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Distribution of the COVID-19 epidemic and correlation with population emigration from Wuhan,china

Chinese Medical Journal 2020年第9期133卷 1044-1050页

作者： Ze-liang Chen Qi Zhang Yi Lu Zhong-Min guo Xi Zhang Wen-Jun Zhang Cheng guo Cong-hui liao Qian-lin li Xiao-hu Han Jia-Hai Lu One Health Center School of Public HealthSun Yat-sen UniversityGuangzhouGuangdong 510080China Key Laboratory of livestock Infectious Diseases in northeast china Ministry of EducationShenyang Agricultural UniversityShenyangLiaoning 110866China college of animal science and Veterinary Medicine Shenyang Agricultural UniversityShenyangLiaoning 110866China Department of Health Law Policy and ManagementSchool of Public HealthBoston UniversityBostonMA 02215USA animal Experiment Center Sun Yat-sen UniversityGuangzhouGuangdong 510080China Department of Biological science and technology School of Life SciencesSun Yat-sen UniversityGuangzhouGuangdong 510080China Center for Infection and Immunity Mailman School of Public HealthColumbia UniversityNew YorkNY 10032USA

Background:The ongoing new coronavirus pneumonia(Corona Virus Disease 2019,COVID-19)outbreak is spreading in china,but it has not yet reached its *** million people emigrated from Wuhan before lockdown,potentially representing a source of virus *** case distribution and its correlation with population emigration from Wuhan in the early stage of the epidemic is of great importance for early warning and for the prevention of future ***:The official case report on the COVID-19 epidemic was collected as of January 30,*** and location information on COVID-19 cases was extracted and analyzed using ArcGIS and WinBUGS *** on population migration from Wuhan city and hubei province were extracted from Baidu Qianxi,and their correlation with the number of cases was ***:The COVID-19 confirmed and death cases in hubei province accounted for 59.91%(5806/9692)and 95.77%(204/213)of the total cases in china,*** spot provinces included Sichuan and Yunnan,which are adjacent to *** time risk of hubei province on the following day was 1.960 times that on the previous *** number of cases in some cities was relatively low,but the time risk appeared to be continuously *** correlation coefficient between the provincial number of cases and emigration from Wuhan was up to *** lockdown of 17 cities in hubei province and the implementation of nationwide control measures efficiently prevented an exponential growth in the number of ***:The population that emigrated from Wuhan was the main infection source in other cities and *** cities with a low number of cases showed a rapid increase in case *** to the upcoming Spring Festival return wave,understanding the risk trends in different regions is crucial to ensure preparedness at both the individual and organization levels and to prevent new outbreaks.

关键词： Corona Virus Disease 2019 Temporal Spatial Distribution Outbreak

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Molecular cloning and Expression Patterns of a Calmodulin Gene MCaM-3 From Mulberry (Morus L.)

Molecular cloning and Expression Patterns of a Calmodulin Ge...

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江苏省遗传学会第八届会员代表大会暨学术研讨会

作者： Fang Rong-Jun~(a,b) hu Dong-Qing~(a,c) Zhao Wei-guo~(a,c*) liu li~a Cheng Jia-ling~a Qi Jin-liang~b Yang Yong-hua~b (a.Sericultural Research Institute,Chinese Academy of agricultural sciences,Zhenjiang Jiangsu 212018,china b School of life sciences,Nanjing university,Nanjing Jiangsu 210093,china c college of biological and Environmental Engineering, Jiangsu university of science and technology,Zhenjiang Jiangsu 212003,china )

