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Immunomodulatory effects of Caulerpa racemosa var peltata polysaccharide and its selenizing product on T lymphocytes and NK cells in mice

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Science China(Life Sciences) 2008年第9期51卷 795-801页

作者： SHEN WeiZai WANG Hui guo guoqing TUO JingJing Department of Anatomy Medical CollegeJinan UniversityGuangzhou 510630China Ultrasonic Division Municipal Hospital of WeihaiShandong 264200China

A polysaccharide, CrvpPS, was isolated from Caulerpa racemosa var peltata. It was reacted with nano-selenium in distilled water containing ascorbic acid (Vit C) to form a stable CrvpPS-nano-Se complex. The immunomodulatory effects of CrvpPS and CrvpPS-nano-Se on T lymphocytes subgroups and NK cells in mice were investigated. After intragastric administration for 10 days separately, both CrvpPS and CrvpPS-nano-Se showed significant stimulatory functions to thymus gland of mice. Moreover, the CrvpPS-nano-Se induced the percentage of CD3+, CD3+CD4+, NK cells and the CD4+/CD8+ value to increase significantly (P<0.05) when analyzed by flow cytometry, which is better than the CrvpPS, sucrose-nano-Se, and even the positive drug levamisole.

关键词： Caulerpa racemosa var peltata polysaccharides(CrvpPS) nano-Se T lymphocyte subgroups natural killer cells immunomodulation flow cytometric analysis

Rho-associated protein kinase modulates neurite extension by regulating microtubule remodeling and vinculin distribution

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Neural Regeneration Research 2013年第32期8卷 3027-3035页

作者： Ke'en Chen Wenbin Zhang Jing Chen Sumei Li guoqing guo Department of Neurosurgery First Affiliated Hospital of Jinan University Department of Emergency First Affiliated Hospital of Jinan University Department of Anatomy Medical College of Jinan University

Rho-associated protein kinase is an essential regulator of cytoskeletal dynamics during the process of neurite extension. However, whether Rho kinase regulates microtubule remodeling or the distri- bution of adhesive proteins to mediate neurite outgrowth remains unclear. By specifically modulat- ing Rho kinase activity with pharmacological agents, we studied the morpho-dynamics of neurite outgrowth. We found that lysophosphatidic acid, an activator of Rho kinase, inhibited neurite out- growth, which could be reversed by Y-27632, an inhibitor of Rho kinase. Meanwhile, reorganization of microtubules was noticed during these processes, as indicated by their significant changes in the soma and growth cone. In addition, exposure to lysophosphatidic acid led to a decreased mem- brane distribution of vinculin, a focal adhesion protein in neurons, whereas Y-27632 recruited vin- culin to the membrane. Taken together, our data suggest that Rho kinase regulates rat hippocampal neurite growth and microtubule formation via a mechanism associated with the redistribution of vinculin.

关键词： neural regeneration brain injury Rho-associated protein kinase neurite outgrowth microtubule remodeling vinculin neuron hippocampus lysophosphatidic acid Y-27632 grants-supportedpaper neuroregeneration

Effect of Rho-kinase pathway on neurite outgrowth of rat hippocampal neurons under atomic force microscopy

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Neural Regeneration Research 2012年第7期7卷 496-500页

作者： Jing Chen Hu Hao guoqing guo Sitao Li Xin Xiao Institute of Gastroenteroloav. Sixth A ffifiated Hospital of Sun Yat-sen University Guangzhou 510655 Guangdong Province China Laboratory of Inborn Errorsof Metabolism Sixth Affifiated Hospital of Sun Yat-sen University Guangzhou 510655 Guangdong ProvinceChina Department of Anatomy Medical College of Jinan University Guangzhou 510632 Guangdong Province China

Hippocampal neurons of neonatal rats were cultured in serum-free culture medium for 5 days in vitro, and treated with the Rho-kinase inducer lysophosphatidic acid. Atomic force microscopy revealed that the numbers of level-1, -2 and -3 neurites protruding from rat hippocampal neurons was significantly reduced. After treatment with the Rho kinase inhibitor Y27632, a significant increase in the numbers of these neurites was observed. Our experimental findings indicate that the Rho-kinase pathway is closely associated with the neurites of hippocampal neurons.

