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World Journal of Gastroenterology 2008年第26期14卷 4204-4208页

作者： Zheng-Hao Tang Xiao-Hua Chen Yong-Sheng Yu guo-qing zang Department of Infectious Diseases Sixth People's Hospital Affiliated to Shanghai Jiaotong University Shanghai200233 China

AIM:To explore the prevalence of SEN virus (SENV) in patients with non A-E hepatitis and volunteer blood donors in Shanghai. METHODS: According to the published gene sequences, primers from the conserved region were designed. Then, the prevalence of SEN virus in 30 samples from healthy voluntary blood donors and 30 samples from patients with non A-E hepatitis were detected by nested-PCR of SENV-D/H. Some PCR products were cloned and sequenced. RESULTS: The specificity of genotype-specific PCR was confirmed by sequencing, the SENV DNA was detected in 53.3% of the patients with non A-E hepatitis and 10% of the blood donors. The prevalence of SENV-D/H viremia was significantly higher in patients with non A-E hepatitis than in blood donors (P = 0.0002). SENV-H subtype and SENV-D subtype were found in 2 and 1 samples, respectively from blood donors. SENV-H subtype, SENV D subtype, mixed SENV-D and SENV-H subtype were found in 8, 6 and 2 samples, respectively, from patients with non A-E hepatitis. CONCLUSION: The gene type of SENV in patients with non A-E hepatitis and blood donors in shanghai is D or H subtype, and transfusion is not the only transmitting form of SENV.

关键词： SEN virus Nested PCR Blood donor NonA-E hepatitis

Expression of ICAM-1, HLA-DR, and CD80 on peripheral circulating CD_1αDCs induced in vivo by IFN-αin patients with chronic hepatitis B

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World Journal of Gastroenterology 2006年第9期12卷 1447-1451页

作者： Yong-Sheng Yu Zheng-Hao Tang Jing-Chao Han Min Xi Jie Feng guo-qing zang Department of Infectious Diseases Sixth People's Hospital of Shanghai Jiaotong University Shanghai 200233 China

AIM: To explore the effects of interferon-α(IFN-α) application on peripheral circulating CD1αdendritic cells (DCs) in patients with chronic hepatitis B, and the expression of HLA-DR, CD80, and ICAM-1 on CD1αDCs in order to explore the mechanism of immune modulation of IFN-α. METHODS: By flow cytometry technique, changes of CD1αDCs were monitored in 22 patients with chronic hepatitis B treated with IFN-αand in 16 such patients not treated with IFN-αwithin three months. Meanwhile, the expression of HLA-DR, CD80, and ICAM-1 on CD1αDCs was detected. RESULTS: In the group of IFN-αtreatment, the percentage of CD1αDCs in peripheral blood mono-nuclear cells was increased after three months of therapy. In patients who became negative for HBV-DNA after IFN-αtreatment, the increase of DCs was more prominent, while in control, these changes were not observed. Increased expression of HLA-DR, CD80, and ICAM-1 on CD1αDCs was also observed. CONCLUSION: CD1αDCs can be induced by IFN-αin vivo, and the immune related molecules such as HLA-DR, CD80, and ICAM-1 are up-regulated to some degree. This might be an important immune related mechanism of IFN-αtreatment for chronic hepatitis B.

关键词： Chronic hepatitis B DC Immune costimulatory molecules Immunotherapy IFN-α

Enhancement of CTLs induced by DCs loaded with ubiquitinated hepatitis B virus core antigen

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World Journal of Gastroenterology 2012年第12期18卷 1319-1327页

作者： Jian-Hua Chen Yong-Sheng Yu Xiao-Hua Chen Hong-Hong Liu guo-qing zang Zheng-Hao Tang Department of Infec-tious Diseases Sixth People's Hospital Affiliated to ShanghaiJiaotong UniversityShanghai 200233China

