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Transcriptome Analysis of Inflorescence Development at the Five-Leaf Stage in Castor(Ricinus communis L.)

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Phyton-International Journal of Experimental Botany 2024年第4期93卷 713-723页

作者： Yong Zhao Yaxuan Jiang li Wen Rui Luo guorui li Jianjun Di Mingda Yin Zhiyan Wang Fenglan Huang Fanjuan Meng College of life Science Baicheng Normal UniversityBaicheng137000China College of life Science and Food Inner Mongolia University for the NationalitiesTongliao028000China College of life Science Northeast Forestry UniversityHarbin150040China Key Laboratory of Castor Breeding of the State Ethnic Affairs Commission Inner Mongolia Minzu UniversityTongliao028043China Inner Mongolia Industrial Engineering Research Center of Universities for Castor Inner Mongolia Minzu UniversityTongliao028043China Inner Mongolia Key Laboratory of Castor Breeding and Comprehensive Utilization Inner Mongolia Minzu UniversityTongliao028000China Inner Mongolia Engineering Research Center of Industrial Technology Innovation of Castor Inner Mongolia Minzu UniversityTongliao028000China

The yield of castor is influenced by the type of inflorescence and the proportion of female flowers.However,there are few studies on the genetic mechanism involved in the development and differentiation of castor inflorescences.In this study,we performed transcriptomic analyses of three different phenotypes of inflorescences at the five-leaf stage.In comparison to the MI(complete pistil without willow leaves),290 and 89 differentially expressed genes(DEGs)were found in the SFI(complete pistil with willow leaves)and the BI(monoecious inflorescence),respectively.Among the DEGs,104 and 88 were upregulated in the SFI and BI,respectively,compared to the MI.In addition,186 DEGs and 1 DEG were downregulated in the SFI and BI compared to the MI.Moreover,we conducted GO and KEGG enrichment analyses of the DEGs.In comparison to the MI,the SFI and BI exhibited the enrichment of functional branches in DEGs,specifically in pollen wall assembly,pollen development,and cellular component assembly involved in morphogenesis.In our study,RADL5 showed low expression levels between SFI-vs.-MI types.In addition,we found that the expression of NAC in the SFI differed from that in MI and BI,and some genes related to hormonal signaling changed their expression levels during inflorescence differentiation.These results reveal the genetic mechanism of sex genotypes in castor,which will not only guide researchers in the breeding of castor but also provide a reference for genetic research on other flowering plants.

关键词： Ricinus communis L RNA-Seq inflorescence

Synthesis and assembly of full-length cyanophage A-4L genome

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Synthetic and Systems Biotechnology 2023年第1期8卷 121-128页

作者： Ting Zhang Bonan Xu Jia Feng Pingbo Ge guorui li Jiabao Zhang Jianting Zhou Jianlan Jiang School of Chemical Engineering and Technology Tianjin UniversityTianjin300072China Key Laboratory of Systems Bioengineering(Ministry of Education) Tianjin UniversityTianjin300072China Frontier Science Center for Synthetic Biology(Ministry of Education) Tianjin UniversityTianjin300072China

Artificial cyanophages are considered to be an effective biological method to control harmful cyanobacterial bloom.However,no synthetic cyanophage genome has been constructed and where its obstacles are unclear.Here,we survey a stretch of 16 kb length sequence of cyanophage A-4L that is unclonable in Escherichia coli.We test 12 predicted promoters of cyanophage A-4L which were verified all active in E.coli.Next,we screen for eight ORFs that hindered the assembly of intermediate DNA fragments in E.coli and describe that seven ORFs in the 16 kb sequence could not be separately cloned in E.coli.All of unclonable ORFs in high-copy-number plasmid were successfully cloned using low-copy-number vector,suggesting that these ORFs were copy-number-dependent.We propose a clone strategy abandoned the promotor and the start codon that could be applied for unclonable ORFs.Last,we de novo synthesized and assembled the full-length genome of cyanophage A-4L.This work deepens the understanding of synthetic cyanophages studies.