Calmodulin(CaM) is a multifunctional Ca-binding protein in eukaryotic cells,it plays important roles in regulating the growth and development,environment adaptation and stress tolerance in plant. A full-length cDNA sequence coding CaM of mulberry tree,which we designated MCaM-3(GeneBank accession No:GQ303247),was cloned based on the ESTs from mulberry *** sequence analysis showed that the MCaM-3 is 951bp in length,it contains a 143bp 5’-UTR(untranslated region) and a 358bp 3’-UTR,an open reading frame(ORF) of 450bp,encoding 149 amino acids with a predicted molecular weight of 16.85kD and an isoelectric point of *** SMART analysis showed that the protein of MCaM-3 has four EFh functional domains,which can bind *** analysis revealed that,CaM genes are highly conservative in mulberry and other species including Arabidopsis,poplar,soybean,wheat,rice and maize. Phylogenetic analysis based on MCaM-3 and CaM genes in other 19 species showed that mulberry tree shares close relationships with castor oil plant,tobacco and so *** expression level of MCaM-3 at different developmental stages of mulberry leaves and flowers and different sites in mulberry were also investigated,the MCaM-3 transcripts can all be detected with the most abundant expression on mature tissues in mulberry,such as mature female flower and climax *** results of semi-quantitative RT-PCR analysis showed that the expression lever of its mRNA could be increased significantly under the conditions of low temperature,drought and salt stress respectively compared to the normal growth *** results validated the proteins encoded by MCaM-3 may be involved in a general response to abiotic stresses in *** research will help to understand the resistance mechanism of functional genes in mulberry in the future.

关键词： Morus L. MCaM-3 gene Molecular cloning EFh Expression

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Characterization of a novel regulatory pathway for mannitol metabolism and its coordination with biofilm formation in Mycobacterium smegmatis

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Journal of Genetics and Genomics 2018年第9期45卷 477-488页

作者： Jialing hu hua Zhang Si Zhou Weihui li Zheng-guo He National Key Laboratory of agricultural Microbiology College of Life Science and TechnologyHuazhong Agricultural University

Biofilm formation has been implicated to be tightly regulated in bacteria. Mycobacterial species possess a unique cell-wall structure; however, the underlying regulation mechanism for their biofilm formation remains largely unclear. In this study, we characterized a hypothetical mannitol metabolism and transportation gene cluster（Ms5571-Ms5576）, designated as mmt operon, whose expression significantly contributes to the biofilm formation in Mycobacterium smegmatis. We showed that in the operon the Ms5575 gene encodes a GntR-like transcriptional repressor and the Ms5576 gene encodes a mannitol2-dehydrogenase which can produce D-mannitol from D-mannose. Strikingly, the D-mannitol molecule can derepress the negative regulation of Ms5575 on the mmt operon to stimulate the operon＇s expression. Consistently, addition of D-mannitol into the medium can obviously induce mycobacterial biofilm formation. Furthermore, we found that Ms0179 positively regulates the mmt operon through its downstream regulator Ms0180. Ms0180 directly binds the mmt operon to positively regulate its expression. Both Ms0179 and Ms0180 significantly affect the mycobacterial biofilm formation. Taken together, we explored a regulatory pathway for the mannitol metabolism and its coordination with the biofilm formation in M. smegmatis. This finding provides novel insights into the unique mechanism of biofilm formation regulation in mycobacteria.

关键词： Transcription regulation Biofilm formation Mannitol Mycobacteria

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α-酮戊二酸对湖羊精子质量与血浆生化指标的影响

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饲料工业 2024年第11期45卷 55-61页

作者：王赫川崔卫国张涵李天峰尹国安郭庆李井春黑龙江八一农垦大学动物科技学院黑龙江大庆163319

试验旨在研究东北地区秋季饲喂α-酮戊二酸对湖羊精子质量与血浆生化指标的影响,选择体况良好且体重相近的湖羊32只,随机平均分为4组,分别按只补饲0、5、10、20 g/dα-酮戊二酸。试验于8—10月在黑龙江伊春市开展,平均气温10.3℃。预试... 详细信息

