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Fusion of a rice endogenous N-methylpurine DNA glycosylase to a plant adenine base transition editor ABE8e enables A-toK base editing in rice plants

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aBIOTECH 2024年第2期5卷 127-139页

作者： Yucai li Shaoya li Chenfei li Chen Zhang Lei yan Jingying li Yubing He yan Guo Lanqin Xia Institute of Crop Sciences(ICS) Chinese Academy of Agricultural Sciences(CAAS)Beijing 100081China State Key Laboratory of Plant Physiology and Biochemistry College of Biological SciencesChina Agricultural UniversityBeijing 100193China Hainan Yazhou Bay Seed Laboratory/National Nanfan Research Institute(Sanya) CAASSanya 572024China

Engineering of a new type of plant base editor for simultaneous adenine transition and transversion within the editing window will greatly expand the scope and potential of base editing in directed evolution and crop ***,we isolated a rice endogenous hypoxanthine excision protein,N-methylpurine DNA glycosylase(OsMPG),and engineered two plant A-to-K(K=G or T)base editors,rAKBE01 and rAKBE02,for simultaneous adenine transition and transversion base editing in rice by fusing OsMPG or its mutant mOsMPG to a plant adenine transition base editor,*** further coupled either OsMPG or mOsMPG with a transactivation factor VP64 to generate rAKBE03 and rAKBE04,*** these four rAKBEs,at five endogenous loci in rice protoplasts,indicated that rAKBE03 and rAKBE04 enabled higher levels of A-to-G base transitions when compared to ABE8e and ***,whereas rAKBE01 only enabled A-to-C/T editing at one endogenous locus,in comparison with rAKBE02 and rAKBE03,rAKBE04 could significantly improve the A-to-C/T base transversion efficiencies by up to 6.57-and 1.75-fold in the rice protoplasts,***,although no stable lines with A-to-C transversion were induced by rAKBE01 and rAKBE04,rAKBE04 could enable simultaneous A-to-G and A-to-T transition and transversion base editing,at all the five target loci,with the efficiencies of A-to-G transition and A-to-T transversion editing ranging from 70.97 to 92.31%and 1.67 to 4.84%in rice stable lines,***,these rAKBEs enable different portfolios of editing products and,thus,now expands the potential of base editing in diverse application scenario for crop improvement.

关键词： Rice(Oryza sativa L) Rice N-methylpurine DNA glycosylase(OsMPG) A-to-K base editor(AKBE) Transactivation module VP64

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Enhancing Tea Leaf Disease Identification with lightweight MobileNetV2

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Computers, Materials & Continua 2024年第7期80卷 679-694页

作者： Zhilin li Yuxin li Chunyu yan Peng yan Xiutong li Mei Yu Tingchi Wen Benliang Xie College of Big Data and Information Engineering Guizhou UniversityGuiyang550025China Power Semiconductor Device Reliability Engineering Center of the Ministry of Education Guiyang550025China State Key Laboratory of Public Big Data Guizhou UniversityGuiyang550025China The Mushroom Research Centre Guizhou UniversityGuiyang550025China The Engineering Research Center of Southwest Bio-Pharmaceutical Resources Ministry of EducationGuizhou UniversityGuiyang550025China

Diseases in tea trees can result in significant losses in both the quality and quantity of tea *** monitoring can help to prevent the occurrence of large-scale diseases in tea ***,existingmethods face challenges such as a high number of parameters and low recognition accuracy,which hinders their application in tea plantation monitoring *** paper presents a lightweight I-MobileNetV2 model for identifying diseases in tea leaves,to address these *** proposed method first embeds a Coordinate Attention(CA)module into the originalMobileNetV2 network,enabling the model to locate disease regions ***,a Multi-branch Parallel Convolution(MPC)module is employed to extract disease features across multiple scales,improving themodel’s adaptability to different disease ***,the AutoML for Model Compression(AMC)is used to compress themodel and reduce computational *** results indicate that our proposed algorithm attains an average accuracy of 96.12%on our self-built tea leaf disease dataset,surpassing the original MobileNetV2 by 1.91%.Furthermore,the number of model parameters have been reduced by 40%,making itmore suitable for practical application in tea plantation environments.

