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Development and evaluation of specific PCR primers targeting the ribosomal DNA-internal transcribed spacer(ITS)region of peritrich ciliates in environmental samples

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Journal of Oceanology and Limnology 2018年第3期36卷 818-826页

作者： SU Lei zhANG Qianqian gong Jun Laboratory of Microbial Ecology and Matter Cycles Yantai Institute of Coastal Zone ResearchChinese Academy of SciencesYantai 264003China University of Chinese Academy of Sciences Beijing 100049China

Peritrich ciliates are highly diverse and can be important bacterial grazers in aquatic ecosystems. Morphological identifi cations of peritrich species and assemblages in the environment are time-consuming and expertise-demanding. In this study, two peritrich-specifi c PCR primers were newly designed to amplify a fragment including the internal transcribed spacer(ITS) region of ribosomal rDNA from environmental samples. The primers showed high specifi city in silico, and in tests with peritrich isolates and environmental DNA. Application of these primers in clone library construction and sequencing yielded exclusively sequences of peritrichs for water and sediment samples. We also found the ITS1, ITS2, ITS, D1 region of 28 S rDNA, and ITS+D1 region co-varied with, and generally more variable than, the V9 region of 18 S rDNA in peritrichs. The newly designed specifi c primers thus provide additional tools to study the molecular diversity, community composition, and phylogeography of these ecologically important protists in dif ferent systems.

关键词： Ciliophora Peritrichia clone library internal transcribed spacer(ITS) rDNA specific PCR primers

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通过超声扫描诊断单绒毛膜双胎的并发症

中国产前诊断杂志（电子版） 2010年第4期2卷 52-53页

作者： gong LJ,Wei Y,Liu zh,zhao YY 北京大学第三医院妇产科

目的 :评价超声在诊断双胎妊娠方面的作用。方法 :回顾性分析2007年1月～2010年6月期间132例单绒毛膜双胎。结果 :第一组为123例(单绒毛膜双羊膜囊双胎组),包括18例双胎输血综合征(14.6%)。

关键词：单绒毛膜双胎并发症双胎输血综合征双羊膜囊双胎双胎妊娠回顾性分析诊断超声扫描胎儿生长胎死亡

Evaluation of Reference Genes for Quantitative Real-Time PCR Analysis in Manila Clam Ruditapes philippinarum Under Hypoxic Stress

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Journal of Ocean University of China 2023年第4期22卷 1059-1067页

作者： JING Hao zhOU Liqing gong Miao TU Kang LIU zhihong WU Biao SUN Xiujun College of Fisheries and Life Science Shanghai Ocean UniversityShanghai 201306China State Key Laboratory of Mariculture Biobreeding and Sustainable Goods Yellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266071China Laboratory for Marine Fisheries Science and Food Production Processes Laoshan LaboratoryQingdao 266237China School of Marine Science and Fisheries Jiangsu Ocean UniversityLianyungang 222005China Putian Institute of Aquaculture Science of Fujian Province Putian 351100China

Quantitative real-time PCR(qRT-PCR)has been widely used for gene expression analysis,and selection of reference genes is a key point to obtain accurate *** find out optimal reference genes for qRT-PCR in Manila clam Ruditapes philippinarum in response to hypoxia,different tissues were used and compared to evaluate the stability of candidate reference genes under low oxygen stress(DO 0.5mgL^(−1) and DO 2.0mgL^(−1))and normal condition(DO 7.5mgL^(−1)).Seven candidate reference genes were selected to evaluate the stability of their expression *** reference genes were evaluated by Delta Ct,BestKeeper,NormFinder and geNorm,and then screened by RefFinder *** hypoxic stress of 0.5mgL^(−1),the most suitable reference gene for gill and hepatopancreas was RPL31,and the optimal reference genes for axe foot and adductor muscle were TUB and HIS,*** hypoxic stress of 2.0mgL^(−1),the most stable reference gene for gill and hepatopancreas was RPL31,and the optimal reference genes for axe foot and adductor muscle were RPS23 and EF1A,*** the normal condition,HIS and EF1A were identified as the optimal internal reference genes in gill and hepatopancreas respectively,and GFRP2 was the best internal reference gene for axe foot and adductor *** present findings will provide important basis for the selection of reference genes for qRT-PCR analysis of gene expression level in bivalves under hypoxic stress,which might be helpful for the analysis of other molluscs too.

