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Simultaneous nitrification and denitrification conducted by Halomonas venusta MA-ZP17-13 under low temperature conditions

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Acta Oceanologica Sinica 2021年第9期40卷 94-104页

作者： Guizhen Li Qiliang Lai guangshan wei Peisheng Yan Zongze Shao School of Environment Harbin Institute of TechnologyHarbin 150090China Key Laboratory of Marine Genetic Resources Third Institute of OceanographyMinistry of Natural ResourcesXiamen 361005China State Key Laboratory Breeding Base of Marine Genetic Resources Xiamen 361005China Fujian Key Laboratory of Marine Genetic Resources Xiamen 361005China Sourthem Marine Science and Engineering Guangdong Laboratory(Zhuhai) Zhuhai 519080China

Nitrification is a key step in the global nitrogen cycle.Compared with autotrophic nitrification,heterotrophic nitrification remains poorly understood.In this study,Halomonas venusta MA-ZP17-13,isolated from seawater in shrimp aquaculture (Penaeus vannamei),could simultaneously undertake nitrification and denitrification.With the initial ammonium concentration at 100 mg/L,the maximum ammonium-nitrogen removal rate reached98.7%under the optimal conditions including C/N concentration ratio at 5.95,p H at 8.93,and Na Cl at 2.33%.The corresponding average removal rate was 1.37 mg/(L·h)(according to nitrogen) in 3 d at 11.2℃.By whole genome sequencing and analysis,nitrification-and denitrification-related genes were identified,including ammonia monooxygenase,nitrate reductase,nitrite reductase,nitric oxide dioxygenase and nitric oxide synthase;while no gene encoding hydroxylamine oxidase was identified,it implied the existence of a novel nitrification pathway from hydroxylamine to nitrate.These results indicate heterotrophic bacterium H.venusta MA-ZP17-13 can undertake simultaneous nitrification and denitrification at low temperature and has potential for NH_(4)^(+)-N/NH_(3)-N removal in marine aquaculture systems.

关键词： Halomonas heterotrophic nitrification denitrification ammonia oxidization ammonia removal

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鄂尔多斯盆地府谷地区深部煤层气富集成藏规律及有利区评价

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煤田地质与勘探 2024年第2期52卷 81-91页

作者：郭广山徐凤银刘丽芳蔡益栋秦玮陈朝晖邓纪梅李卓伦中海油研究总院非常规研究院北京100028 中国石油学会北京100724 中国地质大学(北京)能源学院北京100083 中海油研究总院钻采研究院北京100028

鄂尔多斯盆地东缘神府区块是典型深部煤层气田,资源丰富且勘探开发潜力巨大。2023年10月,神府深部煤层大气田成功申报探明地质储量超1100亿m^(3)。府谷地区位于神府区块中部,是最早深部煤层气生产试验区,但目前对其煤层气的富集成藏规... 详细信息

鄂尔多斯盆地东缘神府区块是典型深部煤层气田,资源丰富且勘探开发潜力巨大。2023年10月,神府深部煤层大气田成功申报探明地质储量超1100亿m^(3)。府谷地区位于神府区块中部,是最早深部煤层气生产试验区,但目前对其煤层气的富集成藏规律和勘探开发潜力的认识尚不清楚。综合应用地震、测井、钻井和煤岩测试等资料以查明该地区深部煤层气地质特征、富集成藏规律和有利区分布。结果表明:主力煤层4+5、8+9号煤发育稳定且厚度较大(4+5号煤:3.2~5.8 m;8+9号煤:8.7~13.5 m),有利层段主要分布在煤层中上部位置;宏观煤岩类型主要为光亮-半亮煤,煤体结构主要为原生-碎裂结构煤;受深成变质作用的影响,煤类以气煤、肥煤和焦煤为主,煤的变质程度处于热解生气的高峰期,煤储层表现出中-高含气量(4+5号煤:3.0~12.0 m^(3)/t;8+9号煤:7.5~18.5 m^(3)/t)和中-高含气饱和度(35.0%~115.0%)等特征;主力煤层属于低渗储层((0.01~0.09)×10^(-3)μm^(2)),孔隙结构主要以微孔和小孔为主。提出府谷地区煤层气为“挠褶-断层-水动力”富集成藏模式,煤层气富集区位于构造平缓区和斜坡带。建立深部煤层气地质-工程双甜点评价体系,识别出I类地质-工程甜点区位于东部和西南部,是研究区深部煤层气勘探开发首选区带。因此,该研究认识进一步丰富了深部煤层气富集成藏理论,对鄂东缘深部煤层气勘探开发实践具有重要指导意义。

