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An efficient method for the sanitary vitrification of bovine oocytes in straws

Journal of Animal Science and Biotechnology 2014年第4期5卷 399-405页

作者： Yanhua zhou Xiangwei Fu guangbin zhou Baoyu Jia Yi Fang Yunpeng Hou Shien Zhu National Engineering Laboratory for Animal Breeding Key Laboratory of Animal geneticsBreeding and ReproductionMinistry of AgricultureCollege of Animal Science and TechnologyChina Agricultural University Institute of Animal Genetics and Breeding College of Animal Science and TechnologySichuan Agricultural University(Chengdu Campus) State Key Laboratory for Agrobiotechnology College of Biological SciencesChina Agricultural University

Background：At present,vitrification has been widely applied to humans,mice and farm *** improve the efficiency of vitrification in straw,bovine oocytes were used to test a new two-step vitrification method in this ***：When in vitro matured oocytes were exposed to 20%ethylene glycol（EG20） for 5 min and 40%ethylene glycol（EG40） for 30 s,followed by treatment with 30%glycerol（Gly30）,Gly40 or Gly50,a volume expansion was observed in Gly30 and Gly40 but not *** indicates that the intracellular osmotic pressure after a 30 s differs between EG40 and ranged between Gly40（approximately 5.6 mol/L） and Gly50（approximately 7.0 mol/L）.Since oocytes are in EG40 just for only a short period of time（30 s） and at a lower temperature（4℃）,we hypothesize that the main function of this step in to induce *** on these results,we omitted the EG40 step,before oocytes were pretreated in EG20 for 5 min,exposed to pre-cooled（4℃） Gly50,for 30 s,and then dipped into liquid *** warming,81.1%of the oocytes survived,and the surviving oocytes developed into cleavage stage embryos（63.5%） or blastocysts（20.0%） after parthenogenetic ***：These results demonstrate that in a two-step vitrification procedure,the permeability effect in the second step is not *** is possible that the second step is only required to provide adequate osmotic pressure to condense the intracellular concentration of CPAs to a level required for successful vitrification.

关键词： Bovine Cryopreservation Oocytes Straw Vitrification

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No effect of exogenous melatonin on development of cryopreserved metaphase Ⅱ oocytes in mouse

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Journal of Animal Science and Biotechnology 2015年第4期6卷 388-394页

作者： Wei Li Keren Cheng Yue Zhang Qinggang Meng Shi'en Zhu guangbin zhou Institute of Animal Genetics and Breeding College of Animal Science and TechnologySichuan Agricultural University(Chengdu Campus) Nanjing Biomedical Research Institute of Nanjing University College of Animal Science and Technology China Agricultural University Department of Animal Dairyand Veterinary SciencesUtah State University

Background： This study was conducted to investigate effect of exogenous melatonin on the development of mouse mature oocytes after cryopreservation. Results： First, mouse metaphase II （MII） oocytes were vitrified in the open-pulled straws （OPS）. After warming, they were cultured for 1 h in M2 medium containing melatonin at different concentrations （0, 10-9, 10-7, 10-s, 10 3 mol/L）. Then the oocytes were used to detect reactive oxygen species （ROS） and glutathione （GSH） levels （fluorescence microscopy）, and the developmental potential after parthenogenetic activation. The experimental results showed that the ROS level and cleavage rate in 10-3 mol/L melatonin group was significantly lower than that in melatonin-free group （control）. The GSH levels and blastocyst rates in all melatonin-treated groups were similar to that in control. Based on the above results, we detected the expression of gene Hsp9Oaal, Hsfl, Hspalb, Nrf2 and Bcl-xl with qRT-PCR in oocytes treated with 10-7, or 10-3 mol/L melatonin and untreated control. After warming and culture for 1 h, the oocytes showed higher Hsp90oal expression in 10-7 mol/L melatonin-treated group than in the control （P 〈 0.05）; the Hsfl, Hsp9Oaal and Bcl-xl expression were significantly decreased in 10-3 mol/L melatonin-treated group when compared to the control. Based on the above results and previous research, we detected the development of vitrified-warmed oocytes treated with either 10-7 or 0 mol/L melatonin by in vitro fertilization. No difference was observed between them. Conclusions： Our results indicate that the supplementation of melatonin （10-9 to 10-3 mol/L） in culture medium and incubation for 1 h did not improve the subsequent developmental potential of vitrified-warmed mouse MII oocytes, even if there were alteration in gene expression.