AIM： To investigate whether hepatitis B virus （HBV） could induce a hepatitis B virus core antigen （HBcAg）specific cytotoxic T lymphocyte （CTL） response in vitro by dendritic cells （DCs） transduced with lentiviral vector-encoding ubiquitinated hepatitis B virus core antigen （LV-Ub-HBcAg）.METHODS： Recombinant LV-Ub-HBcAg were transfected into highly susceptible 293 T cells to obtain high virus titres, Bone marrow-derived DCs isolated from BALB/c mice were cultured with recombinant granulocyte-macrophage colony-stimulating factor and recombinant interleukin （IL）-4. LV-Ub-HBcAg, lentiviral vector-encoding hepatitis B virus core antigen （LV-HBcAg）, lentiviral vector （LV） or lipopolysaccharide were added to induce DC maturation, and the DC phenotypes were analyzed by flow cytometry. The level of IL-12 in the supernatant was detected by enzyme-linked immunosorbent assay （ELISA）. T lymphocytes were proliferated using Cell Counting Kit-8. DCs were cultured and induced to mature using different LVs, and co-cultured with allogeneic T cells to detect the secretion levels of IL-2, IL-4, IL-10and interferon-γ in the supernatants of T cells by ELISA. Intracellular cytokines of proliferative T cells were analyzed by flow cytometry, and specific CTL activity was measured by a lactate dehydrogenase release assay.RESULTS： LV-Ub-HBcAg-induced DCs secreted more IL-12 and upregulated the expression of CD80, CD86 and major histocompatibility class ]I, DCs sensitised by different LVs effectively promoted cytokine secretion; the levels of IL-2 and interferon-y induced by LV-Ub- HBcAg were higher than those induced by LV-HBcAg, Compared with LV-HBcAg-transduced DCs, LV-Ub- HBcAg-transduced DCs more efficiently stimulated the proliferation of T lymphocytes and generated HBcAgspecific cytotoxic T lymphocytes.CONCLUSION： LV-Ub-HBcAg effectively induced DC maturation. The mature DCs efficiently induced T cell polarisation to Thl and generated HBcAg-specific CTLs.

关键词： Ubiquitin Hepatitis B virus core antigen Lentiviruses Dendritic cells Cytotoxic T lymphocytes

Effect of hepatocyte apoptosis induced by TNF-α on acute severe hepatitis in mouse models

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World Journal of Gastroenterology 2000年第5期6卷 688-692页

作者： guo qing zang Xia Qiu Zhou Hong Yu qing Xie guo Ming Zhao Bin Wang qing guo Yue Qin Xiang Dan Liao Department of Infectious Diseases,Rujin Hospital,Shanghai Second Medical University,Shanghai 200025,China 上海第二医科大学上海

AIM To study the effect of hepatocyteapoptosis and necrosis induced by TNF-α on thepathogenesis of acute severe hepatitis(ASH).METHODS The model of ASH was prepared inD-galactosamine(GAIN)sensitized BALB/c miceby injection of either endotoxin(ET)or tumornecrosis factor-α(TNF-α).Morphologicalchanges of apoptotic hepatocytes were studiedby both light and electron microscope and in siteend labeling method(ISEL).Molecular biologicalchanges of DNA ladder were observed byelectrophoresis of extract from liver *** changes were measured by alanineaminotransferase(ALT),asparticaminotransferase(AST)and TNF-α.The relationbetween apoptosis and necrosis was *** The sequence of hepatocyteapoptosis,necrosis,and final death from ASHwas observed both in GAIN/ET and GAIN/*** was prominent at 3.5 h and 5 hafter injection of inducer,while necrosis becamedominant at 9 h after *** appearanceof apoptosis was earlier in GAIN/TNF-α groupthan that in GAIN/ ET *** ofmice with antiTNF IgG1 may completely preventthe liver injury induced by GalN/*** TNF-α can cause liver damageby inducing hepatic apoptosis and necrosis inmice with endotoxemia.