试验旨在研究东北地区秋季饲喂α-酮戊二酸对湖羊精子质量与血浆生化指标的影响,选择体况良好且体重相近的湖羊32只,随机平均分为4组,分别按只补饲0、5、10、20 g/dα-酮戊二酸。试验于8—10月在黑龙江伊春市开展,平均气温10.3℃。预试期7 d,正试期56 d。分别于试验第28、42、56天采集湖羊颈静脉血,评估血浆睾酮、褪黑素、白细胞介素(2、4、6、10)、促肾上腺皮质激素、肿瘤坏死因子-α、丙二醛水平、总抗氧化能力及抗氧化酶活性。采集湖羊精液检测精子活率、精子质膜完整率及精子畸形率。结果表明:α-酮戊二酸对湖羊精子畸形率、血浆睾酮、白细胞介素(2、6、10)水平无显著影响(P>0.05)。每只湖羊补饲20 g/dα-酮戊二酸显著提高了精子活率、精子质膜完整率、血浆褪黑素水平、总抗氧化能力及超氧化物歧化酶、谷胱甘肽过氧化物酶及过氧化氢酶活性(P<0.05),同时显著降低了血浆丙二醛、促肾上腺皮质激素、白细胞介素-4及肿瘤坏死因子-α水平(P<0.05)。说明东北地区秋季每只湖羊补饲20 g/dα-酮戊二酸能够改善精子质量、血浆生殖激素水平、抗氧化能力与免疫指标水平。

关键词： α-酮戊二酸湖羊精子质量抗氧化免疫指标

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猪链球菌靶向DC重组蛋白DC3pep-SDZ对斑马鱼的免疫保护性分析

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中国兽医杂志 2024年第4期60卷 35-42页

作者：于恩远荣睿璠曾君赵瑞利郭海悦张东超胡烨李颖王心如天津农学院动物科学与动物医学学院天津市农业动物繁育与健康养殖重点实验室天津西青300392 天津市动物疫病预防控制中心天津北辰300402

为了探究本课题组前期构建的由猪链球菌(SS)的分泌型DNA核酸酶A(SsnA)、二氢硫辛酰胺脱氢酶(DLDH)和高亲和力锌摄取系统蛋白(ZnuA)3个毒力因子的优势细胞表位和树突状细胞靶向肽(DC3pep)串联而成的重组蛋白DC3pep-SDZ(SsnA-DLDH-ZnuA)... 详细信息

为了探究本课题组前期构建的由猪链球菌(SS)的分泌型DNA核酸酶A(SsnA)、二氢硫辛酰胺脱氢酶(DLDH)和高亲和力锌摄取系统蛋白(ZnuA)3个毒力因子的优势细胞表位和树突状细胞靶向肽(DC3pep)串联而成的重组蛋白DC3pep-SDZ(SsnA-DLDH-ZnuA)对致病型SS攻击斑马鱼的免疫保护效果,本试验表达和纯化重组蛋白DC3pep-SDZ和SDZ,分别免疫AB系野生型斑马鱼,攻击致病型2、3和9型SS(SS2、SS3和SS9),计算存活率,采用苏木精-伊红(H.E.)染色法观察斑马鱼脑组织病理变化,实时荧光定量PCR(RT-qPCR)分析SS3感染斑马鱼后脑组织炎性细胞因子表达量变化。结果显示,诱导表达的重组蛋白DC3pep-SDZ和SDZ分子质量分别为44和42 kDa,2个蛋白均以可溶的形式表达。SS2、SS3和SS9攻击斑马鱼后,重组蛋白DC3pep-SDZ组和重组蛋白SDZ组斑马鱼存活率分别为50.0%、65.0%和60.0%,45.0%、47.3%和45.0%,两组间差异不显著(P>0.05),但与PBS对照组相比差异均极其显著(P1)。H.E.染色镜检结果显示,重组蛋白DC3pep-SDZ组的斑马鱼被3种分型SS攻击后脑组织均无明显病理变化。RT-qPCR结果显示,重组蛋白DC3pep-SDZ组促炎细胞因子:与PBS对照组相比较IL-6、TNF-α和IFN-γmRNA表达量分别极显著(P1)、极其显著(P1)和极显著(P1)下调,与重组蛋白SDZ组相比较IL-6和TNF-αmRNA表达量分别极显著(P1)和显著下调(P10和TGF-β1 mRNA表达量分别极显著(P1)和极其显著(P1)上调,与重组蛋白SDZ组相比较TGF-β1 mRNA表达量显著上调(P<0.05)。结果表明,重组蛋白DC3pep-SDZ较SDZ具有更好的免疫原性,能产生SS2、SS3和SS9交叉免疫保护作用,同时还能够有效缓解斑马鱼感染SS3后的脑部炎症。

