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Variation of microbiological and small molecule metabolite profiles of Nuodeng ham during ripening by high-throughput sequencing and GC-TOF-MS

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Food Science and Human Wellness 2024年第4期13卷 2187-2196页

作者： Cong Li Yingling Zou Guozhou Liao Zijiang Yang Dahai Gu Yuehong Pu Changrong Ge guiying wang Livestock Product Processing and Engineering Technology Research Center of Yunnan Province Yunnan Agricultural UniversityKunming 650201China College of Food Science and Technology Yunnan Agricultural UniversityKunming 650201China

The internal microbial diversity and small molecular metabolites of Nuodeng ham in different processing years(the first,second and third year sample)were analyzed by high-throughput sequencing technology and gas chromatography-time of flight mass spectrography(GC-TOF-MS)to study the effects of microorganisms and small molecular metabolites on the quality of ham in different processing years.The results showed that the dominant bacteria phyla of Nuodeng ham in different processing years were Proteobacteria and Firmicutes,the dominant fungi phyla were Ascomycota and Basidiomycota,while Staphylococcus and Aspergillus were the dominant bacteria and fungi of Nuodeng ham,respectively.Totally,252 kinds of small molecular metabolites were identified from Nuodeng ham in different processing years,and 12 different metabolites were screened through multivariate statistical analysis.Further metabolic pathway analysis showed that 23 metabolic pathways were related to ham fermentation,of which 8 metabolic pathways had significant effects on ham fermentation(Impact>0.01,P<0.05).The content of L-proline,phenyllactic acid,L-lysine,carnosine,taurine,D-proline,betaine and creatine were significantly positively correlated with the relative abundance of Staphylococcus and Serratia,but negatively correlated with the relative abundance of Halomonas,Aspergillus and Yamadazyma.

关键词： Nuodeng ham Microbial diversity Small molecule metabolites High-throughput sequencing Gas chromatography-time of flight mass spectrography

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Improvement in bioconversion efficiency and reduction of ammonia emission by introduction of fruit fermentation broth in a black soldier fly larvae and kitchen waste conversion system

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Insect Science 2023年第4期30卷 975-990页

作者： Lusheng Li Lifei Chen Rongsheng Shang guiying wang Jibin Zhang School of Agricultural Science and Engineering Engineering Research Center of Shandong Province for Black Soldier Fly Breeding and Organic Waste ConversionLiaocheng UniversityLiaochengShandong ProvinceChina State Key Laboratory of Agricultural Microbiology National Engineering Research Centre of Microbial PesticidesCollege of Life Science and TechnologyCollege of Animal Science&TechnologyHuazhong Agricultural UniversityWuhanChina

The black soldier fly(BSF),Hermetia illucens(Diptera:Stratiomyidae),is an insect commonly used for the bioconversion of various organic wastes.Not only can the BSF convert organic waste into macromolecular organic substances,such as insect pro-teins,but it can also lessen the pollution associated with these waste products by reducing ammonia emissions,for example.In this study,we measured the effects of adding fruit fermentation broth(Fer)and commercial lactic acid bacteria fermentation broth(Em)to kitchen waste(KW),as deodorizing auxiliary substances,on the growth performance of black soldier fly larvae(BSFL),the intestinal flora structure of BSFL,the ammonia emis-sion from the KW substrate,and the microbial community structure of the KW substrate.We found that the addition of Fer or Em increased the body weight of BSFL after 6 d of culture,increasing the growth rate by 9.96%and 7.96%,respectively.The addition of Fer not only reduced the pH of the KW substrate but also increased the relative abundance of probiotics,such as Lactobacillus,Lysinibacillus,and Vagococcus,which inhibited the growth of ammonifiers such as Bacillus,Oligella,Paenalcaligenes,Paenibacillus,Pseu-dogracilibacillus,and Pseudomonas,resulting in the reduction of ammonia emission in the KW substrate.Moreover,the addition of Fer or Em significantly increased the rela-tive abundances of Bacteroides,Campylobacter,Dysgonomonas,Enterococcus,and Ig-natzschineria in the gut of BSFL and increased the species diversity and richness in the K W substrate.Our findings provide a novel way to improve the conversion rate of organic waste and reduce the environmental pollution caused by BSF.