关键词： Gene expression Melationin Mouse oocyte Parthenogenetic activation vitrificantion

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Melatonin improves the first cleavage of parthenogenetic embryos from vitrified-warmed mouse oocytes potentially by promoting cell cycle progression

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Journal of Animal Science and Biotechnology 2022年第1期13卷 106-122页

作者： Bo Pan Izhar Hyder Qazi Shichao Guo Jingyu Yang Jianpeng Qin Tianyi Lv Shengqin Zang Yan Zhang Changjun Zeng Qingyong Meng Hongbing Han guangbin zhou Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province College of Animal Science and TechnologySichuan Agricultural UniversityChengdu 611130China Department of Veterinary Anatomy and Histology Shaheed Benazir Bhutto University of Veterinary and Animal SciencesSakrandSindh 67210Pakistan State Key Laboratory of AgroBiotechnology China Agricultural UniversityBeijing 100193China National Engineering Laboratory for Animal Breeding Key Laboratory of Animal Genetics and Breeding of the Ministry of AgricultureBeijing Key Laboratory for Animal Genetic ImprovementCollege of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China

Background:This study investigated the effect of melatonin(MT)on cell cycle(G1/S/G2/M)of parthenogenetic zygotes developed from vitrified-warmed mouse metaphase II(MII)oocytes and elucidated the potential mechanism of MT action in the first cleavage of ***:After vitrification and warming,oocytes were parthenogenetically activated(PA)and in vitro cultured(IVC).Then the spindle morphology and chromosome segregation in oocytes,the maternal mRNA levels of genes including Miss,Doc1r,Setd2 and Ythdf2 in activated oocytes,pronuclear formation,the S phase duration in zygotes,mitochondrial function at G1 phase,reactive oxygen species(ROS)level at S phase,DNA damage at G2 phase,early apoptosis in 2-cell embryos,cleavage and blastocyst formation rates were *** results indicated that the vitrification/warming procedures led to following perturbations 1)spindle abnormalities and chromosome misalignment,alteration of maternal mRNAs and delay in pronucleus formation,2)decreased mitochondrial membrane potential(MMP)and lower adenosine triphosphate(ATP)levels,increased ROS production and DNA damage,G1/S and S/G2 phase transition delay,and delayed first cleavage,and 3)increased early apoptosis and lower levels of cleavage and blastocyst *** results further revealed that such negative impacts of oocyte cryopreservation could be alleviated by supplementation of warming,recovery,PA and IVC media with 10^(−9) mol/L MT before the embryos moved into the 2-cell stage of ***:MT might promote cell cycle progression via regulation of MMP,ATP,ROS and maternal mRNA levels,potentially increasing the first cleavage of parthenogenetic zygotes developed from vitrified-warmed mouse oocytes and their subsequent development.

关键词： Cell cycle Cleavage rate Melatonin MetaphaseⅡoocyte Parthenogenetic activation Vitrification

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Magneto‑Dielectric Synergy and Multiscale Hierarchical Structure Design Enable Flexible Multipurpose Microwave Absorption and Infrared Stealth Compatibility

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Nano-Micro Letters 2025年第2期17卷 401-416页

作者： Chen Li Leilei Liang Baoshan Zhang Yi Yang guangbin Ji School of Electronic Science and Engineering Nanjing UniversityNanjing 210093People’s Republic of China College of Materials Science and Technology Nanjing University of Aeronautics and AstronauticsNanjing 210016People’s Republic of China