关键词：猪链球菌斑马鱼表位蛋白 DC3pep-SDZ 免疫保护

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三角帆蚌CypA基因的克隆及其在嗜水气单胞菌感染后的表达分析

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水生生物学报 2024年第5期48卷 859-866页

作者：夏虎雷婷杨品红刘良国余嘉张运生邓以国陈福艳陈秀荔贺欢乐湖南文理学院生命与环境科学学院湖南省水生生物资源沅江澧水监测站微生物技术创新团队环洞庭湖水产健康养殖及加工湖南省重点实验室常德415000 农业农村部中国(广西)-东盟水产种质资源综合开发与利用重点实验室(部省共建) 广西壮族自治区水产科学研究院南宁530021 五强溪镇农业综合服务中心怀化418000

为研究亲环蛋白A(Cyclophilin A,CypA)在三角帆蚌(Hyriopsis cumingii)免疫系统中的作用,研究利用PCR与RACE技术克隆获得三角帆蚌CypA基因全长cDNA序列(基因序列登录号:MN121742)。三角帆蚌CypA基因cDNA序列全长1237 bp,其5′非编码区(U... 详细信息

为研究亲环蛋白A(Cyclophilin A,CypA)在三角帆蚌(Hyriopsis cumingii)免疫系统中的作用,研究利用PCR与RACE技术克隆获得三角帆蚌CypA基因全长cDNA序列(基因序列登录号:MN121742)。三角帆蚌CypA基因cDNA序列全长1237 bp,其5′非编码区(UTR)长度为57 bp,3′非编码区长度为688 bp,三角帆蚌CypA基因的开放阅读框(Open Reading Frame,ORF)长度为492 bp,编码164个氨基酸,CypA蛋白质理论等电点为8.9,相对分子量为17.29 kD。在GenBank中进行同源序列检索,三角帆蚌CypA氨基酸序列与其他贝类CypA的相似性依次为池蝶蚌(Hyriopsis schlegelii)(99.39%)、青蛤(Cyclina sinensis)(77.44%)、菲律宾蛤仔(Ruditapes philippinarum)(78.05%)、近江牡蛎(Crassostrea ariakensis)(78.66%)和大竹蛏(Solen grandis)(75.05%)。通过SWISS-MODEL预测三角帆蚌CypA含有8个β折叠和2个α螺旋,可能形成CypA的活性中心区域。利用MEGA 5.1软件将三角帆蚌CypA与其他物种CypA基因进行序列比对并构建系统进化树,分析发现三角帆蚌CypA与池蝶蚌的CypA聚为一支,其与池蝶蚌CypA具有最近的亲源关系。通过qRT-PCR检测CypA基因在三角帆蚌不同组织中表达,发现三角帆蚌性腺中CypA基因的表达量最高,肝脏、肾和鳃中的表达量次之。对三角帆蚌浸泡感染嗜水气单胞菌(Aeromonas hydrophila),CypA基因在肝脏、性腺、肠和鳃中的表达水平在感染72h先升高后降低,其表达量在性腺和鳃中6h最高,肝脏中12h达到峰值,肠中24h达到最高值,表明三角帆蚌感染嗜水气单胞菌后,可显著诱导CypA基因的表达。CypA基因在性腺中的表达量到达峰值的时间要早于肠与肝脏,且到达峰值时的相对表达量(14.91)也显著高于肝脏(9.89)、肠(9.78)和鳃(7.53),推测CypA在三角帆蚌性腺中具有防御细菌感染的重要作用,三角帆蚌性腺可能不仅具有生殖的功能,同时在免疫防御系统中也发挥着重要的作用。

