Gelatin-biofermentative unsulfated glycosaminoglycans semi-interpenetrating hydrogels via microbial-transglutaminase crosslinking enhance osteogenic potential of dental pulp stem cells
作者机构：Dipartimento di Medicina SperimentaleUniversita`della Campania“Luigi Vanvitelli”via L.De Crecchio 7Naples 80138Italy Dipartimento Multidisciplinare di Specialita’Medico-Chirurgiche e Odontoiatrichevia Luigi De Crecchio6Napoli 80138Italy
出 版 物：《Regenerative Biomaterials》 (再生生物材料（英文版）)
年 卷 期：2021年第8卷第3期
页 面：110-123页
核心收录：
学科分类：081702[工学-化学工艺] 08[工学] 0817[工学-化学工程与技术]
基 金：This work was supported by Dipartimento di Medicina Sperimentale(Universita` della Campania“Luigi Vanvitelli”)-Progetti di ricerca scientifica di dipartimento anno 2018 Scientific research funding (2018)entitled“Novel biomaterials and stem cells for bone regener ation”
主 题：hydrogels gelatin hyaluronan biotechnological chondroitin bone regeneration human dental pulp stem cells
摘 要：Gelatin hydrogels by microbial-transglutaminase crosslinking are being increasingly exploited for tissue engineering,and proved high potential in bone *** study aimed to evaluate,for the first time,the combination of enzymatically crosslinked gelatin with hyaluronan and the newly developed biotechnological chondroitin in enhancing osteogenic *** enzymatic crosslinking was carried out in the presence of hyaluronan or of a hyaluronan–chondroitin mixture,obtaining semi-interpenetrating *** latter proved lower swelling extent and improved stiffness compared to the gelatin matrix alone,whilst maintaining high *** heteropolysaccharides were retained for 30 days in the hydrogels,thus influencing cell response over this *** evaluate the effect of hydrogel composition on bone regeneration,materials were seeded with human dental pulp stem cells and osteogenic differentiation was *** expression of osteocalcin(OC)and osteopontin(OPN),both at gene and protein level,was evaluated at 7,15 and 30 days of *** electron microscopy(SEM)and two-photon microscope observations were performed to assess bone-like extracellular matrix(ECM)deposition and to observe the cell penetration *** the presence of the heteropolysaccharides,OC and OPN expression was upregulated and a higher degree of calcified matrix formation was *** with hyaluronan and chondroitin improved both the biophysical properties and the biological response of enzymatically crosslinked gelatin,fastening bone deposition.
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