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mLife 2024年第1期3卷 143-155页

作者： Jing Zhang Qi-Yun Liang Da-Shuai Mu Fengbai Lian Ya Gong Mengqi Ye guan-jun chen Yuqi Ye Zong-jun Du State Key Laboratory of Microbial Techlogy Insttute of Micrbial TchnoogyShandong UniversityQingdaoChina Marine College Shandong UniverstyWeihaiChina Shandong University-Weihai Research Institute of Industrial Technology WeihaiChina

In the classical microbial isolation technique,the isolation process inevitably destroys all microbial interactions and thus makes it difficult to culture the many microorganisms that rely on these interactions for *** this study,we designed a simple coculture technique named the“sandwich agar plate method,”which maintains microbial interactions throughout the isolation and pure culture *** total yield of uncultured species in sandwich agar plates based on eight helper strains was almost 10-fold that of the control *** uncultured species displayed commensal *** study found that heme was the growth-promoting factor of some marine commensal *** genomic analysis revealed that heme auxotrophies were common in various biotopes and prevalent in many uncultured microbial ***,our study supported that the survival strategies of heme auxotrophy in different habitats varied *** findings highlight that cocultivation based on the“sandwich agar plate method”could be developed and used to isolate more uncultured bacteria.

关键词： cultivation growth factors heme sandwich agar plate uncultured bacteria

Strategies for culturing active/dormant marine microbes

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Marine Life Science & Technology 2021年第2期3卷 121-131页

作者： Da-Shuai Mu Yang Ouyang guan-jun chen Zong-jun Du State Key Laboratory of Microbial Technology Institute of Microbial TechnologyShandong UniversityQingdao266237China Marine College Shandong UniversityWeihai264209China Institute for Environmental Genomics University of OklahomaNormanOKUSA

Microorganisms are ubiquitous in the ocean environment and they play key roles in marine ecosystem function and ***,many of their functions and phenotypes remain unknown because indigenous marine bacteria are mostly difficult to *** many novel techniques have brought previously uncultured microbes into laboratory culture,there are still many most-wanted or key players that need to be cultured from marine *** review discusses possible reasons for‘unculturable microbes’and categorizes uncultured bacteria into three groups:dominant active bacteria,rare active bacteria,and dormant *** review also summarizes advances in cultivation techniques for culturing each group of unculturable *** the natural environment is an effective strategy for isolating dominant active bacteria,whereas culturomics and enrichment culture methods are proposed for isolating rare active *** dormant bacteria,resuscitation culture is an appropriate ***,the review provides a list of the most-wanted bacteria and proposes potential strategies for culturing these bacteria in marine *** review provides new insight into the development of strategies for the cultivation of specific groups of uncultured bacteria and therefore paves the way for the detection of novel microbes and their functions in marine ecosystems.

关键词： Uncultured marine bacteria Culture strategies Dominant active bacteria Rare active bacteria Dormant bacteria Most-wanted bacteria

Inflammation-based Glasgow prognostic score as an independent prognostic factor in patients with angioimmunoblastic T-cell lymphoma

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Chinese Medical Journal 2021年第5期134卷 579-581页

作者： guan-jun chen Zhi-jun Wuxiao Yang Liang Chun Li Bi-Bo Fu Hua Wang Department of Hematological Oncology Sun Yat-sen University Cancer CenterState Key Laboratory of Oncology in South ChinaCollaborative Innovation Center for Cancer MedicineGuangzhouGuangdong 510060China Department of Hematologic Oncology The First Affiliated HospitalHainan Medical UniversityHaikouHainan 570102China

Angioimmunoblastic T-cell lymphoma(AITL)is an aggressive form of peripheral T-cell lymphoma(PIT)associated with poor *** is characterized by lymph node enlargement,B symptoms(unexplained recurrent fevers(often above 38℃),night sweats,and unexplained weight loss of more than 10%within 6 months),polyclonal hypergammaglobulinemia,and autoimmune hemolysis.[1] C-reactive protein(CRP)synthesized by hepatocytes is an acute-phase protein and an important marker of systemic *** CRP level is not only an independent prognostic factor in Hodgkin lymphoma but also an important independent predictor of AITL.

关键词： lymphoma Glasgow blastic

Realizing the excellent oxidation resistance of an environmental barrier coating through aluminum surface modification

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Journal of Advanced Ceramics 2024年第7期13卷 976-986页

作者： Lin Dong Wen-Qi Yang Lin chen guan-jun Yang Chang-Jiu Li State Key Laboratory for Mechanical Behavior of Materials School of Materials Science and EngineeringXi’an Jiaotong UniversityXi’an 710049China Xi’an Aerospace Engine Company Limited in China Aerospace Science and Technology Corporation Xi’an 710065China

The lifetime of Si-based environmental barrier coatings(EBCs)is constrained by thermally grown SiO_(2)oxidelayer(SiO_(2)-TGO),which can cause premature cracking and *** address this issue,a new approach for surfacemodification using aluminum is *** oxidation performance was examined in a 50 vol%H_(2)O-50 vol%O_(2)environment at 1350℃for up to 300 *** results indicate that a dense ytterbium aluminum garnet(YbAG)layer wasformed after modification,decreasing the porosity by 80%.Due to the elimination of fast diffusion channels and the lowoxygen permeability of YbAG,aluminum modification significantly reduced the growth rate of SiO_(2)-TGO by nearly twoorders of ***,its thickness decreased by more than 70%after 300 h of exposure.A diffusioncontrolled oxidation mechanism indicates that the modified dense surface is equivalent to an initial SiO_(2)layer with a specificthickness,causing a shift in the oxidation time and increasing the oxidation *** research provides valuableinsights for designing Si-based EBC with improved lifetimes.