关键词：亲环蛋白A 嗜水气单胞菌免疫应答三角帆蚌

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Dek219 encodes the DICER-liKE1 protein that affects chromatin accessibility and kernel development in maize

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Journal of Integrative Agriculture 2023年第10期22卷 2961-2980页

作者： XIE Si-di tian Ran ZHANG Jun-jie liU Han-mei li Yang-ping hu Yu-feng YU guo-wu huANG Yu-bi liU Ying-hong State Key Laboratory of Crop Gene Exploration and Utilization in Southwest china Sichuan Agricultural UniversityChengdu 611130P.R.China college of life science Sichuan Agricultural UniversityYa’an 625014P.R.China college of Agronomy Sichuan Agricultural UniversityChengdu 611130P.R.China Maize Research Institute Sichuan Agricultural UniversityChengdu 611130P.R.China

Chromatin accessibility plays a vital role in gene transcriptional ***,the regulatory mechanism of chromatin accessibility,as well as its role in regulating crucial gene expression and kernel development in maize(Zea mays)are poorly *** this study,we isolated a maize kernel mutant designated as defective kernel219(dek219),which displays opaque endosperm and embryo ***219 encodes the DICER-liKE1(DCL1)protein,an essential enzyme in mi RNA *** of function of Dek219 results in significant reductions in the expression levels of most mi RNAs and histone *** research showed that the Heat shock transcription factor17(Hsf17)-Zm00001d016571 module may be one of the factors affecting the expression of histone *** results for transposase-accessible chromatin sequencing(ATAC-seq)indicated that the chromatin accessibility of dek219 is altered compared with that of wild type(WT),which may regulate the expression of crucial genes in kernel *** analyzing differentially expressed genes(DEGs)and differentially accessible chromatin regions(ACRs)between WT and dek219,we identified 119 candidate genes that are regulated by chromatin accessibility,including some reported to be crucial genes for kernel *** together,these results suggest that Dek219 affects chromatin accessibility and the expression of crucial genes that are required for maize kernel development.

关键词： maize kernel development chromatin accessibility histone miRNA

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利用X射线CT技术研究根系三维构型的进展

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植物营养与肥料学报 2024年第2期30卷 375-384页

作者：刘玲玲房焕魏晓庆刘玉婷高伟达李保国周虎中国农业大学土地科学与技术学院/农业农村部华北耕地保育重点实验室北京100193 河海大学农业科学与工程学院江苏南京210098

【目的】X射线CT扫描具有非破坏性、高分辨率等特点,近年来被广泛应用于研究植物根系三维构型。本文介绍了X射线CT技术研究植物根系的原理和方法,并重点阐述了CT图像提取和定量根系的方法。【主要进展】X射线CT可以穿透土体进而获取土... 详细信息

【目的】X射线CT扫描具有非破坏性、高分辨率等特点,近年来被广泛应用于研究植物根系三维构型。本文介绍了X射线CT技术研究植物根系的原理和方法,并重点阐述了CT图像提取和定量根系的方法。【主要进展】X射线CT可以穿透土体进而获取土体三维结构,并能通过图像分析准确定量土体内部的细节特征(如根系、孔隙等)。目前根系结构研究中应用的CT主要有医用CT、工业显微CT、同步辐射CT等,分别用于研究不同大小和分辨率要求的样品。应用CT扫描根系样品时,样品的制备和扫描参数的选择非常重要,样品的尺寸、容器的材质、扫描功率和扫描时长等因素都会影响CT图像质量。CT图像中提取根系的方法包括全局阈值法、区域生长法、根系追踪法、深度学习法等,结合图像预处理和后处理能更完整地提取根系。利用ImageJ、Rootrak、RooTh等软件,可以定量根系形态学参数(体积、根长、直径等),还可以分析根系三维分布特征(根系生长角度、根系生长深度等)。【展望】从CT图像中提取根系的算法在实际应用中仍存在很多问题,开发提取根系的先进算法,准确获取根系三维构型并将其定量表征,仍将是CT技术用于根系研究的重要方向。由于CT设备性能的特点,扫描样品尺寸和图像分辨率的矛盾仍然是关键问题,X射线CT硬件设备的创新和发展对其在根系研究中更加广泛和有效的应用具有重要意义。

