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Effect of Loss-of-function of the Herpes Simplex Virus-1 microRNA H6-5p on Virus Replication

Virologica Sinica 2019年第4期34卷 386-396页

作者： Rongquan Huang Xusha zhou Shuqi Ren Xianjie Liu Zhiyuan Han Grace Guoying Zhou School of Basic Medical Sciences Guangzhou Medical UniversityGuangzhou 511436China Shenzhen International Institute for Biomedical Research Shenzhen 518116China

To date, 29 distinct microRNAs(miRNAs) have been reported to be expressed during herpes simplex virus infections.Sequence analysis of mature herpes simplex virus-1(HSV-1) miRNAs revealed five sets of miRNAs that are complementary to each other: miR-H6-5p/H1-3p, miR-H6-3p/H1-5p, H2-5p/H14-3p, miR-H2-3p/H14-5p, and miR-H7/H27.However, the roles of individual miRNAs and consequences of this complementarity remain unclear. Here, we focus on two of these complementary miRNAs, miR-H6-5p and miR-H1-3p, using loss-of-function experiments in vitro and in a mouse model of infection using an miRNA sponge approach, including tandem multiplex artificial miRNA-binding sequences that do not match perfectly to the target miRNA inserted downstream of a green fluorescent protein reporter gene. Infection with recombinant virus expressing the miR-H6-5p sponge reduced viral protein levels and virus yield.Decreased accumulation of viral proteins was also observed at early stages of infection in the presence of both an miR-H6-5p inhibitor and plasmid-expressed miR-H1-3p. Moreover, establishment of latency and reactivation did not differ between the recombinant virus expressing the miR-H6-5p sponge and wild-type HSV-1. Taken together, these data suggest that miR-H6-5p has an as-yet-unidentified role in the early stages of viral infection, and its complement miR-H1-3p suppresses this role in later stages of infection. This report extends understanding of the roles of miRNAs in infection by herpes simplex viruses, supporting a model of infection in which the production of virus and its virulent effects are tightly controlled to maximize persistence in the host and population.

关键词： miR-H6-5p miR-H1-3p Sponge Replication Herpes simplex virus-1(HSV-1)

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Termination of Transcription of LAT Increases the Amounts of ICP0 mRNA but Does Not Alter the Course of HSV-1 Infection in Latently Infected Murine Ganglia

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Virologica Sinica 2021年第2期36卷 264-272页

作者： Haifang Jiang Jiaming Wu Xianjie Liu Ruitao Lu Manling zhou Meiling Chen Yonghong Liu Grace Guoying Zhou Wenmin Fu School of Basic Medical Sciences Guangzhou Medical UniversityGuangzhou 511436China Shenzhen International Institute for Biomedical Research Shenzhen 518116China

On entering sensory ganglia,herpes simplex viruses 1(HSV-1)establishes a latent infection with the synthesis of a latency associated transcript(LAT)or initiates productive infection with expression of a set of immediate early viral proteins.The precise mechanisms how expression of a genes is suppressed during the latency are unknown.One mechanism that has been proposed is illustrated in the case of ICP0,a key immediate early viral regulatory protein.Specifically,the 2 kb LAT intron is complementary to the 30 terminal portion of ICP0 m RNA.To test the hypothesis that accumulation of LAT negatively affects the accumulation of ICP0 m RNA,we inserted a DNA fragment encoding two poly(A)sequences into LAT to early terminate LAT transcript without interrupting the complementary sequence of ICP0 transcript(named as SR1603).Comparisons of the parent(SR1601)and mutant(SR1603)HSV-1 viruses showed the following:Neurons harboring latent SR1603 virus accumulated equivalent amounts of viral DNA but higher amounts of ICP0 m RNA and lower amounts of LAT,when compared to neurons harboring the SR1601 virus.One notable difference between the two viruses is that viral RNA accumulation in explanted ganglia harboring SR1603 virus initiated significantly sooner than that in neurons harboring SR1601 virus,suggesting that ICP0 may act as an activator of viral gene expression in permissive cells.Collectively,these data suggest that increased ICP0 m RNA by suppressed LAT did not affect the establishment of latency in latently infected murine ganglia.

