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The role of environmental temperature on movement patterns of giant anteaters

Integrative Zoology 2022年第2期17卷 285-296页

作者： Aline GIROUX Zaida ORTEGA Alessandra BERTASSONI Arnaud Léonard Jean deSBIEZ Danilo KLUYBER Gabriel Favero MASSOCATO guilherme de miranda guilherme MOURÃO Luciana SURITA Nina ATTIAS Rita de Cassia BIANCHI Vinícius Peron de Oliveira GASPAROTTO Luiz Gustavo Rodrigues OLIVEIRA-SANTOS Ecology department Federal University of Mato Grosso do Sul(UFMS)Campo GrandeMato Grosso do SulBrazil Post Graduate Program of Ecology and Evolution Federal University of Goiás(UFG)GoiâniaGoiásBrazil Institute of Research and Conservation of Anteaters in Brazil(PROJETO TAMANDUÁ) ParnaíbaPiauíBrazil Institute for the Conservation of Wild Animals(ICAS) Campo GrandeMato Grosso do SulBrazil Royal Zoological Society of Scotland(RZSS) MurrayfieldEdinburghUK Institute for Ecological Research(IPÊ) NazaréPaulistaSão PauloBrazil Naples Zoo at Caribbean Gardens NaplesFloridaUSA The Houston Zoo HoustonTexasUSA National Institute of Criminalistics-Federal Police BrasíliaBrazil Embrapa Pantanal CorumbáMato Grosso do SulBrazil Natural Resources Graduate Program(PRONAT)-Federal University of Roraima(UFRR) Boa VistaRoraimaBrazil Animal Biology Graduate Program Federal University of Mato Grosso do Sul(UFMS)Campo GrandeMato Grosso do SulBrazil Animal Biology department São Paulo State University(UNESP)São Josédo Rio PretoSão PauloBrazil department of Preventive Veterinary Medicine and Animal Health School of Veterinary MedicineUniversity of São Paulo(USP)São PauloSPBrazil Brazilian Institute for Conservation Medicine(TRÍAde) RecifePernambucoBrazil

Mammals can show conspicuous behavioral responses to thermal variation,including changes in movement *** used an integrative approach to understand how environmental temperature can drive the movement behavior of a mammal with low capacity for physiological thermoregulation,the giant anteater(Myrmecophaga tridactyla).We tracked 52 giant anteaters in 7 areas throughout the Brazilian *** estimated the distance moved,area used,use of forest areas,and mean environmental temperature for each monitoring day of each *** modeled these data with Mixed Structural Equations—considering the possible interactions between our variables and controlling for sex and body *** anteaters reduced displacement and increased forest use with decreasing environmental temperature,probably because of their low body heat *** is possible that they reduce distance moved and area used by reducing the duration of *** decreasing temperature,forest habitats become warmer than open ones,besides buffer rain and chilly *** displacement and using forests are important strategies to reduce body heat loss and the energetic costs of ***,decreasing movement can limit food access and,consequently,***,we highlight the importance of forests as thermal *** increasing frequency and intensity of extreme weather events,we showed the need to preserve forest patches to offer suitable conditions for tropical mammals’behavioral *** this context,policies favoring deforestation on Brazilian territory are especially ***,we emphasize the need of integrative approaches to understand the complex interactions between organisms and the environment.

关键词： behavioral thermoregulation biologging habitat use mixed structural equations movement ecology

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Cartilage and bone tissue engineering using adipose stromal/stem cells spheroids as building blocks

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World Journal of Stem Cells 2020年第2期12卷 110-122页

作者： Gabriela S Kronemberger Renata Akemi Morais Matsui guilherme de Almeida Santos de Castro e miranda JoséMauro Granjeiro Leandra Santos Baptista Bioengineering Directory of Metrology Applied to Life SciencesNational Institute of MetrologyQuality and Technology(INMETRO)Duque de CaxiasRJ 25250-020Brazil Post-graduate Program in Translational Biomedicine(Biotrans) UnigranrioCampus IDuque de CaxiasRJ 25250-020Brazil Post-graduate Program in Biotechnology National Institute of MetrologyQuality and Technology(INMETRO)Duque de CaxiasRJ 25250-020Brazil Federal University of Rio de Janeiro(UFRJ) Campus Duque de CaxiasDuque de CaxiasRJ 25250-020Brazil Laboratory of Clinical Research in Odontology Fluminense Federal University(UFF)Niterói 25255-030 Brazil Multidisciplinary Center for Biological Research(Numpex-Bio) Federal University of Rio de Janeiro(UFRJ)Campus Duque de CaxiasDuque de CaxiasRJ 25245-390Brazil