关键词： environmental barrier coating(EBC) oxidation modification diffusion cracking

Durable dual-state duplex Si-HfO_(2)with excellent oxidation and cracking resistance

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Journal of Advanced Ceramics 2024年第3期13卷 388-401页

作者： Lin chen Jing-Chuan Luo Wen-Qi Yang Chang-Jiu Li guan-jun Yang State Key Laboratory for Mechanical Behavior of Materials School of Materials Science and EngineeringXi’an Jiaotong UniversityXi’an 710049China Xi’an Aerospace Engine Company Limited in CASC Xi’an 710065China

The lifetime of Si bond coatings in environmental barrier coatings is constrained by phase-transition-induced cracking of the SiO_(2)*** this study,Si-HfO_(2)dual-state duplex composite materials are proposed to address this issue by partially forming HfSiO_(4)and minimizing the SiO_(2)*** as-prepared composite exhibited a structure comprising discrete HfO_(2)“bricks”embedded in a continuous Si“mortar”,while the oxidized state transformed into discrete HfSiO_(4)“bricks”within continuous thin SiO_(2)“mortars”.The results indicate that continuous thin SiO_(2)contributes to reducing the oxidation rate to a level comparable to that of pure Si,and discrete HfSiO_(4)particles aid in relieving phase transition-induced stress and inhibiting crack propagation,thereby enhancing oxidation and cracking resistance ***,the composite with 20 mol%HfO_(2)and a mean particle size of~500 nm at 1370℃exhibited a service lifetime 10 times greater than that of pure *** research provides valuable insights for designing Si-based bond coatings with improved service lifetime.

关键词： oxidation environmental barrier coatings(EBCs) phase transition cracking bond coatings

Short‑chain fatty acids(SCFAs)as potential resuscitation factors that promote the isolation and culture of uncultured bacteria in marine sediments

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Marine Life Science & Technology 2023年第3期5卷 400-414页

作者： Chun-Shui Sun Liu-Yan Zhou Qi-Yun Liang Xiao-Man Wang Yi-Xuan Lei Zhen-Xing Xu Feng-Qing Wang guan-jun chen Zong-jun Du Da-Shuai Mu Marine College Shandong UniversityWeihai 264209China State Key Laboratory of Microbial Technology Institute of Microbial TechnologyShandong UniversityQingdao 266237China Weihai Research Institute of Industrial Technology of Shandong University Weihai 264209China Institute of Microbiology Applications Xinjiang Academy of Agricultural SciencesUrumqi 830000China Tancheng County Inspection and Testing Center Tancheng 276100China Department of Applied Biological Chemistry Graduate School of Agricultural and Life SciencesThe University of TokyoBunkyo-KuTokyo 113-8657Japan Max Planck Institute for Marine Microbiology Celsiusstraße 128359 BremenGermany

Many marine bacteria are difcult to culture because they are dormant,rare or found in *** culturing has been widely tested as an important strategy to isolate rare or dormant ***,many more mechanisms remain ***,based on 16S rRNA gene high-throughput sequencing and metabolomics technology,it was found that the short-chain fatty acids(SCFAs)in metabolites were signifcantly correlated with uncultured bacterial groups during enrichment cultures.A pure culture analysis showed that the addition of SCFAs to media also resulted in high efciency for the isolation of uncultured strains from marine *** a result,238 strains belonging to 10 phyla,26 families and 82 species were successfully *** uncultured rare taxa within Chlorobi and Kiritimatiellaeota were successfully *** the newly isolated uncultured microbes,most genomes,***,possess SCFA oxidative degradation genes,and these features might aid these microbes in better adapting to the culture media.A further resuscitation analysis of a viable but non-culturable(VBNC)Marinilabiliales strain verifed that the addition of SCFAs could break the dormancy of Marinilabiliales in 5 days,and the growth curve test showed that the SCFAs could shorten the lag phase and increase the growth ***,this study provides new insights into SCFAs,which were frst studied as resuscitation factors in uncultured marine ***,this study can help improve the utilisation and excavation of marine microbial resources,especially for the most-wanted or key players.

