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Microhabitat heterogeneity associated with Vanilla *** its influences on the microbial community of leaf litter and soil

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Soil Ecology Letters 2020年第3期2卷 195-208页

作者： Gabriela Montes de Oca-Vasquez Frank Solano-campos Bernal Azofeifa-Bolanos Amelia Paniagua-Vasquez Jose Vega-Baudrit Antonio Ruiz-Navarro Ruben Lopez-Mondejar Felipe Bastida National Nanotechnology Laboratory National Center for High Technology10109 PavasSan JoseCosta Rica School of Biological Sciences Universidad NacionalCampus Omar Dengo86-3000 HerediaCosta Rica Asociacion Nacional de Vainilleros Unidos San JoseCosta Rica Instituto de Investigacion y Servicios Forestales Universidad Nacional86-3000 HerediaCosta Rica Laboratory of Polymer Science and Technology School of ChemistryUniversidad NacionalCampus Omar Dengo86-3000 HerediaCosta Rica CEBAS-CSIC Department of Soil and Water ConservationCampus Universitario de Espinardo30100MurciaSpain Laboratory of Environmental Microbiology Institute of Microbiology of the CASVidenska 1083Praha 414220Czech Republic

The impact of forest microhabitats on physiochemical properties of the soil and that of microbial communities on tropical soils remain poorly *** elucidate the effect of tropical forest stand on leaf litter and soil microbial communities,we studied enzyme activities,microbial biomass,and diversity in three distinct microhabitats in terms of plant richness,diameter at breast height(DBH),and physiochemical properties of soil and litter,each associated with a different Vanilla *** the soil,positive correlations were found between electrical conductivity(EC)and total organic carbon(TOC)with phosphatase activity,and between nitrogen(N)and water-soluble carbon(WSC)content with urease activity(UA).In the litter,the water content was positively correlated with bacterial and fungal biomass,and N and WSC contents were positively correlated with fungal *** correlations were found between plant richness and UA in the soil,plant richness and fungal biomass in the soil and litter,and DBH and fungal biomass in the *** sequencing revealed differences between microhabitats in the relative abundance of some fungal and bacterial taxa and in the bacterial community composition of both litter and *** richness and diversity were different between microhabitats,and,in litter samples,they were negatively correlated with DBH and plant richness,*** contrast,none of the soil and litter physiochemical properties were significantly correlated with microbial *** results show that significant shifts in enzyme activity,microbial biomass,and diversity in the microhabitats were driven by key abiotic and biotic factors depending on the soil or litter sample type.

关键词： Tropical rainforest Enzyme activity Microbial community composition Microbial biomass Plant richness

Salt/Sodium Intake Estimation in Children and Adolescents of Costa Rica

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Food and Nutrition Sciences 2020年第10期11卷 919-941页

作者： Hilda Núñez-Rivas Ileana Holst-Schumacher Adriana Blanco-metzler María de los Angeles Montero-campos Natalia campos-Saborío Karla Benavides-Aguilar Unit of Health and Nutrition of the Costa Rican Institute of Research and Education on Nutrition and Health (INCIENSA) Tres Ríos Costa Rica Faculty of Microbiology University of Costa Rica (UCR) and Research Center for Hematology and Related Disorders (CIHATA) San José Costa Rica Distance State University (UNED) of Costa Rica San José Costa Rica

The objectives of this study were to estimate sodium intake and to identify the main dietary sources of this nutrient in young people. Cross-sectional study performed in 2685 students from 64 schools and high-schools of Costa Rica. A food frequency survey was applied to study the food and beverage intake habits of the participants. The average daily sodium intake for children and adolescents 7 to 18 years of age was 3214 mg. Around 97% of the students had sodium intakes > 2300 mg per day with significant differences by age subgroup, sex and nutritional status due to excess body weight (p meaning for children, adolescents and families, in such a way that these groups of the population become motivated to modify gradually their eating habits, such as reducing the use of sauces, seasonings and salt in food preparation and at the table. If the habit of adding salt to the served food is gradually reduced or avoided, the children and adolescents in Costa Rica could decrease the intake of salt per day from 1 to 2 grams. This decrease could be even more effective if youngsters additionally would remove the intake of sauces, contributing in this way to achieve the maximum salt intake recommendation (5 g per day).