Scaffold-free techniques in the developmental tissue engineering area are designed to mimic in vivo embryonic processes with the aim of biofabricating,in vitro,tissues with more authentic *** clusters called spheroids are the basis for scaffold-free tissue *** this review,we explore the use of spheroids from adult mesenchymal stem/stromal cells as a model in the developmental engineering area in order to mimic the developmental stages of cartilage and bone *** from adult mesenchymal stromal/stem cells lineages recapitulate crucial events in bone and cartilage formation during embryogenesis,and are capable of spontaneously fusing to other spheroids,making them ideal building blocks for bone and cartilage tissue ***,we discuss data from ours and other labs on the use of adipose stromal/stem cell spheroids in chondrogenesis and osteogenesis in ***,recent studies support the notion that spheroids are ideal"building blocks"for tissue engineering by“bottom-up”approaches,which are based on tissue assembly by advanced techniques such as three-dimensional *** studies on the cellular and molecular mechanisms that orchestrate spheroid fusion are now crucial to support continued development of bottom-up tissue engineering approaches such as three-dimensional bioprinting.

关键词： Adipose stromal/stem cells Spheroids Building-blocks Bottom-up developmental tissue engineering Cartilage and bone

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Are models of volumetric production at the diametric distribution and total stand level mutually compatible?

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Journal of Forestry Research 2020年第5期31卷 1691-1698页

作者： Marcos Felipe de Oliveira Valeriano Eder Pereira Miguel Pedro guilherme de Andrade Vasconcelos Mauro Eloi Nappo Humberto Angelo Alba Valeria Rezende Renan Augusto miranda Matias Leonardo Job Biali Ilvan Medeiros Lustosa Junior department of Forestry Engineering University of Brasilia(UnB)Brası´liaBrazil Federal Institute of Brasılia(IFB) BrasıliaBrazil

The objective of this work was to compare estimates generated by a diametric distribution model and a total stand model against the pre-cut *** model efficiency was also *** were evaluated from 30 permanent sample plots in a Eucalyptus urophylla stand,comprising 24 sample plots used for model fitting,and six sample plots for *** volume of wood per hectare was estimated for different productive units(sites),using 7 years as the reference *** model adjustment quality was verified by adjustment and precision statistics:the correlation between observed and predicted variables,root mean square error percentage,graphical analysis of residual distribution,and a frequency histogram for classes of relative errors and *** the two-parameter Weibull probability density function adhered to the data for tree evolution in diameter classes for the reference age(7 years)in the different productivity classes,it generated imprecise estimates of the number of ***,it produced inaccurate volumetric production *** total stand model provided reliable projections of production volumes in different productivity classes for both adjustment types,showing compatibility with the pre-cut inventory according to a Tukey *** summary,the total stand model generated estimates that were compatible with the pre-cut inventory while the diametric distribution model did not.

关键词： Clutter model Diameter class Eucalyptus Prognosis

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Minimally invasive tenodesis for peroneus longus tendon rupture: A case report and review of literature

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World Journal of Orthopedics 2020年第2期11卷 137-144页

作者： Danilo Ryuko Cândido Nishikawa Fernando Aires Duarte guilherme Honda Saito Cesar de Cesar Netto Fábio Correia Paiva Fonseca Bruno Rodrigues de miranda Augusto César Monteiro Marcelo Pires Prado department of Orthopedics Foot and Ankle SurgeryHospital of the Municipal Public Servant of São Paulo(HSPM)Aclimação 01532-000São PauloBrazil Ortocity Orthopaedic Clinic Lapa 05078-000São PauloBrazil department of Orthopaedics Foot and Ankle SurgeryHospital Israelita Albert EinsteinJardim Leonor 05652-900São PauloBrazil department of Orthopedics Foot and Ankle SurgeryUniversity of IowaIowa CityIA 52242United States

