Background:After alleged stop of transmission of schistosomiasis and further down the line in post elimination settings,sensitive tools are required to monitor infection status to prevent potential *** Rahala,where tr...
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Background:After alleged stop of transmission of schistosomiasis and further down the line in post elimination settings,sensitive tools are required to monitor infection status to prevent potential *** Rahala,where transmission cycle of Schistosoma haematobium is interrupted since 2004 but where 30%of snails are still infected by ***,potential human *** infection can’t be *** methods based on egg-counts do not provide the required sensitivity,antibody or antigen assays are envisaged as the most appropriate tools for this type of ***:In this pilot study,the performances of three assays were compared:two commercially available antibody tests(ELISA and haemagglutination format)indicating exposure,and an antigen test(lateral flow strip format)demonstrating active *** 37 recruited study participants resided in Rahala(Akka,province Tata,Morocco).Participants had been diagnosed and cured from schistosomiasis in the period between 1983 and *** 2015 these asymptomatic participants provided fresh clinical samples(blood and urine)for analysis with the aforementioned diagnostics ***:No eggs were identified in the urine of the 37 *** haemagglutination test indicated 6 antibody positives whereas the ELISA indicated 28 antibody positives,one indecisive and one false *** and haemagglutination results matched for 18 individuals,amongst which 5 out of 6 haemagglutination *** the antigen test(performed on paired serum and urine samples),serum from two participants(cured 21 and 32 years ago)indicated the presence of low levels of the highly specific Schistosoma circulating anodic antigen(CAA),demonstrating low worm level infections(less than 5 pg/ml corresponding to probably single worm pair).One tested also CAA positive with *** indicated the presence of human anti-Schistosoma antibodies in these two CAA positive cases,haemagglutination results were ***:To pre
Background:Given the restricted distribution of Schistosoma mekongi in one province in Lao People’s Democratic Republic(Lao PDR)and two provinces in Cambodia,together with progress of the national control programmes ...
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Background:Given the restricted distribution of Schistosoma mekongi in one province in Lao People’s Democratic Republic(Lao PDR)and two provinces in Cambodia,together with progress of the national control programmes aimed at reducing morbidity and infection prevalence,the elimination of schistosomiasis mekongi seems ***,sensitive diagnostic tools will be required to determine whether elimination has been *** compared several standard and novel diagnostic tools in ***-endemic ***:The prevalence and infection intensity of *** were evaluated in 377 study participants from four villages in the endemic areas in Lao PDR and Cambodia using Kato-Katz stool examination,antibody detection based on an enzyme-linked immunosorbent assay(ELISA)and schistosome circulating antigen detection by lateral-flow *** highly sensitive test systems for the detection of cathodic and anodic circulating antigens(CCA,CAA)in urine and serum were ***:Stool microscopy revealed an overall prevalence of *** of 6.4%(one case in Cambodia and 23 cases in Lao PDR),while that of Opisthorchis viverrini,hookworm,Trichuris trichiura,Ascaris lumbricoides and Taenia *** 50.4%,28.1%,3.5%,0.3%and 1.9%,*** the urine samples,the tests for CCA and CAA detected *** infections in 21.0%and 38.7%of the study participants,*** the serum samples,the CAA assay revealed a prevalence of 32.4%,while a combination of the CAA assay in serum and in urine revealed a prevalence of 43.2%.There was a difference between the two study locations with a higher prevalence reached in the samples from Lao ***:The CCA,CAA and ELISA results showed substantially higher prevalence estimates for *** compared to Kato-Katz thick *** schistosomiasis mekongi in Lao PDR and Cambodia might thus have been considerably underestimated ***,sustained control efforts are still needed to break transmission of ***
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