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LncRNA HIF1A-AS2 accelerates malignant phenotypes of renal carcinoma by modulating miR-30a-5p/SOX4 axis as a ceRNA

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Cancer Biology & Medicine 2021年第2期18卷 587-603页

作者： Mingwei Chen Xuedong Wei Xiu Shi Le Lu guangbo zhang Yuhua Huang Jianquan Hou Department of Urology The First Affiliated Hospital of Soochow UniversitySuzhou 215006China Department of Obstetrics and Gynecology The First Affiliated Hospital of Soochow UniversitySuzhou 215006China Jiangsu Institute of Clinical Immunology The First Affiliated Hospital of Soochow UniversityJiangsu Key Laboratory of Clinical ImmunologySoochow UniversityJiangsu Key Laboratory of Gastrointestinal Tumor ImmunologySuzhou 215006China

Objective:Several reports have proposed that lnc RNAs,as potential biomarkers,participate in the progression and growth of malignant ***1 A-AS2 is a novel lnc RNA and potential biomarker,involved in the genesis and development of ***,the molecular mechanism of HIF1 A-AS2 in renal carcinoma is ***:The relative expression levels of HIF1 A-AS2 and miR-30 a-5 p were detected using RT-qPCR in renal carcinoma tissues and cell *** loss-of-function and overexpression,the biological effects of HIF1 A-AS2 and miR-30 a-5 p in kidney carcinoma progression were *** luciferase reporter gene analysis and Western blot were used to detect the potential mechanism of HIF1 A-AS2 in renal ***:HIF1 A-AS2 was upregulated in kidney carcinoma tissues when compared with para-carcinoma tissues(P<0.05).In addition,tumor size,tumor node mestastasis stage and differentiation were identified as being closely associated with HIF1 A-AS2 expression(P<0.05).Knockdown or overexpression of HIF1 A-AS2 either restrained or promoted the malignant phenotype and WNT/β-catenin signaling in renal carcinoma cells(P<0.05).Mi R-30 a-5 p was downregulated in renal cancers and partially reversed HIF1 A-AS2 functions in malignant renal tumor ***1 A-AS2 acted as a micro RNA sponge that actively regulated the relative expression of SOX4 in sponging miR-30 a-5 p and subsequently increased the malignant phenotypes of renal ***1 A-AS2 showed a carcinogenic effect and miR-30 a-5 p acted as an antagonist of the anti-oncogene effects in the pathogenesis of renal ***:The HIF1 A-AS2-miR-30 a-5 p-SOX4 axis was associated with the malignant progression and development of renal *** relative expression of HIF1 A-AS2 was negatively correlated with the expression of miR-30 a-5 p,and was closely correlated with SOX4 mRNA levels in renal cancers.

关键词： HIF1A-AS2 SOX4 miR-30a-5p kidney carcinoma ceRNA

A Novel Subset of B7-H3+CD14+HLA-DR-/low Myeloid-derived suppressor cells are Associated with Progression of Human NSCLC

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A Novel Subset of B7-H3+CD14+HLA-DR-/low Myeloid-derived sup...

第十四届全国肿瘤生物治疗大会

作者： guangbo zhang Haitao Huang Jianquan Hou Xueguang zhang Clinical Immunology Laboratory The First Affiliated Hospital of Soochow University Institute of Medical Biotechnology The First Affiliated Hospital of Soochow University Department of Thoracic Surgery The First Affiliated Hospital of Soochow University Clinical Immunology Laboratory The First Affiliated Hospital of Soochow University Institute of Medical Biotechnology The First Affiliated Hospital of Soochow University

Myeloid-derived suppressor cells(MDSC) potently inhibit antitumor immune responses, and thereby promoting tumor progression and metastasis. However, the nature of human tumor-infiltrating MDSC remains poorly characterized. Here, we found that B7-H3 was exclusively expressed on a subset of intratumoral CD14+HLA-DR-/low MDSC but not on those from adjacent normal lung tissues of the cancer patients. Cytokine analysis revealed that B7-H3+CD14+HLA-DR-/low MDSC(B7-H3+MDSC) produced higher levels of IL-10 and TNF-α but lower levels of the IL-1β and IL-6 when compared with B7-H3-CD14+HLA-DR-/low MDSC(B7-H3-MDSC). In a murine lung cancer model, B7-H3+MDSC were found only in the tumor microenvironment and their frequencies increased during tumor progression. Clinical data analysis indicated that higher frequencies of B7-H3+MDSC were associated with reduced recurrence-free survival in patients with NSCLC. Taken together, we identify a novel subset of MDSC within the tumor microenvironment that leads to tumor progression.