BACKGROUND Peroneal tendon disorders are common causes of lateral hindfoot ***,total rupture of the peroneal longus tendon is *** treatment for this condition is usually a side-to-side tenodesis of the peroneal longus tendon to the peroneal brevis *** the traditional procedure involves a long lateral curved incision,this approach is associated with damage to the lateral soft tissues(up to 24%incidence).CASE SUMMARY A 50-year-old female had developed pain at the lateral aspect of the hindfoot 1 mo after an ankle sprain while walking in the *** treatments were anti-inflammatory drugs,ice,rest and Cam-walker *** physical exam,there was pain and swelling over the course of the peroneal *** instability and cavovarus foot deformity were ruled *** strength was weak(4/5).Imaging showed complete rupture of the peroneal longus tendon associated with a sharp hypertrophic peroneal *** repair was indicated after failure of conservative treatment(physiotherapy,rest,analgesics,and ankle stabilizer).A less invasive approach was performed for peroneal longus tendon debridement and side-to-side tenodesis to the adjacent peroneal brevis tendon,with successful clinical and functional *** Peroneus longus tendon tenodesis can be performed through a less invasive approach with preservation of the lateral soft tissue integrity.

关键词： Peroneus longus tendon Complete rupture Tenodesis Minimally invasive surgery Wound breakdown Case report

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Epidemiological and clinical characteristics of COVID-19 in a Brazilian public hospital

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World Journal of Clinical Cases 2023年第8期11卷 1761-1770页

作者： Fillipe Dantas Pinheiro Luana Weber Lopes Rafael Santos Dantas miranda Dórea Glauber Rocha Lima Araújo Filipe Antônio França da Silva Breno Bittencourt de Brito Maria Luísa Cordeiro Santos Geovani Moreno Santos Júnior Maria Teresa Araújo de Lorenzo Barcia Renata de Amorim Marques AndréBezerra Botelho Anna Carolina Saúde Dantas Davi Tanajura Costa Adriano Fernandes Teixeira Cláudio Lima Souza Lucas miranda Marques guilherme Barretos Campos Márcio Vasconcelos Oliveira Dulciene Maria de Magalhães Queiroz Fabrício Freire de Melo Instituto Multidisciplinar em Saúde Universidade Federal da BahiaVitória da Conquista 45029-094BahiaBrazil Hospital Geral de Vitória da Conquista Vitória da Conquista 45030-160BahiaBrazil departamento de Ciências da Saúde Universidade Estadual do Sudoeste da Bahia(UESB)Vitória da Conquista 45083-900BahiaBrazil Faculdade de Medicina Universidade Federal de Minas GeraisBelo Horizonte 31270-901Minas GeraisBrazil

BACKGROUND The coronavirus disease 2019(COVID-19)pandemic has become a major health concern *** that context,the understanding of epidemiological and clinical features associated with the disease and its severity is crucial for the establishment of strategies aimed at disease control and *** To describe epidemiological features,signs,symptoms,and laboratory findings among severely ill COVID-19 patients from an inten-sive care unit in northeastern Brazil as well as to evaluate predictor factors for disease *** This is a prospective single-center study that evaluated 115 patients admitted to the intensive care unit in a northeastern Brazilian *** The patients had a median age of 65.60±15.78 *** was the most frequent symptom,affecting 73.9%of the patients,followed by cough(54.7%).Fever was reported in approximately one-third of patients and myalgia in 20.8%of the *** least two comorbidities were found in 41.7%of the patients,and hypertension was the most prevalent(57.3%).In addition,having two or more comorbidities was a predictor of mortality,and lower platelet count was positively associated with *** and vomiting were two symptoms that were predictors of death,and the presence of a cough was a protective *** This is the first report of a negative correlation between cough and death in severely ill severe acute respiratory syndrome coronavirus 2-infected *** associations between comorbidities,advanced age,and low platelet count and the outcomes of the infection were similar to the results of previous studies,highlighting the relevance of these features.

关键词： COVID-19 Epidemiology Symptoms Comorbidities Laboratory parameters

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Estimation of Genetic Parameters of Turiacu Pineapple Clones and Genetic Correlation between Traits

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Agricultural Sciences 2017年第11期8卷 1253-1262页

作者： guilherme Barbosa Abreu Marcos miranda Toledo Marcelo Mattos Cavallari Jose Ribamar Gusmao Araujo Antonia Alice Costa Rodrigues Luiz Gustavo de Lima Melo Augusto Cesar Vieira Neves Junior Embrapa Cocais(CPACP) Sao LuisBrazil Embrapa Pecuária Sudeste(CPPSE) Sao CarlosBrazil Universidade Estadual do Maranhao(UEMA) Sao LuisBrazil Universidade Federal Rural de Pernambuco(UFRPE) RecifeBrazil