关键词： Herpes simplex viruses 1(HSV-1) Latency associated transcript(LAT) ICP0 Latency

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Structures of the portal vertex reveal essential protein-protein interactions for Herpesvirus assembly and maturation

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Protein & Cell 2020年第5期11卷 366-373页

作者： Nan Wang Wenyuan Chen Ling Zhu Dongjie Zhu Rui Feng Jialing Wang Bin Zhu Xinzheng Zhang Xiaoqing Chen Xianjie Liu Runbin Yan Dongyao Ni Grace Guoying Zhou Hongrong Liu Zihe Rao Xiangxi Wang CAS Key Laboratory of Infection and Immunity National Laboratory of MacromoleculesInstitute of BiophysicsChinese Academy of SciencesBeijing 100101China Key Laboratory for Matter Microstructure and Function of Hunan Province Key Laboratory of Low-dimensional Quantum Structures and Quantum ControlSchool of Physics and ElectronicsHunan Normal UniversityChangsha 410081China Laboratory of Structural Biology Tsinghua UniversityBeijing 100084China State Key Laboratory of Medicinal Chemical Biology Nankai UniversityTianjin 300353China ImmVira Co. LtdSilver Star Hi-tech Industrial ParkLonghua DistrictShenzhen 518116China

Dear Editor,Herpesviridae is a large family of double-stranded DNA(dsDNA)viruses that cause a variety of human diseases ranging from cold sores and chicken pox to congenital defects,blindness and cancer(Chayavichitsilp et al.,2009;Wang et al.,2018).In the past 70 years,substantial advances in our knowledge of the molecular biology of herpesviruses have led to insights into disease pathogenesis and management.However,the mechanism for capsid assembly that requires the ordered packing of about 4,000 protein subunits into the hexons,pentons and triplexes remains elusive.It is still a puzzle how initially identical subunits adopt both hexameric and pentameric conformations in the capsid and select the correct locations needed to form closed shells of the proper size.Biochemical and genetic studies have shown that the portal is involved in initiation of capsid assembly(Newcomb et al.,2005)and functions akin to a DNA-sensor coupling genome-packaging achieved by a genome-packaging machinery-“terminase complex”(Chen et al.,2020;Yunxiang Yang,2020)with icosahedral capsid maturation(Lokareddy et al.,2017).Structural investigations of the herpesvirus portal have proven challenging due to the small size of this dodecamer,which accounts for less than 1%of the total mass of the capsid protein layer and the technical difficulties involved in resolving non-icosahedral components of such large icosahedral viruses(diameter is∼1,250Å).Efforts of many investigators over two decades have made to reconstruct the cryo-electron microscopy(cryo-EM)structure of herpesvirus portal vertex and more recently near-atomic structures of two herpesvirus(herpes simplex virus type 1(HSV-1)and Kaposi’s sarcoma-associated herpesvirus(KSHV))portal vertices were reported(McElwee et al.,2018;Gong et al.,2019;Liu et al.,2019).

关键词： vertex shells packing

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Deletion of the waaf gene affects O antigen synthesis and pathogenicity in Vibrio parahaemolyticus from shellfish

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Food Science and Human Wellness 2022年第2期11卷 418-426页

作者： Feng Zhao guoying Ding Qilong Wang Huihui Du Guosheng Xiao Deqing zhou College of biology and food engineering Chongqing Three Gorges UniversityWanzhou 404100China Yellow Sea Fisheries Research Institute Chinese Academy of Fishery SciencesLaboratory for Marine Drugs and Bioproducts of Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266071China

Vibrio parahaemolyticus is the main cause of foodborne gastroenteritis,which is widely distributed in shellfish and other seafood.Most V.parahaemolyticus are nonpathogenic,and only a few types,such as serotype O3:K6,are pathogenic,which is also the most prevalent strain in Asia.However,the relationship between this serotype and pathogenicity has yet to be established.The waaf gene is located in the O antigen synthesis gene cluster.Thus,we constructed a waaf gene deletion mutant(i.e.,Δwaaf)of wild-type(WT)which isolated from shellfish serotype O3:K6 via chitin-mediated transformation technology.We then constructed theΔwaaf complementary strain(i.e.,C-△waaf)via the Escherichia coli S17λpir strain by conjugation.The basic physiological characteristics,adhesion to Caco2 cells,and pathogenicity of the WT,△waaf,and C-△waaf strains were compared.Growth curves showed no remarkable differences between the WT andΔwaaf strains.However,theΔwaaf strain non-reactive to O3 antisera and other 12 O-group antisera of V.parahaemolyticus.Moreover,the number of flgella and extracellular polysaccharides decreased,the adhesion decreased,and the pathogenicity weakened.These characteristics of the C-△waaf strain were similar to those of the WT strain These results indicated that the waaf gene is vital to the serotype in V.parahaemolyticus,and changes in O antigen could affect the pathogenicity of this bacterium.This study will be helpful to understand the pathogenic mechanism of V.parahaemolyticus.

