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Neuroprotective effects of rapamycin on spinal cord injury in rats by increasing autophagy and Akt signaling

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Neural Regeneration Research 2019年第4期14卷 721-727页

作者： Xi-gong li Jun-Hua Du Yang Lu Xiang-Jin lin Department of Orthopedic Surgery The First Affiliated Hospital School of Medicine Zhejiang University

Rapamycin treatment has been shown to increase autophagy activity and activate Akt phosphorylation, suppressing apoptosis in several models of ischemia reperfusion injury. However, little has been studied on the neuroprotective effects on spinal cord injury by activating Akt phosphorylation. We hypothesized that both effects of rapamycin, the increased autophagy activity and Akt signaling, would contribute to its neuroprotective properties. In this study, a compressive spinal cord injury model of rat was created by an aneurysm clip with a 30 g closing force. Rat models were intraperitoneally injected with rapamycin 1 mg/kg, followed by autophagy inhibitor 3-methyladenine 2.5 mg/kg and Akt inhibitor IV 1 μg/kg. Western blot assay, immunofluorescence staining and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay were used to observe the expression of neuronal autophagy molecule Beclin 1, apoptosis-related molecules Bcl-2, Bax, cytochrome c, casp ase-3 and Akt signaling. Our results demonstrated that rapamycin inhibited the expression of mTOR in injured spinal cord tissue and up-regulated the expression of Beclin 1 and phosphorylated-Akt. Rapamycin prevented the decrease of bcl-2 expression in injured spinal cord tissue, reduced Bax, cytochrome c and caspase-3 expression levels and reduced the number of apoptotic neurons in injured spinal cord tissue 24 hours after spinal cord injury. 3-Methyladenine and Akt inhibitor IV intervention suppressed the expression of Beclin-1 and phosphorylated-Akt in injured spinal cord tissue and reduced the protective effect of rapamycin on apoptotic neurons. The above results indicate that the neuroprotective effect of rapamycin on spinal cord injury rats can be achieved by activating autophagy and the Akt signaling pathway.

关键词： nerve regeneration rapamycin mammalian target of rapamycin,mTOR autophagy Beclin 1 3-methyladenine acute spinal cord injury apoptosis Bax Akt neural regeneration

Probiotic Paenibacillus polymyxa 10 and Lactobacillus plantarum 16 enhance growth performance of broilers by improving the intestinal health

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Animal Nutrition 2021年第3期7卷 829-840页

作者： Baikui Wang li gong Yuanhao Zhou li Tang Zihan Zeng Qi Wang Peng Zou Dongyou Yu Weifen li Key Laboratory of Molecular Animal Nutrition of the Ministry of Education Key Laboratory of Animal Nutrition and Feed Science(Eastern of China)of the Ministry of AgricultureKey Laboratory of Animal Feed and Nutrition of Zhejiang ProvinceInstitute of Animal Nutrition and Feed SciencesCollege of Animal SciencesZhejiang University310058HangzhouChina Hainan Institute Zhejiang UniversitySanya 572000China

With the ever-growing strict prohibitions on antibiotic growth promoters(AGP)in animal production,in-feed probiotics are becoming attractive alternatives to antibiotics in the poultry *** investigate the effects of Paenibacillus polymyxa 10 and Lactobacillus plantarum 16 on the growth performance and intestinal health of broilers,540 male Cobb 500 broilers of 1 d old were randomly divided into 3 groups with 6 replicates per group and 30 chicks per *** were fed with either a basal diet or basal diets supplemented with 1×108 colony-forming units(CFU)/kg *** 10(BSC10)or *** 16(Lac16)for 42 *** showed that Lac16 treatment improved(Plightly improved(P>0.05)the growth performance of the starter phase *** increased villus height(Plial *** probiotic treatments up-regulated(Plicaecorum,Faecalibacterium prausnitzii,Lachnospira and Cop-rococcu,and significantly(Pli,Bacteroides fragilis and Shigella sonnei).Furthermore,the 2 probiotic treatments also increased the positive connection among the intestinal microbes and the carbohydrate metabolism-related pathways of the intes-tinal bacteria(P<0.05),with decreasing(P<0.05)nucleotides biosynthesis-related pathways of the intesti

关键词： Paenibacillus polymyxa Lactobacillus plantarum Growth performance Intestinal health Broiler

Characterizing Post-PKS Modifications of 16-Demethyl-rifamycin Revealed Two Dehydrogenases Diverting the Aromatization Mode of Naphthalenic Ring in Ansamycin Biosynthesis

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Chinese Journal of Chemistry 2022年第13期40卷 1553-1558页

作者： Ting-Yan Han Kai Zhang gong-li Tang Qiang Zhou State Key Laboratory of Bio-organic and Natural Products Chemistry Shanghai Institute of Organic ChemistryChinese Academy of Sciences345 Lingling RoadShanghai 200032China School of Chemistry and Materials Science Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of Sciences1Sublane XiangshanHangzhouZhejiang 310024China

The anti-tuberculous rifamycins belong to naphthalenic ansamycin based on the structure of aromatic ***,we explored the post-polyketide synthase(PkS)modifications in the biosynthesis of 16-demethyl-rifamycins via gene knockout,complementation and in vitro enzyme *** collective evidences showed that i)the aromatization of 8-hydroxyl-7,8-dihydronaphtoquinone was accomplished by the combined action of two dehydrogenases,Rifs and Rif;i)the acetylation and methylation of the macrocycle was carried out on naphthoquinone intermediates in preference to naphthol,by Rif-Orf20 and Rif-Orf14,respectively ii)the presence of Rifs/T homologs in ansamycin biosynthetic gene clusters corresponds to the dehydrogenation aromatization mode of *** findings cast new insights into the naphthalene formation and post-PkS modification of ansamycins.

