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Food Bioscience 2023年第1期51卷 1190-1201页

作者： Yajuan Wang Haoyu Yang guangqing mu Xiaomeng Wu School of Food Science and Technology Dalian Polytechnic UniversityLiaoning116000China

In order to reduce the use of thickening agent in yogurt and increase the viscosity of fermented milk,we screened two probiotics from breast milk as co-cultured with commercial fermenting bacteria in the previous study,and found that the two strains(*** fermentum MWLf-4 and Lactipiantibacillus plantarum MWLp-12)had a great ability to increase the viscosity of fermented *** that,in this paper we observed the extracellular polysaccharides of single strain fermented milk using inverted fluorescence ***,the complete gene sequences and safety evaluation including antibiotic sensitivity test,hemolysis test and determination of amine in vitro of Limosilactobacillus fermentum MWLf-4 and Lactipiantibacillus plantarum MWLp-12 were carried *** a conclusion,the two strains possess a better ability to produce extracellular polysaccharide,and they are potentially safe *** is worth noting that the genes that produce exopolysaccharides of tagE and glmu are found in Limosilactobacillus fermentum MWLf-4,and cps gene cluster and galE are found in Lactipiantibacillus plantarum ***,this study provides a reliable theoretical basis for further elaborating the relationship between genes and functionality.

关键词： Human milk source probiotics Complete genome sequence Safety evaluation Extracellular polysaccharide

Influence of soaking Malus domeri(Bois)*** on gut microbiota and metabolites of long-living elderly individuals in Hezhou city,Guangxi,China

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Food Science and Human Wellness 2024年第3期13卷 1311-1321页

作者： Hui Nie Zhongyang Gao Yanghe Luo Yajuan Wang Feiyang Wu guangqing mu Xiaomeng Wu School of Food Science and Technology Dalian Polytechnic UniversityDalian 116034China Dalian Probiotic Function Research Key Laboratory Dalian Polytechnic UniversityDalian 116034China Guangxi Key Laboratory of Health Care Food Science and Technology Hezhou UniversityHezhou 542899China

To investigate the effects of drinking the soaking of Malus domeri(Bois)*** on gut microbiota and metabolites of long-living elderly individuals in Hezhou city,Guangxi,*** has been reported that longevity is closely related to metabolism and the gut *** 16S rRNA sequencing and liquid chromatography-mass spectrometry(LC-MS)were used to analysis fecal samples and explore the factors affecting longevity in the ***,we discovered,that elderly individuals who had been drinking the soaking of ***(Bois)*** for a long time exhibited higher diversity of the gut microbiota than without drinking the soaking,*** proportions of Ruminococcaceae and Prevotella were decreased in those who did not drink this *** addition,a total of 106 metabolites were characterized,and the people of long-lived people(>90 years old)and elderly people(<90 years old)who drinking soaking of ***(Bois)*** significantly altered the gut microbiota and upregulated levels of haplopine,farnesol,genipic acid,momordicinin,2-hydroxyestrone,hydroxyphenyllactic acid,caffeic acid,sophoraflavanone B,and soyasaponin *** preliminarily determined that ***(Bois)*** consumption may be an important factor affecting longevity in this area.

关键词： Longevity Malus domeri(Bois)Chev.leaves Gut microbiota Metabolomics

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Proteomics analysis of the hypothalamus of high-fat diet fed mice after Lactiplantibacillus plantarum Y44 administration

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Food Bioscience 2022年第3期47卷 1166-1176页

作者： Xiaomeng Wu Yajuan Wang Xinyu Du Yujun Liu Yuan Gao Yanfeng Tuo guangqing mu School of Food Science and Technology Dalian Polytechnic UniversityLiaoning116000People's Republic of China Food Science Institute Zhejiang Academy of Agricultural SciencesHangzhou310021People's Republic of China

Probiotics could regulate human health via changing gut *** previous studies found that Lactiplantibacillus plantarum Y44 (*** Y44) was shown to reverse obesity-related symptoms of high-fat diet fed mice by influencing gut microbiota,which ameliorated lipid metabolism disorders and intestinal *** this study,the effects of *** Y44 on high-fat fed mice and the involved mechanisms were investigated through hypothalamus proteomics analysis,which revealed 22 downregulated and 21 upregulated *** expressed proteins between *** Y44 intervention group and high-fat fed group in hypothalamus of mice involved in metabolic pathways were concentrated in endocrine regulation of carbohydrate,amino acid and energy generation *** hypothalamus proteomics results about the influences on most of differentially expressed proteins of *** Y44 administering high-fat fed mice has been ***,*** Y44 would be a potential functional strain for the amelioration of high-fat diet associated health problems.