关键词： tumor necrosis factor hepatitis apoptosis alanine transaminase aspartate transaminase endotoxins mice

Research on light dark matter particle from evaporating primordial black hole in CDEX-10 experiment

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Science China(Physics,Mechanics & Astronomy) 2024年第10期67卷 168-169页

作者： qing-guo Huang School of Fundamental Physics and Mathematical Sciences Hangzhou Institute for Advanced StudyUCASHangzhou 310024China CAS Key Laboratory of Theoretical Physics Institute of Theoretical PhysicsChinese Academy of SciencesBeijing 100190China School of Physical Sciences University of Chinese Academy of SciencesBeijing 100049China

Dark matter is an essential component of the universe,accounting for approximately 85%of all ***,what the nature of dark matter actually is remains a huge *** far,all evidence for the existence of dark matter has come from gravitational *** gravitational interactions are universal,it is challenging to reveal the nature of dark matter through gravitational *** unravel the mystery of dark matter,it is essential to directly detect dark matter.

关键词： approximate particle gravitational

Escape probability for isotropic emitters near Kerr black hole with astrometric

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Communications in Theoretical Physics 2024年第5期76卷 128-133页

作者： Yu-Xuan Han qing-Hua Zhu qing-guo Huang CAS Key Laboratory of Theoretical Physics Institute of Theoretical PhysicsChinese Academy of SciencesBeijing 100190China School of Physical Sciences University of Chinese Academy of SciencesNo.19A Yuquan RoadBeijing 100049China Department of Physics Chongqing UniversityChongqing 401331China School of Fundamental Physics and Mathematical Sciences Hangzhou Institute for Advanced Study UCASHangzhou 310024China

The paper investigates the escape probability for isotropic emitters near a Kerr black *** propose a new approach to obtain the escape probability in a general manner,going beyond previous case-by-case *** approach is based on studies of the black hole shadow with astrometric observable and can be applied to emitters with an arbitrary 4-velocities and locations,even to the emitters outside of the equatorial *** also consider representative examples illustrating how escape probabilities vary with distance,velocity,and inclination ***,this new approach provides an effective method for studying escape probabilities near Kerr black holes.

关键词： Kerr black hole photon escape probability isotropic emitters

Spatial light modulation for femtosecond laser manufacturing:Current developments and challenges

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Science China(Technological Sciences) 2024年第1期67卷 60-72页

作者： zang Xue LIU ZiTing XU YiShi WANG Yi WANG qing LI ZhenZe WANG Lei State Key Laboratory of Integrated Optoelectronics College of Electronic Science and EngineeringJilin UniversityChangchun 130012China State Key Laboratory of Precision Measurement and Instruments Department of Precision InstrumentTsinghua UniversityBeijing 100084China

Since the invention of lasers,spatial-light-modulated laser processing has become a powerful tool for various *** enables multidimensional and dynamic modulation of the laser beam,which significantly improves the processing efficiency,accuracy,and flexibility,and presents wider prospects over traditional mechanical technologies for machining three-dimensional,hard,brittle,or transparent *** this review,we introduce:(1)The role of spatial light modulation technology in the development of femtosecond laser manufacturing;(2)the structured light generated by spatial light modulation and its generation methods;and(3)representative applications of spatial-light-modulated femtosecond laser manufacturing,including aberration correction,parallel processing,focal field engineering,and polarization ***,we summarize the present challenges in the field and possible future research.

关键词： femtosecond laser manufacturing spatial light modulation structured light aberration correction parallel processing focal field engineering polarization control

Prescribed Performance Tracking Control of Time-Delay Nonlinear Systems With Output Constraints

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IEEE/CAA Journal of Automatica Sinica 2024年第7期11卷 1557-1565页

作者： Jin-Xi Zhang Kai-Di Xu qing-guo Wang IEEE the State Key Laboratory of Synthetical Automation for Process Industries Northeastern UniversityShenyang 110819China Institute of Artificial Intelligence and Future Networks Beijing Normal UniversityZhuhai 519087 Guangdong Key Laboratory of Artificial Intelligence and Multi-Modal Data Processing Beijing Normal University-Hong Kong Baptist University United International CollegeZhuhai 519087