关键词： ammonia emissions black soldier fly fruit fermentation broth growth per-formance kitchen waste lactic acid bacteria fermentation broth

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Effect of intra-operative chemotherapy with 5-fluorouracil and leucovorin on the survival of patients with colorectal cancer after radical surgery: a retrospective cohort study

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Chinese Medical Journal 2023年第7期136卷 830-839页

作者： Xuhua Hu Zhaoxu Zheng Jing Han Baokun Li Ganlin Guo Peiyuan Guo Yang Yang Daojuan Li Yiwei Yan Wenbo Niu Chaoxi Zhou Zesong Meng Jun Feng Bin Yu Qian Liu guiying wang The Second Department of General Surgery The Fourth Hospital of Hebei Medical UniversityShijiazhuangHebei 050001China Department of Colorectal Surgery National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021China Department of Medical Oncology The Fourth Hospital of Hebei Medical UniversityShijiazhuangHebei 050001China Department of Cancer Institute The Fourth Hospital of Hebei Medical UniversityShijiazhuangHebei 050001China Department of Pediatrics The Fourth Hospital of Hebei Medical UniversityShijiazhuangHebei 050001China Department of General Surgery The Second Hospital of Hebei Medical UniversityShijiazhuangHebei 050050China

Background:The effect of intra-operative chemotherapy(IOC)on the long-term survival of patients with colorectal cancer(CRC)remains unclear.In this study,we evaluated the independent effect of intra-operative infusion of 5-fluorouracil in combination with calcium folinate on the survival of CRC patients following radical resection.Methods:1820 patients were recruited,and 1263 received IOC and 557 did not.Clinical and demographic data were collected,including overall survival(OS),clinicopathological features,and treatment strategies.Risk factors for IOC-related deaths were identified using multivariate Cox proportional hazards models.A regression model was developed to analyze the independent effects of IOC.Results:Proportional hazard regression analysis showed that IOC(hazard ratio[HR]=0.53,95%confidence intervals[CI][0.43,0.65],P<0.001)was a protective factor for the survival of patients.The mean overall survival time in IOC group was 82.50(95%CI[80.52,84.49])months,and 71.21(95%CI[67.92,74.50])months in non-IOC group.The OS in IOC-treated patients were significantly higher than non-IOC-treated patients(P<0.001,log-rank test).Further analysis revealed that IOC decreased the risk of death in patients with CRC in a non-adjusted model(HR=0.53,95%CI[0.43,0.65],P<0.001),model 2(adjusted for age and gender,HR=0.52,95%CI[0.43,0.64],P<0.001),and model 3(adjusted for all factors,95%CI 0.71[0.55,0.90],P=0.006).The subgroup analysis showed that the HR for the effect of IOC on survival was lower in patients with stage II(HR=0.46,95%CI[0.31,0.67])or III disease(HR=0.59,95%CI[0.45,0.76]),regardless of pre-operative radiotherapy(HR=0.55,95%CI[0.45,0.68])or pre-operative chemotherapy(HR=0.54,95%CI[0.44,0.66]).Conclusions:IOC is an independent factor that influences the survival of CRC patients.It improved the OS of patients with stages II and III CRC after radical surgery.Trial registration:***,ChiCTR 2100043775.

关键词： Colorectal cancer Intra-operative chemotherapy Overall survival Retrospective cohort study Stage

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谈催化氧化脱硫技术在处理乙烯碱渣中的应用

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石油化工安全环保技术 2024年第2期40卷 61-64,I0004页

作者：王桂英中石化股份有限公司天津分公司烯烃部天津300270

乙烯装置在生产过程中产生的废碱液中硫化钠浓度≤1%,送高含盐水装置去除硫化钠后,再进入污水处理场处理合格后外排。2021年由于原料变化,废碱液中硫化物浓度增加到达2%~3%,超过高含盐水装置的处理能力,对高含盐水装置的运行造成不利影... 详细信息

乙烯装置在生产过程中产生的废碱液中硫化钠浓度≤1%,送高含盐水装置去除硫化钠后,再进入污水处理场处理合格后外排。2021年由于原料变化,废碱液中硫化物浓度增加到达2%~3%,超过高含盐水装置的处理能力,对高含盐水装置的运行造成不利影响。为解决乙烯废碱液中硫化物浓度高的问题,2022年利用武汉俊恒科技有限公司的低温低压催化氧化脱硫技术对乙烯废碱液中硫化物进行了预处理,硫化物浓度由10000~30000mg/L降低至200mg/L以下,确保了高含盐水装置的正常运行。

关键词：乙烯废碱液低温低压催化脱硫

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Variation of microbiological and small molecule metabolite profiles of Nuodeng ham during ripening by high-throughput sequencing and GC-TOF-MS

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Food Science and Human Wellness 2023年

作者： Cong Li Yingling Zou Guozhou Liao Zijiang Yang Dahai Gu Yuehong Pu Changrong Ge guiying wang Livestock Product Processing and Engineering Technology Research Center of Yunnan Province， Yunnan Agricultural University College of Food Science and Technology， Yunnan Agricultural University

