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Fern extracts potentiate fluconazole activity and inhibit morphological changes in Candida species

Asian Pacific Journal of Tropical biomedicine 2017年第11期7卷 1025-1030页

作者： maria A.Freitas Antonia T.L.Santos Antonio J.T.machado Ana Raquel P.Silva Fábia F.Campina maria S.Costa gioconda m.a.b.martins maria Flaviana b.morais-braga Saulo R.Tintino Irwin R.A.menezes Jaime Ribeiro-Filho Altevir P.medeiros Adeliana S.Oliveira Patrício b.maracajá Henrique D.m.Coutinho Universidade Regional do Cariri-URCA Universidade Federal Rural do Semi-A′rido-UFERSA Universidade Federal do Rio Grande do Norte-UFRN Universidade Federal de Campina Grande-UFCG

Objective: To investigate the antifungal activity of the fern species Lygodium venustum(L. venustum) and Pityrogramma calomelanos(P. calomelanos) against Candida albicans and Candida tropicalis ***: The microdilution method was used to evaluate the antifungal activity, as well as the modulating effects of ethanolic extracts of these plants in combination with fluconazole. The minimum inhibitory concentration(mIC), minimum fungicide concentration and morphological changes were also ***: The extract obtained from L. venustum presented a mIC > 8 192 mg/m L, while the extract obtained from and P. calomelanos presented a mIC = 8 192 mg/m L, indicating that they present weak antifungal activity. However, combination of the extracts with Fluconazole potentiated the antifungal activity of this drug. At different experimental conditions, such as concentration of the extract and type of strain, the extracts inhibited hyphae and pseudohyphae formation, indicating that these fern species can affect the morphology of the ***: The extracts obtained from the fern species L. venustum and P. calomelanos dose not present significant antifungal activity. However, P. calomelanos potentiates the activity of fluconazole and both extracts inhibits the morphological changes in Candida species, indicating that they have potential pharmacological activity as modulators of fungal biology. Therefore, novel studies are required to characterize the interference of these extracts in the virulence and pathogenicity of Candida species as well as the potential of fern species to treat fungal infections.

关键词： Dimorphism Candida species Ferns Lygodium venustum Pityrogramma calomelanos

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Global prevalence and gender inequalities in at least 60 min of self-reported moderate-to-vigorous physical activity 1 or more days per week:An analysis with 707,616 adolescents

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Journal of Sport and Health Science 2024年第5期13卷 709-716页

作者： Raphael H.O.Araujo AndrúO.Werneck Clarice L.martins Luciana L.barboza Rafael m.Tassitano Nicolas Aguilar-Farias Gilmar m.Jesus Robinson Ramírez-Véelez Riki Tesler Adewale L.Oyeyemi Ellen C.m.Silva Robert G.Weaver mark S.Tremblay Javier brazo-Sayavera Grégore I.mielke Danilo R.P.Silva Graduation Program in Health Sciences Londrina State University(UEL)Londrina 86057-970Brazil Center for Epidemiological Research in Nutrition and Health Department of NutritionSchool of Public HealthUniversidade de Sao Paulo(USP)Sao Paulo 01246-904Brazil Department of Physical Education Federal University of ParaıbaParaıba 58051-900Brazil Research Centre of Physical Activity Health and LeisureFaculty of SportsLaboratory for Integrative and Translational Research in Population Health(ITR)University of PortoPorto 4500Portugal Postgraduate Program in Physical Education University of BrasıliaBrasılia 70910-900Brazil Department of Kinesiology and Community Health University of Illinois at Urbana-ChampaignChampaignIL 61801-3841USA Department of Physical Education Sports and RecreationUniversidad de La FronteraTemuco 4680000Chile Department of Health State University of Feira de SantanaFeira de Santana 44036-900Brazil Navarrabiomed Hospital Universitario de Navarra(HUN)Navarra Institute for Health Research(IdiSNA)Universidad Publica de Navarra(UPNA)Pamplona 31006Spain Facultad de Ciencias de la Educacion Unidad Central del Valle del Cauca(UCEVA)TuluaValle del Cauca 763022Colombia Health management Department Faculty of Heath sciencesAriel UniversityAriel 40700Israel College of Health Solutions Arizona State UniversityPhoenixAZ 85004USA Arnold School of Public Health University of South CarolinaColumbiaSC 29208USA Healthy Active Living and Obesity Research Group Children’s Hospital of Eastern Ontario Research InstituteOttawaON K1H 8L1Canada Department of Pediatrics University of OttawaOttawaON K1H 8L1Canada Department of Health Sciences Carleton UniversityOttawaON K1S 5B6Canada Department of Sports and Computer Science Universidad Pablo de Olavide(UPO)Seville 41013Spain Centro Universitario Regional Noreste Universidad de la RepublicaRivera 40000Uruguay School of Public Health The University of QueenslandBrisbaneQLD 4006Australia Department of Physical Education

