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Repairing whole facial nerve defects with xenogeneic acellular nerve grafts in rhesus monkeys

Neural Regeneration Research 2022年第5期17卷 1131-1137页

作者： guo-chen zhu Da-Jiang Xiao Bi-Wen zhu Yan Xiao Department of Otorhinolaryngology-Head and Neck Surgery Affiliated Wuxi No.2 People’s Hospital of Nanjing Medical UniversityWuxiJiangsu ProvinceChina Department of Otorhinolaryngology-Head and Neck Surgery Affiliated Wuxi Clinical College of Nantong UniversityWuxiJiangsu ProvinceChina College of Animal Science&Technology Zhongkai University of Agriculture and EngineeringGuangzhouGuangdong ProvinceChina Department of Pathology Affiliated Wuxi No.2 People’s Hospital of Nanjing Medical UniversityWuxiJiangsu ProvinceChina

Acellular nerve allografts conducted via chemical extraction have achieved satisfactory results in bridging whole facial nerve defects clinically,both in terms of branching a single trunk and in connecting multiple branches of an extratemporal segment.However,in the clinical treatment of facial nerve defects,allogeneic donors are limited.In this experiment,we exposed the left trunk and multiple branches of the extratemporal segment in six rhesus monkeys and dissected a gap of 25 mm to construct a monkey model of a whole left nerve defect.Six monkeys were randomly assigned to an autograft group or a xenogeneic acellular nerve graft group.In the autograft group,the 25-mm whole facial nerve defect was immediately bridged using an autogenous ipsilateral great auricular nerve,and in the xenogeneic acellular nerve graft group,this was done using a xenogeneic acellular nerve graft with trunk-branches.Examinations of facial symmetry,nerve-muscle electrophysiology,retrograde transport of labeled neuronal tracers,and morphology of the regenerated nerve and target muscle at 8 months postoperatively showed that the faces of the monkey appeared to be symmetrical in the static state and slightly asymmetrical during facial movement,and that they could actively close their eyelids completely.The degree of recovery from facial paralysis reached House-Brackmann grade II in both groups.Compound muscle action potentials were recorded and orbicularis oris muscles responded to electro-stimuli on the surgical side in each monkey.Fluoro Gold-labeled neurons could be detected in the facial nuclei on the injured side.Immunohistochemical staining showed abundant neurofilament-200-positive axons and soluble protein-100-positive Schwann cells in the regenerated nerves.A large number of mid-graft myelinated axons were observed via methylene blue staining and a transmission electron microscope.Taken together,our data indicate that xenogeneic acellular nerve grafts from minipigs are safe and effective for repairing whole facial nerve defects in

关键词： acellular nerve compound muscle action potentials facial nerve defect facial symmetry monkey nerve graft nerve regeneration retrograde labeling test synaptophysin xenogeneic

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Impact of Alcian blue and periodic acid Schiff expression on the prognosis of gastric signet ring cell carcinoma

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World Journal of Gastrointestinal Oncology 2024年第3期16卷 687-698页

作者： Juan Lin zhu-Feng chen guo-Dong guo Xin chen Shengli Clinical Medical College Fujian Medical UniversityFuzhou 350001Fujian ProvinceChina Department of Pathology Fujian Provincial HospitalFuzhou 350001Fujian ProvinceChina Department of Internal Medicine Fujian Provincial HospitalFuzhou 350001Fujian ProvinceChina

BACKGROUND The Alcian blue(AB)and periodic acid Schiff(PAS)stains are representative mucus markers in gastric signet ring cell carcinoma(SRCC).They are low-cost special staining methods used to detect acidic mucus and neutral mucus,respectively.However,the clinical importance of the special combined AB and PAS stain is unclear.AIM To investigate AB expression,PAS expression and the AB-to-PAS(A/P)ratio in gastric SRCC patients and to assess patient prognosis.METHODS Paraffin-embedded sections from 83 patients with gastric SRCC were stained with AB and PAS,and signet ring cell positivity was assessed quantitatively.Immuno-histochemical staining for Ki67,protein 53(P53)and human epidermal growth factor receptor 2(HER2)was performed simultaneously.The cancer-specific survival(CSS)rate was estimated via Kaplan-Meier analysis.Cox proportional hazards models were used for univariate and multivariate survival analyses.RESULTS Kaplan-Meier survival analysis revealed that the 3-year CSS rate was significantly greater in the high-PAS-expression subgroup than in the low-PAS-expression subgroup(P0.5 group(P=0.042).Univariate Cox regression analysis revealed that the factors affecting prognosis included tumor diameter,lymph node metastasis,vessel carcinoma embolus,tumor stage,the A/P ratio and the expression of Ki67,P53 and the PAS.Cox multivariate regression analysis confirmed that low PAS expression[hazard ratio(HR)=3.809,95%confidence interval(CI):1.563-9.283,P=0.003]and large tumor diameter(HR=2.761,95%CI:1.086-7.020,P=0.033)were independent risk factors for poor prognosis.CONCLUSION A/P>0.5 is potentially a risk factor for prognosis,and low PAS expression is an independent risk factor in the prognosis of gastric SRCC.PAS expression and the A/P ratio could help in predicting the clinical prognosis of patients with SRCC.

