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Testosterone replacement maintains smooth muscle content in the corpus cavernosum of orchiectomized rats

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Asian Journal of Urology 2017年第4期4卷 223-229页

作者： graziele Halmenschlager Ernani Luis Rhoden Gabriela Almeida Motta Lucas Sagrillo Fagundes Jorge Luiz Medeiros Jr Rosalva Meurer Claudia Ramos Rhoden Centro Universitario Ritter dos Reis(UniRitter)and Universidade Federal de Cieˆncias da Sau´de de Porto Alegre(UFCSPA)e PPG Cieˆncias da Sau´de Porto AlegreRSBrazil Department of Urology at Universidade Federal de Cieˆncias da Sau´de de Porto Alegre(UFCSPA) Urologist at Irmandade Santa Casa de Miserico´rdia de Porto Alegre(ISCMPA)and Hospital Moinhos de Vento de Porto Alegre(HMV)Porto AlegreRSBrazil Universidade Federal de Cieˆncias da Sau´de de Porto Alegre(UFCSPA)e PPG Cieˆncias da Sau´de-Porto Alegre RSBrazil INRS Institut Armand Frappier(Universit.du Que´bec)and BioMed Research Center Laval QuebecCanada Laboratory of Morphometry Metabolismand Cardiovascular DiseaseBiomedical CenterInstitute of BiologyState University of Rio de JaneiroRio de JaneiroBrazil Laboratory of Multidisciplinary of Sciences Biomedical CenterMedical School of Valenc¸aValenc¸a/Rio de JaneiroBrazil

Objective:To evaluate the effects of testosterone(T)on the maintenance of corpus cavernosum(CC)structure and ***:Animals were divided into three groups:sham operation group(n Z 8)underwent sham operation;Orchiectomized(Orchiec)t oily vehicle group(n Z 8)underwent bilateral orchiectomy and received a single dose of oily vehicle by intramuscular injection(i.m.)30 days after orchiectomy;and Orchiec t T group(n Z 8)underwent bilateral orchiectomy and received a single dose of T undecanoate 100 mg/kg i.m.30 days after the *** were euthanized 60 days after the beginning of the experiment with an anesthetic overdose of ketamine and *** samples and penile tissue were collected on euthanasia.

关键词： Testosterone replacement Histomorphometry Androgen deprivation Hypogonadism Corpus cavernosum

Distinguishing the Regional Atmospheric Controls on Precipitation Isotopic Variability in the Central-Southeast Portion of Brazil

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Advances in Atmospheric Sciences 2022年第10期39卷 1693-1708,I0018-I0020页

作者： Vinícius dos SANTOS Peter MARSHALL FLEMING Luís HENRIQUE MANCINI Stela DALVA SANTOS COTA graziele Beatriz de LIMA Rafaela RODRIGUES GOMES Roberto Eduardo KIRCHHEIM Ricardo SANCHÉZ-MURILLO Didier GASTMANS São Paulo State University(UNESP) Environmental Studies Center.Av.24ABased1515-Bela Vista13.506-900Rio Claro(SP)Brazil Center for the Development of the Nuclear Technology(CDTN-CNEN) Av.Presidente Antônio Carlos6.627Campus da UFMG-Pampulha31270-90Belo Horizonte(MG)Brazil Universidade de Brasília-UnB Instituto de GeociênciasLaboratório de Isótopos Estáveis.Campus"Darcy Ribeiro"Asa Norte70.910-900Brasília(DF)Brasil Hydrology and Territorial Management Directory(DHT) The Geological Survey of Brazil(CPRM-SGB)São Paulo(SP)Brazil University of Texas at Arlington Earth and Environmental Sciences Department500 Yates StreetArlingtonTexas76019USA Stable Isotopes Research Group and Water Resources Management Laboratory Universidad NacionalHerediaCosta Rica