关键词：根系三维构型 X射线CT 图像分割根系定量化

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Preparation and Identification of Anti-rabies Virus Monoclonal Antibodies

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Virologica Sinica 2012年第3期27卷 172-178页

作者： Wen-juan wang Xiong li li-hu Shan Lei Cao Peng-cheng Yu Qing Tang guo-dong liang college of animal science and Veterinary Medicine Qingdao Agricultural universityQingdao 266109China State Key Laboratory for Infectious Disease Prevention and ControlInstitute forViral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China Xinjiang Center for Disease Control and Prevention Xinjiang 830001China

To provide a foundation for the development of rapid and specific methods for the diagnosis of rabies virus infection, anti-rabies virus monoclonal antibodies were prepared and rabies virus nucleoprotein and human rabies virus vaccine strain (PV strain) were used as immunogens to immunize 6-8 week old female BALB/c mice. Spleen cells and SP2/0 myeloma cells were fused according to conventional methods: the monoclonal cell strains obtained were selected using the indirect immunofluorescence test; this was followed by preparation of monoclonal antibody ascitic fluid; and finally, systematic identification of subclass, specificity and sensitivity was carried out. Two high potency and specific monoclonal antibodies against rabies virus were obtained and named 3B12 and 4A12, with ascitic fluid titers of 1:8000 and 1:10000, respectively. Both belonged to the IgG2a subclass. These strains secrete potent, stable and specific anti-rabies virus monoclonal antibodies, which makes them well suited for the development of rabies diagnosis reagents.

关键词： Rabies virus Monoclonal antibodies Specificity Detection

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Key indicators affecting maize stalk lodging resistance of different growth periods under different sowing dates

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Journal of Integrative Agriculture 2020年第10期19卷 2419-2428页

作者： wang Qun XUE Jun CHEN Jiang-lu FAN Ying-hu ZHANG guo-qiang XIE Rui-zhi MING Bo HOU Peng wang Ke-ru li Shao-kun Key Laboratory of Oasis Eco-Agriculture Xinjiang Production and Construction Corps/agricutura1 CollegeShihezi UniversityShihezi 832000P.R.China Institute of Crop sciences Chinese Academy of Agricultural Sciences/Key Laboratory of Crop Physiology and EcologyMinistry of Agriculture and Rural AffairsBejjing 100081P.R.China Research Institute of agricultural sciences Sixth Division of Xinjiang Production and Construction CorpsWujiaqu 831300P.R.China chuxiong State Research and Extension Institute of agricultural science Chuxiong 675000P.R.China

The accurate evaluation of maize stalk lodging resistance in different growth periods enables timely management of lodging risks and ensures stable and high maize ***,we established five diferent sowing dates to create diferent conditions for maize *** evaluated the effects of the different growth conditions on lodging resistance by determining stalk morphology,moisture content,mechanical strength and dry matter,and the relationship between stalk breaking force and these indicators during the silking stage(R1),milk stage(R3),physiological maturity stage(R6),and 20 days after *** height at R1 positively affected stalk breaking *** R3,the cofficient of ear height and the dry weight per unit length of basal internodes were key indicators of stalk lodging *** R6,the key indicators were the coefficient of the center of gravity height and plant fresh *** R6,the key indicator was the coefficient of the center of gravity *** crushing strength of the fourth internode correlated significanty and positively with the stalk breaking force from R1 to R6,which indicates that crushing strength is a reliable indicator of stalk mechanical *** results suggest that high stalk strength and low ear height beneft lodging resistance prior to *** and after R6,the cofficient of the center of gravity height and the mechanical strength of basal internodes can be used to evaluate plant lodging resistance and the appropriate time for harvesting in fields with a high lodging risk.

关键词： maize lodging resistance stalk strength growth periods breaking force

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