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Knockout of miR396 genes increases seed size and yield in soybean

Journal of Integrative Plant Biology 2024年第6期66卷 1148-1157页

作者： Hongtao Xie Fei Su Qingfeng Niu Leping Geng Xuesong Cao Minglei Song Jinsong dong Zai Zheng rui Guo Yang Zhang Yuanwei Deng Zhanbo Ji Kang Pang Jian‐Kang Zhu Jianhua Zhu School of Life Sciences Anhui Agricultural UniversityHefei 230036China Research Center for Biological Breeding Technology Research Institute of Frontier ScienceAnhui Agricultural UniversityHefei 230036China Bellagen Biotechnology Co.Ltd Jinan 250000China Institute of Crop Sciences/National Nanfan Research Institute Chinese Academy of Agricultural SciencesKey Laboratory of Gene Editing Technologies(Hainan)Ministry of Agriculture and Rural AffairsSanya 572025China Southern University of Science and TechnologyShenzhen 518055China Hainan Yazhou Bay Seed Laboratory Sanya 572024China

Yield improvement has long been an important task for soybean breeding in the world in order to meet the increasing demand for food and animal *** R396 genes have been shown to negatively regulate grain size in rice,but whether mi R396 family members may function in a similar manner in soybean is ***,we generated eight soybean mutants harboring different combinations of homozygous mutations in the six soybean mi R396genes through genome editing with clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated nuclease(Cas)12SF01 in the elite soybean cultivar Zhonghuang 302(ZH302).Four triple mutants(mir396aci,mir396acd,mir396adf,and mir396cdf),two quadruple mutants(mir396-abcd and mir396acfi),and two quintuple mutants(mir396abcdf and mir396bcdfi)were *** found that plants of all the mir396 mutants produced larger seeds compared to ZH302 *** tests showed that mir396adf and mir396cdf plants have significantly increased yield in growth zones with relatively high latitude which are suited for ZH302 and moderately increased yield in lower *** contrast,mir396abcdf and mir396bcdfiplants have increased plant height and decreased yield in growth zones with relatively high latitude due to lodging issues,but they are suited for low latitude growth zones with increased yield without lodging *** together,our study demonstrated that loss-of-function of mi R396 genes leads to significantly enlarged seed size and increased yield in soybean,providing valuable germplasms for breeding high-yield soybean.

关键词： Cas12SF01 CRISPR/Cas miR396 seed size soybean yield

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A Review on Particle Reinforced Mg Matrix Composites Fabricated by Powder Metallurgy

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Acta Metallurgica Sinica(English Letters) 2024年第3期37卷 391-400页

作者： Zhiyuan Liu Li Jin Jian Zeng Fulin Wang Fenghua Wang Shuai dong Jie dong National Engineering Research Center of Light Alloy Net Forming and State Key Laboratory of Metal Matrix Composite School of Materials Science and EngineeringShanghai Jiao Tong UniversityShanghai 200240China

This paper provides a comprehensive review of research progress in particle-reinforced Mg matrix composites prepared via powder *** article discusses different strategies,such as micro-sized,nano-sized particles,and multi-particle hybridization,which has been employed to enhance the performance of the *** addition,a range of preparation techniques that optimize the dispersion of the reinforcing particles are *** paper also highlights how the different configurations between the reinforcements and matrix alloy impact the composites’***,the article outlines the prospects of particles reinforced Mg matrix composites fabricated via powder metallurgy and recommends modification methods that could be explored to further develop these materials for various applications.

关键词： Magnesium matrix composites Reinforcing particle Powder metallurgy Mechanical properties Configuration

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Carbonic anhydrase-like enzymes in the formation of Lycopodium alkaloid

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Chinese Journal of Natural Medicines 2024年第3期22卷 193-194页

作者： dong Huiwen GE Huiming State key Laboratory of Pharmaceutical Biotechnology Institute of Functional BiomoleculesSchool of Life SciencesNanjing UniversityNanjing 210023China

Plant alkaloids,renowned for their structural diversity and bioactivity,are prominent in both modern and traditional medicine[1].Unraveling the intricacies of plant alkaloid biosynthesis could pave the way for the discovery of new natural products and *** may also shed light on the roles of existing pathways in host biology and provide valuable tools for metabolic engineering in plants and microbes[2].Unlike other major classes of plant natural products,such as terpenoids and polyketides,the formation of alkaloid scaffolds does not conform to a uniform chemical theme or depend on a singular enzyme class[3].A case in point is the Lycopodium alkaloids in the Lycopodiaceae family,where the enzymes responsible for their core scaffold construction remain unidentified[4].