关键词： atomic force microscopy Rho-kinase nerve cells neurites hippocampus rats neural regeneration

Antibody to collapsin response mediator protein 1 promotes neurite outgrowth from rat hippocampal neurons

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Neural Regeneration Research 2011年第20期6卷 1537-1542页

作者： Hongsheng Lin Jing Chen Wenbin Zhang Xiaobing Gong Biao Chen guoqing guo Department of Orthopedics the First Affiliated Hospital of Jinan University Guangzhou 510630 Guangdong Province China Department of Anatomy Medical College of Jinan University Guangzhou 510630 Guangdong Province China

This study examined the role of collapsin response mediator protein 1 （CRMP-1） on neurite outgrowth from rat hippocampal neurons by blocking its function using an antibody. Hippocampal neurons, cultured in vitro, were treated （blocked） using a polyclonal antibody to CRMP-1, and neurite outgrowth and cytoskeletal changes were captured using atomic force microscopy and laser confocal microscopy. Control cells, treated with normal rabbit IgG, established their characteristic morphology and had a large number of processes emerging from the soma, including numerous branches. Microtubules were clearly visible in the soma, formed an elaborate network, and were aligned in parallel arrays to form bundles which projected into neurites. After blocking with CRMP-1 antibody, the number of branches emerging from axons and dendrites significantly increased and were substantially longer, compared with control cells. However, the microtubule network nearly disappeared and only a few remnants were visible. When CRMP-1 antibody-blocked neurons were treated with the Rho inhibitor, Y27632, numerous neurites emerged from the soma, and branches were more abundant than in control neurons. Although the microtubules were not as clearly visible compared with neurons cultured in control medium, the microtubule network recovered in cells treated with Y27632, when compared with cells that were blocked by CRMP-1 antibody （but not treated with Y27632）. These results demonstrate that neurite outgrowth from hippocampal neurons can be promoted by blocking CRMP-1 with a polyclonal antibody.

关键词： collapsin response mediator protein 1 neuron microtubule neurite antibody block hippocampus rat neural regeneration

mRNA expression of Rho GTPase-related signaling molecules during rat hippocampal development

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Neural Regeneration Research 2009年第10期4卷 786-790页

作者： guoqing guo Jifeng Zhang Li Xin Jing Chen Weizai Shen Lin Yuan Shizhen Zhong Department of Anatomy Medical College of Jinan University Guangzhou 510630 Guangdong Province China Institute of Clinical Anatomy Southern Medical University Guangdong Key Laboratory of Tissue Construction and Detection 510150 Guangdong Province China

BACKGROUND： Rho GTPase family members have been shown to participate in neurite growth by regulating the neuronal cytoskeleton. However, there are very few reports of developmental roles of signaling molecules related to Rho GTPases. OBJECTIVE： To investigate messenger ribonucleic acid mRNA expression of signaling molecules associated with Rho GTPases, including Rho-A, Rac-1, collapsin response mediator protein 1 （CRMP-1）, and tubulin 133 （Tub/33） during rat hippocampus development. DESIGN, TIME AND SETTING- A non-randomized, controlled, animal experiment, based on different developmental stages of the rat hippocampus, was performed at the Guangdong Key Laboratory of Tissue Construction and Detection, Institute of Clinical Anatomy, Southern Medical University between December 2005 and July 2007. MATERIALS： Trizol reagent was purchased from Invitrogen, USA. RNA PCR kit （AMV） Ver 3.0 and 150 bp DNA Ladder Marker were purchased from TaKaRa, Japan. Unless otherwise specified, all other reagents were purchased from Sigma-Aldrich, USA. METHODS： Twenty-five Sprague Dawley rats were assigned to five groups （n = 5） according to developmental stages： embryonic （embryonic 15 days）, neonatal （postnatal 5 days）, juvenile （postnatal 1 month）, adult （postnatal 3 months）, and senile （postnatal 18 months）. MAIN OUTCOME MEASURES： Detection of mRNA expression of Rho-A, Rac-1, CRMP-1, and Tub β3 during various hippocampal developmental stages by reverse-transcription polymerase chain reaction. RESULTS： Hippocampal mRNA expression of Rho-A, as well as Rac-1, reached peak levels at embryonic, juvenile, and senile stages, and was relatively less during neonatal and adult stages. mRNA expression of Rac-1 was greater than Rho-A during each hippocampal developmental stage. CRMP-1 mRNA expression levels were as follows： embryonic 〉 neonatal 〉 juvenile 〉 adult 〈 senile, while Tubβ3 mRNA expression was embryonic 〉 neonatal 〉 juvenile 〉 adult = senile. CONCLUSION： Rho-A and Rac-1 shared similar