Precipitation isotope ratios(O and H)record the history of water phase transitions and fractionation processes during moisture transport and rainfall ***,we evaluated the isotopic composition of precipitation over the central-southeastern region of Brazil at different *** isotopic compositions were associated with classical effects(rainfall amount,seasonality,and continentality),demonstrating the importance of vapor recirculation processes and different regional atmospheric systems(South American Convergence Zone-SACZ and Cold Fronts-CF).While moisture recycling and regional atmospheric processes may also be observed on a daily timescale,classical effects such as the amount effect were not strongly correlated(δ^(18)O-precipitation rate r≤-0.37).Daily variability revealed specific climatic features,such asδ^(18)O depleted values(~-6‰to-8‰)during the wet season were associated with strong convective activity and large moisture *** isotopic analysis revealed the role of different moisture sources and transport *** ratios combined with d-excess explain how atmospheric recirculation processes interact with convective activity during rainfall formation *** findings provide a new understanding of rainfall sampling timescales and highlight the importance of water isotopes to decipher key hydrometeorological processes in a complex spatial and temporal context in central-southeastern Brazil.

关键词： precipitation stable water isotopes d-excess moisture source and transport southern Atlantic Ocean and Amazon

Family visits to a biodiversity exhibit: An analysis of emotional responses during free visits and visits mediated by explainers

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Cultures of Science 2023年第2期6卷 214-234页

作者： Luisa Massarani graziele Scalfi Rosicler Neves Monica Santos Dahmouche Luiz Bento National Institute of Public Communication of Science and Technology (INCT-CPCT) National Institute of Public Communication of Science Technology(INCT-CPCT) Museum of Life House of Oswaldo Cruz/Fiocruz National Institute of Public Communication of Science and Technology (INCT-CPCT) CECIERJ Foundation

In this study, we analyse the role of emotion in the context of informal science education experiences, specifically seeking to understand:(a) which emotions can be stimulated in families visiting interactive expositions, and(b) the extent to which parents/caregivers and museum explainers engage the children's emotional expressiveness. To this end, a protocol based on the core affect model was employed to examine the visits of 10 family groups(26 individuals) who visited an exhibit on biodiversity in a science museum in Rio de Janeiro, Brazil. Five families went on free visits and the other five had their visits mediated by explainers. We observed that, in general, positive emotions, such as excitement and surprise, were among those most often expressed. Negative emotions, such as disagreement and doubt, also stood out in this study, but they were not exclusively associated with the negative aspects of the experience, and were instead linked to challenging moments that facilitated the construction of meaning by the families. The data suggest that visits mediated by explainers occasioned more frequent emotional displays than free visits, indicating the importance of explainers in families' interactions and emotional responses.

关键词： Science museums emotions family visit exhibit explainers conversations

Retrieving dental instruments through endoscopy: A literature review

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World Journal of Stomatology 2015年第4期4卷 137-140页

作者： Rhonan Ferreira Silva Ademir Franco Fernando Fortes Picoli Mayara Barbosa Viandelli Mundim Livia graziele Rodrigues Depart-ment of Stomatological Sciences Forensic Odontology Federal University of Goiás Depart-ment of Forensic Odontology and Anthropology Scientific Police of Goiás Department of Stomatology Pontifícia Univer-sidade Católica do Paraná

Clinical accidents involving dental instruments and materials inside the oral cavity are reported in the medical literature. Specifically, ingestion and aspiration of foreign bodies have greater prevalence in the routine of medicine and dentistry. Despite being less harmful than aspirations, the accidental ingestion of dental instruments does not always culminate in favorable prognoses. Mostly, complex conditions require medical intervention through endoscopy or surgical approaches. The present research aims to review the literature pointing out the specialties of dentistry most involved with accidental ingestion of dental instruments, highlighting the important role of endoscopy for accurately locating and retrieving foreign bodies. Prosthodontics, operative dentistry, orthodontics, and maxillofacial surgery arose as the specialties in which these accidents are more prevalent. Based on that, general dentists and specialists must be aware for the essential care to avoid such clinical accidents, as well as to know the available tools, such as endoscopy, to overcome these situations in the routine of dentistry.