关键词： Ansamycin Natural products Biosynthesis Dehydrogenation Aromatization

Two complete mitogenomes of Ocypodoidea(Decapoda: Brachyura), Cleistostoma dilatatum(Camptandriidae) and Euplax sp.(Macrophthalmidae) and its phylogenetic implications

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Acta Oceanologica Sinica 2023年第4期42卷 81-92页

作者： Ying Zhang liming Wei Bingjian liu liqin liu Zhenming Lü li gong National Engineering Laboratory of Marine Germplasm Resources Exploration and Utilization National Engineering Research Center for Facilitated Marine AquacultureZhoushan 316022China Marine Science and Technology College Zhejiang Ocean UniversityZhoushan 316022China

Complete mitochondrial genomes(mitogenomes) can provide useful information for phylogenetic relationships,gene rearrangement, and molecular evolution. In the present study, two newly sequenced mitogenomes of Ocypodoidea(Cleistostoma dilatatum and Euplax sp.) were reported for the first time, which are 15 444 bp and16 129 bp in length, respectively. Cleistostoma dilatatum is the first species in the family Camptandriidae whose complete mitogenome was sequenced. Each mitogenome contains an entire set of 37 genes and a putative control region, but their gene arrangements are largely different. Tandem duplication and random loss model is proposed to account for their gene arrangements. Comparative genomic analyses of 19 mitogenomes clustering in one branch reveal that 18 of them shared the same gene rearrangement, while that of C. dilatatum mitogenome was consistent with the ancestral gene arrangement of Brachyura. The dN/dS ratio analysis shows that all PCGs are evolving under purifying selection. Phylogenetic analyses show that all Macrophalmidae species cluster together as a group, and then form a sister clade with Camptandriidae. Moreover, the polyphyly of three superfamilies(Ocypodoidea, Eriphioidea, and Grapsoidea) is reconfirmed. These findings help to confirm the phylogenetic position of Camptandriidae, as well as provide new insights into the phylogeny of Brachyura.

关键词： Camptandriidae Macrophthalmidae mitogenome gene rearrangement phylogenetic analysis

A new flavonol glycoside from Hydrangea macrophylla (Thunb.) Seringe

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Chinese Chemical Letters 2010年第6期21卷 690-692页

作者： Wei Sheng Feng Yan li Zhang Xiao Ke Zheng Bo Zhu li gong Yang Zhen li Henan College of Traditional Chinese Medicine Zhengzhou 450008China

A new kaempferol glycoside,named kaempferol 3-O-[6″-O-β-D-glucopyranosyl-6′′′-O-α-L-rhamnopy ranosyl-2′′′′-O-α-L-rhamnopyranosyl]-β-D-glucopyranoside,was isolated from Hydrangea macrophylla(Thunb.) *** str... 详细信息

关键词： Hydrangea macrophylla (Thunb.) Seringe Kaempferol glycoside

Frequent recombination in Cynoglossus abbreviatus(Pleuronectiformes:Cynoglossidae)ribosomal 18S rDNA

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Acta Oceanologica Sinica 2024年第8期43卷 98-103页

作者： li gong Tingqi Jiang Bilin Hu Kaixin Wang Nannan Zhang Zengliang Miao National Engineering Laboratory of Marine Germplasm Resources Exploration and Utilization Zhejiang Ocean UniversityZhoushan 316022China Marine Science and Technology College Zhejiang Ocean UniversityZhoushan 316022China

The conventional theory of concerted evolution has been used to explain the lack of sequence variation in ribosomal RNA(rRNA)genes across diverse eukaryotic ***,recent investigations into rRNA genes in flatfish genome have resulted in controversial *** study focuses on 18S rRNA genes of the widely distributed tongue sole,Cynoglossus abbreviatus(Pleuronectiformes:Cynoglossidae),aiming to explore sequence *** distinct 18S rDNA sequence types(Type A,B,R1,R2,and R3)were identified,suggesting a departure from concerted evolution.A combination of general criteria and variations in highly conserved regions were employed to detect *** results pinpointed Type A sequences as potential pseudogenes due to significant sequence variations and deviations in secondary structure within highly conserved *** types(Type R1,R2,and R3)were identified as recombinants between Type A and B sequences,with simple crossing over and gene conversion as the most likely recombination *** findings not only contribute to rRNA pseudogene identification but also shed light on the evolutionary dynamics of rRNA genes in teleost genomes.