关键词： Lycopodium alkaloid Carbonic anhydrase-like enzymes Biosynthesis

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Pure State Feedback Switching Control Based on the Online Estimated State for Stochastic Open Quantum Systems

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IEEE/CAA Journal of Automatica Sinica 2024年第10期11卷 2166-2178页

作者： Shuang Cong Zhixiang dong Department of Automation University of Science and Technology of ChinaHefei 230027China Lazas Network Technology Co.Ltd Hangzhou 311100China

For the n-qubit stochastic open quantum systems,based on the Lyapunov stability theorem and LaSalle’s invariant set principle,a pure state switching control based on on-line estimated state feedback(short for OQST-SFC)is proposed to realize the state transition the pure state of the target state including eigenstate and superposition *** proposed switching control consists of a constant control and a control law designed based on the Lyapunov method,in which the Lyapunov function is the state distance of the *** constant control is used to drive the system state from an initial state to the convergence domain only containing the target state,and a Lyapunov-based control is used to make the state enter the convergence domain and then continue to converge to the target *** the same time,the continuous weak measurement of quantum system and the quantum state tomography method based on the on-line alternating direction multiplier(QST-OADM)are used to obtain the system information and estimate the quantum state which is used as the input of the quantum system ***,the pure state feedback switching control method based on the on-line estimated state feedback is realized in an n-qubit stochastic open quantum *** complete derivation process of n-qubit QST-OADM algorithm is given;Through strict theoretical proof and analysis,the convergence conditions to ensure any initial state of the quantum system to converge the target pure state are *** proposed control method is applied to a 2-qubit stochastic open quantum system for numerical simulation *** possible different position cases between the initial estimated state and that of the controlled system are studied and discussed,and the performances of the state transition under the corresponding cases are analyzed.

关键词： Online state estimation state feedback control stochastic open quantum systems(OQST) switching control

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On asymptotic stability of the 3D Boussinesq equations without heat conduction

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Science China Mathematics 2024年第2期67卷 253-280页

作者： Lihua dong Yongzhong Sun School of Physical and Mathematical Sciences Nanjing Tech UniversityNanjing 211816China Department of Mathematics Nanjing UniversityNanjing 210093China

We investigate the asymptotic stability of the solutions to Boussinesq equations without heat conduction with the initial data near a specific stationary solution in the three-dimensional domain Ω=R^(2)×(0,1).It is shown that the solution starting from a small perturbation to the stationary solution converges to it with explicit algebraic rates as time tends to infinity.

关键词： Boussinesq equations stationary solutions asymptotic stability decay rates

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Real-time in-situ monitoring of chiral changes during a reaction at single-molecule level

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Science China Chemistry 2024年第2期67卷 422-423页

作者： Min Tan dong Xiang Institute of Modern Optics and Center of Single-Molecule Science Tianjin Key Laboratory of Micro-scale Optical Information Science and TechnologyNankai UniversityTianjin300350China School of Materials Science and Engineering Smart Sensing Interdisciplinary Science CenterNankai UniversityTianjin300350China TongLu FeiRan Garment–Nankai University Joint Laboratory Nankai UniversityTianjin300350China

Molecular chirality is a widespread phenomenon in nature,playing a critical role in various fields such as chemistry [1],life sciences [2], and materials science [3], and has therefore attracted significant attention and research by scholars [4–6].However, the chirality in chemical reactions is often dynamic, making real-time monitoring of chiral changes during chemical reactions at the single-molecule level extremely challenging.

关键词： chiral chirality critical

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Research on Alliance Decision of Dual-Channel Remanufacturing Supply Chain Considering Bidirectional Free-Riding and Cost-Sharing

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Computer Modeling in Engineering & Sciences 2024年第9期140卷 2913-2956页

