentina Nunes Fontoura dos Anjos Filipe Antônio França da Silva Fabrício Freire de Melo Kádima Nayara teixeira Universidade Federal do Paraná Campus ToledoToledo 85919-899ParanáBrazil Universidade Federal da Bahia Campus Anísio TeixeiraVitória da Conquista 45029-094BahiaBrazil

BACKGROUND Coronavirus disease 19(COVID-19)has not only been shown to affect the respiratory system,but has also demonstrated variable clinical presentations including gastrointestinal tract *** addition,abnormalities in liver enzymes have been reported indicating hepatic *** is known that severe acute respiratory syndrome coronavirus-2(SARS-CoV-2)might infect cells via the viral receptor angiotensin-converting enzyme 2(ACE2)which is expressed in several organs including the *** viral Spike glycoprotein binds to ACE2 and must be cleaved by Furin and Type 2 Serine Protease to enter the *** that,the Akt/mTOR signaling pathway is activated and several COVID-19 changes are *** To analyze liver and gastrointestinal symptoms and cell signaling pathways triggered by SARS-CoV-2 infection due to virus-liver interactions in *** In this in silico study,the three-dimensional structures of the Akt,mTORC1 and Furin(receptors)were selected from the Protein Data Bank(PDB)and the structures of inhibitors(ligands)MK-2206,CC-223 and Naphthofluorescein were selected from PubChem and ZINC *** files were downloaded as 2D structures and converted to optimized 3D structures using ViewerLite 4.2 *** Sketch®software was used to calculate prediction of the protonated form of inhibitors in a physiological environment(pH 7.4).AutoDock Tools(ADT)software was used to calculate and delimit the Grid box used in the molecular docking of each structure selected in the *** addition,protonated ligands were prepared for molecular docking using ADT *** docking was performed using ADT software tools connected to Vina *** of the amino acid residues involved in ligand interactions,as well as ligand twists,the atoms involved in interactions,bond type and strength of interactions were performed using PyMol^(■)and Discovery Studio^(■)(BIOVIA)*** Molecular docking analysis showed that the mTOR

关键词： Bioinformatics Cell signaling pathway COVID-19 Liver injury SARS-CoV-2

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Antitumor effects of electrochemical treatment

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Chinese Journal of Cancer Research 2013年第2期25卷 223-234页

作者： Héctor Manuel Camué Ciria Maraelys Morales González Lisset Ortíz Zamora Luis Enrique Bergues Cabrales gustavo Victoriano Sierra González Luciana Oliveira de Oliveira Rodrigo Zanella Antonio Carlos Buzaid Orlando Parise Luciana Macedo Brito Cesar Augusto Antunes teixeira Marina das Neves Gomes Gleyce Moreno Venicio Feo da Veiga Marcos Telló Carla Holandino Research Department National Centre of Applied Electromagnetism Oriente University Pharmacy Department Oriente University Finlay Institute La lisa. La Habana Cuba Veterinary Teaching Hospital Federal University of Rio Grande do Sul. Porto Alegre Sírio e Libanês Hospital Faculty of Pharmacy Medicine Department Federal University of Rio de Janeiro Professor Paulo de Góes Institute of Microbiology Federal University of Rio de Janeiro Pontifical Catholic University of Rio Grande do Sul. Porto Alegre

Electrochemical treatment is an alternative modality for tumor treatment based on the application of a low intensity direct electric current to the tumor tissue through two or more platinum electrodes placed within the tumor zone or in the surrounding areas. This treatment is noted for its great effectiveness, minimal invasiveness and local effect. Several studies have been conducted worldwide to evaluate the anfitumoral effect of this therapy. In all these studies a variety of biochemical and physiological responses of tumors to the applied treatment have been obtained. By this reason, researchers have suggested various mechanisms to explain how direct electric current destroys tumor ceils. Although, it is generally accepted this treatment induces electrolysis, electroosmosis and electroporation in tumoral tissues. However, action mechanism of this alternative modality on the tumor tissue is not well understood. Although the principle of Electrochemical treatment is simple, a standardized method is not yet available. The mechanism by which Electrochemical treatment affects tumor growth and survival may represent more complex process. The present work analyzes the latest and most important research done on the electrochemical treatment of tumors. We conclude with our point of view about the destruction mechanism features of this alternative therapy. Also, we suggest some mechanisms and strategies from the thermodynamic point of view for this therapy. In the area of Electrochemical treatment of cancer this tool has been exploited very little and much work remains to be done. Electrochemical treatment constitutes a good therapeutic option for patients that have failed the conventional oncology methods.