关键词： High-fat diet Lactiplantibacillus plantarum Proteomic

In vitro antibacterial activity of Bacillus coagulans T242 on Caco-2 cells infected with Salmonella Typhimurium

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Food Bioscience 2023年第2期52卷 1369-1378页

作者： Xiaoxi Gao Jie Yu Lixuan Chang Yunchao Wang Xinping Sun guangqing mu Fang Qian School of Food Science and Technology Dalian Polytechnic UniversityDalian116034PR China

This study aimed to investigate the antibacterial activity of Bacillus coagulans(***)T242 and its ability to alleviate the infection of Caco-2 cells with Salmonella Typhimurium(***)*** T242 has a broad spectrum of the antibacterial *** cell-free supernatants of *** T242 inhibited the growth of *** SL1344 and changed its cell *** T242 inhibited the adhesion and invasion of *** SL1344 to Caco-2 cells by adhering to Caco-2 cells,significantly reduced the production of interleukin-6(IL-6),Interleukin-8(IL-8)and tumor necrosis factor-α(TNF-α)which induced by *** SL1344,also increased interleukin-10(IL-10)production,thereby alleviating the inflammatory response of Caco-2 *** T242 vegetative cells and spores could alleviate the decreased expression of tight junction proteins(Claudin-1,Occludin and ZO-1)caused by *** SL1344 infection in Caco-2 cells by increasing the expression of Claudin-1,Occludin and *** study demonstrated that *** T242 could protect Caco-2 cells by adhesion,reducing inflammatory response and enhancing intestinal barrier ***,*** T242 will hopefully be an effective alternative to antibiotics,and this study has important implications for food safety issues.

关键词： Bacillus coagulans Antibacterial Salmonella Typhimurium Inflammatory

Probiotic properties of Lactobacillus paraplantarum LS-5 and its effect on antioxidant activity of fermented sauerkraut

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Food Bioscience 2023年第2期52卷 1303-1313页

作者： Hongchi Zhou Sixuan Wang Weichao Liu Lixuan Chang Xuemei Zhu guangqing mu Fang Qian School of Food Science and Technology Dalian Polytechnic UniversityLiaoning116000China

This study aimed to investigate the safety and probiotic properties of Lactobacillus paraplantarum LS-5 isolated from traditional northeastern sauerkraut,and to assess the antioxidant capacity of different solvent extracts(ethanol,water,and hot water)of *** LS-5-inoculated *** LS-5 was shown to have various probiotic characteristics,such as good survival ability under artificial gastrointestinal conditions,antagonistic activity of various pathogens,non-biogenic amines,antibiotic susceptibility,and *** 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonate(ABTS)radical scavenging activity of the *** LS-5-inoculated sauerkraut alcohol extract(71.13%)was significantly higher than water extract(59.59%)(p<0.05).The ferric reducing antioxidant power(FRAP)of sauerkraut alcohol extract(614.93 Troloxμmol L^(-1))exhibited a slightly stronger ability compared to water extract(388.53 Troloxμmol L^(-1))and the heated sauerkraut water extract(383.20 Troloxμmol L^(-1))at extract concentrations of 5.0 mg mL^(-1).Its 1,1-diphenyl1-2-picrylhydrazyl(DPPH)radical scavenging activity was also *** was found by principal component analysis(PCA)and Pearson that the DPPH radical-scavenging activities of sauerkraut extracts were significantly correlated with the content of polyphenols and flavonoids(p<0.05).Unlike kimchi,Northeastern sauerkraut is consumed mainly by heating,so this study could contribute to understanding the antioxidant capacity of different solvent extracts of sauerkraut,and before and after heating water extracts.

