Antiglycation,antioxidant,antiacne,and photoprotective activities of crude extracts and triterpene saponin fraction of Sapindus saponaria ***:An in vitro study

作     者：Regildo M.G.Silva Gustavo R.Martins Laura M.B.Nucci Filipe O.Granero Célia C.M.Figueiredo Patrícia S.Santiago Luciana P.Silva Regildo M.G.Silva;Gustavo R.Martins;Laura M.B.Nucci;Filipe O.Granero;Célia C.M.Figueiredo;Patrícia S.Santiago;Luciana P.Silva

作者机构：Laboratory of Herbal Medicine and Natural ProductsDepartment of BiotechnologyFaculty of SciencesHumanities and LanguagesSão Paulo State UniversityAssisSão PauloBrazil Institute of ChemistrySão Paulo State UniversityAraraquaraSão PauloBrazil São Paulo State UniversityCampus de RegistroRegistroSão PauloBrazil Fundação Educacional do Município de AssisAssisSão PauloBrazil 

出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)

年 卷 期：2022年第12卷第9期

页      面：391-399页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by the São Paulo Research Foundation Grant-FAPESP No.2013/10039-1 

主　　题：Photoprotective AGE formation Free radical Antioxidant Triterpene saponin Sapindus saponaria 

摘      要：Objective:To evaluate the photoprotective,antioxidant,antiglycation,and antiacne activities of crude extract(CESs)and triterpene saponin fraction(TSSs)of Sapindus ***:HPLC-MS purification was performed on a Symmetry TM C18 *** saponins were identified by a UV *** activity was evaluated by DPPH and O_(2)^(−)radicals scavenging,and FRAP and TBARS *** activity was assessed by relative electrophoretic mobility and inhibition of advanced glycation end products(AGEs)***,antiacne activity was determined by inhibition of Cutibacterium acnes,and photoprotective effect was evaluated by Mansur’s ***:Most of the triterpene saponins detected in the fraction by HPLC-MS analysis were hederagenin as the *** and TSSs presented varying antioxidant activity in DPPH(CESs:75.69%and TSSs:83.65%),FRAP(CESs:425.39μM TE/g DW and TSSs:649.36μM TE/g DW),TBARS(CESs:42.96%and TSSs:52.16%)and O_(2)^(−)radicals scavenging(CESs:61.33%and TSSs:86.69%)*** and TSSs also exhibited antiglycation activity comparable to bovine serum albumin treated with *** addition,CESs and TSSs showed inhibition of AGE formation(34.48%and 61.85%,respectively).Antiacne activity against Cutibacterium acnes was observed with a minimum inhibitory concentration equal to minimum bactericidal concentration(CESs:36.11µg/mL and TSSs:18.34µg/mL).In photoprotective assays,CESs and TSSs showed maximum absorbance of 1.42 to 0.20 and 2.80 to 1.30,respectively,in the wavelength range of 260 to 400 ***,CESs and TSSs showed sun protection factors of 8.89 and 14.89,***:Sapindus saponaria fruit extracts show strong antioxidant potential and antiglycation activity against bovine serum albumin glycation and AGE ***,they presented antibacterial activity against Cutibacterium acnes and photoprotective effect against UV-A and UV-B.