The Xanthomonas typeⅢeffector Xop AP prevents stomatal closure by interfering with vacuolar acidification

作     者：Longyu Liu Ying Li Zhengyin Xu Huan Chen Jingyi Zhang Brittany Manion Fengquan Liu Lifang Zou Zheng Qing Fu Gongyou Chen Longyu Liu;Ying Li;Zhengyin Xu;Huan Chen;Jingyi Zhang;Brittany Manion;Fengquan Liu;Lifang Zou;Zheng Qing Fu;Gongyou Chen

作者机构：State Key Laboratory of Microbial Metabolism/Shanghai Collaborative Innovation Center of Agri‐SeedsSchool of Agriculture and BiologyShanghai Jiao Tong UniversityShanghai 200240China Department of Biological SciencesUniversity of South CarolinaColumbia 29208USA Institute of Plant ProtectionJiangsu Academy of Agricultural SciencesNanjing 210014China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2022年第64卷第10期

页      面：1994-2008页

核心收录：

学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 

基　　金：supported by the Shanghai Agriculture Applied Technology Development Program,China(2020-02-08-00-08-F01462) the National Natural Science Foundation of China(31830072,32102147) 

主　　题：Ptd Ins(3 5)P2 stomatal closure typeⅢeffector vacuole acidification Xanthomonas Xop AP 

摘      要：Plant stomata close rapidly in response to a rise in the plant hormone abscisic acid(ABA)or salicylic acid(SA)and after recognition of pathogenassociated molecular patterns(PAMPs).Stomatal closure is the result of vacuolar convolution,ion efflux,and changes in turgor pressure in guard *** bacteria secrete typeⅢeffectors(T3Es)that interfere with plant defense mechanisms,causing severe plant disease ***,we show that the virulence and infection of Xanthomonas oryzae ***(Xoc),which is the causal agent of rice bacterial leaf streak disease,drastically increased in transgenic rice(Oryza sativa L.)plants overexpressing the Xoc T3E gene Xop AP,which encodes a protein annotated as a *** discovered that Xop AP binds to phosphatidylinositol 3,5-bisphosphate(Ptd Ins(3,5)P_(2)),a memb rane phospholipid that functions in p H control in lysosomes,membrane dynamics,and protein *** AP inhibited the acidification of vacuoles by competing with vacuolar H^(+)-pyrophosphatase(V-PPase)for binding to Ptd Ins(3,5)P_(2),leading to stomatal *** rice overexpressing Xop AP also showed inhibition of stomatal closure when challenged by Xoc infection and treatment with the PAMP ***,Xop AP suppressed flg22-induced gene expression,reactive oxygen species burst and callose deposition in host plants,demonstrating that Xop AP subverts PAMP-triggered immunity during Xoc *** together,these findings demonstrate that Xop AP overcomes stomatal immunity in plants by binding to lipids.