关键词： ribosomal RNA tongue sole non-concerted evolution pseudogene crossing over gene conversion

Polyamide Nanofiltration Membrane from Surfactant-assembly Regulated Interfacial Polymerization of 2-Methylpiperazine for Divalent Cations Removal

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Chemical Research in Chinese Universities 2022年第3期38卷 782-789页

作者： gong li ZHU Yuzhang DONG Dianyu ZHANG Yuping GUI liangliang JIN Jian College of Chemistry Chemical Engineering and Materials ScienceSoochow UniversitySuzhou215123P.R.China i-Lab Suzhou Institute of Nano-Tech and Nano-BionicsChinese Academy of SciencesSuzhou215123P.R.China

Removal of metal ions from water can not only alleviate the scaling problem of domestic and industrial water,but also solve the water safety problem caused by heavy metal ion ***,we fabricate a positively charged nanofiltration membrane via surfactant-assembly regulated interfacial polymerization(SARIP)of 2-methylpiperazine(MPIP)and trimesoyl chloride(TMC).Due to the existence of methyl substituent,MPIP has lower reactive activity than piperazine(PIP)but stronger affinity to hexane,resulting in a nanofiltration(NF)membrane with an opposite surface charge and a loose polyamide active ***,with the help of sodium dodecyl sulfate(SDS)assembly at the water/hexane,the reactivity between MPIP and TMC was obviously increased and caused in turn the formation of a positively charged polyamide active layer with a smaller pore size,as well as with a narrower pore size *** resulting membrane shows a highly efficient removal of divalent cations from water,of which the rejections of MgCl_(2),CoCl_(2)and NiCl_(2)are higher than 98.8%,98.0%and 98.0%,respectively,which are better than those of most of other positively charged NF membranes reported in literatures.

关键词： Nanofiltration membrane Interfacial polymerization Surface charge Divalent cation removal

肺栓塞诊断中多层螺旋CT双期动态增强扫描技术的应用研究

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临床研究 2025年第1期33卷 133-135页

作者：柴清华杨瑞刘继伟弓莉河南省胸科医院医学影像科河南郑州450000

目的分析研究肺栓塞诊断中应用多层螺旋CT双期动态增强扫描技术的应用价值。方法选取河南省胸科医院2020年2月至2022年6月收治的50例疑似肺栓塞患者,选择SOMATOM Definition Flash双源CT扫描机开展胸部平扫和肺动脉期、肺静脉期双期... 详细信息

目的分析研究肺栓塞诊断中应用多层螺旋CT双期动态增强扫描技术的应用价值。方法选取河南省胸科医院2020年2月至2022年6月收治的50例疑似肺栓塞患者,选择SOMATOM Definition Flash双源CT扫描机开展胸部平扫和肺动脉期、肺静脉期双期动态增强CT扫描,分析双期动态增强扫描图像;以综合评定结果为金标准,计算双期动态增强CT扫描检查结果。结果分析多层螺旋CT双期动态增强扫描图像发现,41例患者诊断为肺栓塞,能精确发现145处栓子,其中13处为左右肺动脉主干,57处为叶动脉,55处为段动脉,18处为亚段动脉,2处为亚亚段动脉。50例患者经综合评估,结果为阳性48例,阴性2例,双期动态增强CT扫描检查结果为阳性46例,阴性4例。双期动态增强CT扫描检查敏感度为95.83%(46/48),特异度为100.00(2/2),准确度为96.00%(48/50)。结论临床诊断肺栓塞时采用多层螺旋CT双期动态增强扫描技术具有显著的检测和诊断效果,准确性、特异性及敏感度均较高,值得应用。

关键词：肺栓塞多层螺旋CT 双期动态增强扫描技术应用价值

Effect of annealing and cooling rate on toughness of G115 heat-resistant steels

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Journal of Iron and Steel Research International 2022年第8期29卷 1245-1256页

作者： Zhen liu li gong Chi Zhang Zheng-dong liu Zheng-zong Chen Yun-he Yu Key Laboratory for Advanced Materials of Ministry of Education School of Materials Science and EngineeringTsinghua UniversityBeijing 100084China Institute for Special Steels China Iron and Steel Research InstituteBeijing 100081China

The effects of annealing before normalizing and different cooling ways, i.e., air cooling, quenching, and water mist cooling after normalizing on the toughness of G115 steels were investigated. The impact tests showed that the annealed samples had better toughness compared to the unannealed samples for three cooling ways. Microstructure observations revealed that the annealed samples had a more uniform grain distribution, smaller size and area fraction of M_(23)C_(6) particles along the grain boundaries, and lower dislocation density than those in the unannealed samples, which performed together for good toughness. Among three cooling ways, air cooling gave the best toughness due to the smallest occupancy of M23C6 particles in the grain boundaries. Thus, the combination of annealing conducted before normalizing and air cooling selected after normalizing can improve the toughness a lot of G115 steels.

关键词： G115 steel Annealing M_(23)C_(6)particle Dislocation density Toughness