The internal microbial diversity and small molecular metabolites of Nuodeng ham in different processing years (the first， second and third year sample) were analyzed by high-throughput sequencing technology and gas chromatography-time of flight mass spectrography (GC-TOF-MS) to study the effects of microorganisms and small molecular metabolites on the quality of ham in different processing years. The results showed that the dominant bacteria phyla of Nuodeng ham in different processing years were Proteobacteria and Firmicutes， the dominant fungi phyla were Ascomycota and Basidiomycota， while Staphylococcus and Aspergillus were the dominant bacteria and fungi of Nuodeng ham， respectively. 252 kinds of small molecular metabolites were identified from Nuodeng ham in different processing years， and 12 different metabolites were screened through multivariate statistical analysis. Further metabolic pathway analysis showed that 23 metabolic pathways were related to ham fermentation， of which 8 metabolic pathways had significant effects on ham fermentation (Impact > 0.01， P < 0.05). The content of L-proline， phenyllactic acid， L-lysine， carnosine， taurine， D-proline， betaine and creatine were significantly positively correlated with the relative abundance of Staphylococcus and Serratia， but negatively correlated with the relative abundance of Halomonas， Aspergillus and Yamadazyma.

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LincRNA-1614 coordinates Sox2/PRC2-mediated repression of developmental genes in pluripotency maintenance

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Journal of Molecular Cell Biology 2018年第2期10卷 118-129页

作者： Xudong Guo Zikang wang Chenqi Lu Wujun Hong guiying wang Yanxin Xu Zhongmin Liu Jiuhong Kang Clinical and Translational Research Center of Shanghai First Maternity & Infant Health Hospital Shanghai Key Laboratory of Signaling and Disease Research Collaborative Innovation Center for Brain Science School of Life Science and Technology Tongji University Shanghai 200092 China Institute of Regenerative Medicine East Hospital Tongji University School of Medicine Shanghai 200120 China Laboratory of Population and Quantitative Genetics institute of Biostatistics School of Life Sciences Fudan University Shanghai 200433 China

Large-intergenic noncoding RNAs (lincRNAs) cooperate with core transcription factors to coordinate the pluripotency network of embryonic stem cells. The mechanisms by which lincRNAs affect chromatin structure and gene transcription remain mostly unknown. Here, we identified that a lincRNA (linc1614), occupied by pluripotency factors at its promoter, was indispensable for both maintenance and acquisition of pluripotency. Linc1614 served as a specific partner of core factor Sox2 in maintaining pluripotency, primarily by mediating the function of Sox2 in the repression of developmental genes. Moreover, Ezh2, an essential subunit of polycomb repressive complex 2 (PRC2), physically interacted with linc1614 and contributed to lincRNA-mediated transcriptional silencing. Thus, we propose that the interplay of linc1614 with Sox2 implicates this lincRNA as a recruitment platform that mediates transcriptional silencing by guiding the PRC2 complex to the loci of developmental genes.

关键词：基因协调发展维护染色质结构建筑群 RNA 干细胞子单元

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Construction of the first high-density genetic map for growth related QTL analysis in Ancherythroculter nigrocauda

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Journal of Oceanology and Limnology 2021年第3期39卷 1118-1130页

作者： Yanhong SUN Pei LI guiying wang Renli SUN Jian CHEN Qi ZHOU Jingou TONG Qing LI Fisheries Research Institute Wuhan Academy of Agricultural SciencesWuhan 430207China Wuhan Xianfeng Aquaculture Technology Co.Ltd. Wuhan 430207China Wuhan Academy of Agricultural Sciences Wuhan 430207China State Key Laboratory of Freshwater Ecology and Biotechnology Institute of HydrobiologyChinese Academy of SciencesWuhan 430072China

Ancherythroculter nigrocauda is a fish endemic to the upper areas of the Changjiang(Yangtze)River in China.Quantitative trait locus(QTL)mapping is a powerful tool to identify potential genes affecting traits of economic importance in domestic animals.In this study,a high-density genetic map was constructed with 5901 single nucleotide polymorphism(SNP)makers by sequencing 92 individual fish from a F1 family using the specific-locus amplified fragment sequencing approach.Initially,48 QTLs for total length,body length,body height,and body weight were identified according to the high density of the genetic map with 24 LGs,a total length of 3839.4 cM,and marker spacing of about 0.82 cM.These QTLs explained 27.1%-49.9%of phenotypic variance.The results of this study suggest that major QTLs are responsible for the growth of A.nigrocauda,and these are potentially useful in comparative genomics research,genome assembly,and marker-assisted breeding programs for this species.