This study aimed to estimate genetic parameters of Turiaçu pineapple clones using mixed models;and estimate genetic correlation coefficient between traits, to provide important information to breeding programs. For this, 19 pineapple plants were visually selected and its slips were collected and planted in a single environment and nine traits were evaluated. The genetic parameters of most evaluated traits presented significant differences between the 19 clones. Some traits, such as Fusarium wilt resistance, showed high magnitudes of heritability. Clones did not differ in relation to total titratable acidity (TTA) and total soluble solids (TSS). However, these traits, which have implications to fruit quality, showed to be negatively correlated to fruit mass (-0.46 and -0.67). The results indicate that the population of Turiaçu pineapple is suitable for selection, especially for Fusarium wilt resistance. Also, Turiaçu clones can be used in breeding programs of pineapple.

关键词： Quantitative Genetics Plant Breeding Ananas comosus

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Schistosoma mansoni Infection in Holochilus sciureus Shows Sex-Related Differences in Parasitological Patterns

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Open Journal of Animal Sciences 2019年第2期9卷 173-182页

作者： guilherme Silva miranda Joao Gustavo Mendes Rodrigues Maria Gabriela Sampaio Lira Ranielly Araujo Nogueira Gleycka Cristine Carvalho Gomes Neuton Silva-Souza department of Parasitology Institute of Biological SciencesFederal University of Minas GeraisBelo HorizonteBrazil Teaching department Federal Institute of MaranhaoSao Raimundo das MangabeirasBrazil department of Pathology Centre for Biological and Health SciencesFederal University of MaranhaoSao LuisBrazil department of Chemistry and Biology Laboratory of Human ParasitologyState University of MaranhaoSao LuisBrazil

Due to the different parasitological patterns found between sexes in human populations and in different biological models during Schistosoma mansoni infection, we proposed to investigate such differences using Holochilus sciureus rodent, besides confirming whether this rodent is suitable for experimental infections. In this sense, males and females of H. sciureus were infected with 200 cercariae from a human strain of S. mansoni. The number of eggs per gram of feces (epg) and the worms were quantified, besides histopathological analysis. Thus, it was shown that females have fewer epg, as well as a longer prepatent period. On the other hand, males had a lower recovery rate of adult worms. The histopathological analysis did not show differences between the sexes. Thus, we suggest that infection in H. sciureus females provides a favorable environment for the development of adult worms, despite impairing the parasite fecundity. In addition, H. sciureus may be an excellent biological model for S. mansoni experimental infections.

关键词： Biological Model Females Males Schistosomiasis Wild Reservoir

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Effect of Growth Regulators in Production and Rooting of Coffea arabica ***

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American Journal of Plant Sciences 2018年第4期9卷 628-636页

作者： Paulo Fernando Marques Cavalcanti Filho Diego Corona Baitelle Sílvio de Jesus Freitas Waldinei Souza da Silva Paulo Cesar dos Santos Weverton Pereira Rodrigues Silverio de Paiva Freitas Ismael Lourenco de Jesus Freitas Tamara Locatelli Abraao Carlos Verdin Filho guilherme Bessa miranda department of Plant Science State University of Northern Rio de JaneiroCampos dos GoitacazesRio de Janeiro StateBrazil

The plants of C. arabica do not naturally produce a large number of orthotropic sprouts, hindering clonal multiplication by cutting, which would be the simplest technique with a lower cost of production compared to other methods of vegetative propagation. An alternative form, used successfully in the propagation of eucalyptus, is propagation by minicutting, which consists of pruning the shoot apex of the plant, forming the ministumps, which in variable time interval emits the shoots that will be used to make minicutting. In C. arabica plants the number of shoots can be increased with the application of growth regulators and fertilizers. So, the purpose of this work was to verify the effect of growth regulators and foliar fertilizers on the emission and development of orthotropic shoots of C. arabica. The experiment was installed in a randomized complete block design, with ten treatments composed by growth regulators and fertilizers: T1 (Control);T2 (Stimulate + Sturdy + Enervig);T3 (Stimulate + Vitakelp + Biozyme);T4 (Tiba + Sturdy + Enervig);T5 (Tiba + Vitakelp + Biozyme);T6 (Brs + Sturdy + Enervig) T7 (Brs + Vitakelp + Biozyme);T8 (Stimulate);T9 (TIBA);T10 (Brs-Brassinoesteroid), each plot consisting of two plants. Biometric and physiological parameters were evaluated for the ministumps, as well as the biometric parameters of the shoots and plant survival. The results showed that there was no influence of the treatments on the biometric parameters of the ministumps. A greater number of orthotopic sprouts were obtained in treatments with the TIBA growth regulator, as well as a greater plant survival of the minicuttings, after 25 days of transplanting in a greenhouse. It was concluded that TIBA treatments influenced the production and quality of clonal minicuttings of C. arabica.