关键词： Alcian blue Periodic acid-Schiff Prognosis Gastric Signet ring cell carcinoma

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The journey towards physiology and pathology: Tracing the path of neuregulin 4

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Genes & Diseases 2024年第2期11卷 687-700页

作者： Min chen Jieying zhu Hongyang Luo Wangjing Mu Liang guo School of Exercise and Health and Shanghai Frontiers Science Research Base of Exercise and Metabolic Health Shanghai University of SportShanghai 200438China

Neuregulin 4 (Nrg4),an epidermal growth factor (EGF) family member,can bind to and activate the ErbB4 receptor tyrosine kinase.Nrg4 has five different isoforms by alternative splicing and performs a wide variety of functions.Nrg4 is involved in a spectrum of physiological processes including neurobiogenesis,lipid metabolism,glucose metabolism,thermogenesis,and angiogenesis.In pathological processes,Nrg4 inhibits inflammatory factor levels and suppresses apoptosis in inflammatory diseases.In addition,Nrg4 could ameliorate obesity,insulin resistance,and cardiovascular diseases.Furthermore,Nrg4 improves non-alcoholic fatty liver disease (NAFLD) by promoting autophagy,improving lipid metabolism,and inhibiting cell death of hepatocytes.Besides,Nrg4 is closely related to the development of cancer,hyperthyroidism,and some other diseases.Therefore,elucidation of the functional role and mechanisms of Nrg4 will provide a clearer view of the therapeutic potential and possible risks of Nrg4.

关键词： Angiogenesis Cardiovascular diseases Inflammation Insulin resistance Lipid and glucose metabolism NAFLD Neurobiogenesis Obesity

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Acupotomy alleviates knee osteoarthritis in rabbit by regulating chondrocyte mitophagy via Pink1-Parkin pathway

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Journal of Traditional Chinese Medicine 2024年第3期44卷 468-477页

作者： zhu Wenting guo Changqing DU Mei MA Yunxuan CUI Yongqi chen Xilin guo Changqing the Third Affiliated Hospital of Beijing University of Chinese Medicine School of Acupuncture-Moxibustion and Tuina Beijing University of Chinese Medicine Department of Medical Technology Shijiazhuang Medical College

OBJECTIVE: To investigate the effect of acupotomy, on mitophagy and the Pink1-Parkin pathway in chondrocytes from rabbits with knee osteoarthritis(KOA).METHODS: A KOA model was established via the modified Videman method. Rabbits were randomly divided into a control group(CON), KOA group and KOA + acupotomy group(Acu). Rabbits in the acupotomy group were subjected to acupotomy for 4 weeks after model establishment. The behavior of the rabbits before and after intervention was recorded. Cartilage degeneration was evaluated by optical microscopy and fluorescence microscopy. The level of mitophagy was evaluated by transmission electron microscopy,immunofluorescence and enzyme-linked immunosorbent assay(ELISA). The expression of phosphatase and tensin homolog(PTEN)-induced kinase 1(Pink1)-Parkin mitophagy pathway components was evaluated by immunofluorescence, Western blotting and real-time polymerase chain reaction.RESULTS: In rabbits with KOA, joint pain, mobility disorders and cartilage degeneration were observed, the Mankin score was increased, collagen type Ⅱ(Col-Ⅱ)expression was significantly decreased, mitophagy was inhibited, mitochondrial function was impaired, and factors associated with the Pink1-Parkin pathway were inhibited. Acupotomy regulated the expression of Pink1-Parkin pathway-related proteins, the mitophagy-related protein microtubule-associated protein-1 light chain-3,the translocase of the outer membrane, and the inner mitochondrial membrane 23; increased the colocalization of mitochondria and autophagosomes; promoted the removal of damaged mitochondria;restored mitochondrial adenosine-triphosphate(ATP) production;and alleviated cartilage degeneration in rabbits with KOA.CONCLUSIONS: Acupotomy played a role in alleviating KOA in rabbits by activating mitophagy in chondrocytes via the regulation of proteins that are related to the Pink1-Parkin pathway.

