检索结果-南通市图书馆

Colorimetric detection of glucose and an assay for acetylcholinesterase with amine-terminated polydiacetylene vesicles

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2011年第18期56卷 1877-1883页

作者： XUE WangXin ZHANG DeQing ZHANG guanxin zhu DaoBen Beijing National Laboratory for Molecular Sciences Organic Solids Laboratoo Institute of Chemistry Chinese Academy of Sciences Beijing100190 China

终止胺的 polydiacetylene (PDA ) 的比色的反应泡被改变答案的 pH 开始表明。检测葡萄糖和 acetylcholinesterase (疼痛) 活动的方便比色的方法被利用下列特征成功地用终止胺的 PDA 泡建立：(1 ) 终止胺的 PDA 泡作为答案变化的 pH 经... 详细信息

终止胺的 polydiacetylene (PDA ) 的比色的反应泡被改变答案的 pH 开始表明。检测葡萄糖和 acetylcholinesterase (疼痛) 活动的方便比色的方法被利用下列特征成功地用终止胺的 PDA 泡建立：(1 ) 终止胺的 PDA 泡作为答案变化的 pH 经历比色的转变；(2 ) 葡萄糖能面对葡萄糖 oxidase 被氧化到 gluconic 酸；并且(3 ) 疼痛催化醋胆素的水解作用到醋酸。葡萄糖层次和疼痛活动的视觉察觉显示出好选择和可接受的敏感。葡萄糖的察觉限制是 2.5 mol/L，疼痛活动的水平是象 10.0 mU/mL 一样低的 assayed。而且，终止胺的 PDA 泡能被用于屏蔽禁止者的活动对疼痛。

关键词：乙酰胆碱酯酶葡萄糖胺视觉检测比色法酶测定囊泡聚二乙炔 AChE活性

Preparation and Characterization for Bispecific Antibodies of Anti-CD3×Anti-Idiotype to BCellLym phocytic Leukem ia

在线全文

学校读者我要写书评

暂无评论

Journal of Huazhong University of Science and Technology(Medical Sciences) 1999年第3期19卷 166-169页

作者： SHEN guanxin , ZHANG Yue , zhu Huifen , YANG Jing , WANG Xiaolin Department of Immunology, School of Basic Medical Sciences, Tongji Medical University, Wuhan 430030 Department of Immunology School of Basic Medical Sciences Tongji Medical University Wuhan

Bispecific antibodies (BsAbs) of anti CD3×anti idiotype (Id) to B cell lymphocytic leukemia (CLL) were prepared by chemical conjugation and direct hybridization technique of hybridoma and hybridoma without screening markers. The specificity of BsAbs from culture supernatants or ascites was assayed by indirect ELISA and indirect immunoflurescence (IF). The results showed that BsAbs could specifically react with homologous serum IgM from patients with B CLL and cells carrying CD3 marker respectively. Cell combination test and LDH assay demonstrated that BsAb significantly increased the conjugate formation between lymphocyte activated kill (LAK) cells and Daudi cells, and enhanced the cytotoxic activity of LAK cells against Daudi cells.

关键词： B chronic lymphocytic leukemia bispecific antibody quadruma chemical conjugation

New Molecular Switches and Logic Gates Based Oil Photochromic Spiropyrans

在线全文

学校读者我要写书评

暂无评论

New Molecular Switches and Logic Gates Based Oil Photochromi...

中国化学会第四届有机化学学术会议

作者： Deqing Zhang, Xuefeng Guo, Yucheng Zhou, guanxin Zhang, and Daoben zhu Organic Solids Laboratory, Center for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China

＜正＞It is well known that the reversible structural interconversion between different states of spiropyrans can occur in response to external optical, chemical, and thermal stimulations, accompanied with the substantial changes of their photophysical and photochemical properties. By taking these particular properties of spiropyrans, new combinational logic circuits and even signal communication between molecules have been described. In this presentation, I will describe: （1） modulation of the fluorescence of new dyads with fluorescence and spiropyran units upon light irradiation and the action of ferric ion. Such fluorescence variation mimics the behaviors of logic gates and circuits;（2） "intermolecular signal communication" between spiropyran and 1-aminomethylpyrene/ and a bis-pyrene molecule through proton transfer /and energy transfer in the presence of Zn2+;（3） coordination of the open form of spiropyran with Fe2+ and Fe3+ and the modulation of redox potential of Fe2+/Fe3+. This property of spiropyran was used to photo-control the electron transfer between tetrafulvalene/fluorescein and Fe3+;（4） a new chiral molecular switch based on binaphthanol with two spiropyran units;（5） modulation of the thin film conductivity of polyaniline containing spiropyran.

