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World Journal of Gastroenterology 2008年第36期14卷 5564-5569页

作者： Jian-Qing Yang guang-Dong Pan guang-Ping chu Zhen Liu Qiang Liu Yi Xiao Lin Yuan Department of Surgery The People's Hospital of Liuzhou City Liuzhou 545001Guangxi Zhuang Autonomous Region China

AIM: To investigate the effects of interferon-alpha (IFN-α) to restrain the growth and invasive potential of hepatocellular carcinoma (HCC) induced by hepatitis B virus (HBV) X protein. METHODS: The pcDNA3.1-HBx plasmid was transfected into Chang cells by Lipofectamine in vitro, and Chang/HBx was co-cultured with IFN-α. Cell survival growth curve and clonogenicity assay were used to test the growth potential of Chang/pcDNA3.1, Chang/HBx and IFN-α-Chang/HBx in vitro. Growth assay in nude mice was used to detect the growth potential of Chang/ pcDNA3.1, Chang/HBx and IFN-α-Chang/HBx in vivo. Wound healing and transwell migration assays were used to detect the invasive ability of Chang/pcDNA3.1, Chang/HBx and IFN-α-Chang/HBx. RESULTS: Compared with CCL13 cells transfected with pcDNA3.1, CCL13 with stable expression of hepatitis B virus X protein showed the characteristics of malignant cells with high capability of growth and invasion by detecting their growth curves, colony forming efficiency, wound healing , transwell migration assays and growth assays in nude mice. Its capability of growth and invasion could be controlled by IFN-α. CONCLUSION: IFN-α can restrain the growth and invasive potential of HCC cells induced by HBx protein, which has provided an experimental basis for IFN-α therapy of HCC.

关键词： Hepatitis B virus X protein Interferon-alpha Hepatocellular carcinoma Growth Invasion

Rhizosphere Aeration Improves Nitrogen Transformation in Soil, and Nitrogen Absorption and Accumulation in Rice Plants

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Rice science 2020年第2期27卷 162-174页

作者： XU chunmei CHEN Liping CHEN Song chu guang WANG Danying ZHANG Xiufu China National Rice Research Institute Chinese Academy of Agricultural SciencesHangzhou 310006China

Two rice cultivars(Xiushui 09 and chunyou 84)were used to evaluate the effects of various soil oxygen(O2)conditions on soil nitrogen(N)transformation,absorption and accumulation in rice *** treatments were continuous flooding(CF),continuous flooding and aeration(CFA),and alternate wetting and drying(AWD).The results showed that the AWD and CFA treatments improved soil N transformation,rice growth,and N absorption and *** NO3–content,nitrification activity and ammonia-oxidising bacteria abundance,leaf area,nitrate reductase activity,and N absorption and accumulation in rice all increased in both ***,soil microbial biomass carbon and pH did not significantly change during the whole period of rice *** analysis revealed a significant positive correlation between the nitrification activity and ammonia-oxidising bacteria abundance,and both of them significantly increased as the total N accumulation in rice *** results indicated that improved soil O2 conditions led to changing soil N cycling and contributed to increases in N absorption and accumulation by rice in paddy fields.

关键词： ammonium-nitrogen content nitrate-nitrogen content nitrification activity nitrate reductase activity rhizosphere oxygen condition ammonia-oxidising bacteria abundance rice nitrogen use efficiency

Complete kinetic model for esterification reaction of lauric acid with glycerol to synthesize glycerol monolaurate

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Chinese Journal of Chemical Engineering 2024年第6期70卷 211-221页

作者： Han-Qiao Hu Yue Zhang Ming Fan Yong Cai guang-Wen chu Liang-Liang Zhang Research Center of the Ministry of Education for High Gravity Engineering and Technology Beijing University of Chemical TechnologyBeijing 100029China State Key Laboratory of Organic-Inorganic Composites Beijing University of Chemical TechnologyBeijing 100029China Petrochemical Research Institute China National Petroleum CorporationBeijing 102206China