关键词： MDSC NSCLC B7-H3

面向倾斜实景模型的三维可视化平台建设技术研究

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测绘通报 2021年第S01期 277-281页

作者：张广波北京华星勘查新技术有限公司北京100035

为解决倾斜摄影测量生产的实景三维模型集中统一管理难度大、可视化程度低、数据共享困难等问题,本文研究了基于Cesium开源三维地球引擎库的数字高程模型和倾斜实景模型的数据融合处理技术方法,并构建了基于B/S架构的面向倾斜实景模型... 详细信息

为解决倾斜摄影测量生产的实景三维模型集中统一管理难度大、可视化程度低、数据共享困难等问题,本文研究了基于Cesium开源三维地球引擎库的数字高程模型和倾斜实景模型的数据融合处理技术方法,并构建了基于B/S架构的面向倾斜实景模型的三维可视化WebGIS平台,实现了倾斜实景模型与多源空间数据的叠加及动态展示、查询定位、空间量测等功能,可为基于3DGIS的实景三维城市、三维自然资源管理等系统开发提供技术参考。

关键词：倾斜实景模型三维可视化平台倾斜摄影测量 Cesium

TREM-1low is a novel marker for Tumor-associated macrophages in tumor microenvironment and associated with lung cancer progression

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TREM-1low is a novel marker for Tumor-associated macrophages...

第十届全国免疫学学术大会

作者： guangbo zhang Hongmei Liu Siwen Chen Haitao Huang Xueguang zhang Ling Wang Clinical Immunology Laboratory The First Affiliated Hospital of Soochow University Department of Special Procurement Ward The First Affiliated Hospital of Soochow University Department of Thoracic Surgery The First Affiliated Hospital of Soochow University

Objection:TREM-1 is a crucial molecule for triggering and magnifying inflammatory responses and plays an important role in sepsis,but its expression and function in tumor remains *** this study,we intent to explore the clinical significance and biofunctions of TREM-1 expressed on TAMs in lung ***:A total of 40 patients with NSCLC were enrolled in this *** and corresponding nontumor paired tissue samples were obtained by operation from NSCLC ***-1 expression on monocytes/macrophage was detected by flow cytometry,and analyzed its clinical *** a blocking antibody against Trem-1,we examined the biofunction of TREM-1 on TAM isolated from tumor microenviroment by analysis of cytokines *** a LLCtumor bearing mouse model,we further confirm the relevance between TREM-1 expression on TAMs and tumor ***,we explored the key transcription factor on regulating TREM-1 expression on F4/80+monocyes/macrophage using WT,T-bet KO and Eomes KO ***:The levels of TREM-1 on tissue-infiltrating monocytes/macrophage from tumor nest was significantly lower compared to those from corresponding nontumor tissues and peripheral blood *** analysis indicated that TREM-1 levels on TAMs significantly decrease with cancer stages *** tumor-bearing mouse model further confirmed that TREM-1 expression on TAM decrease with tumor *** vitro,we found blocking TREM-1 signalcould significantly enhance the secretion of IL-1β.Finally,we found T-bet but not eomes maybe is the key transcription factor of TREM-1 expression on monocytes/***:Trem-1 levels on TAM in tumor microenviroment were significantly lower than those in peripheral blood from patients with *** levels of TREM-1 decrease with tumor *** TREM-1 significantly enhance IL-1βsecretion,indicating that this molecule play an important role in antitumor effect mediated by monocytes/macrophage.

关键词： TREM-1 NSCLC monocyte/macrophage tumor microenvironment

B7H3 Enhances Aerobic Glycolysis in Colorectal Cancer Cells to Promote Chemoresistance

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B7H3 Enhances Aerobic Glycolysis in Colorectal Cancer Cells ...

