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Identification of an avirulence gene,avrxa5,from the rice pathogen Xanthomonas oryzae ***

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Science China(Life Sciences) 2010年第12期53卷 1440-1449页

作者： ZOU HuaSong ZHAO WenXiang ZHANG XiFu HAN YangChun ZOU LiFang chen gongyou School of Agriculture and Biology Shanghai Jiaotong UniversityShanghai 200240China College of Plant Protection Nanjing Agricultural University/Laboratory for Monitoring and Management for Plant Pests and DiseasesChinese Ministry of AgricultureNanjing 210095China Department of Agronomy Henan Vocation-Technical Teachers CollegeXinxiang 453003China

Xanthomonas oryzae ***,the causal agent of bacterial blight in rice,interacts with rice plants in a gene-for-gene *** specificity of the interaction is dictated by avirulence(avr) genes in the pathogen and resistance(R) genes in the *** date,no avr genes that correspond to recessive R genes have been *** isolated an avrBs3/pthA family gene,avrxa5,from our previously isolated clone p58,which was originally from strain *** avrxa5 gene converted the PXO99A strain from compatible to incompatible in rice cultivars containing the recessive xa5 gene,but not in those containing the dominant Xa5 *** indicated that avrxa5,which is highly similar to members of the avrBs3/pthA family,encodes a protein of 1238 amino acid residues with a conserved carboxy-terminal region containing three nuclear localization signals and a transcription activation *** has 19.5 34-amino-acid direct repeats,but the 13th amino acid is missing in the fifth and ninth repetitive *** swapping of the repetitive regions between avrxa5 and avrXa7 changed the avirulence specificity of the genes in xa5 and Xa7 rice lines,*** indicates that avrxa5 is distinct from previously characterized avrBs3/pthA *** specificity of avrxa5 toward recessive xa5 in rice could help us better understand the molecular mechanisms of plant-pathogen specific interactions.

关键词： rice bacterial blight Xanthomonas oryzae pv.oryzae avrxa5 avirulence recessive resistance

Molecular studies of the Medicago truncatula MtAnn3 gene involved in root hair deformation

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Chinese Science Bulletin 2012年第15期57卷 1803-1809页

作者： GONG ZiYing SONG Xue chen gongyou ZHU JiaBi YU GuanQiao ZOU HuaSong National Laboratory of Plant Molecular Genetics Shanghai Institute of Plant Physiology and EcologyShanghai Institutes for Biological ScienceChinese Academy of SciencesShanghai 200032China School of Agriculture and Biology Shanghai Jiao Tong UniversityShanghai 200240China

The full-length annexin gene,MtAnn3,of Medicago truncatula was cloned by 5' *** with typical annexins,which contain a head domain and four homologous repeats in the conserved core domain,the MtAnn3 protein has only one repeat in the core ***3 can bind cell membranes when transiently expressed in onion epidermal *** rhizogenes-mediated transformation of MtAnn3 into Medicago roots revealed that overexpression of the gene can change the polarity of root hair growth in Ca2+-free *** plant hormone cytokinin was able to upregulate the expression of *** MtAnn3 transcripts were detected in young nodules,expression was not nodule-specific,and could be detected at high levels in the roots,stems and leaves as well.

关键词：蛋白基因苜蓿根分子生物学变形根毛膜联蛋白农杆菌介导细胞分裂素

The Xanthomonas typeⅢeffector Xop AP prevents stomatal closure by interfering with vacuolar acidification

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Journal of Integrative Plant Biology 2022年第10期64卷 1994-2008页

作者： Longyu Liu Ying Li Zhengyin Xu Huan chen Jingyi Zhang Brittany Manion Fengquan Liu Lifang Zou Zheng Qing Fu gongyou chen State Key Laboratory of Microbial Metabolism/Shanghai Collaborative Innovation Center of Agri‐Seeds School of Agriculture and BiologyShanghai Jiao Tong UniversityShanghai 200240China Department of Biological Sciences University of South CarolinaColumbia 29208USA Institute of Plant Protection Jiangsu Academy of Agricultural SciencesNanjing 210014China