Glycerol monolaurate(GML)is a widely used industrial chemical with excellent emulsification and antibacterial *** direct esterification of glycerol with lauric acid is the main method to synthesize *** this work,the kinetic process of direct esterification was systematically studied using p-toluenesulfonic acid as catalyst.A complete kinetic model of consecutive esterification reaction has been established,and the kinetic equation of acid catalysis was *** isomerization reactions of GML and glycerol dilaurate were *** was found that the reaction was an equilibrium reaction and the reaction rate was faster than the esterification *** kinetic equations of the consecutive esterification reaction were obtained by experiments as k_(1)=(276+92261Xcat)exp(-37720/RT)and k_(2)=(80+4413Xcat)exp(-32240/RT).The kinetic results are beneficial to the optimization of operating conditions and reactor design in GML production process.

关键词： Glycerol monolaurate p-Toluenesulfonic acid Esterification Kinetics Mass transfer

Experimental investigation of the mixing efficiency via intensity of segregation along axial direction of a rotating bar reactor

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Chinese Journal of Chemical Engineering 2023年第7期59卷 153-159页

作者： Abdelgadir Bashir Banaga Yan-Bin Li Zhi-Hao Li Bao-Chang Sun guang-Wen chu State Key Laboratory of Organic-Inorganic Composite Beijing University of Chemical TechnologyBeijing 100029China Research Center of the Ministry of Education for High Gravity Engineering and Technology Beijing University of Chemical TechnologyBeijing 100029China Department of Chemical Engineering Tiangong UniversityTianjin 300387China

As a significant index to evaluate the mixing efficiency,studying the concentration distribution is directly related to the intensity of segregation(I_(s)).In this work,the I_(s) of the mixture composed of NaCl solutionwater was investigated experimentally in a rotating bar reactor(RBR)by the conductivity *** results showed that the mixing efficiency was improved along the axial direction from the bottom to the top in the *** concentration distribution at the bottom section was more uneven,and I_(s) was higher compared with the top section,which decreased from 6.53×10^(-5)to 1.57×10^(-7).With the increase of rotational speed from 0 to 700 r·min^(-1),I_s at the bottom and top sections decreased from 4.27×10^(-3)to 7.10×10^(-5)and from 1.93×10^(-3)to 7.29×10^(-7),*** increases flow rate of solution A,and the decreases of concentration of NaCl and flow rate of solution B gave rise to the reduction of I_(s),signifying an improved mixing *** results revealed that the conductivity method used in this paper has high efficiency and low cost to measure the I_(s),which indicates a promising prospect for estimating reactors'mixing performance.

关键词： Intensity of segregation Mixing efficiency Rotating bar reactor Conductivity method Concentration distribution

Agronomic performance of drought-resistance rice cultivars grown under alternate wetting and drying irrigation management in southeast China

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The Crop Journal 2018年第5期6卷 482-494页

作者： guang chu Tingting Chen Song Chen chunmei Xu Danying Wang Xiufu Zhang China National Rice Research Institute Chinese Academy of Agricultural Sciences

Compared to drought-susceptible rice cultivars(DSRs),drought-resistance rice cultivars(DRRs)could drastically reduce the amount of irrigation water input and simultaneously result in higher grain yield under water-saving irrigation ***,the mechanisms underlying these properties are *** investigated how improved agronomic traits contribute to higher yield and higher water use efficiency(WUE)in DRRs than in DSRs under alternate wetting and drying(AWD).Two DRRs and two DSRs were field-grown in 2015 and 2016 using two different irrigation regimes:continuous flooding(CF)and *** CF,no statistical differences in grain yield and WUE were observed between DRRs and *** water under the AWD regime was 275–349 mm,an amount 49.8%–56.2% of that(552–620 mm)applied under the CF *** to CF,AWD significantly decreased grain yield in both DRRs and DSRs,with a more significant reduction in DSRs,and WUE was increased in DRRs,but not in DSRs,by 9.9%–23.0% under *** AWD,DRRs showed a 20.2%–26.2% increase in grain yield and an 18.6%–24.5% increase in WUE compared to *** to DSRs,DRRs showed less redundant vegetative growth,greater sink capacity,higher grain filling efficiency,larger root biomass,and deeper root distribution under *** conclude that these improved agronomic traits exert positive influences on WUE in DRRs under AWD.