关键词： Marine bacteria SCFAs VBNC Resuscitation culture

Efficient removal of Cr(VI) by polydopamine-modified lignin from aqueous solution: Batch and XAFS studies

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Water Science and Engineering 2024年第1期17卷 51-61页

作者： Xiao-jie Guo Wei-kang Fu Jing-yuan Ma Bo-jun Xi chen Wang Meng-yao guan College of Materials and Environmental Engineering Hangzhou Dianzi UniversityHangzhou 310018China College of Textile and Garment Shaoxing UniversityShaoxing 312000China Shanghai Synchrotron Radiation Facility Shanghai Advanced Research InstituteChinese Academy of SciencesShanghai 201204China

Lignocellulose has the potential to become a bio-based adsorbent due to its biodegradability and *** this study,a novel polydopamine functionalized-lignin(lignin@PDA),prepared via self-polymerization of dopamine(PDA)on lignin,was used as a bio-based adsorbent for rapid scavenging of hexavalent chromium(Cr(VI)).The morphology,functional groups,crystalline structure,and chemical composition of lignin@PDA were characterized with a scanning electron microscope-energy dispersive spectrometer,Fourier transform infrared spectroscopy,X-ray diffraction,and X-ray photoelectron *** Cr(VI)adsorption process of lignin@PDA was studied using batch experiments as a function of pH,ionic strength,adsorbent dose,and contact time at room *** adsorption rate of lignin@PDA was five times greater than that of the unmodified lignin,with a maximum adsorption capacity of 102.6 mg/g in an acidic *** adsorption of Cr(VI)on lignin@PDA fit the pseudo-secondorder equation and the Freundlich model,indicating that the adsorption process was mainly dominated by chemisorption and surface *** thermodynamic parameters showed that adsorption of Cr(VI)on lignin@PDA was an endothermic and spontaneous *** X-ray absorption fine structure results showed that sorption and reduction of Cr(VI)into Cr(II)occurred simultaneously on ***,PDA coating not only improved the reactivity of lignin but also promoted the complete reduction of Cr(VI)by *** to these results,polydopamine functionalized-lignin is a promising bio-based adsorbent for immobilization of Cr(VI)from wastewater.

关键词： Lignin Polydopamine Hexavalent chromium EXAFS Adsorption mechanism

Dynamic Couplingand Cooperative Control for Multi-paralleled Doubly Fed Induction Generator Wind Farms during Symmetrical Low Voltage Ride-through in a Weak Grid

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Protection and Control of Modern Power Systems 2024年第3期9卷 112-125页

作者： Lei guan jun Yao the College of Electrical Engineering at Chongqing University ChongqingChina the State Key Laboratory of Power Transmission Equipment Technology School of Electrical EngineeringChongqing UniversityChongqing 400044China

In multi-fed grid-connected systems,there are complex dynamic interactions between different pieces of *** in situations of weak-grid faults,the dynamic coupling between equipment becomes more *** may cause the system to experience small-signal instability during the fault *** this paper,multi-paralleled doubly fed induction generator(DFIG)-based wind farms(WFs)are taken as an example to study the dynamic coupling within a multi-fed system during fault steady-state of symmetrical low voltage ride-through(LVRT)in a weak *** analysis reveals that the dynamic coupling between WFs will introduce a damping shift to each *** inevitably affects the system’s dynamic stability and brings the risk of small-signal instability during fault steady-state in LVRT *** the distance to fault location and fault severity will exacerbate the dynamic coupling between *** of the dynamic coupling,adjusting the control state of one WF will affect the stability of the remaining WFs in the ***,a cooperative control strategy for multi-paralleled DFIG WFs is proposed to improve dynamic stability during *** analysis and the effectiveness of the proposed control strategy are verified by modal analysis and simu-lation.

关键词： Doubly fed induction generator(DFIG) wind farms(WFs) dynamic stability low voltage ride through(LVRT) weak grid dynamic coupling

Emotional agent based on rough set

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通讯和计算机（中英文版） 2008年第5期5卷 16-20页

作者： HU jun guan Chun chen Yu-hai School of Software Nanchang University Nanchang 330047 China

To improve the ability of agent for handling emotions, a new architecture for emotion agent is presented, based on the traditional BDI agent model and the rough set theory. By a new emotional reasoning algorithm based on rough set, the architecture performs emotion reasoning and implements the emotion treatment. An emotional agent grid based on this architecture has been realized, experiment results show that it is efficient to transact simple emotions by emotional agents.

关键词：粗糙集移动主体体系结构计算机技术

Photothermal catalysts for efficient and clean fuel production at atmospheric pressure

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Science China Chemistry 2024年第6期67卷 1762-1764页

作者： Xinwei guan Yang chen Tianyi Ma School of Science RMIT UniversityMelbourneVIC3000Australia Institute of Clean Energy Chemistry Key Laboratory for Green Synthesis and Preparative Chemistry of Advanced Materials of Liaoning ProvinceCollege of ChemistryLiaoning UniversityShenyang110036China

The use of waste carbon resources to produce valuable chemicals and hydrocarbon fuels has attracted great interest in recent *** a transformation not only maximises the utilisation of carbon resources but also plays a... 详细信息

关键词： thermal transformation clean