background:Guidelines recommend that adolescents should accumulate an average of 60 min per day of moderate-to-vigorous physical activity(mVPA).However,using only this cut-off could hide important *** instance,from a population-level point of view,increasing physical activity for those with no or low physical activity could provide more health benefits than increasing physical activity for those with intermediate ***,including a more sensitive cut-point of≥1 days per week could be an additional strategy for identifying those with low access/opportunities for physical activity ***,the current study aims to estimate the prevalence of≥60 min of mVPA≥1 days per week among adolescents globally,and to describe any relevant gender ***:We used representative datasets from 146 countries/territories collected between 2003 and *** was *** were grouped into younger(≤14 years old)and older(>14 years old)*** Poisson regression models were used to identify the relative differences in≥60 min of mVPA≥1 days per week between boys and girls,and random-effects meta-analysis models were used to identify the pooled *** were stratified by country and ***:Approximately 80%of both younger and older adolescents reported≥60 min of mVPA≥1 days per *** prevalence was≥94%in Europe and Central Asia and North America,while the estimates for the other regions weremin of mVPA≥1 days per week was higher among boys than girls,with the largest differences occurring among the oldest adolescents(Prevalence ratio_(≤14y)=1.04(95%confidence interval(95%CI)):1.03-1.04)*** ratio_(>14y)=1.09(95%CI:1.08-1.10)).Conclusion:Approximately 8 out of 10 adolescents reported accumulating≥60 min of mVPA≥1 days per week,with notable differences between *** differences were observed in several countries,especially among the oldest *** fo

关键词： Global School-based Student Health Survey Health behaviour Among School-aged Children survey Inequality Surveillance Youth

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Antiglycation,antioxidant,antiacne,and photoprotective activities of crude extracts and triterpene saponin fraction of Sapindus saponaria ***:An in vitro study

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Asian Pacific Journal of Tropical biomedicine 2022年第9期12卷 391-399页

作者： Regildo m.G.Silva Gustavo R.martins Laura m.b.Nucci Filipe O.Granero Célia C.m.Figueiredo Patrícia S.Santiago Luciana P.Silva Laboratory of Herbal medicine and Natural Products Department of BiotechnologyFaculty of SciencesHumanities and LanguagesSão Paulo State UniversityAssisSão PauloBrazil Institute of Chemistry São Paulo State UniversityAraraquaraSão PauloBrazil São Paulo State University Campus de RegistroRegistroSão PauloBrazil Fundação Educacional do município de Assis AssisSão PauloBrazil

Objective:To evaluate the photoprotective,antioxidant,antiglycation,and antiacne activities of crude extract(CESs)and triterpene saponin fraction(TSSs)of Sapindus ***:HPLC-mS purification was performed on a Symmetry Tm C18 *** saponins were identified by a UV *** activity was evaluated by DPPH and O_(2)^(−)radicals scavenging,and FRAP and TbARS *** activity was assessed by relative electrophoretic mobility and inhibition of advanced glycation end products(AGEs)***,antiacne activity was determined by inhibition of Cutibacterium acnes,and photoprotective effect was evaluated by mansur’s ***:most of the triterpene saponins detected in the fraction by HPLC-mS analysis were hederagenin as the *** and TSSs presented varying antioxidant activity in DPPH(CESs:75.69%and TSSs:83.65%),FRAP(CESs:425.39μm TE/g DW and TSSs:649.36μm TE/g DW),TbARS(CESs:42.96%and TSSs:52.16%)and O_(2)^(−)radicals scavenging(CESs:61.33%and TSSs:86.69%)*** and TSSs also exhibited antiglycation activity comparable to bovine serum albumin treated with *** addition,CESs and TSSs showed inhibition of AGE formation(34.48%and 61.85%,respectively).Antiacne activity against Cutibacterium acnes was observed with a minimum inhibitory concentration equal to minimum bactericidal concentration(CESs:36.11µg/mL and TSSs:18.34µg/mL).In photoprotective assays,CESs and TSSs showed maximum absorbance of 1.42 to 0.20 and 2.80 to 1.30,respectively,in the wavelength range of 260 to 400 ***,CESs and TSSs showed sun protection factors of 8.89 and 14.89,***:Sapindus saponaria fruit extracts show strong antioxidant potential and antiglycation activity against bovine serum albumin glycation and AGE ***,they presented antibacterial activity against Cutibacterium acnes and photoprotective effect against UV-A and UV-b.