The problem of prescribed performance tracking control for unknown time-delay nonlinear systems subject to output constraints is dealt with in this paper. In contrast with related works, only the most fundamental requirements, i.e., boundedness and the local Lipschitz condition, are assumed for the allowable time delays. Moreover, we focus on the case where the reference is unknown beforehand, which renders the standard prescribed performance control designs under output constraints infeasible. To conquer these challenges, a novel robust prescribed performance control approach is put forward in this ***, a reverse tuning function is skillfully constructed and automatically generates a performance envelop for the tracking error. In addition, a unified performance analysis framework based on proof by contradiction and the barrier function is established to reveal the inherent robustness of the control system against the time delays. It turns out that the system output tracks the reference with a preassigned settling time and good accuracy,without constraint violations. A comparative simulation on a two-stage chemical reactor is carried out to illustrate the above theoretical findings.

关键词： Nonlinear systems output constraints prescribed performance reference tracking time delays

Exploring a new path of green and efficient utilization of sinter return fine to produce composite pellets

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Journal of Iron and Steel Research International 2024年第7期31卷 1610-1622页

作者： Yan-biao Chen Wen-guo Liu Hao guo Jing-song Wang qing-guo Xue Hai-bin Zuo State Key Laboratory of Advanced Metallurgy University of Science and Technology BeijingBeijing 100083China MCC Capital Engineering and Research Incorporation Limited Beijing 100176China

Efficient utilization of sinter return fine is an important measure to reduce cost,increase efficiency,save energy and reduce emission.A new path of green and efficient utilization of return fine was proposed to produce composite *** metallurgical properties of composite pellets under the condition of hydrogen-rich blast furnace were *** experimental results indicate that the coated concentrate was consolidated for the composite pellets through normal Fe_(2)O_(3) *** the surface of core return fine,the liquid phase formed due to its low-melting point,assimilated the adjacent concentrate,and then consolidated with the temperature *** with regular pellets,the com-pressive strength and reduction swelling index of composite pellets were decreased,but the reducibility index and softening-melting properties were *** addition,the reduction degradation index of composite pellets was sig-nificantly higher than that of ***,adding composite pellets was conducive to indirect reduction in blast furnace,reducing fuel ratio and improving production *** to the effect of the roasting system on the metallurgical properties,the roasting temperature and time were determined as 1250℃and 30 min,*** composite pellets can be produced under the traditional pelletizing process.

关键词： Sinter Return fine Composite pellet Roasting system Metallurgical property Hydrogen-rich blast furnace

Cosmological interpretation for the stochastic signal in pulsar timing arrays

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Science China(Physics,Mechanics & Astronomy) 2024年第4期67卷 43-50页

作者： Yu-Mei Wu Zu-Cheng Chen qing-guo Huang School of Fundamental Physics and Mathematical Sciences Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesHangzhou 310024China School of Physical Sciences University of Chinese Academy of SciencesBeijing 100049China Department of Astronomy Beijing Normal UniversityBeijing 100875China Advanced Institute of Natural Sciences Beijing Normal UniversityZhuhai 519087China Department of Physics and Synergistic Innovation Center for Quantum Effects and Applications Hunan Normal UniversityChangsha 410081China CAS Key Laboratory of Theoretical Physics Institute of Theoretical PhysicsChinese Academy of SciencesBeijing 100190China

The pulsar timing array(PTA)collaborations have recently reported compelling evidence for a stochastic signal consistent with a gravitational-wave *** this paper,we combine the latest data sets from NANOGrav,PPTA,and EPTA to explore cosmological interpretations for the PTA signal from first-order phase transitions,domain walls,and cosmic strings,*** find that the domain wall model is strongly disfavored with the Bayes factors compared with the first-order phase transitions and cosmic strings being 1/90 and 1/189,respectively,breaking the degeneracy among these models in individual data *** also find that:(1)a strong phase transition at temperatures below the electroweak scale is favored,and the bubble collisions make the dominant contribution to the energy density spectrum;(2)a small reconnection probability p<1.55×10^(-1)allowed by strings in(super)string theory is favored at the 95%confidence level,and ground-based detectors can further constrain the parameter space.

关键词： puslar timing arrays gravitational-wave background first-order phase transitions domain walls cosmic strings