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Acclimatory responses to high-salt stress in Chlamydomonas (Chlorophyta, Chlorophyceae) from Antarctica

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Acta Oceanologica Sinica 2012年第1期31卷 116-124页

作者： kan guangfeng SHI Cuijuan WANG Xiaofei XIE Qiuju WANG Min WANG Xinlei MIAO Jinlai School of Marine Science and Technology Harbin Institute of Technology at WeihaiWeihai 264209China Key Laboratory of Marine Bio-active Substances State Oceanic AdministrationQingdao 266061China

Antarctic ice microalga can survive and thrive in channels or pores containing high salinity in Antarctic ice layer. In this study, it was found that cell membrane permeability of green microalga Chlaraydomonas sp. L4 from Antarctic sea ice was high in cells treated with hypersalinity due to the induction of active oxygen and radicals. However, increased super oxide dismutase （SOD） scavenged harmful free radicals effectively to keep cell membrane integrity. Also, the analysis of membrane fatty acids demonstrated the content of saturated fatty acids and monounsaturated fatty acids in- creased and polyunsaturated fatty acids decreased under the high-salt treatment for 14 d, which effectively reduced the membrane fluidity and minimized the injury to cell membrane. The morpho- logical changes showed that hypersalinity induced the increase of cell volume and the consumption of starch granules. However, because of the increase in detoxification of vacuoles, electron-dense deposits and SOD activity under high-salt stress, the complete noninterference thylakoids, mito- chondria and cell nucleus maintained cellular fundamental metabolism. Global-expression profiling of proteins showed eight protein spots disappeared, 18 protein spots decreased and 18 protein spots were enhanced after the high-salt shock obviously （P 〈0.05）. One new peptide （pI 6.90; MW 51 kDa） was primarily confirmed as the processor of light reaction center protein CP43 in photosystem II, which increased photosynthesis ability of Chlamydomonas sp. L4 treated with high salinity.

关键词： Antarctic ice microalga SOD enzymes ultra structure membrane fatty acids difference protein hypersaline acclimation

Temperature-dependent Raman spectroscopy studies of 1-5-layer WSe2

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Nano Research 2020年第2期13卷 591-595页

作者： Zhonglin Li Yingying Wang Jie Jiang Yao Liang Bo Zhong Hong Zhang Kai Yu guangfeng kan Mingqiang Zou Department of Optoelectronic Science Harbin Institute of Technology at WeihaiWeihai 264209China School of Marine Science and Techmology Harbin Institute of Technology at WeihaiWeihai 264209China School of Materials Science and Engineering Dalian Jiaotong University Dalian 116028China School of Materials Science and Engineering Harbin Institute of Technology at WeihaiWeihai 264209China Chinese Academy of Inspection and Quarantine(CAIQ) No.A3Gaobeidian RoadChaoyang DistrictBeijing 100123China

In this work,Raman measurements of 1-5-layer WSe2 supported on SiO2/Si in the temperature range of 133 to 533 K are reported.A physical model including both volume effect and temperature effect is used to quantitatively understand the nonlinear temperature dependence of E2g^1Raman *** is found this nonlinear dependence of Raman mode mainly originates from thermal expansion effect and three-phonon *** former effect increases with an increase in number of layers,which is inverse for the latter *** temperature-dependent thermal expansion coefficients of 1-5-layer WSe2 are also obtained from Raman *** full width at half maximum(FWHM)of E;g mode is also systematically studied both experimentally and theoretically in the temperature range of 133 to 413 *** is found that the increase in FWHM of E2g^1 mode originates from decaying of E2g^1 *** work will promote the understanding of anharmonic behaviors of phonons in WSe2 flakes with different thicknesses.

关键词： WSe2 thermal expansion coefficients temperature-dependent Raman spectroscopy

南极冰藻Chlamydomonas sp.L4的UV-B辐射效应研究

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高技术通讯 2008年第9期18卷 966-970页

作者：阚光锋史翠娟缪锦来李光友中国海洋大学海洋生命学院青岛266003 哈尔滨工业大学(威海)海洋学院威海264209 国家海洋局海洋生物活性物质重点实验室青岛266061

实验研究了从南极采集的冰藻 Chlamydomonas sp.L4经强 UV-B 辐射胁迫后的生物学效应及逆境适应性。研究显示,经过强 UV-B 胁迫,冰藻 L4细胞内迅速产生大量的活性氧,丙二醛含量升高;同时,SOD 活性快速提高,使活性氧和丙二醛含量维持在... 详细信息

实验研究了从南极采集的冰藻 Chlamydomonas sp.L4经强 UV-B 辐射胁迫后的生物学效应及逆境适应性。研究显示,经过强 UV-B 胁迫,冰藻 L4细胞内迅速产生大量的活性氧,丙二醛含量升高;同时,SOD 活性快速提高,使活性氧和丙二醛含量维持在细胞容许范围之内。膜脂肪酸成分分析发现不饱和指数增加了近4倍,膜流动性增加,从而维持膜的正常功能。光学显微镜、扫描和透射电镜观察表明,UV-B 辐射使细胞体积显著增大,黏性多糖分泌增多,细胞壁增厚,未知黑色颗粒密度增加,L4可能通过上述结构变化把 UV-B 屏蔽在胞外。脂肪颗粒的增加是细胞在逆境生存的能量保障。此外,细胞中类囊体片层、线粒体和细胞核等结构基本没有变化,维持细胞基本的代谢和能量供应。