关键词： Photoprotective AGE formation Free radical Antioxidant Triterpene saponin Sapindus saponaria

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Photobiomodulation therapy assisted orthodontic tooth movement:potential implications,challenges,and new perspectives

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Journal of Zhejiang University-Science b(biomedicine & biotechnology) 2023年第11期24卷 957-973页

作者： Jiawen YONG Sabine GROGER Julia VON bREmEN marcia martins mARQUES Andreas bRAUN Xiaoyan CHEN Sabine RUF Qianming CHEN Stomatology Hospital School of StomatologyZhejiang University School of MedicineZhejiang Provincial Clinical Research Center for Oral DiseasesKey Laboratory of Oral Biomedical Research of Zhejiang ProvinceCancer Center of Zhejiang UniversityEngineering Research Center of Oral Biomaterials and Devices of Zhejiang ProvinceHangzhou310000China Department of Orthodontics Faculty of MedicineJustus Liebig University GiessenGiessen35392Germany School of Dentistry University of Sao PauloSao Paulo05508000Brazil Department of Operative Dentistry Periodontology and Preventive DentistryRWTH Aachen UniversityAachen52074Germany

Over the past decade,dramatic progress has been made in dental research areas involving laser *** photobiomodulatory effect of laser light regulates the behavior of periodontal tissues and promotes damaged tissues to heal ***,photobiomodulation therapy(PbmT),a non-invasive treatment,when applied in orthodontics,contributes to alleviating pain and reducing inflammation induced by orthodontic forces,along with improving tissue healing ***,PbmT is attracting more attention as a possible approach to prevent the incidence of orthodontically induced inflammatory root resorption(OIIRR)during orthodontic treatment(OT)due to its capacity to modulate inflammatory,apoptotic,and anti-antioxidant ***,a systematic review revealed that PbmT has only a moderate grade of evidence-based effectiveness during orthodontic tooth movement(OTm)in relation to OIIRR,casting doubt on its beneficial *** PbmT-assisted orthodontics,delivering sufficient energy to the tooth root to achieve optimal stimulation is challenging due to the exponential attenuation of light penetration in periodontal *** penetration of light to the root surface is another crucial unknown *** the penetration depth and distribution of light in periodontal tissues are ***,advanced approaches specific to orthodontic application of PbmT need to be established to overcome these *** review explores possibilities for improving the application and effectiveness of PbmT during *** aim was to investigate the current evidence related to the underlying mechanisms of action of PbmT on various periodontal tissues and cells,with a special focus on immunomodulatory effects during OTm.

关键词： Photobiomodulation therapy Low-level laser therapy(LLLT) Low-intensity laser therapy(LILT) Orthodontic tooth movement Orthodontics Immunorthodontics

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Finite element model with imposed slip surfaces for earth mass safety evaluation

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Journal of Central South University 2014年第9期21卷 3627-3638页

作者： Nuno ARAUJO Gaspar J.mACHADO Julio b.martins Hugo m.R.D.SILVA C-TAC Territory Environment and Construction Research Centre School of EngineeringUniversity of Minho Guimaraes 4800-058 Portugal Centre of mathematics School of Sciences University of Minho Guimaraes 4800-058 Portugal

The study of earth masses requires numerical methods that provide the quantification of the safety factor without requiring detrimental assumptions. For that, equilibrium analysis can perform fast computations but require assumptions that limit its potentiality. Limit analysis does not require detrimental assumptions but are numerically demanding. This work provides a new approach that combines the advantage of both the equilibrium method and the limit analysis. The defined hybrid model allows probabilistic analysis and optimization approaches without the assumption of interslice forces. It is compared with a published case and used to perform probabilistic studies in both a homogeneous and a layered foundation. Analyses show that the shape of the density probability functions is highly relevant when computing the probability of failure, and soil elasticity hardly affects the safety of factor of the earth mass.