关键词： Genome assembly Cyanophage A-4L Toxic ORFs screening

A Reactive Oxygen Species-Tyrosinase Cascade-Activated Prodrug for Selectively Suppressing Melanoma

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CCS Chemistry 2022年第5期4卷 1654-1670页

作者： guorui li Yi Yang Yaya Zhang Peiling Huang Jiangyu Yan Zhibin Song Quan Yuan Jing Huang Institute of Chemical Biology and Nanomedicine(ICBN) State Key Laboratory of Chemo/Biosensing and ChemometricsCollege of BiologyCollege of Chemistry and Chemical EngineeringHunan Provincial Key Laboratory of Biomacromolecular Chemical BiologyHunan UniversityChangsha 410082 Key Laboratory of Functional Small Organic Molecules Ministry of EducationCollege of Chemistry and Chemical EngineeringJiangxi Normal UniversityNanchang 330022

“Off-target effect”is one of the obstacles for targeted prodrugs in chemotherapy.To circumvent this issue,herein we propose a dual biomarker cascadeactivated prodrug strategy based on high levels of reactive oxygen species(ROS)and tyrosinase(TYR)in melanoma cells.A representative prodrug,Coumarin-Quinazolinone-phenylBoronic acid pinacol ester(CQB),was prepared.The prodrug contained Coumarin-Quinazolinone(CQ)as mitochondria targeting and monitoring moiety and phenylboronic acid pinacol ester as a cascade-activated prowarhead.After activation by the endogenous ROS and subsequent TYR in melanoma cells,CQB can be converted to the final active reagent Coumarin-Quinazolinone-o-Quinone(CQQ)that contains o-quinone group as the reactive species.CQQ may interact with cellular nucleophiles to exert its genotoxic effect and disrupt the cellular redox balance.This enables CQB to selectively suppress melanoma.CQB accumulates in the mitochondria and causes mitochondrial dysfunction via affecting mitochondrial DNA integrity.This cascade-activated prodrug may provide a precise strategy for melanoma theranostics.

关键词： prodrug cascade activated ROS TYR melanoma mitochondria redox homeostasis theranostics

An Energy-Efficient Data Collection Scheme Using Denoising Autoencoder in Wireless Sensor Networks

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Tsinghua Science and Technology 2019年第1期24卷 86-96页

作者： guorui li Sancheng Peng Cong Wang Jianwei Niu Ying Yuan School of Computer Science and Engineering Northeastern University School of Information Science and Technology Guangdong University of Foreign Studies State Key Laboratory of Virtual Reality Technology and Systems Beihang University

As one of the key operations in Wireless Sensor Networks(WSNs), the energy-efficient data collection schemes have been actively explored in the literature. However, the transform basis for sparsifing the sensed data is usually chosen empirically, and the transformed results are not always the sparsest. In this paper, we propose a Data Collection scheme based on Denoising Autoencoder(DCDA) to solve the above problem. In the data training phase, a Denoising AutoEncoder(DAE) is trained to compute the data measurement matrix and the data reconstruction matrix using the historical sensed data. Then, in the data collection phase, the sensed data of whole network are collected along a data collection tree. The data measurement matrix is utilized to compress the sensed data in each sensor node, and the data reconstruction matrix is utilized to reconstruct the original data in the sink.Finally, the data communication performance and data reconstruction performance of the proposed scheme are evaluated and compared with those of existing schemes using real-world sensed data. The experimental results show that compared to its counterparts, the proposed scheme results in a higher data compression rate, lower energy consumption, more accurate data reconstruction, and faster data reconstruction speed.

关键词： wireless sensor networks data collection neural networks autoencoder data reconstruction

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Electromechanical behavior of fiber-reinforced dielectric elastomer membrane

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International Journal of Smart and Nano Materials 2015年第2期6卷 124-134页

作者： Chi li Yuhan Xie guorui li Xuxu Yang Yongbin Jin Tiefeng li Institute of Applied Mechanics Zhejiang University38 Zheda RoadHangzhou 310027China

Based on its large deformation,light weight,and high energy density,dielectric elastomer(DE)has been used as driven muscle in many areas.We design the fiberreinforced DE membrane by adding fibers in the membrane.The deformation and driven force direction of the membrane can be tuned by changing the fiber arrangements.The actuation in the perpendicular direction of the DE membrane with long fibers first increases and then decreases by the increasing of the fiber spacing in the perpendicular direction.The horizontal actuation of the membrane decreases by decreasing the spacing of short fibers.In the membrane-inflating structure,the radially arranged fibers will break the axisymmetric behavior of the structure.The top area of the inflated balloon without fiber will buckle up when the voltage reaches a certain level.Finite element simulations based on nonlinear field theory are conducted to investigate the effects of fiber arrangement and verify the experimental results.This work can guide the design of fiber-reinforced DE.