关键词：南极冰藻 UV-B辐射膜脂过氧化膜脂肪酸形态结构逆境适应性

解析电喷雾电离质谱成像技术在植物学研究中的应用

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分析试验室 2021年第5期40卷 613-620页

作者：周司涵史翠娟姜杰刘吉林张洪阚光锋哈尔滨工业大学(威海)海洋科学与技术学院威海264209 哈尔滨工业大学化工和化学学院哈尔滨150001 哈尔滨工业大学城市水资源与水环境国家重点实验室哈尔滨150090

解析电喷雾电离质谱成像(DESI-MSI)是一种常用的质谱成像方法,可在常压下将待测样品引入质谱仪进行检测,并用成像软件构建出被检物质的空间离子分布图像。近年来,DESI-MSI因其常压操作、碎片离子少以及高通量等优势而被广泛应用于医药... 详细信息

解析电喷雾电离质谱成像(DESI-MSI)是一种常用的质谱成像方法,可在常压下将待测样品引入质谱仪进行检测,并用成像软件构建出被检物质的空间离子分布图像。近年来,DESI-MSI因其常压操作、碎片离子少以及高通量等优势而被广泛应用于医药、工业、食品、生物学等领域。本文综述了近年来DESI-MSI在植物学研究中的应用,包括药用植物药用成分鉴定、粮食作物及经济作物中成分的分布情况。最后,概述了DESI-MSI技术在植物学研究中存在的主要问题和解决办法,并对发展前景进行了展望。

关键词：质谱成像 DESI 药用植物粮食作物经济作物应用进展

南极红酵母AN5的发酵及对海参体成分的影响

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极地研究 2018年第1期30卷 50-56页

作者：王晓飞史翠娟阚光锋李亮文华周婷哈尔滨工业大学(威海)功能食品研究室山东威海264209 山东大学(威海)海洋学院山东威海264209

红酵母是海洋酵母中的常见种类,在水产养殖中常作为微生态制剂。本实验以第22次南极考察从海冰中分离的红酵母AN5为研究对象,对酵母菌的发酵生长条件进行了优化,结果表明,红酵母培养的最佳碳源和氮源分别为2%的糖蜜和0.5%的酵母粉,最适... 详细信息

红酵母是海洋酵母中的常见种类,在水产养殖中常作为微生态制剂。本实验以第22次南极考察从海冰中分离的红酵母AN5为研究对象,对酵母菌的发酵生长条件进行了优化,结果表明,红酵母培养的最佳碳源和氮源分别为2%的糖蜜和0.5%的酵母粉,最适装液量为50 m L/250 mL,摇瓶转速为140 r·min^(–1),培养基初始pH=3.0,20℃下可获得最佳培养,此时酵母发酵密度可达5.81×10~7 cells·mL^(–1)。发酵后测得酵母中水分含量为69.15%,干酵母蛋白质含量为42.00%,多糖含量为32.08%,脂肪含量为0.39%。在幼参养殖过程中以0.3 g(湿重)·m^(–3)水体的用量投喂极地红酵母,养殖2个月后,与对照相比,幼参体内水分和脂肪含量基本没有变化,而蛋白质和多糖含量明显升高。因此,极地红酵母可大量发酵生产,作为微生态制剂应用于海参的养殖中。

关键词：极地红酵母培养条件幼参微生态制剂

Foundation of protein preparation method and 2-DE analyses of anti-radiation proteins in Antarctic ice microalga Chlamydomonas sp.

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Foundation of protein preparation method and 2-DE analyses o...

Study on proteins and the resistance to high salinity in Antarctic ice microalgae Chlamydomonas sp.

中国海洋学会海洋生物工程2005年学术年会

作者： kan guangfeng MIAO jinlai DING Yu LI Guangyou Marine life college, Ocean University of China, Qingdao 266003, China Key laboratory of marine bio - active substances, SOA. , Qingdao 266061, China

Protein preparation is the key step of two - dimensional gel electrophoresis. Antarctic ice microalga Chlamydomonas sp. was used as material in our experiments, from which protein extract methods were compared, and the results showed that improved Trichloroacetic acid (TCA) -acetone fractional precipitation method was one of the fittest methods to protein extraction from Antarctic ice algae. At the same time, we founded a complete 2 - D electrophoresis system fit to Antarctic ice alga proteins. We also analyzed the anti - radiation proteins in Chlamydomonas sp. by 2 - D gel electrophoresis, and two new peptides (21 and 23 kD) were found after exposing to UV radiation.