关键词：深部煤层气富集成藏模式有利区府谷地区鄂尔多斯盆地东缘

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基于可视化知识图谱的中医学专业人才培养研究热点与前沿分析

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黑龙江科学 2024年第3期15卷 50-53页

作者：马威莫雪妮郑光珊翟阳广西中医药大学南宁530200 广西国际壮医医院南宁530000

为更好地把握中医专业教育的发展规律,探究其发展轨迹,以中国知网、维普、万方数据库为文献来源,运用CiteSpace 6.1R3软件对2003—2023年我国中医专业人才培养相关研究成果进行可视化分析。研究表明,我国中医专业人才培养工作呈现波动... 详细信息

为更好地把握中医专业教育的发展规律,探究其发展轨迹,以中国知网、维普、万方数据库为文献来源,运用CiteSpace 6.1R3软件对2003—2023年我国中医专业人才培养相关研究成果进行可视化分析。研究表明,我国中医专业人才培养工作呈现波动上升发展态势,新医科是中医专业人才国际化培养的主要研究前沿。中医人才的国际化培养模式应将教育与临床实践相结合,强化新医科建设,培养既适应时代需要又具备全球视野的优秀新医科人才。

关键词：中医学专业人才国际化培养可视化分析

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双路通脑方调节SIRT1/Nrf2/GPx4信号通路对缺血性脑卒中大鼠神经元铁死亡的影响

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医药导报 2024年第4期43卷 526-534页

作者：郑光珊翟阳王凯华马威梅小平陈莹邹敏庞延杨鹏吕艳广西国际壮医医院脑病科南宁530200 广西中医药大学教务处南宁530200 广西国际壮医医院康复科南宁530200 广西国际壮医医院儿科南宁530200 广西中医药大学第一附属医院急诊科南宁530200 广西国际壮医医院科技部南宁530200 广西中医药大学附属瑞康医院脑病科南宁530011

目的探讨双路通脑方对缺血性脑卒中大鼠神经元铁死亡的作用以及对沉默信息调节因子2同源物1(SIRT1)/核因子E2相关因子2(Nrf2)/谷胱甘肽过氧化物酶4(GPx4)信号通路的调节机制。方法采用随机数字表法选取20只大鼠作为假手术组,剩余70只大... 详细信息