关键词： Dielectric elastomer fiber reinforced electromechanical

Ion channel mechanism of gastrointestinal motility and gastric hypersensitivity in functional dyspepsia:a review

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Gastroenterology & Hepatology Research 2022年第3期4卷 23-29页

作者： Terigele Bao Feng Lan guorui li Xiyele Mu Ta Na Minghai Fu Yongsheng Chen School of Mongolian Medicine Inner Mongolia University for NationalitiesTongliao 028000Inner Mongolia Autonomous RegionChina School of life Sciences Inner Mongolia University for NationalitiesTongliao 028000Inner Mongolia Autonomous RegionChina

Functional dyspepsia(FD)is a regularly diagnosed clinical gastrointestinal ailment with a high incidence rate that can considerably impact patients’health and quality of life and impose a substantial financial burden.Modern research on the pathophysiology of functional dyspepsia has not thoroughly explained the underlying reasons.The condition does not manifest any significant organ abnormalities,which raises the disease’s difficulty coefficient.Major pathogenic exceptions in FD include gastrointestinal motor dysfunction,gastrointestinal hormone secretion problem,visceral hypersensitivity,and brain-gut axis.Several ion channels have reportedly been implicated in the pathophysiological process of FD.Therefore,it is crucial to comprehend the probable activities of various ion channels in FD.This study focuses on the current state of research on the possible role of several ion channels in the pathogenesis of FD.

关键词： functional dyspepsia ion channel gastrointestinal motility gastric hypersensitivity smooth muscle

Electro-mechanically controlled assembly of reconfigurable 3D mesostructures and electronic devices based on dielectric elastomer platforms

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National Science Review 2020年第2期7卷 342-354页

作者： Wenbo Pang Xu Cheng Haojie Zhao Xiaogang Guo Ziyao Ji guorui li Yiming liang Zhaoguo Xue Honglie Song Fan Zhang Zheng Xu Lei Sang Wen Huang Tiefeng li Yihui Zhang Applied Mechanics Laboratory Department of Engineering Mechanics Tsinghua University Center for Flexible Electronics Technology Tsinghua University School of Microelectronics Soft Membrane Electronic Technology Laboratory Hefei University of Technology Zhejiang Lab State Key Laboratory for Manufacturing and Systems Engineering School of Mechanical Engineering Xi'an Jiaotong University Center for X-Mechanics Zhejiang University

The manufacture of 3D mesostructures is receiving rapidly increasing attention, because of the fundamental significance and practical applications across wide-ranging areas. The recently developed approach of buckling-guided assembly allows deterministic formation of complex 3D mesostructures in a broad set of functional materials, with feature sizes spanning nanoscale to centimeter-scale. Previous studies mostly exploited mechanically controlled assembly platforms using elastomer substrates, which limits the capabilities to achieve on-demand local assembly, and to reshape assembled mesostructures into distinct 3D configurations. This work introduces a set of design concepts and assembly strategies to utilize dielectric elastomer actuators as powerful platforms for the electro-mechanically controlled 3D assembly. Capabilities of sequential, local loading with desired strain distributions allow access to precisely tailored 3D mesostructures that can be reshaped into distinct geometries, as demonstrated by experimental and theoretical studies of30 examples. A reconfigurable inductive–capacitive radio-frequency circuit consisting of morphable 3D capacitors serves as an application example.

关键词： 3D assembly buckling reconfigurable structures dielectric elastomers reconfigurable RF circuits

褪黑素对链脲佐菌素诱导的糖尿病肺纤维化的作用及机制研究

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中华内分泌代谢杂志 2024年第6期40卷 505-514页

作者：付庭吕李国瑞熊锐何如愿刘博昊李宁耿庆武汉大学人民医院胸外科武汉吉林大学第一医院胸外科长春

目的探讨褪黑素对链脲佐菌素(streptozotocin, STZ)诱导的糖尿病肺纤维化的作用及其具体调控机制。方法将C57BL/6小鼠以随机数字表法随机分为对照组、STZ组、STZ+低剂量褪黑素(5 mg/kg)组、STZ+高剂量褪黑素(30 mg/kg)组, 采用单次腹腔... 详细信息