关键词： Antarctic ice alga Chlamydomonas sp. resoluble proteins 2 - D PAGE UV - B radiation

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Study on proteins and the resistance to high salinity in Ant...

一株南极细菌(Pseudoalteromonas sp.)对模拟海水养殖水体的净化作用

中国海洋学会海洋生物工程2005年学术年会

作者： kan guangfeng MIAO Jinlai ZHENG Zhou LI Guangyou Life Sciences and Technology College, Ocean University of China, Qingdao 266003, China. Key Laboratory of Marine Bio - active Substances, SOA, Qingdao 266061, China

Antarctic ice microalga Chlamydomonas sp. can survive and thrive in channels or tubs containing high salinity in Antarctic ice layer. The comparison of growth in different salinity showed that Chlamydomonas sp. could survived in the salinity of 132‰. Survival in extreme conditions requires a complex suite of physiological and metabolic adaptations. However, it is not known what initial defense mechanisms in protein level occur in response to high salinity. In this study, global - expression profiling of proteins in response to high - salinity stress (99‰) was analyzed by SDS - PAGE, two - dimensional gel electrophoresis and image analysis. SDS - PAGE analysis showed that the density of 2 bands (26 and 36 kD) decreased obviously. The soluble proteins in Chlamydomonas sp. were compared by 2 - D gel electrophoresis, and the synthesis of 8 protein spots was found disappeared and 18 decreased after the shock of high salinity. In addition, 19 protein spots were enhanced or induced, among which only one new peptides (51 kD) appeared. They might be correlated with the resistance to high salinity.

关键词： Antarctic ice alga Chlamydomonas sp. resoluble proteins 2 - D PAGE analysis high - salinity press

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极地研究 2010年第3期22卷 313-320页

作者：迟明磊阚光锋史翠娟路来风林学政刘胜浩海洋学院威海264209 国家海洋局海洋生物活性物质重点实验室青岛266061

试验选用一株高产低温蛋白酶的南极细菌菌株Pseudoalteromonas ***64,从溶解氧、化学需氧量、氨氮、亚硝态氮、pH这五个方面研究了南极细菌对养殖水体的影响。结果显示,随着饲料中蛋白质的散失,水体中蛋白质的含量在第7天达到高峰,溶解... 详细信息

试验选用一株高产低温蛋白酶的南极细菌菌株Pseudoalteromonas ***64,从溶解氧、化学需氧量、氨氮、亚硝态氮、pH这五个方面研究了南极细菌对养殖水体的影响。结果显示,随着饲料中蛋白质的散失,水体中蛋白质的含量在第7天达到高峰,溶解氧下降,化学需氧量持续上升,在第11天达最大值9.88mg/L,并且维持在较高的水平。缺氧条件下,有害物质氨氮和亚硝态氮含量逐渐增加,在第9天和第11天最大值分别达0.65mg/L和0.352mg/L,而pH持续下降,在第9天达最低值5.95。当水体中加入高产蛋白酶的南极菌株AN64后,蛋白质含量在第5天即达高峰,溶解氧无明显变化,化学需氧量在第7天达最高峰,比对照组提前4天,但含量仅约为后者的一半。同时,氨氮和亚硝态氮含量远低于对照组,分别仅为对照的47.7%和26.5%,而pH有小幅下降,维持在6.85到7.52之间。由此可以看出,南极菌株产生的低温蛋白酶可以有效分解海水中的蛋白质,降低水体中的氨氮和亚硝态氮等有害物质,维持pH的稳定,为健康养殖提供了有效途径。

关键词：南极细菌低温蛋白酶海水养殖水质净化

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质谱成像技术在海洋生物中的应用进展

海洋通报 2023年第2期42卷 216-231页

作者：阚光锋焦茜张洪于凯姜延晓王英英程紫逸姜杰哈尔滨工业大学(威海)海洋科学与技术学院山东威海264209 哈尔滨工业大学化工和化学学院黑龙江哈尔滨150001 哈尔滨工业大学城市水资源与水环境国家重点实验室黑龙江哈尔滨150090 哈尔滨工业大学(威海)理学院山东威海264209

质谱成像是一种以质谱分析为基础的新兴分子影像技术,无须复杂的前处理过程即可对样品中多种被测分子的空间分布进行检测。目前,该技术已被广泛应用于医学、药学、微生物学、食品科学等诸多领域,其在海洋生物领域的应用也逐渐受到关注,... 详细信息

质谱成像是一种以质谱分析为基础的新兴分子影像技术,无须复杂的前处理过程即可对样品中多种被测分子的空间分布进行检测。目前,该技术已被广泛应用于医学、药学、微生物学、食品科学等诸多领域,其在海洋生物领域的应用也逐渐受到关注,为海洋科学基础研究提供了新机遇。本文在对不同质谱成像技术原理和特点进行简述的基础上,从海洋生物所产天然产物、关键代谢元素与体内污染物监测等方面,对近年来质谱成像技术在海洋生物中的研究应用进行了系统综述。

关键词：质谱成像离子化技术海洋生物应用进展