关键词： Vibrio parahaemolyticus waaf gene LPS O antigen Pathogenicity

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Study of pre-processing model of coal-mine hoist wire-rope fatigue damage signal

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International Journal of Mining Science and Technology 2015年第6期25卷 1017-1021页

作者： Tian Jie Wang Hongyao zhou Junying Meng guoying School of Mechanical Electronic and Information Engineering China University of Mining and Technology

In this paper, we propose a pre-processing method for the detection of wire-rope signals. This is necessary because of the lack of processing methods that are currently employed. First, we investigated the one-dimensional discrete morphological and wavelet transform. Then, we developed a pre-processing model that is based on the morphological wavelet-filtering algorithm. We then proposed a modified morphology filtering algorithm. We also designed an experiment platform for wire-rope detection.Eight levels of localized flaws(LFs) and damage were formed in the wire-rope specimen. We performed a series of experimental studies, and the results show that the proposed method can effectively filter the drift signal. The signal-to-noise ratio of the new filtering algorithm was over 26 d B. The signal-to-noise ratio of the existing method is less than 15 d B, and the noise-signal ratio of the new filtering algorithm has improved by 73%. Based on our results, the filtering effect of the proposed method is better than that of the present method. This study has great significance and practical value in engineering applications.

关键词：信号预处理提升钢丝绳疲劳损伤模型形态滤波算法煤矿小波变换实验平台

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Three-dimensional boron nitride network/polyvinyl alcohol composite hydrogel with solid-liquid interpenetrating heat conduction network for thermal management

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Journal of Materials Science & Technology 2022年第32期127卷 183-191页

作者： Mengmeng Qin Yajie Huo guoying Han Junwei Yue Xueying zhou Yiyu Feng Wei Feng School of Materials Science and Engineering Tianjin UniversityTianjin 300072China Key Laboratory of Advanced Ceramics and Machining Technology Ministry of EducationTianjin 300072China School of Materials Science and Engineering Tianjin University of TechnologyTianjin 300084China

Polyvinyl alcohol hydrogels have been used in wearable devices due to their good flexibility and biocompatibility.However,due to the low thermal conductivity(κ)of pure hydrogel,its further application in high power devices is limited.To solve this problem,melamine sponge(MS)was used as the skeleton to wrap boron nitride nanosheets(BNNS)through repeated layering assembly,successfully preparing a three-dimensional(3D)boron nitride network(BNNS@MS),and PVA hydrogels were formed in the pores of the network.Due to the existence of the continuous phonon conduction network,the BNNS@MS/PVA exhibited an improvedκ.When the content of BNNS is about 6 wt.%,κof the hydrogel was increased to 1.12 W m^(-1)K^(-1),about two times higher than that of pure hydrogel.The solid heat conduction network and liquid convection network cooperate to achieve good thermal management ability.Combined with its high specific heat capacity,the composites have an important application prospect in the field of wearable flexible electronic thermal management.