关键词： Ancherythroculter nigrocauda specific-locus amplified fragment high-density genetic map quantitative trait locus

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盐津乌骨鸡与瓢鸡屠宰性能与肉品质的比较

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食品工业科技 2024年第12期45卷 241-247页

作者：杨源荀文廖国周杨春芳吴红艳葛长荣王桂瑛云南农业大学食品科学技术学院云南昆明650201 云南农业大学云南省畜产品加工工程技术研究中心云南昆明650201

为探讨盐津乌骨鸡与瓢鸡屠宰性能及肉质理化特性差异,以300日龄盐津乌骨鸡与瓢鸡为对象,对两个品种间的屠宰性能、感官与常规理化指标进行测定与比较分析。结果表明:盐津乌骨鸡与瓢鸡均具有良好肉用性能,雌性盐津乌骨鸡的胸肌率显著高... 详细信息

为探讨盐津乌骨鸡与瓢鸡屠宰性能及肉质理化特性差异,以300日龄盐津乌骨鸡与瓢鸡为对象,对两个品种间的屠宰性能、感官与常规理化指标进行测定与比较分析。结果表明:盐津乌骨鸡与瓢鸡均具有良好肉用性能,雌性盐津乌骨鸡的胸肌率显著高于雄性盐津乌骨鸡和瓢鸡(P<0.05);同一品种雄鸡的宰前体重、屠体重、全净膛重、胸肌重、腿肌重和腿肌率高于雌鸡(P<0.05),屠宰性能更好。两个品种鸡肉的外观评分均较高(6.30~7.30分),腿肉较胸肉有更高的香味、质地、滋味和总体接受性评分。两个品种鸡宰后pH均在优质鸡范围内,瓢鸡腿肉的蒸煮损失率、水分含量、粗灰分、粗脂肪均显著高于盐津乌骨鸡(P<0.05)。同一品种不同部位组间,腿肉的蒸煮损失(P<0.05)和粗蛋白含量(P<0.05)低于胸肉;而水分(P<0.05)和粗脂肪(P<0.05)含量高于胸肉。本研究为两种优良地方鸡种的深度开发利用提供科学理论依据,也为云南优质地方鸡品质评价标准的建立奠定基础。

关键词：盐津乌骨鸡瓢鸡屠宰性能肉品质

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基于环境DNA评价鸭绿江口底栖生态质量状况

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海洋学报 2024年第3期46卷 75-88页

作者：王桂营李宏俊邵魁双刘滨玮楚奇叶金清生态环境部国家海洋环境监测中心辽宁大连116023

为研究环境DNA鉴定底栖生物评价生态质量状况的应用潜力,本研究采集17份鸭绿江口底栖生物样品,分别利用环境DNA与形态学进行鉴定,并对所得生态质量评价指数(AMBI、BENTIX、香浓-维纳H'、M-AMBI)进行比较分析。结果显示:环境DNA鉴定生物... 详细信息

为研究环境DNA鉴定底栖生物评价生态质量状况的应用潜力,本研究采集17份鸭绿江口底栖生物样品,分别利用环境DNA与形态学进行鉴定,并对所得生态质量评价指数(AMBI、BENTIX、香浓-维纳H'、M-AMBI)进行比较分析。结果显示:环境DNA鉴定生物隶属于10纲16目19科20属22种,形态学鉴定生物隶属于9纲27目43科55属57种,共有生物10种;两种鉴定方法得出的AMBI指数间(R=0.428,p=0.043,y=0.32x+1.08)、BENTIX指数间(R=0.430,p=0.043,y=0.28x+3.59)存在显著一致性,而香浓-维纳H'指数间存在显著差异性;两种鉴定方法得出的AMBI等级间、M-AMBI等级间相似性较高,分别为51.02%、44.90%;两种鉴定方法得出的AMBI与M-AMBI等级更符合实际情况,且评价鸭绿江口整体生态质量状况为良。本研究表明,基于环境DNA鉴定底栖生物评价生态质量状况,在海洋环境监测调查中具有较高的应用潜力。

关键词：环境DNA 底栖生物生态质量

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A Visual pH Indicator through Purple Cabbage Dye for Freshness Test of Venison

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Journal of New Media 2021年第3期3卷 109-118页

作者： Kaixin Qi guiying wang Yinggang Huang Pengfei Fang College of Engineering&Technology Northeast Forestry UniversityHarbin150040China

A visual pH indicator through purple cabbage dye is selected to test the freshness of venison.Chitosan and cassava starch of an equal weight were used to prepare the film-forming matrix of the indicator.The crystallization of natural purple cabbage dyes with a weight ratio of 5%,10%,20%and 40%were added to the matrix,respectively.The pH color test showed that the natural purple cabbage lyophilized powder with a weight ratio of 40%was the best for the pH indicator,which was used to test the freshness of venison stored at 4℃.The total bacterial count,volatile basic nitrogen(TVB-N)and thiobarbituric acid(TBA)of venison were tested through an experiment.When stored at under 4℃,a significant color change of the indicator from pink to blue-green was shown when the total amount of volatile basic nitrogen(TVB-N)(22.78 mg/100 g)exceeded the critical value(20 mg/100 g).The test result showed that the indicator was very sensitive to pH value and it would help customers identify the freshness of venison.

关键词： Visual pH indicator purple cabbage dye TVB-N TBA venison

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