关键词： slope stability probabilistic methods reliability genetic algorithms safety factor

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P2X7 receptor blockade decreases inflammation,apoptosis,and enteric neuron loss during Clostridioides difficile toxin A-induced ileitis in mice

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World Journal of Gastroenterology 2022年第30期28卷 4075-4088页

作者： Ana A Q A Santos Deiziane V S Costa Danielle A Foschetti Antoniella S G Duarte Conceicao S martins Pedro m G Soares Patricia Castelucci Gerly A C brito Department of morphology School of MedicineFederal University of CearaFortaleza 60430-170CearaBrazil Department of Physiology and Pharmacology School of MedicineFederal University of CearáFortaleza 60430-170CearaBrazil Department of Pathology and Legal medicine School of MedicineFederal University of CearaFortaleza 60430-170CearaBrazil Department of morphology(UFC) Federal University of CearaFortaleza 60430-170CearaBrazil Department of Anatomy Institute of Biomedical SciencesUniversity of São PauloSao Paulo 05508-270Brazil Department of morphology Federal University of CearaFortaleza 60140-170CearaBrazil

Clostridioides difficile(***)is the most common pathogen causing health care-associated *** TcdA and Tcdb have been shown to activate enteric neurons;however,what population of these cells is more profoundly influenced and the mechanism underlying these effects remain *** To characterize a specific population of TcdA-affected myenteric neurons and investigate the role of the P2X7 receptor in TcdA-induced ileal inflammation,cell death,and the changes in the enteric nervous system in *** Swiss mice were used to model TcdA-induced ileitis in ileal loops exposed to TcdA(50μg/Loop)for 4 *** investigate the role of the P2X7 receptor,brilliant blue G(50 mg/kg,i.p.),which is a nonspecific P2X7 receptor antagonist,or A438079(0.7μg/mouse,i.p.),which is a competitive P2X7 receptor antagonist,were injected one hour prior to TcdA *** samples were collected to analyze the expression of the P2X7 receptor(by quantitative real-time polymerase chain reaction and immunohistochemistry),the population of myenteric enteric neurons(immunofluorescence),histological damage,intestinal inflammation,cell death(terminal deoxynucleotidyltransferasemediated dUTP-biotin nick end labeling),neuronal loss,and S100b synthesis(immunohistochemistry).RESULTS TcdA upregulated(Pmyenteric neurons compared to that in control *** with the control mice indicated that TcdA promoted(Pmyenteric calretinin+(Calr)and choline acetyltransferase+neurons and increased the number of nitrergic+and Calr+neurons expressing the P2X7 *** of the P2X7 receptor decreased TcdAinduced intestinal damage,cytokine release[interleukin(IL)-1β,IL-6,IL-8,and tumor necrosis factor-α],cell death,enteric neuron loss,and S100b synthesis in the mouse *** Our findings demonstrated that TcdA induced the upregulation of the P

关键词： Clostridioides difficile Clostridioides difficile toxin A P2X7 receptor Enteric nervous system Enteric neuron Enteric glia

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AbA signaling prevents phosphodegradation of the SR45 splicing factor to alleviate inhibition of early seedling development in Arabidopsis

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Plant Communications 2023年第2期4卷 272-285页

作者： Rui Albuquerque-martins Dora Szakonyi James Rowe Alexander m.Jones Paula Duque Instituto Gulbenkian de Ciencia 2780-156 OeirasPortugal Sainsbury Laboratory University of CambridgeCambridge B21LRUK