作者： Lina dong Yeming Dai School of Business Qingdao UniversityQingdaoChina

This study delves into the formation dynamics of alliances within a closed-loop supply chain(CLSC)that encom-passes a manufacturer,a retailer,and an e-commerce *** leverages Stackelberg game for this exploration,contrasting the equilibrium outcomes of a non-alliance model with those of three differentiated alliance *** non-alliance model acts as a crucial benchmark,enabling the evaluation of the motivations for various supply chain entities to engage in alliance *** analysis is centered on identifying the most effective alliance strategies and establishing a coordination within these *** thoroughly investigate the consequences of diverse alliance behaviors,bidirectional free-riding and cost-sharing,and the resultant effects on the optimal decision-making among supply chain *** findings underscore several pivotal insights:(1)The behavior of alliances within the supply chain exerts variable impacts on the optimal pricing and demand of its *** comparison to the non-alliance(D)model,the manufacturer-retailer(MR)and manufacturer-e-commerce platform(ME)alliances significantly lower both offline and online resale prices for new and remanufactured *** adjustment leads to an enhanced demand for products via the MR alliance’s offline outlets and the ME alliance’s online platforms,thereby augmenting the profits for those within the ***,retailer-e-commerce platform(ER)alliance tends to increase the optimal retail price and demand across both online and offline *** specific conditions,alliance behavior can also increase the profits of non-alliance members,and the profits derived through alliance channels also exceed those from non-alliance channels.(2)The prevalence of bidirectional free-riding behavior largely remains constant across different alliance *** these models,bidirectional free-riding typically elevates the equilibrium prices in offline channel while negatively affe

关键词： Dual-channel remanufacturing supply chain alliance remanufactured products bidirectional free-riding cost-sharing

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Fusion of Spiral Convolution-LSTM for Intrusion Detection Modeling

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Computers, Materials & Continua 2024年第5期79卷 2315-2329页

作者： Fei Wang Zhen dong School of Cyberspace Security Gansu University of Political Science and LawLanzhou73000China

Aiming at the problems of low accuracy and slow convergence speed of current intrusion detection models,SpiralConvolution is combined with Long Short-Term Memory Network to construct a new intrusion detection *** dataset is first preprocessed using solo thermal encoding and normalization *** the spiral convolution-Long Short-Term Memory Network model is constructed,which consists of spiral convolution,a two-layer long short-term memory network,and a *** is shown through experiments that the model is characterized by high accuracy,small model computation,and fast convergence speed relative to previous deep learning *** model uses a new neural network to achieve fast and accurate network traffic intrusion *** model in this paper achieves 0.9706 and 0.8432 accuracy rates on the NSL-KDD dataset and the UNSWNB-15 dataset under five classifications and ten classes,respectively.

关键词： Intrusion detection deep learning spiral convolution long and short term memory networks 1D-spiral convolution

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Could π-aromaticity cross an unsaturated system to a fully saturated one?

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Chinese Chemical Letters 2024年第6期35卷 298-302页

作者： Shicheng dong Jun Zhu State Key Laboratory of Physical Chemistry of Solid Surfaces Collaborative Innovation Center of Chemistry for Energy Materials(iChem)Fujian Provincial Key Laboratory of Theoretical and Computational ChemistryCollege of Chemistry and Chemical EngineeringXiamen UniversityXiamen 361005China School of Science and Engineering The Chinese University of Hong KongShenzhen 518172China

The classification of π-/σ-aromaticity depends on the electrons with the dominating ***,π-andσ-aromaticity are used to describe the unsaturated and saturated systems,***,it is rarely reported that π-aromaticity is dominated in a saturated *** we demonstrate that π-aromaticity could be dominating in several fully saturated four-membered rings(4MRs),supported by various aromaticity indices including ΔBL,NICS,EDDB,MCI,and Ad *** origin of suchπ-aromaticity in saturated rings could be attributed to an introduction of two additional electrons into the π-type LUMO of the parent neutral *** findings represent a novel approach to achieve π-aromaticity into a fully saturated system which has traditionally been dominated by σ-aromaticity.

关键词： π-Aromaticity Saturated rings Silicon

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Observation of gapless corner modes of photonic crystal slabs in synthetic translation dimensions

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Photonics Research 2024年第3期12卷 444-455页

作者： WEN-JIN ZHANG HAO-CHANG MO WEN-JIE CHEN XIAO-dong CHEN JIAN-WEN dong School of Physics&State Key Laboratory of Optoelectronic Materials and Technologies Sun Yat-sen UniversityGuangzhou 510275China

Second-order topological photonic crystals support localized corner modes that deviate from the conventional bulk-edge ***,the frequency shift of corner modes spanning the photonic band gap has not been experimentally ***,we observe the gapless corner modes of photonic crystal slabs within a parameter space by considering translation as an additional synthetic *** corner modes,protected by topological pumping in synthetic translation dimensions,are found to exist independently of the specific corner *** gapless corner modes are experimentally imaged via the near-field scanning measurement and validated numerically by full-wave *** propose a topological rainbow with gradient translation,demonstrating the ability to extract and separate specific frequency components of light into different spatial *** work contributes to the advancement of topological photonics and provides valuable insights into the exploration of gapless corner modes in synthetic dimensions.

关键词： translation dimensions corner

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