关键词： Disease identification coordinate attention mechanism multi-scale feature extraction model pruning

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Impact Performance Research of Re-Entrant Octagonal Negative Poisson’s Ratio Honeycomb with Gradient Design

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Computer Modeling in Engineering & Sciences 2024年第9期140卷 3105-3119页

作者： Yiyuan li Yongjing li Shilin yan Pin Wen Department of Engineering Structure and Mechanics Wuhan University of TechnologyWuhan430070China Hubei Key Laboratory of Theory and Application of Advanced Materials Mechanics School of ScienceWuhan University of TechnologyWuhan430070China

Based on the traditional re-entrant honeycomb,a novel re-entrant octagon honeycomb(ROH)is *** deformation mode of the honeycomb under quasi-static compression is analyzed by numerical simulation,and the results are in good agreement with the experimental *** deformation modes,mechanical properties,and energy absorption characteristics of ROH along the impact and perpendicular directions gradient design are investigated under different *** results indicated that the deformation mode of ROH is affected by gradient design along the direction of impact and impact *** addition,gradient design along the direction of impact can increase the initial peak stress of ROH and accelerate its densification *** design perpendicular to the impact direction can enhance the energy absorption performance of ROH,especially for ROH,with wall thickness increasing from the inside *** to ROH with uniform wall thickness at the same relative density,ROH with a gradient design can increase the plateau stress by over *** the elevation of impact velocity,the plateau stress and specific energy absorption exhibit an upward trend,aligning with the dynamic performance pattern observed in conventional *** results can be used as a reference for the design and application of honeycomb and provide a new idea for developing more efficient and reliable energy-absorbing materials.

关键词： Re-entrant honeycomb gradient design finite element analysis additive manufacturing technologies

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A new integrated model of deformation resistance and its application in prediction of rolling force of a thick plate

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Journal of Iron and Steel Research International 2024年第4期31卷 882-893页

作者： Shun-hu Zhang yan li li-zhi Che Wen-hao Tian Shagang School of Iron and Steel Soochow UniversitySuzhou 215021JiangsuChina

The prediction accuracy of existing models of the rolling force of a thick plate is always very *** address this problem,a high-precision genetic algorithm-backpropagation network(GA-BP)model of deformation resistance was built,and its integration with the traditional fitted model was further ***,a novel rolling force model was obtained by embedding the integration model of deformation resistance in the original model of rolling *** to this research idea,the industrial data are normalized at *** this basis,the interactions among the process parameters were disclosed through the variance analysis,and then described by various virtual *** factors are set as part of input ***,the optimal structure of the GA-BP model of deformation resistance was determined and an integration model of deformation resistance was ***,a novel rolling force model is obtained by substituting the traditional fitted deformation resistance into the Sims model with the integration model of the deformation *** results proves that the introduction of virtual factors can increase the hit rate of±5%from 75.8%to 78%and can reduce the root mean square error from 4.72%to 4.48%.Besides,it is found that the mean relative error of the traditional fitted deformation resistance is 0.142,while that of the modified deformation resistance is only *** addition,the mean relative error in the original rolling force model is 0.145,while that of the present model is only 0.03.

关键词： Integration model-Deformation resistance Variance analysis Virtual factor Genetic algorithm-backpropagation network model

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Attributes of Specialized Households'Resilience and Its Impact on Rural Industrial Advancement:A Case Study of National Musical Instrument Production Specialized Households in Lankao County,Henan,China

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Chinese Geographical Science 2024年第3期34卷 383-400页

作者： WU Nalin WEI Yike FAN Sizhe li li SUN Yufan ZHANG yan SHU yan Key Laboratory of Geospatial Technology for the Middle and Lower Yellow River Regions(Henan University) Ministry of Educa-tion/The College of Geography and Environmental ScienceHenan UniversityKaifeng 475001China College of Urban and Envir-onmental Science Central China Normal UniversityWuhan 430079China Key Laboratory of Ecosystem Network Observation and Modelling Institute of Geographic Sciences and Natural Resources ResearchChinese Academy of SciencesBeijing 100101China

Specialized households serve as the primary units within specialized villages in China,and their capacity to withstand risks and external influences significantly shapes the future trajectory of specialized villages and the overall vitality of the rural *** this study,we established a measurement indicator system based on the definition of specialized households’resilience,elucidating the logical connection between specialized households’resilience and rural industrial development in *** musical instrument industry in Lankao County,Henan Province of China,was employed as a case;survey data,the entropy method,and an obstacle diagnosis model were used to examine how instrument production specialized households responded to the challenges posed by Corona Virus Disease 2019(COVID-19)and the tightening of national environmental protection policies,yielding the following key findings:1)there exists substantial variation in the comprehensive resilience levels among different specialized households;2)the ability to learn and adapt is the most significant contributor to the overall resilience level of specialized households;3)technological proficiency and access to skilled talent emerge as pivotal factors influencing specialized households’resilience;4)the positioning of specialized households within the industrial supply chain and the stability of their income have a direct bearing on their resilience *** influence of specialized households’resilience on industrial development primarily manifests in the following ways:stronger resilience correlates with increased stability in production and sales,fostering a more proactive approach to future ***,heightened exposure to the external macroeconomic environment can lead to a higher rate of export *** enhance the development resilience of entities like specialized households and family farms,and to invigorate rural economic development,escalating investments in rural science and technology