Developing advanced stealth devices to cope with radar-infrared(IR)fusion detection and diverse application scenarios is increasingly demanded,which faces significant challenges due to conflicting microwave and IR cloaking mechanisms and functional integration ***,we propose a multiscale hierarchical structure design,integrating wrinkled MXene IR shielding layer and flexible Fe_(3)O_(4)@C/PDMS microwave absorption *** top wrinkled MXene layer induces the intensive diffuse reflection effect,shielding IR radiation signals while allowing microwave to pass ***,the permeable microwaves are assimilated into the bottom Fe_(3)O_(4)@C/PDMS layer via strong magneto-electric *** theoretical and experimental optimization,the assembled stealth devices realize a near-perfect stealth capability in both X-band(8–12 GHz)and long-wave infrared(8–14μm)wavelength ***,it delivers a radar cross-section reduction of−20 dB m^(2),a large apparent temperature modulation range(ΔT=70℃),and a low average IR emissivity of ***,the optimal device demonstrates exceptional curved surface conformability,self-cleaning capability(contact angle≈129°),and abrasion resistance(recovery time≈5 s).This design strategy promotes the development of multispectral stealth technology and reinforces its applicability and durability in complex and hostile environments.

关键词： Microwave absorption Radar-infrared compatible stealth Wrinkled MXene Magneto-dielectric synergy Multifunction

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GSM短信息智能控制器的设计与实现

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微计算机信息 2007年第1S期23卷 24-25,37页

作者：周光彬曾孝平重庆大学

本文提出了一种基于GSM平台的短信息智能控制系统。该系统以GSM网络为平台,利用短信息实现对远端设备的控制。给出了系统硬件结构和软件框图,试验结果表明,该系统可实现长距离控制,稳定可靠,费用低廉,可广泛应用于电器设备的远程控制。

关键词：短信息遥控单片机 DTMF

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全球电信业平稳发展

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信息网络 2005年第3期 5-9,13页

作者：周光斌信息产业部电信研究院通信政策研究所

全球电信业开始回暖从20世纪90年代后期由3G牌照引发的电信泡沫,到本世纪初的泡沫破灭.加上美国WorldCom和其他一些公司的财务丑闻接二连三曝光,公司纷纷破产,电信业的信誉受到空前的蒙羞和挑战,使本来陷入低迷的全球电信业跌入低谷.业... 详细信息

全球电信业开始回暖从20世纪90年代后期由3G牌照引发的电信泡沫,到本世纪初的泡沫破灭.加上美国WorldCom和其他一些公司的财务丑闻接二连三曝光,公司纷纷破产,电信业的信誉受到空前的蒙羞和挑战,使本来陷入低迷的全球电信业跌入低谷.业界受到的打击和经济损失不亚于1997年全球金融危机.

关键词：电信业电信运营商宽带业务市场结构电信业务

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全球IPTV市场与监管

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世界电信 2006年第2期19卷 33-38页

作者：周光斌信息产业部电信研究院通信政策研究所

追溯了全球各主要电信运营商推出IPTV业务的时间,展望了2008年IPTV市场的发展规模。阐述了北美市场、欧洲市场以及亚太市场的IPTV发展情况。分析了目前全球管制机构普遍遇到的问题,比如如何界定IPTV业务性质、由谁监管以及与IPTV有关的... 详细信息

追溯了全球各主要电信运营商推出IPTV业务的时间,展望了2008年IPTV市场的发展规模。阐述了北美市场、欧洲市场以及亚太市场的IPTV发展情况。分析了目前全球管制机构普遍遇到的问题,比如如何界定IPTV业务性质、由谁监管以及与IPTV有关的版权和其它问题。

关键词： IPTV 市场监管版权

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Molybdenum and Cadmium Co-induce Mitochondrial Quality Control Disorder via FUNDC1-Mediated Mitophagy in Sheep Kidney

Molybdenum and Cadmium Co-induce Mitochondrial Quality Contr...