关键词： Agronomic traits Alternate wetting and drying Drought-resistance rice cultivars Grain yield Water use efficiency

Reversal of multi-drug resistance by pSUPER-shRNA-mdr1 in vivo and in vitro

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World Journal of Gastroenterology 2009年第4期15卷 431-440页

作者： guang-Dong Pan Jian-Qing Yang Lv-Nan Yan guang-Ping chu Qiang Liu Yi Xiao Lin Yuan Department of General Surgery People's Hospital of Liuzhou Medical Universityof Guangxi Liuzhou 545001 Guangxi Zhuang Autonomous Region China

AIM： To explore the possibility of reversing multi-drug resistance （MDR） to HepG2/mdr1 in vitro and in vivo with RNA interference （RNAi）. METHODS： HepG2/mdr1 was obtained by cloning the whole gene mdrl into HepG2 cells, shRNA targeting sequence was designed to be homologous to the P-gp encoding MDR1 mRNA consensus sequence, pSUPER- shRNA/mdrl was constructed using the enzyme- digested technique. HepG2/mdrl cells were transfected with vectors of pSUPER-shRNA/mdrl to measure their efficacy by real-time PCR for mdrl mRNA, flow cytometry （FCM） for P-gp expression, and Rhodamine efflux, MTT method for HepG2/mdrl function, respectively. In vivo, mice tumors were treated by injecting pSUPER-shRNA/mdrl in situ and into intra- abdominal cavity. Tumors were collected to create cell suspension and cryosections after chemothearpy with adiramycin and mytomycin. The cell suspension was incubated in RPMI-1640 supplemented with G418 to screen stable cells for appreciating the reversal of MDR. Cryosections were treated with immunohistochemistry technique to show the effectiveness of transfection and the expression of ***： pSUPER-shRNA/mdrl was successfully constructed, which was confirmed by sequencing. The MDR phenotype of HepG2/mdr1 was decreased significantly in vitro transfection. HepG2/mdr1 showing its MDR was reversed notably in P-gp expression （11.0% vs 98.2%, P 〈 0.01）. Real-time PCR showed that mRNA/mdrl was lower in test groups than in control groups （18.73± 1.33 vs 68.03±2.21, P 〈 0.001）. Compared with HepG2, the sensitivity of HepG2/ mdrl and HepG2/mdr1-dsRNA cells to ADM was decreased by 1.64 times and 15.6 times, respectively. The accumulation of DNR in positive groups was decreased evidently. In vivo, the p-gp expression in positive groups was significantly lower than that in control groups （65.1% vs 94.1%, P 〈 0.05）. The tumor suppressing rate in test groups was 57.8%. After chemotherapy, the growth rate in test groups was lower than that in control groups （

关键词： Hepatocellular carcinoma Multi-drugresistance RNA interference

SGCS-made ultrafine CaO/Al_2O_3 sorbent for cyclic CO_2 capture

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Chinese Chemical Letters 2011年第5期22卷 615-618页

作者： Cong Luo Ying Zheng Ning Ding Qi Long Wu chu guang Zheng State Key Laboratory of Coal Combustion Huazhong University of Science and Technology Wuhan 430074 China

Multi-cyclic CaO carbonation/calcination is an attractive method for CO_2 capture during coal ***,the capture capacity of CaO sharply decreases with increasing carbonation/calcination *** order to improve the stability of CO_2 capture capacity of CaO during carbonation/calcination cycles,synthetic CaO/Al_2O_3 sorbents were synthesized by two methods：wet chemistry and sol-gel-combustion-synthesis（SGCS） to make a further *** results indicate that the SGCS-made CaO/ Al_2O_3 = 80：20 wt%sorbent provides a competitive performance of a capture capacity of 0.43 g-CO_2/g-sorbent after 20 cycles.

