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Gelatin-biofermentative unsulfated glycosaminoglycans semi-interpenetrating hydrogels via microbial-transglutaminase crosslinking enhance osteogenic potential of dental pulp stem cells

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Regenerative Biomaterials 2021年第3期8卷 110-123页

作者： Annalisa La Gatta Virginia Tirino Marcella Cammarota Marcella La Noce Antonietta Stellavato Anna Virginia Adriana Pirozzi Marianna Portaccio Nadia Diano Luigi Laino gianpaolo papaccio Chiara Schiraldi Dipartimento di Medicina Sperimentale Universita`della Campania“Luigi Vanvitelli”via L.De Crecchio 7Naples 80138Italy Dipartimento Multidisciplinare di Specialita’Medico-Chirurgiche e Odontoiatriche via Luigi De Crecchio6Napoli 80138Italy

Gelatin hydrogels by microbial-transglutaminase crosslinking are being increasingly exploited for tissue engineering,and proved high potential in bone *** study aimed to evaluate,for the first time,the combination of enzymatically crosslinked gelatin with hyaluronan and the newly developed biotechnological chondroitin in enhancing osteogenic *** enzymatic crosslinking was carried out in the presence of hyaluronan or of a hyaluronan–chondroitin mixture,obtaining semi-interpenetrating *** latter proved lower swelling extent and improved stiffness compared to the gelatin matrix alone,whilst maintaining high *** heteropolysaccharides were retained for 30 days in the hydrogels,thus influencing cell response over this *** evaluate the effect of hydrogel composition on bone regeneration,materials were seeded with human dental pulp stem cells and osteogenic differentiation was *** expression of osteocalcin(OC)and osteopontin(OPN),both at gene and protein level,was evaluated at 7,15 and 30 days of *** electron microscopy(SEM)and two-photon microscope observations were performed to assess bone-like extracellular matrix(ECM)deposition and to observe the cell penetration *** the presence of the heteropolysaccharides,OC and OPN expression was upregulated and a higher degree of calcified matrix formation was *** with hyaluronan and chondroitin improved both the biophysical properties and the biological response of enzymatically crosslinked gelatin,fastening bone deposition.

关键词： hydrogels gelatin hyaluronan biotechnological chondroitin bone regeneration human dental pulp stem cells

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