关键词： clam reference gene hypoxia quantitative real-time PCR

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浅谈UPS电源及蓄电池选型配置维护保养

青海师范大学学报（自然科学版） 2014年第3期30卷 39-42页

作者：赵公义青海省广播电视局919台青海尖扎811299

中波广播发送设备已由低质量、高能耗、难维护的电子管发射机更新为高效率、高质量、高稳定的全固态数字式调制发射机,而供配电系统的安全可靠对于机房中心广播电视信号的安全播出显得尤为重要.电源及配电系统是机房系统的动力来源,必... 详细信息

中波广播发送设备已由低质量、高能耗、难维护的电子管发射机更新为高效率、高质量、高稳定的全固态数字式调制发射机,而供配电系统的安全可靠对于机房中心广播电视信号的安全播出显得尤为重要.电源及配电系统是机房系统的动力来源,必须有持续不间断的动力供电保障才能保证设备的安全可靠运行,确保安全播出,它的稳定、可靠是机房内各系统正常运行的重要的基础保证.本文将从动力保障"机房供电"方面谈谈安全播出.

关键词：双回路供电 UPS 电源免维护铅酸蓄电池

何首乌丸加味干预后衰老模型大鼠睾丸生精细胞p53基因表达的变化

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中国组织工程研究与临床康复 2007年第17期11卷 3379-3381,F0003页

作者：陈志宏龚秀云李健薛景凤承德医学院基础医学研究所河北省承德市067000 承德市中心医院耳鼻喉科河北省承德市067000

目的:观察中药何首乌丸加味对衰老模型大鼠睾丸生精细胞凋亡、血清睾酮含量和抗氧化能力的影响。方法:实验于2004-04/07在承德医学院基础医学研究所完成。①选用40只雄性SD大鼠,随机分为正常组、模型组、何首乌丸加味组和阴性对照组,每... 详细信息

目的:观察中药何首乌丸加味对衰老模型大鼠睾丸生精细胞凋亡、血清睾酮含量和抗氧化能力的影响。方法:实验于2004-04/07在承德医学院基础医学研究所完成。①选用40只雄性SD大鼠,随机分为正常组、模型组、何首乌丸加味组和阴性对照组,每组均为10只。模型组、何首乌丸加味组和阴性对照组均采用D-半乳糖200mg/(kg·d)连续腹腔注射40d制作亚急性衰老大鼠模型。模型组大鼠于造模成功后不再作任何处理;何首乌丸加味用药组大鼠于造模成功后用何首乌丸加味(由何首乌15g,怀牛膝15g,肉苁蓉10g,淫羊藿10g,丹参25g,茯苓15g组成,每副含生药90g,按传统方法加水煎成溶液)8.1g/kg灌胃给药,1次/d,共30d;阴性对照组大鼠于造模成功后每天灌胃同等剂量的生理盐水,时间同何首乌丸加味用药组。正常组大鼠自由摄食、饮水,未加任何干预措施。②所有大鼠经内眦取血2mL,采用酶联免疫吸附法检测各组大鼠血清睾酮含量、黄嘌呤氧化酶法检测血清超氧化物歧化酶的活性;大鼠处死后取睾丸组织、常规石蜡包埋,SP免疫组化法检测睾丸生精细胞p53基因的表达。结果:大鼠40只全部进入结果分析。①血清睾酮含量:模型组明显低于正常组,分别为(0.52±0.14),(1.26±0.32)μg/L(t=3.004,P<0.05);何首乌丸加味组为(1.16±0.32)μg/L,高于模型组(t=2.321,P<0.05)。②血清超氧化物歧化酶活性:模型组明显低于正常组,分别为(106.22±9.63),(148.73±12.93)kNU/L(t=13.339,P<0.01);何首乌丸加味组为(140.05±15.57)kNU/L,高于模型组(t=9.970,P<0.01)。③p53阳性细胞率:模型组明显高于正常组,分别为(59.13±10.53)%和(36.99±9.45)%,(χ2=9.696,P<0.01);何首乌丸加味组为(43.03±12.65)%,低于模型组(χ2=5.122,P<0.05)。结论:何首乌丸加味可通过提高睾酮含量、增强抗氧化能力、降低生精细胞的凋亡改善衰老大鼠睾丸的功能,起到延缓衰老的作用。

关键词：何首乌/药理学衰老睾丸基因 p53

The Study of Aperture Confocal Unstable Cavity (ACUC)

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光电子.激光 1989年第2期 90-93页

作者： Qiu Junlin, zh.ang zhongzhen, gong zhiwei,Sun Suhua, Tang zhonghua and Chen YihongLaser Institute of Huazhong Universitu of Scienceand Technology, Wuhan, Hubei, P.R.ChinaTelephone No. 870154 EXT 351

This paper illustrates the schematic diagram for ACUC and the physical process of its light wave transmission. The paper also presents the formula for calculation of the energy loss coefficient. Experiments have prove... 详细信息

关键词： The Study of Aperture Confocal Unstable Cavity ACUC

Biomechanical Analysis of Personalised 3D-Printed Clavicle Plates of Different Materials to Treat Midshaft Clavicle Fractures

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Journal of Shanghai Jiaotong university(Science) 2021年第3期26卷 259-266页