关键词： Lactobacillus paraplantarum Probiotic properties Fermentation vegetable Radical-scavenging

Improvement of Lactiplantibacillus plantarum MWFLp-182 on oxidative deficits induced by in 2，2'-azobis(2-methylpropionamidine) dihydrochloride and the relating key gene analysis

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Food Science and Human Wellness 2024年

作者： Hui Nie Xutong Ma Fanyu Kong Yanghe Luo guangqing mu Xiaomeng Wu School of Food Science and Technology， Dalian Polytechnic University Dalian Probiotic Function Research Key Laboratory， Dalian Polytechnic University Guangxi Key Laboratory of Health Care Food Science and Technology， Hezhou University

Lactiplantibacillus plantarum MWFLp-182 was originally isolated from the feces of long-living elderly individuals in Hezhou city， Guangxi， China. L. plantarum MWFLp-182 showed favorable gastric and intestinal tolerance compared with Lactiplantibacillus rhamnosus GG (LGG). L. plantarum MWFLp-182 was further examined for its antioxidant and anti-inflammatory activity in ABAP-treated HT-29 cells. Importantly， it enhanced the expression of anti-inflammatory factor (interleukin-10 (IL-10))， antioxidant cytokines (superoxide dismutase (SOD)， glutathione peroxidase (GPx)， nuclear factor erythroid 2-related factor (Nrf2)， and hemeoxygenase 1(HO-1))， B-cell lymphoma-2 (Bcl-2)， and tight junction (Zonula occludens protein 1(ZO-1)， Claudin-1， and Occludin) proteins and genes， and reduced reactive oxygen species (ROS). The complete genome of L. plantarum MWFLp-182 contained a single circular chromosome that was 3 257 196 bp long， with a G + C content of 44.49%， and a single circular plasmid that was 53 560 bp long. The genes tpx， trxA， trxB， npr， nrdH， dps， recA， gpx， gshA and arsC in this strain were related to antioxidant function; the key genes involved in antioxidant function were further investigated. In this study， we identified a probiotic candidate with antioxidant properties.

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Lactiplantibacillus plantarum DPUL-S164 regulate AhR signaling to ameliorate DSS-induced intestinal barrier damage by producing indole-3-lactic acid in a tryptophan-rich diet

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Food Science and Human Wellness 2024年

作者： Arong Wang Dashuai He Tieqi Wang Cheng Guan guangqing mu Yanfeng Tuo School of Food Science and Technology， Dalian Polytechnic University Dalian Probiotics Function Research Key Laboratory， Dalian Polytechnic University

The aim of this paper was to study the effect of combination of Lactobacillus strains and tryptophan-rich diet on the intestinal barrier function of Balb/c mice exposed to a cocktail of antibiotics (ABX)and dextran sodium sulfate (DSS). Several Lactobacillus strains isolated from the healthy human fecal sample was found to utilize tryptophan(Trp) to produce indole derivatives. The results of Trp metabolism indicated that the ability of Lactobacillus to metabolize Trp to produce Indole-3-lactic Acid (ILA)， indole-3-carboxaldehyde (I3A)， and indole-3-acetic acid (IAA)varies in vitro and in vivo. The effect of Lactobacillus with high-yielding indole derivatives on disease activity index (DAI)， colon length， and intestinal permeability was significantly better than that of Lactobacillus with low-yielding indole derivatives in a high Trp diet. And Lactobacillus combined with Trp intervention also had a certain regulatory effect on the intestinal flora of male Balb/C mice. Among them， Lactiplantibacillus plantarum (L. plantarum) DPUL-S164 produced more ILA both in vivo and in vitro， and the combination of L. plantarum DPUL-S164 and Trp significantly decreased the expression level of the serum pro-inflammatory cytokine interleukin (IL)-6 and increased the expression level of the anti-inflammatory cytokine IL-10， significantly improved the number of goblet cells in the mouse mucous layer and increased mucin and tight junction protein expression. Furthermore， L. plantarum DPUL-S164 combined with Trp intervention activated the aryl hydrocarbon receptors (AhR) signaling pathway. Furthermore， we found that the expression of colonic tight junction protein was positively correlated with the expression of colonic AhR， and the expression of AhR was positively correlated with the concentrations of ILA and I3A in vivo. Therefore， we conclude that the ILA as AhR ligand produced by L. plantarum DPUL-S164 regulated the AhR pathway， thus up-regulating the expression of the tight junction

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Synbiotic AHY improves hyperlipidaemia in HFD-fed mice by activating gut-liver axis through modulation of the gut microbiota

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Food Science and Human Wellness 2024年

作者： Lai Jiang Xueqi Lu Xudong Lin Zhiqi Cao Ruonan Liu guangqing mu Shujuan Jiang School of Food Science and Technology， Dalian Polytechnic University