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

分析化学及实验教学改革

山东化工 2018年第19期47卷 143-144页

作者：孙圣智朱观鑫王大伟山东省邹城市第一中学山东邹城273500 江南大学化学与材料工程学院江苏无锡214122

在分析化学的现状和一些现有的改革提议之上,提出关于分析化学及实验的一些改革建议。通过调整理论课和实验课的比重;本质上改变实验内容、层次;尝试增加实验室的开放;重新考虑教材的选取、编写问题;过程引入对学生的绿色化学意识的教育... 详细信息

在分析化学的现状和一些现有的改革提议之上,提出关于分析化学及实验的一些改革建议。通过调整理论课和实验课的比重;本质上改变实验内容、层次;尝试增加实验室的开放;重新考虑教材的选取、编写问题;过程引入对学生的绿色化学意识的教育等,令学生在学习过程中充分掌握基本知识,进而开展创新尝试。

关键词：分析化学改革绿色化学创新

无动力上肢助力机械外骨骼对手臂抬举作业的影响研究

在线全文

学校读者我要写书评

暂无评论

机械 2023年第8期50卷 16-25页

作者：尹鹏杨靓石磊杨铭朱冠新华南理工大学机械与汽车工程学院广东广州510640 广州视源电子科技股份有限公司博士后工作站广东广州510640

手臂抬举作业是导致上肢工作相关肌肉骨骼疾病(WMSDs)的主要原因,本文的目的是评估无动力上肢助力机械外骨骼(UPAE)在手臂抬举作业中的潜在影响。方法:15名男性志愿者,在三个不同手臂抬举高度(使用和不使用UPAE)下进行重复螺栓安装任务... 详细信息

手臂抬举作业是导致上肢工作相关肌肉骨骼疾病(WMSDs)的主要原因,本文的目的是评估无动力上肢助力机械外骨骼(UPAE)在手臂抬举作业中的潜在影响。方法:15名男性志愿者,在三个不同手臂抬举高度(使用和不使用UPAE)下进行重复螺栓安装任务;测量受试者左、右手臂三角肌前束、中束、斜方肌下束和肱三头肌的肌电图值,并对其颈部、肩部、上臂、前臂、胸背部、腰背部和腿部的感知不适(RPD)进行评分。结果:与不使用UPAE相比,使用UPAE时,右三角肌前束的初始nRMS下降了38.5%,中位nRMS值下降了45.1%,总RPD下降了52.4%。结论:与不使用UPAE相比,使用UPAE可以带来更少的上肢肌肉收缩和更低的RPD,这可能会降低或减慢在手臂抬举作业中上肢WMSDs的水平。

关键词：外骨骼手臂抬举肌肉骨骼疾病肌电图

维普期刊数据库博看期刊

Tetrathiafulvalene (TTF)-based gelators:Stimuli responsive gels and conducting nanostructures

在线全文

学校读者我要写书评

暂无评论

Science China Chemistry 2011年第4期54卷 596-602页

作者： YANG XingYuan ZHANG DeQing ZHANG guanxin zhu DaoBen 1Beijing National Laboratory for Molecular Sciences Organic Solids LaboratoryInstitute of ChemistryChinese Academy of SciencesBeijing 100190China Graduate University of Chinese Academy of Sciences Beijing 100049China

Tetrathiafulvalene (TTF) and its derivatives have been intensively investigated for conducting materials for more than 30 *** π-electron donors,TTF and its derivatives can be reversibly transformed into the respective TTF.+ and TTF2+.Due to its reversible feature,the TTF unit has been widely employed as the building block for switchable *** recent years studies of conducting nanostructures of TTF derivatives have received more and more *** simple way to prepare nano-structures is through the gelation *** this review,we have discussed recent progress in stimuli-responsive gels and conducting nanostructures based on TTF-based gelators.

