Lotus seedpod-inspired internal vascularized 3D printed scaffold for bone tissue repair

作     者：Xiaoyu Han Mingjie Sun Bo Chen Qimanguli Saiding Junyue Zhang Hongliang Song Lianfu Deng Peng Wang Weiming Gong Wenguo Cui 

作者机构：Department of OrthopedicsJinan Central HospitalShandong First Medical University&Shandong Academy of Medical Sciences105 Jiefang RoadLixia DistrictJinanShandong250013PR China Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint DiseasesShanghai Institute of Traumatology and OrthopedicsRuijin HospitalShanghai Jiao Tong University School of Medicine197 Ruijin 2nd RoadShanghai200025PR China 

出 版 物：《Bioactive Materials》 (生物活性材料（英文）)

年 卷 期：2021年第6卷第6期

页      面：1639-1652页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1002[医学-临床医学] 08[工学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 0805[工学-材料科学与工程（可授工学、理学学位）] 0802[工学-机械工程] 0836[工学-生物工程] 080201[工学-机械制造及其自动化] 10[医学] 

基　　金：This work was supported by the National Key R&D Program of China(2019YFA0112000) National Natural Science Foundation of China(51873107) Shanghai Municipal Health and Family Planning Commission(201840027) Shanghai Jiao Tong University“Medical and Research”Program(ZH2018ZDA04) The Project Supported by the Foundation of National Facility for Translational Medicine(Shanghai)(TMSK-2020-117) 

主　　题：3D scaffolds Drug release Neovascularization Osteogenesis Regeneration 

摘      要：In the field of bone defect repair,3D printed scaffolds have the characteristics of personalized customization and accurate internal ***,how to construct a well-structured vascular network quickly and effectively inside the scaffold is essential for bone repair after ***,inspired by the unique biological structure of“lotus seedpod,hydrogel microspheres encapsulating deferoxamine(DFO)liposomes were prepared through microfluidic technology as“lotus seeds,and skillfully combined with a three-dimensional(3D)printed bioceramic scaffold with biomimetic“lotusbiological structure which can internally grow blood *** this composite scaffold system,DFO was effectively released by 36%in the first 6 h,which was conducive to promote the growth of blood vessels inside the scaffold *** the following 7 days,the release rate of DFO reached 69%,which was fundamental in the formation of blood vessels inside the scaffold as well as osteogenic differentiation of bone mesenchymal stem cells(BMSCs).It was confirmed that the composite scaffold could significantly promote the human umbilical vein endothelial cells(HUVECs)to form the vascular morphology within 6 h in *** vivo,the composite scaffold increased the expression of vascularization and osteogenic related proteins Hif1-α,CD31,OPN,and OCN in the rat femoral defect model,significantly cutting down the time of bone *** sum up,this“lotus seedpodinspired porous bioceramic 3D printed scaffold with internal vascularization functionality has broad application prospects in the future.