关键词： Salt Sodium Intake Children Adolescents Costa Rica

Violent and Risk-Taking Behaviors Associated with Intake of Energy Drinks Mixed with Alcohol and Perceptions of This Consumption among Children and Adolescents in Costa Rica

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Food and Nutrition Sciences 2020年第8期11卷 836-853页

作者： Núñez-Rivas Hilda Holst-Schumacher Ileana campos-Saborío Natalia Costa Rican Institute of Research and Education in Health and Nutrition (INCIENSA) Tres Ríos Costa Rica University of Costa Rica (UCR) San Pedro Montes de Oca Costa Rica State University at Distance (UNED) Sabanilla Costa Rica

Introduction: The consumption of energy drinks and its mix with alcohol has been associated with negative and violent behaviors in youngsters. Objective: To determine the risk-taking behaviors associated with the consumption of energy drinks mixed with alcohol in Costa Rican adolescents. Methods: A mixed cross-sectional study was performed with a population of 2667 students of 64 educative centers in 2016. Descriptive statistics were performed with the program SPSS 20.0 and a value of p 0.05 was considered significant. Results: The third part of the study population is usually a consumer of energy drinks (30%) and 3% make use of drinks mixed with alcohol. The children and adolescents, who consume energy-alcohol mixed drinks, are more likely to present risk-taking behaviors than those who do not consume these mixed beverages: combined use of medicines to get high;cocaine consumption, consumption of cement/glue, consumption of marijuana, physical violence (fighting), use of weapons, suicidal ideation, deliberate self-harm, low educational expectation, sexual intercourses, cyberbullying and school apathy. The students perceived the intake of energy beverages as of low risk for their health. They also agr

关键词： Energy Drinks Energy-Alcohol Mix Drinks Children Adolescents Costa Rica

Analysis of PTEN methylation Pattern by Pyrosequencing in Renal Cell Carcinoma

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Journal of Cancer Therapy 2020年第12期11卷 793-802页

作者： Eurico Cleto Ribeiro de campos Marcos Gonçalves Adriano Júnior Marcus Rivabem Winheski Renan Martins Ademar Lopes Department of Oncology Surgery of Prevent Senior Sao Paulo Group Sao Paulo Brasil Oncology Surgical Department of Evangélico Mackenzie University Hospital Curitiba Paraná Ponta Grossa State University Paraná Brasil Hospital A. C. Camargo Sao Paulo Brazil

Objectives: We aimed to determine the frequency of PTEN (phosphatase with tensin homology deleted in chromosome 10) hypermethylation in renal cell carcinoma (RCC) and its impact on overall (OS) and disease-free survival (DFS). The association between PTEN hypermethylation and clinic and pathologic factors was also analyzed. Materials and Methods: The authors analyzed 137 patients who had undergone a radical or partial nephrectomy between 1997 and 2011. The methylation pattern was quantified individually at multiple CpG islands that are located on the exon 1 of PTEN gene by pyrosequencing. Results: Mean follow-up was 32.3 months. PTEN hypermethylation was detected with a low frequency (3.6%). There were no deaths or disease recurrence among patients with PTEN hypermethylation. Determining the association between PTEN hypermethylation and clinical and pathological factors was not possible due to the low frequency observed. Conclusion: The results show that the methylation of promoter CpG sites in PTEN is relatively infrequent in renal carcinogenesis and has no prognostic impact on survival.