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中国毒理学会第十次全国毒理学大会

作者： Yunhui Wu Fan Yang guangbin zhou Qi Wang Chenghong Xing He Bai Xin Yi Zhiwei Xiong Shuqiu Yang Huabin Cao Jiangxi Provincial Key Laboratory for Animal Health Institute of Animal Population HealthCollege of Animal Science and Technology Jiangxi Agricultural University

Molybdenum(Mo), fundamental trace mineral for animals and plants, but undue Mo damages animal health. Cadmium(Cd) is a toxic heavy metal that exists in the environment. Nevertheless, the mechanism of Mo and Cd on mitochondrial quality control are still indistinct. The objective of this research was to explore the effects of mitophagy on mitochondrial quality control via the FUNDC1-mediated by Mo and Cd in sheep kidney. Forty-eight 2-month-old sheep were stochastically divided into four groups, as shown below: control group, Mo [45 mg/kg body weight(BW)] group, Cd(1 mg/kg BW) group and Mo(45 mg/kg BW)+Cd(1 mg/kg BW) group, with 50 days feed technique. The results showed that Mo or/and Cd attract an unbalance of trace minerals and vacuoles and granular degeneration of renal tubular epithelial cells, and increase the number of mitophagosomes and vacuole-mitochondria and LC3 puncta and MDA and H2O2contents, and decrease ATP content in the kidney. Moreover, Mo or/and Cd treatment could upregulate the mRNA levels of FUNDC1, LC3A, LC3B, PGAM5,DRP1, FIS1 and MFF, and the protein levels of FUNDC1, p-FUNDC1, LC3Ⅱ/LC3Ⅰ, DRP1, MFF and FIS1, downregulate the mRNA levels of MFN1, MFN2, OPA1, PGC-1, SIRT1, SIRT3, FOXO1 and FOXO3, and the protein levels of MFN1, MFN2, OPA1 and PGC-1α. Notably, variations of above-mentioned factors in Mo and Cd group were more obvious than in Mo or Cd groups. T aken together, these results displayed that Mo and Cd co-treatment might induce mitochondrial quality control disorder via FUNDC1-mediated mitophagy in sheep kidney.

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法国发放3G许可证的做法

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世界电信 2001年第3期14卷 32-35页

作者：周光斌信息产业部电信科技情报研究所

３Ｇ牌照争夺近来成为全球瞩目的焦点。有的国家为了防止企业不正当的竞争和个别企业垄断ＵＭＴＳ市场，政府通过拍卖许可证的办法来规范市场竞争，为企业划定统一起跑线。如英国拍出３５０亿美元。德国拍出４５０亿美元，一时间各媒体... 详细信息

３Ｇ牌照争夺近来成为全球瞩目的焦点。有的国家为了防止企业不正当的竞争和个别企业垄断ＵＭＴＳ市场，政府通过拍卖许可证的办法来规范市场竞争，为企业划定统一起跑线。如英国拍出３５０亿美元。德国拍出４５０亿美元，一时间各媒体上亦拍声鹊起。然而，事实上各国的许可证发放各有打算，如瑞典强调消遇者的权利，坚持走“评审制”；法国政府也宣布，将采用评选方式发放３Ｇ许可证。

关键词： 3G牌照许可证发放移动通信电信业

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2006通信企业“走出去”迈出新步伐

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世界电信 2006年第12期19卷 7-11页

作者：周光斌信息产业部电信研究院通信政策与管理研究所

“走出去”已经成为我国通信设备制造商和电信运营商的共同选择,2006年,在这一战略的实施上,我国通信设备制造商和电信运营商更是迈出了新的步伐,文章围绕以下六个方面进行了深入阐述:政府强力推动,企业调整方式,发挥港澳地区作用,积极... 详细信息

“走出去”已经成为我国通信设备制造商和电信运营商的共同选择,2006年,在这一战略的实施上,我国通信设备制造商和电信运营商更是迈出了新的步伐,文章围绕以下六个方面进行了深入阐述:政府强力推动,企业调整方式,发挥港澳地区作用,积极探讨非洲,制造企业主打,运营企业跟进。并以这几个方面为主,结合实例进行了具体说明,并提出了相关的建议,希望借此为运营企业和通信设备制造商在下一步的“走出去”战略中提供有益的借鉴。

关键词：走出去推动调整主打跟进

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