关键词： Endoscopy Accidents Dental instruments Foreign bodies

NiO-CGO Composite for SOFC Anode： Synthesis and Characterization

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材料科学与工程（中英文版） 2010年第5期4卷 33-39页

作者： Beatriz Cela Daniel Araujo de Macedol graziele Lopes de Souza Auristela Carla de Miranda Antonio Eduardo Martine Rubens Maribondo do Nascimento Carlos Alberto Paskocimas PPGCEM CCET Federal University of Rio Grande do Norte 1524 - 59072-970 Natal RN Brazil Chemical Engineering Department CT Federal University of Rio Grande do Norte Natal 1524 - 59072-970 RN Brazil

New Technique to Produce Large Amount of Flat Silk by Biospinning

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Agricultural Sciences 2014年第14期5卷 1483-1490页

作者： Laura Beatriz Garay Andrea Nembri Alana Lucia Oro Veronica Aureliana Fassina Claudia Regina das Neves Saez Alex Sandro Chiarello Naiara Climas Pereira graziele Milani Pessini Roxelle Ethienne Ferreira Munhoz Maria Aparecida Fernandez Seda y Fibras S.R.L HernandariasParaguay Departamento de Biotecnologia Genetica e Biologia CelularUniversidade Estadual de MaringaMaringaBrasil

Beyond the production of silk thread, there are several studies showing that the silk is a great biomaterial for surgical sutures and grafts. This paper shows a new technique to produce silk thread changing the natural cycle of silk production, which is the production of cocoons. This new method has the purpose of producing a silk fabric free of impurities, through flat surfaces. Six different surfaces were tested: Glass, Formica Surface, Steel and Zinc Sheets, Cotton tissue and Burlap Bag. The first five surfaces had not presented enough larvae alive for statistical analysis, because there were several damages in silkworms larvae that resulted in mortality and low silk production. On the other hand, the burlap bag surface presented good results for web construction by biospinning and its use was indicated for silk industry focused on biomaterials. The present study suggested the potential of naturally biospun web, using Bombyx mori, to develop a new technique to produce silk thread matrices that will have several applications at the industry and production of biomedical materials.

关键词： Silkworm Bombyx mori L. Silk Thread Biomaterial

Low Genetic Polymorphism at the Cytochrome C Oxidase I in Silkworm Strains of the Brazilian Germplasm Bank

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Open Journal of Genetics 2014年第3期4卷 202-209页

作者： Veronica Aureliana Fassina Thais Souto Bignotto Roxelle Ethienne Ferreira Munhoz Bruno Fulan Juliana Pereira Bravo Laura B.Garay Rafaela Bespalhuk Claudia Regina das Neves Saez Naiara Climas Pereira graziele Milani Pessini Maria Aparecida Fernandez Departamento de Biotecnologia Genetica e Biologia CelularUniversidade Estadual de MaringaUEMParanaBrasil Seda y Fibras S.R.L. HernandariasParaguay

Nucleotide sequences have been used to distinguish species and specimens for many years. More recently, the use of a partial sequence of 650 bp of the cytochrome c oxidase I, COI mitochondrial gene, has been proposed for species identification, known as DNA barcodes. In this work, a short sequence of the DNA barcode is described—approximately 250 bp, named as “DNA mini-barcode”—to molecularly identify different silkworm strains maintained at the unique public Germplasm Bank of Bombyx mori, at the Universidade Estadual de Maringá, UEM, Brazil. Analysis revealed no significant differences among the silkworm strains. The phylogenetic tree obtained by the neighbor-joining method and K2P distance, in which specimens of B. mandarina were used as outgroup, clustered all the specimens of B. mori in a unique clade. Genetic variability detect within B. mori was low or nonexistent. In conclusion, the partial region of 250 bp of the mitochondrial gene COI herein analyzed may not be efficient to discriminate silkworm strains from the UEM Germplasm Bank of Bombyx mori.