关键词： Direct electric current electrochemical treatment antimmoral treatment tumor tumor electrolysis rumor entropy dissipative structure

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Short-Time Derivatization Method for Analysis of Abamectin in Water Using High-Performance Liquid Chromatography Coupled to Fluorescence Detector

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American Journal of Analytical Chemistry 2014年第16期5卷 1102-1108页

作者： Daniela Cordeiro Luciana Teresa Dias Cappelini Raphael teixeira Verbinnen gustavo Henrique Lourenco Vicente Andréa Novelli Evaldo Luiz Gaeta Espíndola Eny Maria Vieira Chemistry Institute of Sao Carlos Sao Paulo UniversitySao CarlosBrazil Center for Studies in Aquatic Ecosystems Sao Paulo UniversitySao CarlosBrazil

A method using a short-time derivatization step for the assessment of abamectin in water is presented. Abamectin derivative stable up to 7 days was obtained. Some regions where orange crops are present have received abamectin doses, aiming to increase the productivity and to combat pests and weeds, even when its residues reach the aquatic environment and interfere on water quality. Water samples from Jacaré-Pepira River (Brotas City, Brazil) nearby orange crops around urban zone, were evaluated for the presence of abamectin. The analytical method was validated resulting recovery around 108%, precision of 12%, accuracy of 104%, correlation coefficient of 0.9945, and detection and quantification limits of 0.1 μg·L﹣1 and 0.2 μg·L﹣1, respectively. Stable abamectin derivative was reached after 60 min of derivatization at room temperature (25°C). No abamectin residues were found into samples.

关键词： Abamectin Derivatization SPE HPLC-FLD Water

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Macrolide-Clarithromycin Task-Force for the Treatment and Prophylaxis of Covid-19 as a Single Agent

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Pharmacology & Pharmacy 2020年第6期11卷 85-104页

作者： Eduardo Mansilla Ricardo Rangel Martínez gustavo Horacio Marín Idiberto Zotarelli Filho Elsa Rivas Jaime Rivas Katherine Athayde teixeira de Carvalho Mohammad Reza Dayer Alí Samadikuchaksaraei Internal Medicine School of Medical Sciences UNLP La Plata Argentina ExomePharma Ciudad de México DF México Pharmacology School of Medical Sciences UNLP La Plata Argentina Faceres Medical School Sao José do Rio Preto Sao Paulo Brazil Intensive Care Units-Hospital de la Amistad Perú Corea II Santa Rosa and Salvador University Hospital Piura Perú Intensive Care Unit Hospital de la Amistad Perú Corea II Santa Rosa Piura Perú Pequeno Príncipe Faculty & Pelé Pequeno Príncipe Institute Curitiba Paraná Brazil Department of Biology Faculty of Science Shahid Chamran University of Ahvaz Ahvaz Iran Department of Tissue Engineering & Regenerative Medicine Iran University of Medical Sciences Tehran Iran

SARS-CoV-2 is a novel RNA coronavirus responsible of a deadly pandemic: the clinical illness COVID-19. With only one authorized drug for emergency use in critically ill patients: Remdesivir, there is not any other approved drug or vaccine yet with proven potential to overcome this infection. We exposed here many scientific evidences to support our novel idea that a macrolide, basically Clarithromycin, could be effective as a single agent for treatment and prophylaxis of COVID-19. Clarithromycin could change the history of this pandemic. It could reduce the costs of treatment and the potential adverse effects when combining more than one drug such as with Hydroxychloroquine. Clarithromycin treatment and prophylaxis as a single agent could be much more simple, safe and cheaper as giving Chloroquine or Hydroxychloroquine alone or in combination with Azithromycin as well as other therapeutic options.

关键词： COVID-19 Macrolides Clarithromycin Single Agent Therapy Prophylaxis

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