Plant stomata close rapidly in response to a rise in the plant hormone abscisic acid(ABA)or salicylic acid(SA)and after recognition of pathogenassociated molecular patterns(PAMPs).Stomatal closure is the result of vacuolar convolution,ion efflux,and changes in turgor pressure in guard *** bacteria secrete typeⅢeffectors(T3Es)that interfere with plant defense mechanisms,causing severe plant disease ***,we show that the virulence and infection of Xanthomonas oryzae ***(Xoc),which is the causal agent of rice bacterial leaf streak disease,drastically increased in transgenic rice(Oryza sativa L.)plants overexpressing the Xoc T3E gene Xop AP,which encodes a protein annotated as a *** discovered that Xop AP binds to phosphatidylinositol 3,5-bisphosphate(Ptd Ins(3,5)P_(2)),a memb rane phospholipid that functions in p H control in lysosomes,membrane dynamics,and protein *** AP inhibited the acidification of vacuoles by competing with vacuolar H^(+)-pyrophosphatase(V-PPase)for binding to Ptd Ins(3,5)P_(2),leading to stomatal *** rice overexpressing Xop AP also showed inhibition of stomatal closure when challenged by Xoc infection and treatment with the PAMP ***,Xop AP suppressed flg22-induced gene expression,reactive oxygen species burst and callose deposition in host plants,demonstrating that Xop AP subverts PAMP-triggered immunity during Xoc *** together,these findings demonstrate that Xop AP overcomes stomatal immunity in plants by binding to lipids.

关键词： Ptd Ins(3 5)P2 stomatal closure typeⅢeffector vacuole acidification Xanthomonas Xop AP

Suppression of host plant defense by bacterial small RNAs packaged in outer membrane vesicles

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Plant Communications 2024年第4期5卷 280-282页

作者： Yan Wu Sai Wang Peihong Wang Wenhan Nie Iftikhar Ahmad Panagiotis F.Sarris gongyou chen Bo Zhu Shanghai Yangtze River Delta Eco-Environmental Change and Management Observation and Research Station Shanghai Cooperative Innovation Center for Modern Seed Industry School of Agriculture and Biology Shanghai Jiao Tong University Shanghai China Department of Environmental Sciences COMSATS University Islamabad Vehari-Campus Vehari 61100 Pakistan Department of Biology University of Crete 714 09 Heraklion Crete Greece Shanghai Jiao Tong University Chongqing Research Institute Shanghai China

(Plant Communications 5,100817;April 82024)After a careful,in-depth examination,the authors found some additional errors in the published *** revision,the authors created a mutant △Xosr001(in which the full-length Xosr001 was deleted)and a mutant △Xosr001’(in which the predicted binding motif of Xosr001 was deleted)as shown in Figure S4 of the original manuscript.

关键词： deleted Figure outer

Xa1 Allelic R Genes Activate Rice Blight Resistance Suppressed by Interfering TAL Effectors

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Plant Communications 2020年第4期1卷 54-62页

作者： Chonghui Ji Zhiyuan Ji Bo Liu He cheng Hua Liu Sanzhen Liu Bing Yang gongyou chen Division of Plant Sciences C.Bond Life Science CenterUniversity of MissouriColumbiaMO 65211USA School of Agriculture and Biology/State Key Laboratory of Microbial Metabolism Shanghai Jiao Tong UniversityShanghai 200240P.R.China National Key Facility for Crop Gene Resources and Genetic Improvement(NFCRI) Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081P.R.China Department of Plant Pathology Kansas State UniversityManhattanKS 66506USA Donald Danforth Plant Science Center St.LouisMO 63132USA

Xanthomonas oryzae pathovar oryzae(Xoo)uses transcription activator-like effectors(TALEs)to cause bacterial blight(BB)in *** turn,rice has evolved several mechanisms to resist BB by targeting *** mechanism involves the nucleotide-binding leucine-rich repeat(NLR)resistance gene Xa1 and ***,Xoo has evolved TALE variants,C-terminally truncated versions(interfering TALEs or iTALEs),to overcome Xa1 ***,it remains unknown to what extent the two co-adaptive mechanisms mediate Xoo–rice *** this study,we cloned and characterized five additional Xa1 allelic R genes,Xa2,Xa31(t),Xa14,CGS-Xo111,and Xa45(t)from a collection of rice *** analysis revealed that Xa2 and Xa31(t)from different rice cultivars are *** genes and their predicted proteins were found to be highly conserved,forming a group of Xa1 *** XA1 alleles could be distinguished by the number of C-terminal tandemrepeats consisting of 93 amino acid residues and ranged from four in XA14 to seven in XA45(t).Xa1 allelic genes were identified in the 3000 rice genomes *** the other hand,iTALEs could suppress the resistance mediated by Xa1 allelic R genes,and iTALE genes were prevalent(95%)in Asian,but not in African Xoo *** findings demonstrate the prominence of a defense mechanism in which rice depends on Xa1 alleles and a counteracting mechanismin which Xoo relies on iTALEs for BB.