In our previous study， a synbiotic AHY could improve constipation was developed using three probiotic strains (Lactiplantibacillus plantarum Y12， AHQ-14 and HM22) and four prebiotics (stachyose， fructooligosaccharides， isomaltooligosaccharide and galactooligosaccharides). The in vitro cholesterol-scavenging capacity of three probiotic strains was assessed， revealing that AHY may possess lipid-lowering potential. Consequently， the in vivo lipid-lowering efficacy of AHY was evaluated by establishing a hyperlipidemia model using HFD (high-fat diet)-fed C57/6J mice and administering AHY over a period of 12 weeks. Various parameters including biochemical indexes， histological morphology， metabolites， gene expression and intestinal flora were analyzed to evaluate the hypolipidemic effect of AHY in vivo. The results demonstrated that gavage administration of AHY led to a significant reduction in body weight and organ indexes， as well as an improvement in lipid abnormalities in serum. Moreover， AHY significantly reduced serum TBAs (total bile acids) and significantly increased fecal TBAs. Furthermore， AHY up-regulated tight junction proteins (claudin-1， ZO-1， occludin) and down-regulated inflammatory factors (IL-6， IL-1β， TNF-α)， enhancing intestinal barrier function and reducing colon inflammation. Additionally， AHY influenced the composition of gut microbiota by increasing beneficial bacteria (Lactobacillus， Bifidobacterium) and decreasing harmful bacteria (norank＿＿f＿＿ Desulfovibrionaceae，norank＿＿f＿＿Oscillospiraceae)， resulting in higher level of SCFAs (short-chain fatty acids) in the gut. This modulation of bacterial flora contributed to the regulation of cholesterol metabolism through activating the gut-liver axis pathway， effectively reducing blood lipid levels. These findings suggest that AHY have the potential to be a functional synbiotic for alleviating hyperlipidemia and offers novel insights to understand the mechanism for probiotic products alleviating hyperlipid

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大豆油生产中生物及化学性危害的分析和控制

中国油脂 2010年第3期35卷 14-17页

作者：藏小丹牟光庆大连工业大学生物与食品工程学院辽宁大连116034

通过对原料大豆及其加工、储运过程中的生物及化学危害分析,找出了危害大豆油食用安全的主要原因。在原料大豆储运过程中,应严格控制大豆在安全水分(13.5%)以下;在大豆油的加工过程中,应严格控制溶剂残留和防止大豆油的氧化酸败,这样可... 详细信息

通过对原料大豆及其加工、储运过程中的生物及化学危害分析,找出了危害大豆油食用安全的主要原因。在原料大豆储运过程中,应严格控制大豆在安全水分(13.5%)以下;在大豆油的加工过程中,应严格控制溶剂残留和防止大豆油的氧化酸败,这样可有效地消除安全隐患,保证食用安全。

关键词：大豆油危害控制

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高效液相色谱-串联质谱法测定饲料和卧床土中的环境雌激素

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色谱 2013年第9期31卷 908-913页

作者：李雪牟光庆陈历俊姜铁民大连工业大学食品学院辽宁大连116034 北京三元食品股份有限公司北京100076

建立了饲料和卧床土中雌三醇、雌二醇、雌酮、双酚A和己烯雌酚5种环境雌激素的固相萃取结合高效液相色谱-串联质谱(HPLC-MS/MS)测定方法。对色谱流动相、质谱条件、固相萃取柱等影响因素进行了优化,得到的最优化条件为:样品经乙腈提取后... 详细信息

建立了饲料和卧床土中雌三醇、雌二醇、雌酮、双酚A和己烯雌酚5种环境雌激素的固相萃取结合高效液相色谱-串联质谱(HPLC-MS/MS)测定方法。对色谱流动相、质谱条件、固相萃取柱等影响因素进行了优化,得到的最优化条件为:样品经乙腈提取后,用固相萃取柱(NH2-SPE)进行富集,采用Acquity UPLCTM HSS T3色谱柱分离,以乙腈-甲醇(4∶1,v/v)与0.01%氨水为流动相,采用梯度洗脱,在负离子模式下进行MS/MS测定。在该优化条件下,5种环境雌激素的检出限(以信噪比为3计)为0.06～0.22μg/kg,回收率为81.70%～102.20%,相对标准偏差小于10.00%。该方法用于饲料和卧床土中的5种环境雌激素残留量的测定具有简便、快速、灵敏的特点。

关键词：高效液相色谱-串联质谱环境雌激素饲料卧床土

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