关键词： PTEN phosphohydrolase acupuncture therapy cartilage mitophagy osteoarthritis, knee

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BET inhibitors enhance the anti-cancer effect of etoposide by suppressing the MRN-ATM axis in the DNA damage response

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Genes & Diseases 2024年第1期11卷 19-22页

作者： Zhenhai Li Wenchao Xu Feng chen Jun Zhang Wei-guo zhu Guangdong Key Laboratory of Genome Instability and Human Disease Prevention Department of Biochemistry and Molecular BiologyShenzhen University School of MedicineShenzhenGuangdong 518055China School of Pharmaceutical Sciences Shenzhen University Health Science CenterShenzhenGuangdong 518055China Base for International Science and Technology Cooperation:Carson Cancer Stem Cell Vaccines R&D Center International Cancer CenterShenzhen University Health Science CenterShenzhenGuangdong 518055China Shenzhen Bay Laboratory Shenzhen University School of MedicineShenzhenGuangdong 518055China Marshall Laboratory of Biomedical Engineering Shenzhen University School of MedicineShenzhenGuangdong 518055China

Etoposide is widely used for cancer chemotherapy in the clinic.However,long-term etoposide treatment can lead to adverse effects or drug resistance.To improve the situation,we evaluated the therapeutic efficiency of etoposide combined with inhibitors of bromodomain and extraterminal(BET)family proteins,which have recently emerged as novel anti-cancer targets due to their critical roles in cancer development.Firstly,we showed BRD4,one of the main targets of BET inhibitors,was involved in DNA damage response(DDR)via the homologous recombination(HR)repair pathway.

关键词： etoposide damage cancer

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Artemisia argyi extract subfraction exerts an antifungal effect against dermatophytes by disrupting mitochondrial morphology and function

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Chinese Journal of Natural Medicines 2024年第1期22卷 47-61页

作者： chen Le zhu Yunyun guo Chaowei guo Yujie ZHAO Lu MIAO Yuhuan DU Hongzhi LIU Dahui College of Pharmacy Hubei University of Chinese MedicineWuhan 430065China National Resource Center for Chinese Materia Medica China Academy of Chinese Medical SciencesBeijing 100700China College of Pharmacy Nanjing University of Chinese MedicineNanjing 210023China

Artemisia argyi(A.argyi),a plant with a longstanding history as a raw material for traditional medicine and functional diets in Asia,has been used traditionally to bathe and soak feet for its disinfectant and itch-relieving properties.Despite its widespread use,scientific evidence validating the antifungal efficacy of A.argyi water extract(AAWE)against dermatophytes,particularly Trichophyton rubrum,Trichophyton mentagrophytes,and Microsporum gypseum,remains limited.This study aimed to substantiate the scientific basis of the folkloric use of A.argyi by evaluating the antifungal effects and the underlying molecular mechanisms of its active subfraction against dermatophytes.The results indicated that AAWE exhibited excellent antifungal effects against the three aforementioned dermatophyte species.The subfraction AAWE6,isolated using D101 macroporous resin,emerged as the most potent subfraction.The minimum inhibitory concentrations(MICs)of AAWE6 against T.rubrum,M.gypseum,and T.mentagrophytes were 312.5,312.5,and 625μg·mL−1,respectively.Transmission electron microscopy(TEM)results and assays of enzymes linked to cell wall integrity and cell membrane function indicated that AAWE6 could penetrate the external protective barrier of T.rubrum,creating breaches(“small holes”),and disrupt the internal mitochondrial structure(“granary”).Furthermore,transcriptome data,quantitative real-time PCR(RT-qPCR),and biochemical assays corroborated the severe disruption of mitochondrial function,evidenced by inhibited tricarboxylic acid(TCA)cycle and energy metabolism.Additionally,chemical characterization and molecular docking analyses identified flavonoids,primarily eupatilin(131.16±4.52 mg·g^(−1))and jaceosidin(4.17±0.18 mg·g^(−1)),as the active components of AAWE6.In conclusion,the subfraction AAWE6 from A.argyi exerts antifungal effects against dermatophytes by disrupting mitochondrial morphology and function.This research validates the traditional use of A.argyi and provides scientific support for its antidermatophytic applicati

关键词： Artemisia argyi Dermatophyte Antifungal activity Mitochondrial structure Mitochondrial function Transcriptome analysis

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Multifunction-oriented high-mobility polymer semiconductors

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National Science Review 2024年第3期11卷 16-19页

作者： Mingliang zhu Yunlong guo Yunqi Liu Beijing National Laboratory for Molecular Sciences Organic Solids Laboratory Institute of Chemistry Chinese Academy of Sciences School of Chemical Sciences University of Chinese Academy of Sciences

The discovery of conductive polyacetylene has initiated interdisciplinary research into condensed matter physics and synthetic chemistry in organic electronics. Unlike their inorganic and small-molecule counterparts, polymer semiconductors possess the inherent advantages of facile solution processing,large-area manufacturing, excellent mechanical strength and intrinsic flexibility.