Serine/arginine-rich(SR)proteins are conserved splicing regulators that play important roles in plant stress responses,namely those mediated by the abscisic acid(AbA)*** Arabidopsis thaliana SR-like protein SR45 is a described negative regulator of the AbA pathway during early seedling *** the inhibition of growth by AbA signaling is counteracted to maintain plant development under stress conditions remains largely ***,we show that SR45 overexpression reduces Arabidopsis sensitivity to AbA during early seedling *** and confocal microscopy analyses of transgenic plants expressing fluorescently tagged SR45 revealed that exposure to AbA dephosphorylates the protein at multiple amino acid residues and leads to its accumulation,due to SR45 stabilization via reduced ubiquitination and proteasomal *** phosphomutant and phosphomimetic transgenic Arabidopsis lines,we demonstrate the functional relevance of AbA-mediated dephosphorylation of a single SR45 residue,T264,in antagonizing SR45 ubiquitination and degradation to promote its function as a repressor of seedling AbA *** results reveal a mechanism that negatively autoregulates AbA signaling and allows early plant growth under stress via posttranslational control of the SR45 splicing factor.

关键词： abscisic acid alternative splicing Arabidopsis thaliana protein phosphorylation SR proteins

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Effect of Combined Use of brackish Water and Nitrogen Fertilizer on biomass and Sugar Yield of Sweet Sorghum

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Pedosphere 2012年第6期22卷 785-794页

作者： T.b.RAmOS N.L.CASTANHEIRA m.C.GONALVES m.L.FERNANDES m.I.JANURIO m.E.LOURENO F.P.PIRES J.C.martins CEER-biosystems Engineering Institute of Agronomy Technical University of Lisbon Tapada da Ajuda 1349-017 Lisbon (Portugal) Estao Agronómica Nacional L-INIA Instituto Nacional de Recursos Biológicos Quinta do Marquês Av. República 2784-505 Oeiras (Portugal) Departamento de Fitotecnia Instituto de Ci encias Agrrias e Ambientais Mediterrnicas Universidade de vora Apartado 94 7002-554 vora (Portugal)

Soil salinization and non-point source pollution are among the most important and widespread environmental problems in European mediterranean regions. Sweet sorghum (Sorghum bicolor (L.) moench var. saccharatum) is a moderate to high salinity tolerant crop with low water and nutrient needs, seen as an alternative to grow in the water scarce regions. A three-year multifactorial study was conducted in southern Portugal to evaluate the combined effects of saline water and nitrogen application on the dry biomass (total, stems, and leaves), sugar content (total reducing sugars and sucrose contents), and sugar yield (here defined as the product of total reducing sugars and stems dry biomass) functions of sweet sorghum. Sorghum dry biomass and sugar yield showed diminishing returns for each incremental change of nitrogen. The use of saline irrigation waters also led to yield reduction. Exception was sucrose content which increased with increasing levels of sodium in the soil. Nitrogen need decreased as the amount of sodium applied increased. Stem dry biomass, sucrose content, and sugar yield progressively increased with progress in the experiment. The effect could be attributed to the increase of the amount of irrigation applied throughout the years, thus increasing the leaching fraction which promoted salt leaching from the root zone, reduced the salinity stress, increased plant transpiration, nitrogen uptake and biomass yield.

关键词：甜高粱产糖量生物量氮肥微咸水土壤盐渍化蔗糖含量生物产量

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First Late Triassic Record of a Paleoentomofauna from South America(malargüe basin,mendoza Province,Argentina)

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Acta Geologica Sinica(English Edition) 2010年第4期84卷 915-924页

作者： Carsten bRAUCKmANN Oscar F.GALLEGO Norbert HAUSCHKE Rafael G.martins-NETO Elke GROENING Jan-m.ILGER maría b.LARA Geology and Palaeontology Clausthal University of TechnologyLeibnizstrasse 10D-38678 Clausthal-ZellerfeldGermany micropaleontology(Faculty of Natural Sciences-National Northeast University) 3400 Corrientes-Argentina Palaeontology Area(Center of Applied Ecology of the Litoral-National Research Council) 3400 Corrientes-Argentina Geosciences Martin Luther University Halle-WittenbergVon-Seckendorff-Platz 3D-06120 Halle(Saale)Germany Federal University from Ceara Cariri/FUNCAPPresident Brazilian Paleoarthropodological SocietyBrazil

