LincRNA-1614 coordinates Sox2/PRC2-mediated repression of developmental genes in pluripotency maintenance
LincRNA-1614 coordinates Sox2/PRC2-mediated repression of developmental genes in pluripotency maintenance作者机构：Clinical and Translational Research Center of Shanghai First Maternity & Infant Health Hospital Shanghai Key Laboratory of Signaling and Disease Research Collaborative Innovation Center for Brain Science School of Life Science and Technology Tongji University Shanghai 200092 China Institute of Regenerative Medicine East Hospital Tongji University School of Medicine Shanghai 200120 China Laboratory of Population and Quantitative Genetics institute of Biostatistics School of Life Sciences Fudan University Shanghai 200433 China
出 版 物：《Journal of Molecular Cell Biology》 (分子细胞生物学报（英文版）)
年 卷 期：2018年第10卷第2期
页 面：118-129页
核心收录：
学科分类：0710[理学-生物学] 083001[工学-环境科学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1002[医学-临床医学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种]
基 金：This work was supported by grants from the Ministry of Science and Technology (2016YFA0101300) the National Natural Science Foundation of China (81530042 31210103905 31371510 31571529 31571519 31471250 and 31571390) the Science and Technology Commission of Shanghai Municipality (15JC1403201) and the Fundamental Research Funds for the Central Universities (2000219136 and 1500219106)
主 题：lincRNA Sox2 pluripotency maintenance PRC2 reprogramming
摘 要：Large-intergenic noncoding RNAs （lincRNAs） cooperate with core transcription factors to coordinate the pluripotency network of embryonic stem cells. The mechanisms by which lincRNAs affect chromatin structure and gene transcription remain mostly unknown. Here, we identified that a UncRNA （linc1614）, occupied by pluripotency factors at its promoter, was indispensable for both maintenance and acquisition of pluripotency. Linc1614 sewed as a specific partner of core factor Sox2 in maintaining pluripotency, primarily by mediating the function of Sox2 in the repression of developmental genes. Moreover, Ezh2, an essential subunit of polycomb repressive complex 2 （PRC2）, physically interacted with linc1614 and contributed to lincRNA-mediated transcriptional silencing. Thus, we propose that the interplay of linc1614 with Sox2 implicates this lincRNA as a recruitment platform that mediates transcriptional silencing by guiding the PRC2 complex to the loci of developmental genes.
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