关键词： CO_2 capture CaO Carbonation/calcination cycles SGCS

EXTERNAL BODY FORCEIN FINITE DIFFERENCE LATTICE BOLTZMANN METHOD

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Journal of Hydrodynamics 2005年第4期17卷 473-477页

作者： CHEN Sheng LIU Zhao-hui SHI Bao-chang ZHENG chu-guang State Key Laboratory of Coal Combustion Huazhong University of Science and Technology Wuhan 430074 China

A new finite difference lattice Boltzmann scheme is developed. Because of analyzing the influence of external body force roundly, the correct Navier-Stokes equations with the external body force are recovered, without any additional unphysical terms. And some numerical results are presented. The result which close agreement with analytical data shows the good performance of the model.

关键词： Finite Difference Lattice Boltzmann Method (FDLBM) body force

Soil Nitrogen Distribution and Plant Nitrogen Utilization in Direct-Seeded Rice in Response to Deep Placement of Basal Fertilizer-Nitrogen

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Rice science 2019年第6期26卷 404-415页

作者： WANG Danying YE Chang XU chunmei WANG Zaiman CHEN Song chu guang ZHANG Xiufu State Key Laboratory of Rice Biology China National Rice Research Institute Hangzhou 310006 China Key Laboratory of Key Technology on Agricultural Machine and Equipment Ministry of Education / South China Agricultural University Guangzhou 510642 China

Deep placement of controlled-release fertilizer increases nitrogen (N) use efficiency in rice planting but is expensive. Few studies on direct-seeded rice have examined the effects of deep placement of conventional fertilizer. With prilled urea serving as N fertilizer, a two-year field experiment with two N rates (120 and 195 kg/hm2) and four basal N application treatments (B50, all fertilizer was broadcast with 50% as basal N;D50, D70 and D100 corresponded to 50%, 70% and 100% of N deeply placed as basal N, respectively) were conducted in direct-seeded rice in 2013 and 2014. Soil N distribution and plant N uptake were analyzed. The results showed that deep placement of basal N significantly increased total N concentrations in soil. Significantly greater soil N concentrations were observed in D100 compared with B50 at 0, 6 and 12 cm (lateral distance) from the fertilizer application point both at mid-tillering and heading stages. D100 presented the highest values of dry matter and N accumulation from seeding to mid-tillering stages, but it presented the lowest values from heading to maturity stages and the lowest grain yield for no sufficient N supply at the reproductive stage. The grain yield of D50 was the highest, however, no significant difference was observed in grain yield, N agronomic efficiency or N recovery efficiency between D70 and D50, or between D70 and B50, while D70 was more labor saving than D50 for only one topdressing was applied in D70 compared with twice in other treatments. The above results indicated that 70% of fertilizer-N deeply placed as a basal fertilizer and 30% of fertilizer-N topdressed as a panicle fertilizer constituted an ideal approach for direct-seeded rice. This recommendation was further verified through on-farm demonstration experiments in 2015, in which D70 produced in similar grain yields as B50 did.

关键词： direct-seeded rice nitrogen fertilizer deep placement soil nitrogen distribution nitrogen utilization

Subgrid-Scale Fluid Statistics along the Inertial Particle Trajectory in Isotropic Turbulence

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Chinese Physics Letters 2012年第9期29卷 93-96页

作者： YI Chao LI Jing LIU Zhao-Hui WANG Lin ZHENG chu-guang State Key Laboratory of Coal Combustion Huazhong University of Science and TechnologyWuhan 430074

Fluctuations of sub-grid scale(SGS)velocity and its influence on the motion of particles are important issues for large eddy simulation(LES)of gas-particle *** obtain the SGS statistics from the direct numerical simulation(DNS)data of homogeneous isotropic turbulent flow,which is filtered by the top filter in physical *** result shows that the SGS kinetic energy seen by particles reach a minimum value when the Stokes number St closes to ***,the turning point shifts towards a larger St as the filter width *** from that observed in DNS,the Lagrangian integral time scale is larger than the Eulerian integral time scale in *** small particles,the Lagrangian timescale of SGS fluid seen by particle is very close to that of the fluid *** particles which are large enough,it approaches the Eulerian timescale of SGS *** the intermediate particles,the predicted curve of SGS fluid timescale seen by particle varies with St and its variation is non-monotonic.

关键词： space. particle filtered