Deletion of the waaf gene affects O antigen synthesis and pathogenicity in Vibrio parahaemolyticus from shellfish

作     者：Feng Zhao Guoying Ding Qilong Wang Huihui Du Guosheng Xiao Deqing Zhou Feng Zhao;Guoying Ding;Qilong Wang;Huihui Du;Guosheng Xiao;Deqing Zhou

作者机构：College of biology and food engineeringChongqing Three Gorges UniversityWanzhou 404100China Yellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesLaboratory for Marine Drugs and Bioproducts of Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266071China 

出 版 物：《Food Science and Human Wellness》 (食品科学与人类健康（英文）)

年 卷 期：2022年第11卷第2期

页      面：418-426页

核心收录：

学科分类：0832[工学-食品科学与工程（可授工学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 08[工学] 0822[工学-轻工技术与工程] 082203[工学-发酵工程] 

基　　金：supported by grants from the Natural Science Foundation of Chongqing(cstc2020jcyj-msxm X0685) the Science and Technology Research Program of Chongqing Municipal Education Commission(KJQN202001231) the National Natural Science Foundation of China(NSFC)(No.31201372) Science and Technology Project of Wanzhou in 2020。 

主　　题：Vibrio parahaemolyticus waaf gene LPS O antigen Pathogenicity 

摘      要：Vibrio parahaemolyticus is the main cause of foodborne gastroenteritis,which is widely distributed in shellfish and other seafood.Most V.parahaemolyticus are nonpathogenic,and only a few types,such as serotype O3:K6,are pathogenic,which is also the most prevalent strain in Asia.However,the relationship between this serotype and pathogenicity has yet to be established.The waaf gene is located in the O antigen synthesis gene cluster.Thus,we constructed a waaf gene deletion mutant(i.e.,Δwaaf)of wild-type(WT)which isolated from shellfish serotype O3:K6 via chitin-mediated transformation technology.We then constructed theΔwaaf complementary strain(i.e.,C-△waaf)via the Escherichia coli S17λpir strain by conjugation.The basic physiological characteristics,adhesion to Caco2 cells,and pathogenicity of the WT,△waaf,and C-△waaf strains were compared.Growth curves showed no remarkable differences between the WT andΔwaaf strains.However,theΔwaaf strain non-reactive to O3 antisera and other 12 O-group antisera of V.parahaemolyticus.Moreover,the number of flgella and extracellular polysaccharides decreased,the adhesion decreased,and the pathogenicity weakened.These characteristics of the C-△waaf strain were similar to those of the WT strain These results indicated that the waaf gene is vital to the serotype in V.parahaemolyticus,and changes in O antigen could affect the pathogenicity of this bacterium.This study will be helpful to understand the pathogenic mechanism of V.parahaemolyticus.