作者： CHENG Rongshan JIANG Ziang DIMITRIOU Dimitriou gong Weihua TSAI Tsung-Yuan School of Biomedical Engineering&Med-X Research Institute Shanghai Jiao Tong UniversityShanghai200030China Department of Orthopaedic Surgery Shanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghai200011China Department of Orthopedics Bürgerspital SolothurnSchöngrünstrasse 42CH-4500SolothurnSwitzerland

This study was aimed at comparing the biomechanical performance of personalised 3D-printed clavicle plates of different materials to treat midshaft clavicle fractures with the finite element(FE)*** FE model of a fractured clavicle with a personalised 3D-printed clavicle plate and screws was *** types of materials were simulated,including stainless steel,titanium alloy,and magnesium *** loading conditions(axial compression and inferior bending)were applied at the distal end of the clavicle to simulate arm *** stiffness,peak stress,and bone strain at the clavicle fracture site were measured and *** stiffness of the stainless steel clavicle plate was significantly greater than that of the titanium alloy clavicle *** stiffness of the magnesium alloy clavicle plate was similar to that of the intact clavicle;peak stress of the magnesium alloy clavicle plate was the lowest;thus,it had less stress-shielding effects on bone *** magnesium alloy clavicle plate was more likely to form bone by distributing proper strain at the clavicle fracture *** to the influence of different materials on the tensile strength,magnesium alloy clavicle plates might be preferred owing to their bionic stiffness in the treatment of patients with a low risk of *** patients who engage in contact sports,a titanium alloy clavicle plate might be more suitable.

关键词： personalised treatment 3D printing clavicle plate biomechanical analysis

Lotus seedpod-inspired internal vascularized 3D printed scaffold for bone tissue repair

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Bioactive Materials 2021年第6期6卷 1639-1652页

作者： Xiaoyu Han Mingjie Sun Bo Chen Qimanguli Saiding Junyue zhang Hongliang Song Lianfu Deng Peng Wang Weiming gong Wenguo Cui Department of Orthopedics Jinan Central HospitalShandong First Medical University&Shandong Academy of Medical Sciences105 Jiefang RoadLixia DistrictJinanShandong250013PR China Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases Shanghai Institute of Traumatology and OrthopedicsRuijin HospitalShanghai Jiao Tong University School of Medicine197 Ruijin 2nd RoadShanghai200025PR China

In the field of bone defect repair,3D printed scaffolds have the characteristics of personalized customization and accurate internal ***,how to construct a well-structured vascular network quickly and effectively inside the scaffold is essential for bone repair after ***,inspired by the unique biological structure of“lotus seedpod”,hydrogel microspheres encapsulating deferoxamine(DFO)liposomes were prepared through microfluidic technology as“lotus seeds”,and skillfully combined with a three-dimensional(3D)printed bioceramic scaffold with biomimetic“lotus”biological structure which can internally grow blood *** this composite scaffold system,DFO was effectively released by 36%in the first 6 h,which was conducive to promote the growth of blood vessels inside the scaffold *** the following 7 days,the release rate of DFO reached 69%,which was fundamental in the formation of blood vessels inside the scaffold as well as osteogenic differentiation of bone mesenchymal stem cells(BMSCs).It was confirmed that the composite scaffold could significantly promote the human umbilical vein endothelial cells(HUVECs)to form the vascular morphology within 6 h in *** vivo,the composite scaffold increased the expression of vascularization and osteogenic related proteins Hif1-α,CD31,OPN,and OCN in the rat femoral defect model,significantly cutting down the time of bone *** sum up,this“lotus seedpod”inspired porous bioceramic 3D printed scaffold with internal vascularization functionality has broad application prospects in the future.

关键词： 3D scaffolds Drug release Neovascularization Osteogenesis Regeneration

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显现之维——对“文”范畴的考察

烟台大学学报（哲学社会科学版） 2019年第4期32卷 66-76页

作者：李光柱烟台大学人文学院

一个“维度”就意味着一种敞开的可能性。“文”作为中国古代的政治理想和人格理想,其核心要义就在于敞开和显现。“山川之守”作为“王”的职责,意味着政治要让天地万物的存在得到彰显,意味着人与自然的共同实现,意味着“和”与“美”... 详细信息

一个“维度”就意味着一种敞开的可能性。“文”作为中国古代的政治理想和人格理想,其核心要义就在于敞开和显现。“山川之守”作为“王”的职责,意味着政治要让天地万物的存在得到彰显,意味着人与自然的共同实现,意味着“和”与“美”的世界理念;“光明崇拜”从一种宇宙哲学演化为对政治伦理维度的现实关切,这一关切最终在《尚书金鄊》中通过周公的仪式操作得以确立;“文”与“恭”的辩证关系彰显了“仁”的内涵及其现实行动指向;“诚”作为君子的人格理想,意味着自身品格的完善和潜力的开发,最终要在至高的政治理想中得到实现。在马克思劳动实践论的意义上,“文”意味着“合规律性与合目的性的统一”,意味着自由。

关键词：文存在《论语》《国语》《尚书》