目的探讨双路通脑方对缺血性脑卒中大鼠神经元铁死亡的作用以及对沉默信息调节因子2同源物1(SIRT1)/核因子E2相关因子2(Nrf2)/谷胱甘肽过氧化物酶4(GPx4)信号通路的调节机制。方法采用随机数字表法选取20只大鼠作为假手术组,剩余70只大鼠均利用大脑中动脉闭塞法制备缺血性脑卒中大鼠模型,将造模成功的大鼠随机分为模型对照组、双路通脑方组、双路通脑方+SIRT1抑制剂组(双路通脑方+EX527组),每组20只。14 d后,对大鼠进行神经功能损伤评分;TTC染色检测大鼠脑梗死面积;HE染色检测大鼠脑组织病理变化;尼氏染色检测大鼠脑组织神经元数量;试剂盒检测大鼠脑组织中铁离子(Fe^(2+))、超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、丙二醛(MDA)的水平;免疫组化检测大鼠脑组织中酰基-辅酶a合成酶长链家族成员4(ACSL4)、转铁蛋白受体(TFR)、铁蛋白重链多肽1(FTH1)蛋白的阳性表达;Western blotting法检测大鼠脑组织中SIRT1、Nrf2、GPx4及胱氨酸/谷氨酸转运蛋白(SLC7A11)蛋白的表达。结果与假手术组比较,模型对照组大鼠的神经功能缺损评分、脑梗死面积、Fe^(2+)和MDA含量、ACSL4、TFR蛋白表达升高(P<0.05),神经元数量、SOD和GSH含量、FTH1、SIRT1、Nrf2、GPx4及SLC7A11蛋白表达均降低(P<0.05);与模型对照组比较,双路通脑方组大鼠神经功能缺损评分、脑梗死面积、Fe^(2+)和MDA含量、ACSL4、TFR蛋白表达降低(P<0.05),神经元数量、SOD和GSH含量、FTH1、SIRT1、Nrf2、GPx4及SLC7A11蛋白表达均升高(P<0.05);采用SIRT1抑制剂进行回补实验,结果显示SIRT1抑制剂逆转了双路通脑方对神经元铁死亡的抑制作用,同时也抑制了Nrf2和GPx4的表达(P<0.05)。结论双路通脑方可能通过激活SIRT1/Nrf2/GPx4信号通路来抑制缺血性脑卒中大鼠神经元铁死亡。

关键词：双路通脑方缺血性脑卒中神经元铁死亡沉默信息调节因子2同源物1/核因子E2相关因子2/谷胱甘肽过氧化物酶4信号通路

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Preparation of Pickering Emulsion for Antibacterial, Anti-Inflammatory and Wound Healing

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Journal of Biosciences and Medicines 2022年第12期10卷 134-150页

作者： Jinze Pei Ning wei Congcong Cui guangshan Xuan College of Chemical Engineering Qingdao University of Science & Technology Qingdao China

In recent years, natural biodegradable nanoparticles as stabilizers of Pickering emulsions have attracted extensive attention. In this work, a Pickering emulsion composed of chitosan/Arabic gum nanoparticles (CS/GA NPs), tea tree oil and vitamin E was formulated. Then the antibacterial, anti-inflammatory and wound healing abilities of the emulsion were evaluated. Pickering emulsion encapsulated the tea tree oil strengthened antibacterial activity towards Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Candida albicans. Besides, this multi-phase system offered a platform to load with vitamin E, which provides anti-inflammatory effects while antibacterial. Meanwhile, Pickering emulsion avoided contact between bacteria and skin when used in wound treatment.

关键词： Pickering Emulsion CS/GA Nanoparticles Tea Tree Oil Vitamin E Antibacterial Anti-Inflammatory Wound Healing

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Detection of lntraceUular Proteins and Biomarkers Using Modified Silica Nanoparticles and Flow Cytometry In vitro

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Chemical Research in Chinese Universities 2018年第2期34卷 229-234页

作者： YU Yang MA wei YU Lina ZHOU Shuyuan ZHU guangshan SHI wei Department of Sports and Health Guangzhou Institute of Physical Education Guangzhou 510500 P. R. China Key Laboratory for Molecular Enzymology and Engineering Ministry of Education College of Life Science Jilin Universily Changchun 130012 P. R. China China-Japan Union Hospital Jilin University Changchun 130033 P. R. China Key Laboratory of Oral Medicine Guangzhou Institute of Oral Disease Stomatology Hospital of Guangzhou Medical University Guangzhou 510140 P. R. China State Key Laboratory of Inorganic Synthesis and Preparative Chemistry College of Chemistry Jilin University Changchun 130012 R R. China Key Laboratory of Polyoxometalate Science Ministry of Education Faculty of Chemistry Northeast Normal University Changchun 130024 P. R. China

In this paper, antibody-modified silica nanoparticles were successfully synthesized, and their average di- ameter was （1094-9） nm. These particles were mixed with cell extracts to target a protein, then, an antibody labeled with fluorescein isothiocyanate（FITC） was added to form FITC-labeled nanoparticle complexes and the product was analyzed using flow cytometry. The results confirm that the intracellular proteins and biomarkers were precisely and sensitivity detected by the novel method in vitro. The FITC intensity of Akt antibody-conjugated particles was in- creased ≥ 10-fold compared with that of control samples in MCF-7 cells. Furthermore, it can also simultaneously measure several proteins when modified with different antibodies.