关键词： Xa2 Xa14 Xa45(t) TAL effector iTAL effector bacterial blight

A Xanthomonas transcription activator-like effector is trapped in nonhost plants for immunity

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Plant Communications 2022年第1期3卷 71-84页

作者： Fazal Haq Xiameng Xu Wenxiu Ma Syed Mashab Ali Shah Linlin Liu Bo Zhu Lifang Zou gongyou chen School of Agriculture and Biology/State Key Laboratory of Microbial Metabolism Shanghai Jiao Tong UniversityShanghaiChina Key Laboratory of Urban Agriculture of the Ministry of Agriculture Shanghai200240China

Xanthomonas oryzae ***(Xoo),the causal agent of bacterial leaf blight in rice,delivers transcription activator-like effector(TALE)proteins into host cells to activate susceptibility or resistance(R)genes that promote disease or immunity,*** plants serve as potential reservoirs of R genes;consequently,nonhost R genes may trap TALEs to trigger an immune *** this study,we screened 17 Xoo TALEs for their ability to induce a hypersensitive response(HR)in the nonhost plant Nicotiana benthamiana(Nb);only AvrXa10 elicited an HR when transiently expressed in *** HR generated by AvrXa10 required both the central repeat region and the activation domain,suggesting a specific interaction between AvrXa10 and a potential R-like gene in nonhost *** blue staining and ion leakage measurements confirmed that the AvrXa10-triggered HR was a form of cell death,and the transient expression of AvrXa10 in Nb induced immune *** targeted by AvrXa10 in the Nb genome were identified by transcriptome profiling and prediction of effector binding *** several approaches(in vivo reporter assays,electrophoretic mobility-shift assays,targeted designer TALEs,and on-spot gene silencing),we confirmed that AvrXa10 targets NbZnFP1,a C2H2-type zinc finger protein that resides in the *** analysis indicated that overexpression of NbZnFP1 and its rice orthologs triggered cell death in rice *** NbZnFP1 ortholog was also identified in tomato and was specifically activated by *** results demonstrate that NbZnFP1 is a nonhost R gene that traps AvrXa10 to promote plant immunity in Nb.

关键词： Xanthomonas oryzae pv.oryzae AvrXa10 hypersensitive response nonhost plant zinc finger protein

A Potential Disease Susceptibility Gene CsLOB of Citrus Is Targeted by a Major Virulence Effector PthA of Xanthomonas citri subsp, citri

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Molecular Plant 2014年第5期7卷 912-915页

作者： Zheng Li Lifang Zou Gang Ye Li Xiong Zhiyuan Ji Muhammad Zakria Ni Hong Guoping Wang gongyou chen School of Agriculture and Biology Shanghai Jiao Tong University Shanghai 200240 China State Key Laboratory of Microbial Metabolism School of Life Science &Biotechnology Shanghai Jiao Tong University Shanghai 200240 China National Key Laboratory of Agromicrobiology Huazhong Agricultural University Wuhan Hubei 430070 China

Dear Editor, Citrus bacterial canker （CBC）, one of the most destructive diseases of citrus, has a severe economic impact on citrus industries in tropical and subtropical countries （Das, 2003）. It has been widely known that Xanthomonas citri subsp, citri （Xcc） causes the CBC by transferring the type-Ill effectors （T3SEs） to the interior of the plant cell through type-Ill secretion system （T3S） to modulate transcription of host plants to trigger CBC disease （Schaad et al., 2006）. Pathotype A,

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Tal6b/AvrXa27A,a hidden TALE targeting the susceptibility gene OsSWEET11a and the resistance gene Xa27 in rice

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Plant Communications 2024年第2期5卷 122-134页

作者： Zhengyin Xu Xiameng Xu Ying Li Linlin Liu Qi Wang Yijie Wang Yong Wang Jiali Yan Guanyun cheng Lifang Zou Bo Zhu gongyou chen Shanghai Collaborative Innovation Center of Agri-Seeds School of Agriculture and BiologyShanghai Jiao Tong UniversityShanghai 200240China State Key Laboratory of Microbial Metabolism Shanghai Jiao Tong UniversityShanghai 200240China