关键词： resilience specialized households rural industry Lankao County,China

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Comprehensive Exergoeconomic Analysis and Optimization of a Novel Zero-Carbon-Emission Multi-Generation System based on Carbon Dioxide Cycle

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Journal of Thermal Science 2024年第3期33卷 1065-1081页

作者： SUN yan li Hongwei WANG Di DU Changhe School of Energy and Power Engineering Northeast Electric Power UniversityJilin132012China School of Automation Engineering Northeast Electric Power UniversityJilin132012China Xi’an Institute of Electromechanical Information Technology Xi’an710065China

This paper aims to conduct a comprehensive exergoeconomic analysis of a novel zero-carbon-emission multi-generation system and propose a fast optimization method combined with machine *** detailed exergoeconomic analysis of a novel combined power,freshwater and cooling multi-generation system is performed in this *** exergoeconomic analysis model is established by exergy flow theory.A comprehensive exergy,exergoeconomic and environmental analysis is carried *** critical decision variables are researched to bring out effects on the multi-generation system exergoeconomic performance.A novel fast optimization method combining genetic algorithm and Bagging neural network is *** advanced nature comparison is made between the proposed system and four similar *** display that increasing the turbine inlet temperature can improve exergy efficiency and decrease the total product unit *** multi-generation system exergy destruction directly determines exergy efficiency and total exergy destruction cost *** total product unit cost in the cost optimal design case is reduced by 7.7%and 25%,respectively,compared with exergy efficiency optimal design case and basic design *** with four similar cases,the proposed multi-generation system has great advantages in thermodynamic performance and exergoeconomic *** paper can provide research methods and ideas for performance analysis and fast optimization of multi-generation system.

关键词： waste heat recovery exergoeconomic analysis supercritical carbon dioxide optimization machine learning

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Omics for deciphering oral microecology

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International Journal of Oral Science 2024年第2期16卷 197-207页

作者： Yongwang lin Xiaoyue liang Zhengyi li Tao gong Biao Ren Yuqing li Xian Peng State Key Laboratory of Oral Diseases&National Center for Stomatology&National Clinical Research Center for Oral Diseases West China Hospital of StomatologySichuan UniversityChengduChina

The human oral microbiome harbors one of the most diverse microbial communities in the human body,playing critical roles in oral and systemic *** technological innovations are propelling the characterization and manipulation of oral ***-throughput sequencing enables comprehensive taxonomic and functional profiling of oral *** long-read platforms improve genome assembly from complex ***-cell genomics provides insights into uncultured *** imaging modalities including fluorescence,mass spectrometry,and Raman spectroscopy have enabled the visualization of the spatial organization and interactions of oral microbes with increasing *** techniques link phylogenetic identity with *** spectrometry imaging reveals metabolic niches and activities while Raman spectroscopy generates rapid biomolecular fingerprints for *** facilitates the isolation and cultivation of novel fastidious oral taxa using high-throughput *** integration of these technologies holds the promise of transforming our understanding of oral microbiome assembly,gene expression,metabolites,microenvironments,virulence mechanisms,and microbe-host interfaces in the context of health and ***,significant knowledge gaps persist regarding community origins,developmental trajectories,homeostasis versus dysbiosis triggers,functional biomarkers,and strategies to deliberately reshape the oral microbiome for therapeutic *** convergence of sequencing,imaging,cultureomics,synthetic systems,and biomimetic models will provide unprecedented insights into the oral microbiome and offer opportunities to predict,prevent,diagnose,and treat associated oral diseases.