关键词： Arabica Coffee Ministumps Clonal Propagation

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COVID-19: Feelings Experienced by Nurses, Physiotherapists, and Medical Doctors in a Brazilian Field Hospital

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Open Journal of Nursing 2021年第5期11卷 390-406页

作者： Joselito Adriano da Silva Giulia Catissi Leticia Bernardes de Oliveira Daniela Reis Dal Fabbro Michele Jaures Elisiane Plack Gladys Cristina Borges Fábio Racy Andreia Ferreira Nunes Rose Mara miranda denise Tiemi Noguchi Maki guilherme de Paula Pinto Schettino Claudia Regina Laselva Eliseth Ribeiro Leão Hospital Israelita Albert Einstein S&#227 o Paulo Brazil

Facing critical situations such as the ones triggered by COVID-19 may take healthcare professionals to confront their psychological resources, which may induce a higher stress level. We aimed to understand the feelings experienced by nurses, physiotherapists, and medical doctors in a field hospital dedicated to patients with COVID-19 in the biggest city of Brazil. Methods: We performed a qualitative study in a state field hospital in S?o Paulo city, Brazil, in a sample of 25 volunteer healthcare professionals. Participants answered to a sociodemographic electronic survey and were subjected to an individual semi-structured on-site interview. Results: Participants were mostly female (68%), comprised eight nurses, eight physiotherapists, and nine medical doctors, had an average age of 36.8 years, and were professionally experienced. Speech analysis revealed two thematic categories (related to work and assistance in the pandemic) and 11 subcategories: adaptation to the scenario, stressing experience, psychological support, difficulties with workwear, safety with workwear, non-verbal communication, empathy, patient comfort, outstanding experiences, major challenges, and teamwork. Conclusion: Nurses, physiotherapists, and medical doctors’ experiences while working in the field hospital were permeated by both positive and negative feelings. Those feelings originated directly from the fear of the unknown when dealing with a new disease without well-established scientific evidence, the use of medical workwear for long periods, and communication and environmental difficulties. In contrast, positive feelings, related to teamwork and empathy, arose and overlapped with the difficulties.

关键词： Covid-19 Pandemic Healthcare Professionals Field Hospital

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Surgical treatment of cementoblastoma associated with apicoectomy and endodontic therapy:Case report

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World Journal of Clinical Cases 2016年第9期4卷 290-295页

作者： Bernardo Cesar Costa guilherme José Pimentel Lopes de Oliveira Maria das Gra?as Afonso miranda Chaves Renan Roberto da Costa Mário Francisco Real Gabrielli Juliane Maria Guerreiro-Tanomaru Mário Tanomaru-Filho Foar-UNESP Faculdade de Odontologia de AraraquaraUniversidade Estadual Paulista

This case report describes the surgical removal of cementoblastoma associated with apicoectomy and endodontic therapy. The patient, an 18-year-old man, presented pain in the region of the mandibular body on the right side. On clinical exam, bone expansion was observed in the region at the bottom of the vestibular sulcus, pain on palpation, slight extrusion of tooth 46 with presence of pulp vitality. Radiographic exams demonstrated the presence of a radiopaque area and discrete radiolucent halo associated with the root of tooth 46, suggesting the diagnosis of cementoblastoma. Endodontic treatment of tooth 46 was performed and exeresis of the lesion by apicoectomy. Twelve months after the first surgery, recurrence of the lesion was observed, and a new apicoectomy was necessary, this time up to the middle third of the root. Clinical radiographic control 12 mo after the second surgical intervention demonstrated absence of signs and symptoms, radiographic repair, with tooth 46 shown to be fully functional.

关键词： Endodontic treatment Cementoblastoma Apicoectomy

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