关键词： tetrathiafulvalene gelator self-assembly gels conducting nanostructures

在线全文

学校读者我要写书评

暂无评论

活性T淋巴细胞亚群双标记测定方法的研究

上海免疫学杂志 1991年第6期11卷 364-367页

作者：代文娟叶维新沈关心苏娜朱惠芬王晓林同济医科大学实验医学研究中心核医学研究室同济医科大学实验医学研究中心免疫室

本文应用抗HLA-DR抗原的单克隆抗体和由OKT系列单抗致敏的血球试剂,建立了一种荧光-花环双标记法同时检测细胞表面双标志的表达。运用这种双标记法对20例正常人外周血表达DR抗原的活性T淋巴细胞亚群进行了测析,并同时与二步分离法进行... 详细信息

本文应用抗HLA-DR抗原的单克隆抗体和由OKT系列单抗致敏的血球试剂,建立了一种荧光-花环双标记法同时检测细胞表面双标志的表达。运用这种双标记法对20例正常人外周血表达DR抗原的活性T淋巴细胞亚群进行了测析,并同时与二步分离法进行了比较,结果表明,二种方法测得的结果无统计学差异。而双标记法具有简便、快速、可靠、不损伤细胞活性、能同时测析细胞表面双抗原和单抗原表达特征等优点,对于一些免疫性疾病的基础与临床研究具有重要的应用价值。

关键词：活性T淋巴细胞亚群双标记测定法 HLA-DR抗原单克隆抗体

Ultrasensitive detection of aliphatic nitro-organics based on“turn-on”fluorescent sensor array

在线全文

学校读者我要写书评

暂无评论

Science China Chemistry 2016年第1期59卷 89-94页

作者： Chen Wang Heng Zhang Li Tian Wei zhu Yue Lan Jian Li Hui Wang guanxin Zhang Deqing Zhang Shiling Yuan Guangtao Li Department of Chemistry Tsinghua UniversityBeijing 100084China Institute of Chemistry Chinese Academy of SciencesBeijing 100190China Department of Chemical Engineering Shandong UniversityJinan 250100China

The broad class of explosives includes nitro aromatics as well as challenging aliphatic nitro-organics whose detection is important from counter-terrorism and national security *** we report a turn-on fluorescent sensor array based on aggregation-induced emission(AIE)fluorophores as *** achieve a good sensing system with fast response,good sensitivity and low detection limit,three receptors with abundant chemical diversities for target analytes were *** turn-on response of the individual receptor showed highly variable and cross-reactive analyte-dependent changes in *** excellent ability to identify a variety of explosives,especially the challenging aliphatic nitro-organics(2,3-dimethyl-2,3-dinitrobutane(DMNB),1,3,5-trinitro-1,3,5-triazinane(RDX),cyclotetramethylene tetranitramine(HMX)and entaerythritol tetranitrate(PETN)),was demonstrated in qualitative and quantitative analyses with 100%*** fluorescence signal amplification in the presence of explosives allows for application of these receptors in a sensor microarray suitable for high-throughput *** results suggested that the cross-reactive sensor array based on AIE fluorophores could find a wide range of applications for sensing various analytes or complex mixtures.

关键词： turn-on fluorescence explosive detection cross-reactive sensory array

IL-10 Gene Modified Dendritic Cells Inhibit T Helper Type 1-Mediated Alloimmune Responses and Promote Immunological Tolerance in Diabetes

在线全文

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2008年第1期5卷 41-46页

作者： Huifen zhu Wenhong Qiu Ping Lei Wei Zhou Xue Wen Fengrong He Li Li Hong Dai guanxin Shen Feili Gong Department of Immunology Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China Medicine and Life Science College of Jianghan University Wuhan 430056 China These authors contributed equally to this work

Dendritic cells （DCs） have the potency to regulate the outcome of autoimmunity through the modulation of immune responses. The induction of antigen specific tolerance is critical for prevention and treatment of allograft rejection. In the present Study, we transfected IL-10 gene into DCs and investigated their effect on inhibition of lymphocyte activity in vitro and induction of immune tolerance on islet allograft in mice. An IDDM C57BL/6 mouse model was induced by streptozotocin. The islet cells isolated from the BALB/c mice were transplanted into the kidney capules of the model mice followed by injection of IL-10 modified DCs （mDCs）. The results showed that mDCs could significantly inhibit T lymphocyte proliferation mediated by allotype cells and induce its apoptosis, whereas, unmodified DCs （umDCs） could promote the murine lymphocyte proliferation markedly. The injection of mDCs could prolong the survival of allotype islet transplanted IDDM mice. The average plasma glucose （PG） level in mDCs treated mice returned to normal within 3 days and lasted for about 2 weeks. The rejection response in control mice occurred for 5 days after transplantation. The level of IFN-γ was lower while IL-4 was higher in mDCs treated mice than that in umDCs treated mice, which indicated that Thl/Th2 deviation occurred. Our studies suggest that IL-10 gene modified DCs can induce the immune tolerance to islet graft and prolong survival of the recipients by the inhibiting of T cell proliferation in allotype mice. Cellular ＆ Molecular Immunology.

关键词： IL-10 dendritic cell IDDM islet transplantation immune tolerance