目的探讨褪黑素对链脲佐菌素(streptozotocin, STZ)诱导的糖尿病肺纤维化的作用及其具体调控机制。方法将C57BL/6小鼠以随机数字表法随机分为对照组、STZ组、STZ+低剂量褪黑素(5 mg/kg)组、STZ+高剂量褪黑素(30 mg/kg)组, 采用单次腹腔注射STZ(150 mg/kg)建立糖尿病肺纤维化小鼠模型, 2周后血糖≥16.7 mmol/L为造模成功, 随后给予褪黑素灌胃4周并喂养至16周处死取材。将人脐静脉内皮细胞分为对照组(葡萄糖浓度为5.5 mmol/L)、高糖组(葡萄糖浓度为33.3 mmol/L)、高糖+低剂量褪黑素(5 μmol/L)组、高糖+高剂量褪黑素(20 μmol/L)组, 药物刺激结束后收集各组细胞用于后续检测。采用马松染色和免疫荧光染色观察肺纤维化病变, Western印迹法检测相关蛋白表达, 采用转染沉默调节蛋白3(Sirtuin 3, Sirt3)siRNA的方法敲低Sirt3。结果与对照组相比, STZ组肺组织发生显著的纤维化病变, 而STZ+低剂量褪黑素组、STZ+高剂量褪黑素组纤维化均较STZ组减少。同时, 与对照组相比, STZ/高糖组内皮细胞标志物血小板-内皮细胞黏附分子(CD31)显著减少(P<0.001;P<0.001), 间质纤维化标志物胶原蛋白3(Collagen 3)、波形蛋白(Vimentin)和α-平滑肌肌动蛋白(α-SMA)显著增加(P<0.001,P=0.035,P<0.001;P<0.001,P<0.001,P<0.001), 而STZ/高糖+低剂量褪黑素组、STZ/高糖+高剂量褪黑素组在给予褪黑素治疗后上述趋势被部分逆转。此外, 与对照组相比, STZ/高糖组Sirt3的蛋白表达显著减少(P<0.001;P<0.001), 而STZ/高糖+低剂量褪黑素组、STZ/高糖+高剂量褪黑素组的Sirt3的蛋白表达较STZ/高糖组增加(P=0.047,P<0.001;P=0.048,P<0.001)。转染Sirt3 siRNA敲低Sirt3的表达后, 与高糖+高剂量褪黑素组相比, 高糖+高剂量褪黑素+Sirt3 siRNA组内皮细胞标志物CD31显著减少(P=0.026), 间质纤维化标志物Collagen 3、Vimentin和α-SMA显著增加(P<0.001,P<0.001,P<0.001)。结论褪黑素通过激活Sirt3的表达抑制内皮间质转化, 进而减轻STZ诱导的糖尿病小鼠肺纤维化。

关键词：糖尿病肺纤维化褪黑素链脲佐菌素 Sirt3 内皮间质转化

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小型深海自主定高模块动力学建模与减摇设计

中国科学：物理学、力学、天文学 2024年第6期54卷 125-135页

作者：王神龙侯锦辉俞代卫沈鹏张志函李海李国瑞上海理工大学机械工程学院上海200093 青岛哈尔滨工程大学创新发展中心青岛266071 哈尔滨工程大学船舶工程学院哈尔滨150001 杭州电子科技大学电子信息学院杭州310018

深海智能装备在深海科考、国防安全等领域发挥重要作用,受深海极端静水压影响,其水下操控和姿态控制面临巨大挑战.本文设计了一种适用于3000 m水深的自主定高模块,该模块通过将电路板集成在硅胶基质中实现深海极端水压自适应,并采用电... 详细信息

深海智能装备在深海科考、国防安全等领域发挥重要作用,受深海极端静水压影响,其水下操控和姿态控制面临巨大挑战.本文设计了一种适用于3000 m水深的自主定高模块,该模块通过将电路板集成在硅胶基质中实现深海极端水压自适应,并采用电磁线圈驱动PET背鳍薄片实现动力定高.本文利用高速相机分析了该模块下潜运动过程中的横摇现象,并进行运动学、动力学建模分析.此外,本文通过胸鳍形状的合理设计,减少模块横摇并优化其机动性.最后,通过高压实验验证了定高设计方案的可行性.该定高模块体积小、轻质、集成度高,易于实现深海装备和机器人集成,可为小型化深海机器人定高和姿态控制提供新方法.

关键词：深海定高横摇分析动力学建模减摇设计

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