关键词： Rho GTPases collapsin response mediator protein 1 tubulin hippocampus development rat

Surgical boot camp curriculum improves clinical competencies in senior medical students

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解剖学杂志 2021年第S1期44卷 250-251页

作者： Zhang Jifeng guo guoqing Department of Anatomy Medical College of Jinan UniversityGuangzhou 510632

To provide a clinical practice skills training curriculum for senior medical students,and to assess its effect on the students'clinical practice skills and operative *** boot camp program was held by Jinan *** 40-hour surgical boot camp program composed of lectures on clinical issues,clinical practice simulation,field anatomy,and simulated operation based on fresh *** participants were 24 undergraduates of 4th grade in medical school will enter hospitals to be preliminary *** results showed that 95.0%of students were satisfied with the surgical boot camp,and for the content setting,92.2%of students thought it was appropriate for them,and the training curriculum gave them a valuable clinical practice experience.

关键词： anatomy senior curriculum

Surgical boot camp curriculum improves clinical competencies in senior medical students

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Surgical boot camp curriculum improves clinical competencies...

中国解剖学会2021年年会

作者： Zhang Jifeng guo guoqing Department of Anatomy Medical College of Jinan University

To provide a clinical practice skills training curriculum for senior medical students, and to assess its effect on the students’clinical practice skills and operative *** boot camp program was held by Jinan *** 40-hour surgical boot camp program composed of lectures on clinical issues,clinical practice simulation,field anatomy,and simulated operation based on fresh cadavers.

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来源： cnki会议评论

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编辑审稿时如何利用参考文献进行量化评价

中国科技期刊研究 2006年第6期17卷 1189-1191页

作者：郭国庆 <河南科技大学学报>(自然科学版)编辑部洛阳市西苑路48号471003

参考文献是编辑评审论文时重点审查的一项内容,利用文献计量学的一些研究成果,从参考文献角度,对科技论文的参考文献数量、类型、出版年限、语种等进行了分析和量化评价,为编辑人员初审和修改论文提供一可操作方法,以提高论文质量和期... 详细信息

参考文献是编辑评审论文时重点审查的一项内容,利用文献计量学的一些研究成果,从参考文献角度,对科技论文的参考文献数量、类型、出版年限、语种等进行了分析和量化评价,为编辑人员初审和修改论文提供一可操作方法,以提高论文质量和期刊质量。

关键词：参考文献科技文献文献计量学半衰期利用率编辑评价

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马国翰藏书种类与数量考

图书情报工作 2010年第15期54卷 144-146,134页

作者：郭国庆贵州民族学院贵阳550025

介绍《玉函山房藏书簿录》著录图书总量为:4602部、58622卷,其中有复本236部、983卷。指出马国翰注意收藏清朝诗文别集,藏书"兼收并蓄、细大不捐",不仅易类、礼类、春秋类、地理类、正史类、儒家类等著述居于主要地位,谱录类、小说家类... 详细信息

介绍《玉函山房藏书簿录》著录图书总量为:4602部、58622卷,其中有复本236部、983卷。指出马国翰注意收藏清朝诗文别集,藏书"兼收并蓄、细大不捐",不仅易类、礼类、春秋类、地理类、正史类、儒家类等著述居于主要地位,谱录类、小说家类、杂家类、医方类、阴阳家类的著述也收藏颇丰;且他重视乡邦及宦游之地的文献收藏。类书的收藏在马国翰藏书中占有重要地位。

关键词：马国翰藏书种类数量《玉函山房藏书簿录》