关键词： methylation PTEN Pyrosequencing Renal Cell Carcinoma

Characterization of goat whey proteins and their bioactivity and toxicity assay

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Food Bioscience 2022年第2期46卷 833-843页

作者： Maria Isabel Ferreira campos Paula Perazzo de Souza Barbosa Laura Junqueira Camargo Luciano Da Silva Pinto Bianca Mataribu Catarina Serr˜ao Luis Fernando Marques-Santos Jos´e Hon´orio Lopes Julia Mariano Caju de Oliveira Carlos Alberto de Almeida Gadelha Tatiane Santi-Gadelha Programa de P´os-Graduaç˜ao em Ciˆencia e Tecnologia de Alimentos Universidade Federal da ParaíbaJo˜ao PessoaParaíbaBrazil Departamento de Biologia Molecular Universidade Federal da ParaíbaJo˜ao PessoaParaíbaBrazil Centro de Desenvolvimento Tecnol´ogico Universidade Federal de PelotasRio Grande do SulBrazil Programa de P´os-Graduaç˜ao em Ciˆencias da Nutriç˜ao Universidade Federal da ParaíbaJo˜ao PessoaParaíbaBrazil

Goat whey is an industry-discarded dairy by-product that has interesting nutritional value and a nutrient composition with important functional potential,where proteins have a high biological value and play an important role in the organism’s ***,the aim of this study was to identify,quantify,concentrate and investigate the capacity of goat whey proteins to perform different biological activities and to evaluate their acute *** proteins were quantified and identified by Bradford,Kjehdal and SDS-PAGE *** activity was performed against Listeria monocytogenes,Salmonella spp.,Staphylococcus aureus,Pseudomonas aeruginosa and Escherichia coli microorganisms and showed MIC ranging from 15 to 120μg mL-1,with the highest inhibition for *** *** activity was performed against human malignant melanoma cells and their proliferation inhibition was up to 66.8%within 72 h of *** highest antioxidant capacity was obtained at concentration 2 mg that showed 29.69%of inhibition of DPPH radical *** acute toxicity test was performed using Artemia franciscana larvae in which whey proteins were able to preserve larval survival throughout ***,we can conclude that goat’s whey has proteins that can play a positive effect on health,with antimicrobial activity and without *** present study highlights the great potential of goat’s whey to be developed as a safe biopreservative or as a functional ingredient in the food industry.

关键词： Goat whey proteins Malignant cells Antimicrobial Antioxidant capacity Whey protein functionality

High frequency of CD34+CD38-/low immature leukemia cells is correlated with unfavorable prognosis in acute myeloid leukemia

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World Journal of Stem Cells 2017年第12期9卷 227-234页

作者： Adriana Plesa Charles Dumontet Eve Mattei Ines Tagoug Sandrine Hayette Pierre Sujobert Isabelle Tigaud Marie Pierre Pages Youcef Chelghoum Fiorenza Baracco Helene Labussierre Sophie Ducastelle Etienne Paubelle Franck Emmanuel Nicolini Mohamed Elhamri Lydia campos Claudiu Plesa Stéphane Morisset Gilles Salles Yves Bertrand Mauricette Michallet Xavier Thomas Laboratory of Hematology Lyon-Sud HospitalHospices Civils de Lyon CRCL INSERM 1052/CNRS 5286 Department of Hematology Hospices Civils de Lyon Laboratory of Hematology Nord Hospital Statistical and Clinical Research Lyon-Sud HospitalHospices Civils de Lyon Department of Pediatric Hematology and BMT IHOP Lyon

AIM To evaluate the importance of the CD34+CD38-cell population when compared to the CD34+CD38+/low and CD34+CD38+/high leukemic cell sub-populations and to determine its correlations with leukemia characteristics and known prognostic factors, as well as with response to therapy and *** Two hundred bone marrow samples were obtained at diagnosis from 200 consecutive patients with newly diagnosed acute myeloid leukemia(AML) were studied between September 2008 and December 2010 at our Institution(Hematology Department, Lyon, France). The CD34/CD38 cell profile was analyzed by multiparameter flowcytometry approach using 8 C panels and FACS CANTO and Diva software(BD Bioscience).RESULTS We analyzed CD34 and CD38 expression in bone marrow samples of 200 AML patients at diagnosis, and investigated the prognostic value of the most immature CD34+CD38-population. Using a cut-off value of 1% of CD34+CD38-from total "bulk leukemic cells" we found that a high(> 1%) level of CD34+CD38-blasts at diagnosis was correlated with advanced age, adverse cytogenetics as well as with a lower rate of complete response after induction and shorter disease-free survival. In a multivariate analysis considering age, leukocytosis, the % of CD34+ blasts cells and the standardized cytogenetic and molecular risk subgroups, a percentage of CD34+CD38-leukemic cells > 1% was an independent predictor of DFS [HR = 2.8(1.02-7.73), P = 0.04] and os [HR = 2.65(1.09-6.43), P = 0.03].CONCLUSION Taken together, these results show that a CD34/CD38 "backbone" for leukemic cell analysis by multicolour flowcytometry at diagnosis provides useful prognostic information.