关键词： Thermal conductivity Polyvinyl alcohol Three-dimensional network Composite hydrogel

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青藏高原高寒草原植物群落对降雨的响应依赖于优势种的功能性状

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Journal of Plant Ecology 2021年第4期14卷 569-579页

作者： Zhi Zheng Yue Zhang Shihu Zhang Qun Ma Dajie Gong guoying zhou College of Life Science Northwest Normal UniversityLanzhou 730070China Key Laboratory of Tibetan Medicine Research Northwest Institute of Plateau BiologyChinese Academy of ScienceXining 810008China

人类活动和全球变化引起了全球和区域降雨格局的改变。大量的研究表明,降雨影响全球不同草地生态系统的群落组成,但目前很少有研究从物种水平揭示高寒草原植物群落如何响应降雨变化的生态学过程。本研究利用在青藏高原建立的长期(6年)... 详细信息

人类活动和全球变化引起了全球和区域降雨格局的改变。大量的研究表明,降雨影响全球不同草地生态系统的群落组成,但目前很少有研究从物种水平揭示高寒草原植物群落如何响应降雨变化的生态学过程。本研究利用在青藏高原建立的长期(6年)控雨试验平台,包括减少降雨50%、增加降雨50%和对照3个处理,从优势种地上和地下性状揭示高寒草原植物群落响应降雨变化的生态学过程。研究结果表明,长期减少降雨改变了植物群落的结构,主要体现在优势种丛生禾草紫花针茅(Stipa purpurea)逐渐被根茎禾草赖草(Leymus secalinus)所取代。进一步通过比较紫花针茅和赖草的地上和地下性状,发现减少降雨主要促进了赖草根系向深土层的生长、增加了地下碳的分配和减少了叶片宽度,而紫花针茅的这些性状并没有发生显著的改变;另外,减少降雨增加了赖草根茎生物量、总长度、直径及其节上根的数目。赖草这些性状的改变可能增加了对深土层水分的利用能力,以及减少了水分的蒸腾,从而增强了其在干旱胁迫过程中的竞争力。因此,我们的研究结果强调了优势种的地上和地下性状在高寒草原植物群落组成对降雨变化响应过程中起着极其重要的作用。

关键词：降雨变化功能群优势种紫花针茅(Stipa purpurea) 赖草(Leymus secalinus) 土壤湿度植物性状高寒草原青藏高原

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液体聚硫橡胶的改性及应用进展

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广东化工 2023年第15期50卷 98-100页

作者：周国营国家知识产权局专利局专利审查协作北京中心北京100160

液体聚硫橡胶发展至今仍然是重要的特种橡胶之一,为了更好的使用此种橡胶,研究人员不断地致力于对其进行分子改性和应用领域的拓展。本文主要从分子改性和应用领域两方面进行了梳理总结,并展望了聚硫橡胶未来的发展。

关键词：聚硫橡胶改性端基密封剂

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磁流变弹性体相关专利分析

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广东化工 2023年第17期50卷 103-105,132页

作者：周国营国家知识产权局专利局专利审查协作北京中心北京100160

磁流变弹性体材料是在磁流变液的基础上发展而来,本文研究了与磁流变弹性体材料相关的专利技术发展态势。利用Incopat全球专利数据库,从全球和我国磁流变弹性体专利申请趋势、各国专利申请量对比、磁流变弹性体重要申请人及其专利申请... 详细信息

磁流变弹性体材料是在磁流变液的基础上发展而来,本文研究了与磁流变弹性体材料相关的专利技术发展态势。利用Incopat全球专利数据库,从全球和我国磁流变弹性体专利申请趋势、各国专利申请量对比、磁流变弹性体重要申请人及其专利申请量、全球和我国技术领域分布情况、国内外部分重要申请人的相关专利分析几个方面进行了检索分析,为我国磁流变弹性体材料研究提供信息和参考。

关键词：磁流变弹性体磁流变专利分析阻尼

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芯片超精密抛光用CMP抛光垫研究进展

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广东化工 2023年第16期50卷 62-64,57页

作者：周国营周文国家知识产权局专利局专利审查协作北京中心北京100160

化学机械抛光(CMP)技术是集成电路制造过程中实现晶圆表面平坦化的关键工艺,抛光垫是CMP工艺的关键原材料和耗材。本文从抛光垫的分类、基体材料、结构、作用、自修复及再生、制备工艺等方面进行了相关的综述。对目前抛光垫的研究中存... 详细信息

化学机械抛光(CMP)技术是集成电路制造过程中实现晶圆表面平坦化的关键工艺,抛光垫是CMP工艺的关键原材料和耗材。本文从抛光垫的分类、基体材料、结构、作用、自修复及再生、制备工艺等方面进行了相关的综述。对目前抛光垫的研究中存在的问题及发展前景进行了展望。

关键词：化学机械抛光抛光垫

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