关键词： insight holds oral

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A Modified Iterative Learning Control Approach for the Active Suppression of Rotor Vibration Induced by Coupled Unbalance and Misalignment

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Chinese Journal of Mechanical Engineering 2024年第1期37卷 242-253页

作者： Yifan Bao Jianfei Yao Fabrizio Scarpa yan li College of Mechanical and Electrical Engineering Beijing University of Chemical TechnologyBeijing 100029China State Key Laboratory of High-end Compressor and System Technology Beijing 100029China Beijing Key Laboratory of High-end Mechanical Equipment Health Monitoring and Self-Recovery Beijing University of Chemical TechnologyBeijing 100029China Key Lab of Engine Health Monitoring-Control and Networking of Ministry of Education Beijing University of Chemical TechnologyBeijing 100029China Bristol Composites Institute(ACCIS) University of BristolBristol BS81TRUK Dynamics and Control Research Group(DCRG) University of BristolBristol BS81TRUK

This paper proposes a modified iterative learning control(MILC)periodical feedback-feedforward algorithm to reduce the vibration of a rotor caused by coupled unbalance and parallel *** control of the vibration of the rotor is provided by an active magnetic actuator(AMA).The iterative gain of the MILC algorithm here presented has a self-adjustment based on the magnitude of the *** filters are adopted to extract the synchronous(1×Ω)and twice rotational frequency(2×Ω)components of the rotor *** the notch frequency of the filter and the size of feedforward storage used during the experiment have a real-time adaptation to the rotational *** method proposed in this work can provide effective suppression of the vibration of the rotor in case of sudden changes or fluctuations of the rotor *** and experiments using the MILC algorithm proposed here are carried out and give evidence to the feasibility and robustness of the technique proposed.

关键词： Rotor vibration suppression Modified iterative learning control Unbalance Parallel misalignment Active magnetic actuator

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Robust Interval State Estimation for Distribution Systems Considering Pseudo-measurement Interval Prediction

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Journal of Modern Power Systems and Clean Energy 2024年第1期12卷 179-188页

作者： Xu Zhang Wei yan Meiqing Huo Hui li the School of Electrical Engineering Chongqing UniversityChongqing 400044China

Interval state estimation(ISE)can estimate state intervals of power systems according to confidence intervals of predicted pseudo-measurements,thereby analyzing the impact of uncertain pseudo-measurements on ***,predicted pseudo-measurements have prediction errors,and their confidence intervals do not necessarily contain the truth values,leading to estimation biases of the *** solve this problem,this paper proposes a pseudo-measurement interval prediction framework based on the Gaussian process regression(GPR)model,thereby improving the prediction accuracy of pseudo-measurement confidence ***,a weight assignment strategy for improving the robustness of weighted least squares(WLS)ISE is *** strategy quantifies the deviation between the pseudo-measurement intervals and their estimated intervals and assigns smaller weights to the pseudo-measurement intervals with larger deviations,thereby improving the estimation accuracy and robustness of the *** paper adopts the data from the supervisory control and data acquisition(SCADA)system of the New York Independent System Operator(NYISO).It verifies the advantages of the GPR method for pseudo-measurement interval prediction by comparing it with the quantile regression and neural network *** addition,this paper demonstrates the effectiveness of the proposed weight assignment strategy through the IEEE 14-bus ***,the differences in the estimation accuracy and the bad data identification between the robust interval state estimation and deterministic state estimation are discussed.

关键词： Interval state estimation interval analysis pseudo-measurement Gaussian process regression(GPR)

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Efficacy of a Nutritional Cream Intervention to Treat Depression in Rescuers:A Randomized Controlled Trial

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Biomedical and Environmental Sciences 2024年第7期37卷 754-761页

作者： Qiao Wang Hengyu Luan Chunyan li Rufang gong Qiongxuan li Jiayi Deng Xiaoyong Sai Department of Statistics and Epidemiology The Graduate School of the Chinese PLA General HospitalBeijing 100853China Comprehensive Internal Medicine Western Medical Branch of PLA General HospitalBeijing 100144China Department of Respiratory The First Medical Center of PLA General HospitalBeijing 100853China Department of Pharmacy Intravenous Admixture Services(PIVAS) The First Medical Center of PLA General HospitalBeijing 100853China

Objective To explore the effectiveness of a nutritional intervention in rescuers who screened positive for *** A randomized controlled trial design was *** June to August,2022,4,460 rescuers were screened using the Self-Rating Depression Scale(SDS),and 1,615 positive cases were ***-one volunteers were recruited and randomly divided into a nutritional intervention group and a control *** intervention group received health education and nutritional intervention(a compound paste therapy primarily composed of red roses and Seville orange flowers),while the control group received psychological *** scores were assessed before and after the *** There was a statistically significant decline in SDS scores in the nutritional intervention group after the intervention(P<0.05).Furthermore,the SDS scores of the intervention group were significantly lower than those of the control group,both before and after the intervention(P<0.05).No severe adverse reactions were observed during safety *** The nutritional intervention effectively reduced the depression scores in *** nutritional intervention is recommended for rescuers who initially screen positive for depression.

关键词： Nutritional intervention Rescuers Depression Randomized controlled trial

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