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一种基于变频调速分时复用的复合式振动台及控制系统

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建筑机械 2024年第1期 96-98页

作者：赵静静周风华陈国柱向伟湖南三一快而居住宅工业有限公司湖南长沙410199

本文介绍了一种基于变频调速分时复用的复合式振动台及控制系统,本设备结合水平方向摇晃振动台和垂直方向高频振动台的优点,能够更高效实现混凝土预制构件的夯实处理。

关键词：水平摇晃振动台垂直高频振动台模台流转变频器分时复用

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Revealing the stability origins of 596 days-humidity-stable semitransparentperovskite solar cells

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Journal of Energy Chemistry 2024年第7期94卷 208-216页

作者： Tian chen Ying Yang Congtan zhu Weihuang Lin Qilin Dai Xueyi guo School of Metallurgy and Environment Central South UniversityChangsha 410083HunanChina Department of Chemistry Physics and Atmospheric SciencesJackson State UniversityJacksonMS 39217USA

Hybrid organic-inorganic perovskites(HOIPs)hold promise in the field of optoelectronics due to their excellent photoelectric conversion efficiency.However,the ion migration and hygroscopicity of these perovskite solar cells need to be addressed.Here,we presented semitransparent perovskite solar cells(ST-PSCs)using hole transport layer(HTL)combined with polyaniline(PANI)to stabilize HTL/perovskite interface,achieving a humidity durability(RH,50%-90%)for 596 days(14304 h)without encapsulation.Moreover,the decrease in hydrolysis products(LiF)showed the interaction between PANI with the addi-tives in HTL dramatically inhibited the water uptake and corrosion on MAPbI_(3),layer.The PANI modified samples had a higher I/Pb ratio and lower trap state density,which indicated the passivation effect of PANI on the uncoordinated Pb^(2+)and iodine vacancies.Subsequently,PANI successfully stabilized the interface and perovskite by inhibiting the formation of Pb^(0) and Au migration as long period storage.This work presented an interfacial design to develop HOiP in air with high humidity stability.

关键词： HTL/perovskite interface Polyaniline-passivation Humidity-degradation mechanism lon migration

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Polyethylene interfacial dielectric layer for organic semiconductor single crystal based field-effect transistors

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Chinese Chemical Letters 2024年第4期35卷 431-435页

作者： Min chen Boyu Peng Xuyun guo Ye zhu Hanying Li Department of Polymer Science and Engineering MOE Key Laboratory of Macromolecular Synthesis and FunctionalizationInternational Research Center for X PolymersShanxi-Zheda Institute of Advanced Materials and Chemical EngineeringZhejiang UniversityHangzhou 310027China Department of Applied Physics Research Institute for Smart EnergyThe Hong Kong Polytechnic UniversityHong Kong 999077China

Organic semiconductor single crystals(OSSCs) have shown their promising potential in high-performance organic field-effect transistors(OFETs). The interfacial dielectric layers are critical in these OFETs as they not only govern the key semiconductor/dielectric interface quality but also determine the growth of OSSCs by their wetting properties. However, reported interfacial dielectric layers either need rigorous preparation processes, rely on certain surface chemistry reactions, or exhibit poor solvent resistance, which limits their applications in low-cost, large-area, monolithic fabrication of OSSC-based OFETs. In this work, polyethylene(PE) thin films and lamellar single crystals are utilized as the interfacial dielectric layers, providing solvent resistive but wettable surfaces that facilitate the crystallization of 6,13-bis(tri-isopropylsilylethynyl)pentacene(TIPS-PEN) and 6,13-bis(triisopropylsilylethynyl)-5,7,12,14-tetraazapentacene(TIPS-TAP). As evidenced by the presence of ambipolar behavior in TIPS-PEN single crystals and the high electron mobility(2.3 ± 0.34 cm^(2)V^(-1)s^(-1)) in TIPS-TAP single crystals, a general improvement on electron transport with PE interfacial dielectric layers is revealed, which likely associates with the chemically inertness of the saturated C-H bonds. With the advantages in both processing and device operation, the PE interfacial dielectric layer potentially offers a monolithic way for the enhancement of electron transport in solution-processed OSSC-based OFETs.

关键词： Polyethylene Interfacial dielectric layer Organic semiconductor Single crystal Organic field-effect transistors

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