关键词： CD34+CD38-/low Immunophenotyping Leukemic stem cells Acute myeloid leukemia Prognosis

Conception of a Web Operation System for Processing Petroleum Related Drilling Data: A Focus on Pre-Salt Real-Time Automation and Optimization

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Journal of Software Engineering and Applications 2019年第4期12卷 61-71页

作者： Yuri Soares Pinheiro Lucas campos Vieira Andreas Nascimento Francisco de Assis Souza dos Santos Mauro Hugo Mathias Gerhard Thonhauser Asad Elmgerbi Julian Hunt Department of Engineering and Technology Petroleum Engineering Programs Universidade Federal do Espirito Santo (UFES/DETEC) Sao Mateus Brazil Department of Computational and Electronics Universidade Federal do Espirito Santo (UFES/DCEL) Sao Mateus Brazil Department of Petroleum Engineering Chair of Drilling and Completion Engineering Montanuniversit&#228 t Leoben (MUL/DPE/CDC) Leoben Austria International Institute of Applied System Analysis (IIASA/ASA-ENE) Laxenburg Austria mechanic Department Faculty of Engineering Universidade Estadual Paulista (UNESP/FEG-DME/PRH48-ANP) Guaratingueta Brazil

Petroleum and Natural Gas still represent a considerable share in terms of energy consumption in the current global matrix, so that its exploration/exploitation is present in the market and driving activities in locations of specific complexities, as the ones along unconventional hydrocarbon resources from the Brazilian pre-salt. The daily cost of well drilling under harsh conditions can exceed US $1 million a day, turning any type of downtime or necessary maintenance during the activities to be very costly, moment in which processes optimization starts to be a key factor in costs reduction. Thus, new technologies and methods in terms of automating and optimizing the processes may be of great advantages, having its impact in total related project costs. In this context, the goal of this research is to allow a computation tool supporting achieving a more efficient drilling process, by means of drilling mechanics parameters choosiness aiming rate of penetration (ROP) maximization and mechanic specific energy (MSE) minimization. Conceptually, driven by the pre-operational drilling test curve trends, the proposed system allows it to be performed with less human influences and being updateable automatically, allowing more precision and time reduction by selecting optimum parameters. A Web Operating System (Web os) was designed and implemented, running in online servers, granting accessibility to it with any device that has a browser and internet connection. It allows processing the drilling parameters supplied and feed into it, issuing outcomes with optimum values in a faster and precise way, allowing reducing operating time.

关键词： Trend Curves Web os Optimization Pre-Salt Petroleum

Assessing radiation dose for postoperative radiotherapy in prostate cancer: Real world data

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World Journal of Clinical Oncology 2022年第7期13卷 652-662页

作者： Asunción Hervás-Morón Jose Domínguez-Rullán Victor Duque Santana Mireia Valero Carmen Vallejo Sonsoles Sancho Juan David García Fuentes Miguel Cámara Gallego Fernando López-campos Department of Radiation Oncology Hospital Universitario Ramón Y CajalMadrid 28034Spain Department of medical Physics Hospital Universitario Ramón Y CajalMadrid 28034Spain

