Simultaneous nitrification and denitrification conducted by Halomonas venusta MA-ZP17-13 under low temperature conditions

作     者：Guizhen Li Qiliang Lai Guangshan Wei Peisheng Yan Zongze Shao Guizhen Li;Qiliang Lai;Guangshan Wei;Peisheng Yan;Zongze Shao

作者机构：School of EnvironmentHarbin Institute of TechnologyHarbin 150090China Key Laboratory of Marine Genetic ResourcesThird Institute of OceanographyMinistry of Natural ResourcesXiamen 361005China State Key Laboratory Breeding Base of Marine Genetic ResourcesXiamen 361005China Fujian Key Laboratory of Marine Genetic ResourcesXiamen 361005China Sourthem Marine Science and Engineering Guangdong Laboratory(Zhuhai)Zhuhai 519080China 

出 版 物：《Acta Oceanologica Sinica》 (海洋学报（英文版）)

年 卷 期：2021年第40卷第9期

页      面：94-104页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 0707[理学-海洋科学] 071005[理学-微生物学] 

基　　金：The COMRA Program under contract No. DY135-B2-01 the Xiamen Ocean Economic Innovation and Development Demonstration Project under contract No. 16PZP001SF16 the National Infrastructure of Natural Resources for Science and Technology Program of China under contract No. NIMR-2017-9。 

主　　题：Halomonas heterotrophic nitrification denitrification ammonia oxidization ammonia removal 

摘      要：Nitrification is a key step in the global nitrogen cycle.Compared with autotrophic nitrification,heterotrophic nitrification remains poorly understood.In this study,Halomonas venusta MA-ZP17-13,isolated from seawater in shrimp aquaculture (Penaeus vannamei),could simultaneously undertake nitrification and denitrification.With the initial ammonium concentration at 100 mg/L,the maximum ammonium-nitrogen removal rate reached98.7%under the optimal conditions including C/N concentration ratio at 5.95,p H at 8.93,and Na Cl at 2.33%.The corresponding average removal rate was 1.37 mg/(L·h)(according to nitrogen) in 3 d at 11.2℃.By whole genome sequencing and analysis,nitrification-and denitrification-related genes were identified,including ammonia monooxygenase,nitrate reductase,nitrite reductase,nitric oxide dioxygenase and nitric oxide synthase;while no gene encoding hydroxylamine oxidase was identified,it implied the existence of a novel nitrification pathway from hydroxylamine to nitrate.These results indicate heterotrophic bacterium H.venusta MA-ZP17-13 can undertake simultaneous nitrification and denitrification at low temperature and has potential for NH_(4)^(+)-N/NH_(3)-N removal in marine aquaculture systems.