第十三届全国免疫学学术大会

作者： Tongguo Shi Yanchao Ma Lei Cao guangbo zhang Weichang Chen Xueguang zhang The First Affiliated Hospital of Soochow University

Aerobic glycolysis confers on cancer cells a growth advantage providing energy and biosynthesis building *** protein B7-H3 is an immune checkpoint member of the B7 *** B7-H3 is abnormal expression in human colorectal cancer tissues, the exact role and molecular mechanism of B7-H3 in colorectal cancer remains ***, we determined the function of B7H3 in the colorectal cancer aerobic glycolysis and explored the potential molecular mechanism of B7H3 in the development of colorectal cancer *** of B7H3 increased the rate of glucose consumption and lactate production, whereas knockdown of B7H3 had the opposite ***, we found that B7H3 upregulated the key glycolytic proteins HK2 via activating STAT3 *** further demonstrated that enhanced aerobic glycolysis was essential for the role of B7H3 in ***, we found that a positive correlation between the aberrant expression of B7H3 and HK2 in clinical colorectal cancer *** together, our findings revealed a previously unappreciated function of B7H3 in colorectal cancer cell aerobic glycolysis and chemoresistance, providing potential multiple therapeutic targets through intervention of glycolysis for the treatment of CRC.

关键词： Colorectal cancer Aerobic glycolysis Chemoresistance B7H3 HK2

基于自组织映射网络的制造服务模块动态创建方法

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计算机集成制造系统 2017年第8期23卷 1765-1773页

作者：张卫冷建兴张广博唐任仲方水良浙江师范大学工学院智能制造工程技术中心浙江金华321004 浙江大学流体动力与机电系统国家重点实验室浙江杭州310027 剑桥大学工程系制造研究院英国剑桥CB3OFS 浙江大学海洋结构研究所海洋工程装备国家地方联合工程实验室浙江舟山316021

针对商业生态环境中制造服务运作的复杂性,建立了制造服务模块化理论模型。该模型包括制造服务模块的内涵和动态创建过程,初步实现了制造服务模块动态创建,为制造服务模块化奠定基础。应用突变理论构建了制造服务模块的产品度和服务度概... 详细信息

针对商业生态环境中制造服务运作的复杂性,建立了制造服务模块化理论模型。该模型包括制造服务模块的内涵和动态创建过程,初步实现了制造服务模块动态创建,为制造服务模块化奠定基础。应用突变理论构建了制造服务模块的产品度和服务度概念,并建立了制造服务模块测度模型及算法。在此基础上,提出制造服务模块动态聚类的自组织映射网络策略。通过制造服务模块动态聚类的示例,验证了制造服务模块测度模型的有效性,以及基于神经网络和模块化技术建模的优越性。

关键词：制造服务模块化突变理论自组织映射网络动态聚类策略

共刺激分子B7-H3对人非小细胞肺癌细胞系A549侵袭和存活影响的初步研究

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中华微生物学和免疫学杂志 2019年第5期39卷 327-332页

作者：张帅张东泽黄子逸张光波苏州大学附属第一医院临床免疫研究所江苏省临床免疫学重点实验室216007

目的探讨B7-H3分子对人非小细胞肺癌细胞系(A549)增殖和侵袭的影响。方法流式细胞术检测A549上B7-H3分子蛋白的表达,慢病毒感染A549以干扰B7-H3表达(B7-H3-A549),并通过Westernblot和qPCR对其进行鉴定;CCK8法测定B7-H3-A549和B7-H3^+A549两组细胞的增殖差异;Annexin-PE/PI双染和PI单染后,流式检测细胞凋亡和周期的变化;Transwell法评估B7-H3^-A549和B7-H3^+A549细胞迁移和侵袭的变化;Westernblot检测细胞凋亡相关蛋白的表达。结果A549高表达B7-H3蛋白分子,感染慢病毒后得到B7-H3-A549稳定表达的细胞株及其对照细胞株B7^-H3^+A549。A549沉默B7-H3后生长速度显著降低。B7-H3-A549组早期凋亡细胞百分率高于B7-H3^+A549,两组晚期细胞凋亡率差异无统计学意义;B7-H3^-A549细胞在细胞周期G0/G1期受到阻滞。B7-H3^-A549细胞的迁移和侵袭能力低于B7-H3+A549细胞。B7-H3-A549细胞中Bad、Caspase-3表达上调,Bcl-2、P-AKT、MMP-9表达下调,总AKT没有明显变化。结论沉默B7-H3分子抑制了A549的增殖和侵袭,促使细胞阻滞于G0/G1期,诱导细胞发生凋亡。