BACKGROUND Approximately 30%of patients with localized prostate cancer(PCa)who undergo radical prostatectomy will develop biochemical *** these patients,the only potentially curative treatment is postoperative radiotherapy(PORT)with or without hormone ***,the optimal radiotherapy dose is unknown due to the limited data *** To determine whether the postoperative radiotherapy dose influences biochemical failure-free survival(BFFS)in patients with *** Retrospective analysis of patients who underwent radical prostatectomy for PCa followed by PORT-either adjuvant radiotherapy(ART)or salvage radiotherapy(SRT)-between April 2002 and July *** 2002 to 2010,the prescribed radiation dose to the surgical bed was 66-70 Gy in fractions of 2 Gy;from 2010 until July 2015,the prescribed dose was 70-72 *** were grouped into three categories according to the total dose administered:66-68 Gy,70 Gy,and 72 *** primary endpoint was BFFS,defined as the post-radiotherapy prostatespecific antigen(PSA)nadir+0.2 ng/*** endpoints were overall survival(os),cancer-specific survival(CSS),and metastasis-free survival(MFS;based on conventional imaging tests).Treatment-related genitourinary(GU)and gastrointestinal(GI)toxicity was evaluated according to Radiation Therapy Oncology Group/European Organization for Research and Treatment of Cancer ***,we aimed to identify potential prognostic ***,os,CSS,and MFS were calculated with the Kaplan-meier method and the log-rank *** and multivariate Cox regression models were performed to explore between-group differences in survival outcome *** A total of 301 consecutive patients were *** these,93(33.6%)received ART and 186(66.4%)SRT;22 patients were excluded due to residual macroscopic disease or local recurrence in the surgical *** this subgroup(n=93),43 patients(46.2%)were Gleason score(GS)≤6,44(47.3%)GS 7,and 6(6.5%)GS≥8;clinical stage

关键词： Prostate cancer Postoperative radiotherapy Dose intensified Radiation dose Biochemical relapse free survival

Turbo-taxonomy to assemble a megadiverse lichen genus:seventy new species of Cora(Basidiomycota:Agaricales:Hygrophoraceae),honouring David Leslie Hawksworth’s seventieth birthday

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Fungal Diversity 2017年第3期84卷 139-207页