关键词： Silica nanoparticle Flow cytometry Intracellular protein Biomarker

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An integrated quantitative proteomics strategy reveals the dual mechanisms of celastrol against acute inflammation

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Chinese Chemical Letters 2021年第7期32卷 2164-2168页

作者： guangshan Xiea Lin Zhu Yuanyuan Song wei Huang Di Hu Zongwei Cai State Key Laboratory of Environmental and Biological Analysis Department of ChemistryHong Kong Baptist UniversityHong KongChina Shenzhen Research Institute and Continuing Education Hong Kong Baptist University.Shenzhen 518000China

Inflammation is a defense mechanism associated with a wide range of diseases.Celastrol is a small molecule isolated from traditional Chinese medicine with potent anti-inflammation activity.In this study,we established an integrated quantitative proteomics strategy to investigate the acute response to celastrol treatment in a rat macrophage cell line challenged with lipopolysaccharide(LPS).Both stableisotopic based non-targeted quantitative profiling and PRM-based targeted quantitation methods were employed.Dimethyl-labeling based non-targeted profiling revealed 28 and 52 proteins that significantly up-and down-regulated by celastrol.Bioinformatics analysis pinpoint key signaling pathways affected.Seven proteins were selected for examining their time-dependent regulatory pattern in response to celastrol using targeted PRM quantitation.The abundance of mRNA at multiple time-points of selected proteins was also examined.Celastrol induced an acute response of selected key transcriptional factors in terms of mRNA or protein abundance within one hour.Interestingly,regulatory trend of mRNA and protein abundance suggested a novel dual mechanism of celastrol in the terms of acute antiinflammation.The integrated quantitative proteomic strategy established in this study constitutes an efficient workflow to characterize key components and their time-dependent regulatory pattern for monitoring drug response.

关键词： Integrated quantitative proteomics Dual mechanisms Celastrol Acute inflammation

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Novel Porous Aromatic Framework with Excellent Separation Capability of CO2 in N2 or CH4

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Chemical Research in Chinese Universities 2014年第6期30卷 1018-1021页

作者： WANG wei YUAN Ye SUN Fuxing ZHAO Ming REN Hao ZHU guangshan Key Laboratory of Automobile Materials Ministry of Education School of Materials Science and Engineering Jilin University Changchun 130022 P. R. China State Key Laboratory of Inorganic Synthesis and Preparative Chemistry College of Chemistry Jilin University Changehun 130012 P. R. China

A novel porous aromatic framework, PAF-52, was obtained via the polymerization of tetrahedral mono- mer tetrakis（4-cyanodiphenyl） methane（TCDPM） with the aid of a facile ionothermal method. PAF-52 has a surface area of 1159 m2/g（BET）, and shows a considerable high separation ability of CO2 in N2 or CH4 respectively at room temperature, using gas-chromatography experiments as evidence,

关键词： Porous aromatic framework CO2 adsorption separation Nitrogen-rich framework

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Porous organic polymers: a progress report in China

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Science China Chemistry 2023年第3期66卷 620-682页