关键词： A549细胞 B7-H3 侵袭凋亡

CAR-T细胞免疫疗法临床研究伦理问题探析

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免疫学杂志 2020年第11期36卷 921-925页

作者：周丽张光波苏州大学附属第一医院科技处 215000 苏州大学附属第一医院临床免疫研究所/江苏省临床免疫研究所 215000

探讨与分析CAR-T细胞免疫疗法临床研究的现状与存在的伦理问题,结合本中心伦理审查的实践,为推进CART细胞产品及细胞治疗临床研究在国内健康开展、规范我国细胞免疫治疗研究的伦理标准、保障受试者安全与权益提供参考。方法国内CAR-T细... 详细信息

探讨与分析CAR-T细胞免疫疗法临床研究的现状与存在的伦理问题,结合本中心伦理审查的实践,为推进CART细胞产品及细胞治疗临床研究在国内健康开展、规范我国细胞免疫治疗研究的伦理标准、保障受试者安全与权益提供参考。方法国内CAR-T细胞免疫疗法临床研究发展迅猛,但存在很多未知风险,结合CAR-T细胞免疫疗法国内外研究概况、细胞免疫疗法临床研究存在的伦理问题、本中心伦理委员会在CAR-T细胞免疫临床研究审查中的实践经验进行分析与论述。CAR-T细胞免疫疗法仍属于新兴技术,在目前开展的临床研究中,其技术标准、临床疗效与潜在风险尚需长期更多数据的积累,伦理审查中应了解新技术的发展,结合相关法规,完善伦理审查体系,把握伦理审查的要点,持续跟踪审查,从多角度确保受试者的安全与权益。在CAR-T细胞免疫疗法临床研究的发展中,加强伦理建设是科学研究的重要组成部分。伦理委员会应不断完善审查体系,提升认知和审查能力,科学监管、持续跟踪才能推进CAR-T产品及细胞治疗技术在国内健康发展,让这项新技术惠及更多的肿瘤患者。

关键词： CAR-T 细胞免疫疗法临床研究伦理审查

A Novel Subset of B7-H3+CD14+HLA-DR-/low Myeloid-derived suppressor cells are Associated with Progression of Human NSCLC

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A Novel Subset of B7-H3+CD14+HLA-DR-/low Myeloid-derived sup...

第九届全国免疫学学术大会

作者： guangbo zhang Haitao Huang Jianquan Hou Yibei Zhu Gehua Yu Xin Gao Yunyun Xu Xueguang zhang Clinical Immunology Laboratory The First Affiliated Hospital of Soochow University Institute of Medical Biotechnology The First Affiliated Hospital of Soochow University Department of Thoracic Surgery The First Affiliated Hospital of Soochow University

Myeloid-derived suppressor cells(MDSC)potently inhibit antitumor immune responses,and thereby promoting tumor progression and ***,the nature of human tumor-infiltrating MDSC remains poorly ***,we found that B7-H3 was exclusively expressed on a subset of intratumoral CD14HLA-DRMDSC but not on those from adjacent normal lung tissues of the cancer *** analysis revealed that B7-H3CD14HLADRMDSC(B7-H3MDSC)produced higher levels of IL-10 and TNF-a but lower levels of the IL-1βand IL-6when compared with B7-H3CD14HLA-DRMDSC(B7-H3MDSC).In a murine lung cancer model,B7-H3MDSC were found only in the tumor microenvironment and their frequencies increased during tumor *** data analysis indicated that higher frequencies of B7-H3MDSC were associated with reduced recurrence-free survival in patients with *** together,we identify a novel subset of MDSC within the tumor microenvironment that leads to tumor progression.

关键词： MDSC NSCLC B7-H3