关键词： Bombyx mori Mitochondrial Gene COI DNA Mini-Barcode

Detection of Contamination and Analysis of Vertical Transmission of BmNPV in Eggs and Moths of Bombyx mori

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Open Journal of Genetics 2014年第5期4卷 370-377页

作者： Claudia Regina das Neves Saez Roxelle Ethienne Ferreira Munhoz Naiara Climas Pereira Thais Souto Bignotto Veronica Aureliana Fassina graziele Milani Pessini Laura Beatriz Garay Lucineia de Fatima Chasko Ribeiro Rose Meire Costa Brancalhao Maria Aparecida Fernandez Departamento de Biotecnologia Genetica e Biologia CelularUniversidade Estadual de MaringaMaringaBrasil Seda y Fibras HernandariasParaguay Universidade Estadual do Oeste do Parana CascavelBrasil

This study reports the molecular detection of Bombyx mori nucleopolyhedrovirus (BmNPV) in silkworm strains of the Universidade Estadual de Maringá Brazilian Germplasm Bank (UBGB). DNA extraction was carried out by using six Bombyx mori female moths of each strain, followed by PCR amplification. A pair of primers was designed based on a specific sequence of the baculovirus genome related to the BmNPV ORF 14. Another pair of primers was used to amplify the silkworm Actin A3 gene segment, which was used as positive control. Twenty gene pools were analyzed, and fifteen revealed a fragment of 443 base pairs (bp), which indicated the presence of the BmNPV. The frequency of contaminated moths was as following: 100% for silkworm strains M18-2, M12-2 and J1;83% for C25, C75 and C24 strains;66% for KR01;50% for M11-A;33% for AS3, B106, M8 and M11 and 16% for C211, E8 and Hindu strains. These are promising results for the identification of contaminated B. mori moths by BmNPV, which may prevent virus proliferation in subsequent generations. We also analyzed DNA samples extracted from B. mori eggs, but the results were not conclusive regarding the detection of the fragments of the expected size (443 bp). The difficulty in detecting BmNPV contamination in B. mori eggs may be due to the low concentration of virus in samples.

关键词： Silkworm Diseases Sericulture Viruses in Insects BmNPV Bombyx mori

Evaluation of the toxic effect of insecticide chlorantraniliprole on the silkworm Bombyx mori(Lepidoptera:Bombycidae)

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Open Journal of Animal Sciences 2013年第4期3卷 343-353页

作者： Roxelle Ethienne Ferreira Munhoz Thais Souto Bignotto Naiara Climas Pereira Claudia Regina das Neves Saez Rafaela Bespalhuk Veronica Aureliana Fassina graziele Milani Pessini Mayarha Patricia Dequigiovanni Baggio Lucineia de Fatima Chasko Ribeiro Rose Meire Costa Brancalhao Shunsuke Mizuno Willian Shigeaki Aita Maria Aparecida Fernandez Departamento de Biotecnologia Genetica e Biologia CelularUniversidade Estadual de MaringaMaringaBrasil Universidade Estadual do Oeste do Parana CascavelBrasil Fiacao de Seda Bratac S.A.Bastos Sao PauloBrasil

The silkworm Bombyx mori feeds exclusively on mulberry leaves and is highly sensitive to pesticides in general. Although mulberry plantations are free of agrochemicals, pesticide drift can occur. Chlorantraniliprole, a novel insecticide of the anthranilic diamides class, has been used to control pests in field crops. In this study, we investigated the biological effects of different concentrations of chlorantraniliprole on B. mori silkworm commercial Brazilian hybrids. To evaluate the toxicity of chlorantraniliprole, bioassays were carried out and data on the lethal concentrations, symptomatology, morphology and variables of silk production were collected. Results indicated that B. mori is extremely sensitive to chlorantraniliprole, even in low concentrations. The highest silkworm mortality rates were observed in the two highest chlorantraniliprole concentrations, 0.2 and 0.1 ppm. Although lower chlorantraniliprole concentrations did not cause death of all the silkworm larvae, various symptoms of toxicity were observed: feeding cessation, regurgitation, late development and incomplete ecdysis. Such symptoms reflect the morphological changes we observed in the midgut epithelium, which affected nutrient uptake and metabolism, and even the production of cocoons. Exposed larvae also produced?thin-shelled cocoons, which constitutes a serious economic problem because this type of cocoon is not useful for the silk industry. The results provided herein confirm the toxicity of chlorantraniliprole in silkworm larvae. Therefore, we strongly suggest that, competent authorities of the National Health Surveillance Agency, in pesticide management should take measures to reduce or eliminate the use of chlorantraniliprole in areas nearest to silkworm cultivation.

关键词： Silkworm Pesticide Drift Mortality Symptomatology Chlorantraniliprole