Late middle Triassic to early Late Triassic insects from Argentina have been previously described from the bermejo and Cuyana basins where they have been recovered from the Ischichuca-Los Rastros and Potrerillos-Cacheuta Formations, respectively. The insect fauna discussed herein was collected during field studies in 1986/1987 from the Llantenes section （Norian to Rhaetian？ Late Triassic）, which is situated in the malargiie basin in southern mendoza province. The insect remains were found in the upper part of the Llantenes section （Llantenes Formation）, which is built up of two coarsening-upwards cycles reflecting a deltaic progradation of a fluvial into a lacustrine environment （lower part）, succeeded by repeated progradatious into a floodplaindominated environment （upper part; with finds of insects, conchostracans, fish remains, plant fragments, and drifted logs）. The new finds represent the youngest Triassic insect records described from Argentina and even from South America in its entirety. There is only one contemporaneous fossil assemblage in Gondwana： in the Ciarence/moreton basin （Aberdare Conglomerate; Late Norian） in Australia. The new Triassic insects include an impression of an isolated mecopterida-like wing （mendozachorista volkheimeri gen. et sp. nov.; mendozachoristidae fam. nov.）, coleopteran elytra of the Permosynidae （Ademosyne rosenfeldi sp. nov. and Ademosyne Uantenesensis sp. nov.） and other isolated body fragments. This new Late Triassic entomofauna from Argentina is of considerable importance in the reconstruction of the biotic recovery of continental environments in Gondwana after the catastrophic mass extinction at the P/T boundary.

关键词： mecopterida Coleoptera Late Triassic Liantenes Formation malargiie basin Argentina

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A Pt/mnV_(2)O_(6) nanocomposite for the borohydride oxidation reaction

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Journal of Energy Chemistry 2021年第4期30卷 428-436页

作者： Jadranka milikic marta martins Ana S.Dobrota Gamze bozkurt Gulin S.P.Soylu Ayse b.Yurtcan Natalia V.Skorodumova Igor A.Pasti biljana Sljukic Diogo m.F.Santos Faculty of Physical Chemistry University of BelgradeStudentski trg 12-1611158 BelgradeSerbia CeFEmA Instituto Superior TécnicoUniversidade de LisboaLisbonPortugal Project Coordination Implementation and Research Center Erzurum Technical UniversityErzurumTurkey Faculty of Engineering Chemical Engineering DepartmentIstanbul University-Cerrahpasa34320 AvcilarIstanbulTurkey Department of Chemical Engineering Atatürk UniversityErzurumTurkey Department of materials Science and Engineering School of Industrial Engineering and ManagementKTH-Royal Institute of TechnologyBrinellvägen 2310044 StockholmSweden Department of Physics and Astronomy Uppsala UniversityBox 51675120 UppsalaSweden

Problems associated with carbon support corrosion under operating fuel cell conditions require the identification of alternative supports for platinum-based nanosized *** supported on manganese vanadate(Pt/mnV_(2)O_(6))was prepared by microwave irradiation method and characterized using X-ray diffraction,Fourier-transform infrared spectroscopy,X-ray photoelectron spectroscopy,scanning electron microscopy with energy dispersive spectroscopy,and transmission electron *** borohydride oxidation reaction(bOR)on Pt/mnV_(2)O_(6) was studied in highly alkaline media using voltammetry,chronoamperometry,and electrochemical impedance *** electrocatalytic activity of Pt/mnV_(2)O_(6) was also compared with that of commercial Pt/C(46 wt%Pt)*** apparent activation energy of bOR at Pt/mnV_(2)O_(6) was estimated to be 32 k J mol^(-1) and the order of reaction to be 0.51,indicating that borohydride hydrolysis proceeds in parallel with its ***-term stability of Pt/mnV_(2)O_(6) under bOR typical conditions was observed.A laboratory-scale direct borohydride fuel cell assembled with a Pt/mnV_(2)O_(6) anode reached a specific power of 274 W g^(-1).Experimental results on Pt/mnV_(2)O_(6) were complemented by DFT calculations,which indicated good adherence of Pt to mnV_(2)O_(6),beneficial for electrocatalyst stability.

关键词： borohydride oxidation reaction Platinum-based electrocatalyst manganese vanadate Density functional theory

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