Antibody to collapsin response mediator protein 1 promotes neurite outgrowth from rat hippocampal neurons

作     者：Hongsheng Lin Jing Chen Wenbin Zhang Xiaobing Gong Biao Chen Guoqing Guo 

作者机构：Department of Orthopedics the First Affiliated Hospital of Jinan University Guangzhou 510630 Guangdong Province China Department of Anatomy Medical College of Jinan University Guangzhou 510630 Guangdong Province China 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2011年第6卷第20期

页      面：1537-1542页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071006[理学-神经生物学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：Guangdong Provincial Science and Technology Foundation, No.2010B031600102,2010-170-1 Guangdong Provincial Medical Science Foundation, No. A2008344 Macao Science and Technology Foundation, No.026-2010-A 

主　　题：collapsin response mediator protein 1 neuron microtubule neurite antibody block hippocampus rat neural regeneration 

摘      要：This study examined the role of collapsin response mediator protein 1 （CRMP-1） on neurite outgrowth from rat hippocampal neurons by blocking its function using an antibody. Hippocampal neurons, cultured in vitro, were treated （blocked） using a polyclonal antibody to CRMP-1, and neurite outgrowth and cytoskeletal changes were captured using atomic force microscopy and laser confocal microscopy. Control cells, treated with normal rabbit IgG, established their characteristic morphology and had a large number of processes emerging from the soma, including numerous branches. Microtubules were clearly visible in the soma, formed an elaborate network, and were aligned in parallel arrays to form bundles which projected into neurites. After blocking with CRMP-1 antibody, the number of branches emerging from axons and dendrites significantly increased and were substantially longer, compared with control cells. However, the microtubule network nearly disappeared and only a few remnants were visible. When CRMP-1 antibody-blocked neurons were treated with the Rho inhibitor, Y27632, numerous neurites emerged from the soma, and branches were more abundant than in control neurons. Although the microtubules were not as clearly visible compared with neurons cultured in control medium, the microtubule network recovered in cells treated with Y27632, when compared with cells that were blocked by CRMP-1 antibody （but not treated with Y27632）. These results demonstrate that neurite outgrowth from hippocampal neurons can be promoted by blocking CRMP-1 with a polyclonal antibody.