Xanthomonas oryzae ***(Xoo)secretes transcription activator-like effectors(TALEs)to activate rice susceptibility(S)genes,causing bacterial blight(BB),as well as resistance(R)genes,leading to de-fense against *** activation follows a gene-for-gene paradigm that results in an arms race between the TALE of the pathogen and effector-binding elements(EBEs)in the promoters of host *** this study,we characterized a novel TALE,designated Tal6b/AvrXa27A,that activates the rice S gene OsSWEET11a and the rice R gene ***6b/AvrXa27A is a member of the AvrXa27/TalAO class and contains 16 repeat variable diresidues(RVDs);one RVD is altered and one is deleted in Tal6b/AvrXa27A compared with AvrXa27,a known avirulence(avr)effector of ***6b/AvrXa27A can transcriptionally activate the expression of Xa27 and OsSWEET11a via EBEs in their corresponding promoters,leading to effector-triggered immunity and susceptibility,*** 16 RVDs in Tal6b/AvrXa27A have no obvious similarity to the 24 RVDs in the effector PthXo1,but EBETal6b and EBEPthXo1 are overlapped in the OsSWEET11a ***6b/AvrXa27A is prevalent among Asian Xoo isolates,but PthXo1 has only been reported in the Philippine strain *** editing of EBETal6b in the OsSWEET11a pro-moter further confirmed the requirement for OsSWEET11a expression in Tal6b/AvrXa27A-dependent susceptibility to ***,Tal6b/AvrXa27A resulted in higher transcription of Xa27 than of OsSWEET11a,which led to a strong,rapid resistance response that blocked disease *** suggest that Tal6b/AvrXa27A has a dual function:triggering resistance by activating Xa27 gene expression as an avirulence factor and inducing transcription of the S gene OsSWEET11a,resulting in ***,Tal6b/AvrXa27A,but not AvrXa27,can bind to the promoter of *** underlying recognition mechanism for this binding remains unclear but appears to deviate from the currently accepted TALE code.

关键词： Xanthomonas oryzae pv.oryzae TALE AvrXa27 tal6b Xa27 OsSWEET11a

Engineering Broad-Spectrum Bacterial Blight Resistance by Simultaneously Disrupting Variable TALE-Binding Elements of Multiple Susceptibility Genes in Rice

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Molecular Plant 2019年第11期12卷 1434-1446页

作者： Zhengyin Xu Xiameng Xu Qiang Gong Ziyang Li Ying Li Sai Wang Yangyang Yang Wenxiu Ma Longyu Liu Bo Zhu Lifang Zou gongyou chen School of Agriculture and Biology/State Key Laboratory of Microbial Metabolism Shanghai Jiao Tong UniversityShanghai 200240China

Xanthomonas oryzae ***(Xoo),the causal agent of bacterial blight of rice,employs the transcription activator-like effectors(TALEs)to induce the expression of the OsSWEET family of putative sugar transporter genes,which function in conferring disease susceptibility(S)in rice *** engineer broadspectrum bacterial blight resistance,we used CRISPR/Cas9-mediated gene editing to disrupt the TALEbinding elements(EBEs)of two S genes,OsSWEETH and OsSWEET14,in rice ***,which harbors the recessive resistance allele of Xa25/*** engineered rice line MS14K exhibited broadspectrum resistance to most Xoo strains with a few exceptions,suggesting that the compatible strains may contain new *** identified two PthXo2-like TALEs,Tal5LN18 and Tal7PX061,as major virulence factors in the compatible Xoo strains LN18 and PX061,respectively,and found that Xoo encodes at least five types of PthXo2-like *** that PthXo2/PthXo2.1 target OsSlVEETf3 for transcriptional activation,the genomes of 3000 rice varieties were analyzed for EBE variationsin the OsSWEET13 promoter,and 10Xa25-like haplotypes were *** found that Tal5LN18 and Tal7PX〇6i bind slightly different EBE sequences in the OsSWEET13 promoter to activate its ***/Cas9 technology was then used to generate InDels in the EBE of the OsSWEET13 promoter in MS14K to creat a new germplasm with three edited OsSWEET EBEs and broad-spectrum resistance against all Xoo strains ***,our findings illustrate how to disarm TALE-S co-evolved loci to generate broad-spectrum resistance through the loss of effector-triggered susceptibility in plants.

关键词： Xanthomonas oryzae pv.oryzae TALE susceptible gene ETS RLS