作者： Robert Lücking Manuela Dal Forno Bibiana Moncada Luis Fernando Coca Leidy Yasmín Vargas-mendoza André Aptroot Laura Juliana Arias Betty Besal Frank Bungartz Diego Mauricio Cabrera-Amaya Marcela E.S.Cáceres José Luis Chaves Sionara Eliasaro Martha Cecilia Gutiérrez Jesús E.Hernández Marin María de los Ángeles Herrera-campos María E.Holgado-Rojas Harald Jonitz Martin Kukwa Fabiane Lucheta Santiago Madriñán Marcelo Pinto Marcelli Suzana Maria de Azevedo Martins Joel A.mercado-Díaz Jorge Alberto Molina Eduardo A.Morales Peter R.Nelson Freddy Nugra Francisco Ortega Telma Paredes Ayda Lucía Patiño Rouchi Nadine Peláez-Pulido Rosa Emilia Pérez Pérez Gary B.Perlmutter Eimy Rivas-Plata Javier Robayo Camilo Rodríguez Diego Fernando Simijaca Edier Soto-medina Adriano Afonso Spielmann Alejandra Suárez-Corredor Jean-Marc Torres Carlos Alberto Vargas Alba Yánez-Ayabaca Gothamie Weerakoon Karina Wilk Marcela Celis Pacheco Mauricio Diazgranados Grischa Brokamp Thomas Borsch Patrick M.Gillevet Masoumeh Sikaroodi James D.Lawrey Botanical Garden and Botanical Museum Berlin KöniginLuise-Straße 6-814195 BerlinGermany Science Education The Field Museum1400 South Lake ShoreChicagoIL 60605USA Department of Botany Smithsonian InstitutionNational Museum of Natural History10th St Constitution Ave NWWashingtonDC 20560USA Department of Environmental Science and Policy George Mason UniversityFairfaxVA 22030-4444USA Licenciatura en Biologı´a Universidad Distrital Francisco Jose´de CaldasCra.4 No.26D-54Torre de LaboratoriosHerbarioBogota´D.C.Colombia Herbario Universidad de Caldas(FAUC) Edificio BicentenarioCalle 65No.26-10ManizalesA.A.275CaldasColombia ABL Herbarium G.v.d.Veenstraat 1073762 XK SoestThe Netherlands Biologı´a Universidad del Quindı´oCarrera 15 Calle 12 NorteArmeniaQuindı´oColombia 120 Chavis Avenue LexingtonVA 24450USA Charles Darwin Foundation Puerto AyoraSanta Cruz IslandGala´pagosEcuador Schedestraße 8 53113 BonnGermany Jardı´n Bota´nico de Bogota´Jose´Celestino Mutis Avenida Calle 63 No.68-95Bogota´D.C.Colombia Departamento de Biocieˆncias Universidade Federal de SergipeAvenida Vereador Olı´mpio Grandes/n.ItabaianaCEP:49.500-000 SergipeBrazil Laboratorio de Hongos Instituto Nacional de Biodiversidad(INBio)Apdo.22-3100Santo Domingo de HerediaCosta Rica Departamento de Botaˆnica Universidade Federal do Parana´Campus Polite´cnicoSetor de Cieˆncias Biolo´gicasAv.Cel.Francisco H.dos Santoss/nJardim das Ame´ricas81531-980 CuritibaPRBrazil Ingenierı´a Ambiental Universidad Distrital Francisco Jose´de CaldasCarrera 5 Este No.15-82Av.CircumvalarBogota´D.C.Colombia Instituto Experimental Jardı´n Bota´nico Dr.Tobı´as Lasser Centro de Investigacio´n y DesarrolloUniversidad Central de VenezuelaAve.Salvador AllendeJardı´n Bota´nico de CaracasApartado#2156Caracas 1010-AVenezuela Departamento de Bota´nica Instituto de Biologı´aUniversidad Nacional Auto´noma de Me´xicoApartado Postal 70-36704510 Ciudad De Me´xicoMexico Facultad de Ciencias Escuela Profesional de Biologı´aUniv

Following a large-scale phylogenetic study of the lichenized genus Cora(Basidiomycota:Agaricales:Hygrophoraceae),we formally describe 70 new species,honouring the seventieth birthday of David Leslie Hawksworth,one of the preeminent figures in mycology and lichenology in the past 50 *** on an updated phylogeny using the ITS fungal barcoding locus,we now recognize 189 taxa in a genus that until recently was considered to represent a single species;including this contribution,92 of these are formally recognized,including five taxa based on historical names or collections that have not been *** of Cora can be recognized by a combination of morphological(size,colour,lobe configuration,surface hairs,hymenophore size and shape),anatomical(thallus thickness,cortex structure,photobiont type,hyphal papillae),and ecogeographical features(substrate,habitat,distribution),and a keytable allowing the identification of all accepted taxa is *** new species are:Cora accipiter Moncada,Madrin˜a´n&Lücking ***.,*** Moncada,Soto-medina&Lücking ***.,*** Moncada,Dal Forno&Lücking ***.,*** Dal Forno,Chaves&Lücking ***.,*** Moncada,***ı´guez&Lücking ***.,*** Lücking,Moncada&*** ***.,*** Moncada,Ya´nez-Ayabaca&Lücking ***.,*** Moncada,Patin˜o&Lücking ***.,*** Lücking,***&Dal Forno ***.,*** Holgado,Rivas Plata&Perlmutter ***.,*** Dal Forno,Eliasaro&Spielmann ***.,*** Nugra,Dal Forno&Lücking ***.,*** Lücking,Martins&Lucheta ***.,*** Moncada,R.-***´rez&Lücking ***.,*** Moncada,***.&Lücking ***.,*** Moncada,CabreraAmaya&Lücking ***.,*** Moncada,R.-***´rez&***.,*** Lücking,***&Dal Forno ***.,*** Moncada,***´rez-Corredor&Lücking ***.,*** Moncada,***&Lücking ***.,***

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