作者： Qing Hao You Tao Xuesong Ding Yajie Yang Jie Feng Rui-Lei Wang Xue-Ming Chen Guan-Le Chen Xiaomeng Li Huang OuYang XunLiang Hu Jia Tian Bao-Hang Han guangshan Zhu wei Wang Fan Zhang Bien Tan Zhan-Ting Li Dong Wang Li-Jun Wan Key Laboratory of Molecular Nanostructure and Nanotechnology Beijing National Laboratory for Molecular SciencesCAS Research/Education Center for Excellence in Molecular SciencesInstitute of ChemistryChinese Academy of SciencesBeijing 100190China CAS Key Laboratory of Nanosystem and Hierarchical Fabrication CAS Center for Excellence in NanoscienceNational Center for Nanoscience and TechnologyBeijing 100190China Key Laboratory of Polyoxometalate and Reticular Material Chemistry of Ministry of Education and Faculty of Chemistry Northeast Normal UniversityChangchun 130024China State Key Laboratory of Applied Organic Chemistry College of Chemistry and Chemical EngineeringLanzhou Magnetic Resonance CenterLanzhou UniversityLanzhou 730000China School of Chemistry and Chemical Engineering State Key Laboratory of Metal Matrix CompositesShanghai Electrochemical Energy Devices Research CenterShanghai Jiao Tong UniversityShanghai 200240China Key Laboratory of Material Chemistry for Energy Conversion and Storage Ministry of EducationSchool of Chemistry and Chemical EngineeringHuazhong University of Science and TechnologyWuhan 430074China Key Laboratory of Synthetic and Self-Assembly Chemistry for Organic Functional Molecules Shanghai Institute of Organic ChemistryChinese Academy of SciencesShanghai 200032China Department of Chemistry Fudan UniversityShanghai 200438China University of Chinese Academy of Sciences Beijing 100049China

Porous organic polymers(POPs) are porous materials composed of light elements such as C, H, N, and O. The benign characters,including large surface area, good physical and chemical stability, well-defined chemical composition, wide ranges of monomer selection, and strong designability, have made POPs one of the frontiers in materials research. In this review, we discussed the design and synthesis of various POP materials that mainly led by Chinese scientists, including conjugated microporous polymers(CMPs), porous aromatic frameworks(PAFs), and hypercrosslinked porous polymers(HCPs), as well as crystalline POPs comprised of covalent organic frameworks(COFs) and a special class of COFs with triazine rings, covalent triazine frameworks(CTFs), and supramolecular organic frameworks(SOFs), and sorted out their main applications in adsorption, separation,catalysis, and electrochemistry fields.

关键词： Porous porous frontier

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Distribution and diversity of bacterioplankton communities in subtropical seawater around Xiamen Island,China

Distribution and diversity of bacterioplankton communities i...

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福建省海洋学会2016年学术年会

作者： Dapeng Shan guangshan wei Mingcong Li Wanpeng Wang Xu Li Zheng Gao Zongze Shao Key Laboratory of Marine Biogenetic Resources Third Institute of OceanographyState Oceanic Administration State Key Laboratory of Crop Biology College of Life SciencesShandong Agricultural University

Marine bacterioplankton communities have profound impact on global biogeochemical cycles and ecological balances.Surveys have been widely performed in seawater of different latitudes,yet relatively little is known about the diversity of bacterioplankton assemblages and the factors shaping their spatial distribution in subtropical island.Here,we investigated the bacterial communities in such a subtropical region,Xiamen Island,using quantitative PCR(qPCR) and 454 pyrosequencing methods.The qPCR results showed that there were higher 16 S rRNA gene abundance in nitrogen and phosphorus-rich sites.Pyrosequencing evidenced that a total of 267 genera of 47 classes in 26 different phyla(or candidate phyla) and some unclassified bacteria were obtained from 52034 high-quality reads.The most dominant phylum was Proteobacteria,followed by Bacteroidetes,Actinobacteria,Firmicutes,Verrucomicrobia and Planctomycetes.Alpha- and Gammaproteobacteria were the most predominant classes,and the most abundant OTU was affiliated with SAR11 clade,followed by Rhodobacteraceae and Oceanospirillaceae.Redundancy analysis(RDA) revealed that total nitrogen and phosphorus were the critical controlling factors to influence the bacterioplankton communities in Xiamen Island.These results will provide insights into bacterial abundance,diversity and distribution patterns,as well as their controlling factors,in subtropical marine ecosystems.

关键词： bacterioplankton diversity pyrosequencing subtropical seawater Xiamen Island environmental factors

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