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Identification of the Elusive Chloroplast Ascorbate Transporter Extends the Substrate Specificity of the PHT Family

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Molecular Plant 2015年第5期8卷 674-676页

作者： Fernie, Alisdair R. toth, Szilvia Z. Max Planck Inst Mol Plant Physiol D-14476 Potsdam Germany Hungarian Acad Sci Inst Plant Biol Biol Res Ctr H-6701 Szeged Hungary

Most of the enzymatic reactions of ascorbate biosynthesis occur in the cytosol, with the exception of the terminal step, catalyzed by L-galactono-l,4-1actone dehydrogenase, which is localized on the inner mitochondrial membrane. Given the location of L-galactono-l,4-1actone dehydrogenase, the rate of ascorbate biosynthesis is intimately associated with the rate of mitochon- drial respiration （Nunes-Nesi et al., 2005）. Ascorbate has legion roles in the plant cell including mitosis, cell division, expansion and cell wall hydroxylation, hormone biosynthesis, signal transduction, programmed cell death, as well as protection against environmental stresses via detoxification of reactive oxygen species and regeneration of other antioxidants. This list has recently expanded with the epigenetic role exerted as a cofactor for DNA and histone demethylases in the nucleus and ascorbate may also be implicated in iron uptake （Pick and Weber, 2014）.

关键词： ElusiveChloroplast Extends Specificity

Diurnal variation of aethalometer correction factors and optical absorption assessment of nucleation events using multi-wavelength photoacoustic spectroscopy

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Journal of Environmental Sciences 2019年第9期31卷 96-109页

作者： Tibor Ajtai Gergely Kiss-Albert Noemi Utry Adam toth Andras Hoffer Gabor Szabo Zoltan Bozoki Department of Optics and Quantum Electronics University of Szeged H-6720 Szeged Hungary Hilase Development Production Service and Trading Limited Szekesfehervar H-8000 Hungary MTA-SZTE Research Group on Photoacoustic Spectroscopy H-6720 Szeged Hungary ELI-HU Non-Profit Ltd H-6720 Szeged Hungary Department of Earth and Environmental Sciences University of Pannonia P.O. Box 158 Veszprem H-8201 Hungary MTA-PE Air Chemistry Research Group P.O. Box 158 Veszprem H-8201 Hungary

A field measurement campaign was carried out during the late winter and early spring of2015 in Budapest, the capital of Hungary. The size distribution(SD) and optical absorption of carbonaceous particulate matter(CPM) was measured online using a Scanning Mobility Particle Sizer(SMPS), a 7λ-aethalometer and an inhouse developed 4λ-Photoacoustic Spectrometer. Based on the SD data, the measurement period could be classified into days with and without new particle formation events(normal days and nucleation days),although particular nucleation-like events were observed on normal days as well. Three characteristic size modes were observed with CMDs of circa 15, 25 and 110 nm that corresponded to the nucleation, traffic and heating modes. Based on the temporal behavior of these modes both types of days were divided into distinctive daily periods(heating hours,traffic hours and nucleation hours). The optical absorption spectra(OAC and AAE) also displayed the same part of day behavior to that of SD. That way this paper is among the first to assess the optical response of urban nucleation events. Due to the simultaneous measurement of OAC by the 7λ-aethalometer and a 4λ-Photoacoustic Spectrometer, OAC was measured overall at 11 wavelengths. That way aethalometer correction factors(f and C)were determined at all aethalometer wavelengths using in situ reference photoacoustic measurements. Correction factors were found to have both wavelength and time of the day variation. In the case of f, no clear trend could be observed, however, Cref values increased both as a function of wavelength.

关键词： Photoacoustic spectroscopy Aerosol Angstr?m exponent Aethalometer Nucleation Size distribution

Subsurface accumulation of CaCO3 and Cl- from groundwater under black locust and poplar plantations

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Journal of Forestry Research 2019年第4期30卷 1353-1361页

作者： Andras Szabo Zoltan Gribovszki Esteban Gabriel Jobbagy Kitti Balog Andras Bidlo Tibor toth Forest Research Institute National Agricultural Research and Innovation CentreVarkeriilet 30/ASarvar 9600Hungary Institute of Geomatics and Civil Engineering Faculty of ForestryUniversity of SopronBajcsy-Zsilinszky u.4Sopron 9400Hungary Grupo de Estudios Ambientales-IMASL Universidad Nacional de San Luis&CONICETEjercito de ios Andes 950D5700HHW San LuisArgentina Institute for Soil Sciences and Agricultural Chemistry Centre for Agricultural ResearchHASHerman O u.15Budapest 1022Hungary Institute of Environmental and Earth Sciences Faculty of ForestryUniversity of SopronBajcsy-Zsilinszky u.4Sopron 9400Hungary

When conditions are similar,more water evaporates from forest plantations than herbaceous vegetation,thereby affecting hydrological fluxes and ion transport in the *** vertical distribution of CaCO3 and Cl^-ions shifts due to *** effect of groundwater depth and clay content were studied in the Great Hungarian Plain where forest area has been increasing for decades by analyzing soil and groundwater samples from stands of black locust(Robinia pseudoacacia,11 plots)and poplar(Populus spp.,11 plots).All study sites contained one herbaceous(control)and one or more forested ***3 and Cl^-ions accumulated in the soil profile in greater quantities under tree cover than in the *** scale of this process largely depended on the species and on soil and ion *** black locust,Cl^-accumulated between 1.3 and 6.3 m,with a maximum difference of 0.3 pCl unit(pCl is Cl^-activity,the negative of the logarithm to base 10 of the concentration of the chloride ion,determined using an ion-selective electrode,it is a dimensionless quantity.),while the difference in CaCO3 accumulation was at most 3.5%in some layers,compared to control *** result may be explained by the difference in the mobility of Ca+and Cl^-*** mechanisms were noticeable under poplar plantations due to their higher water uptake:Cl-accumulation was detected below 0.9 m to the groundwater with a maximum difference of 0.5 pCl units,while CaCO3 accumulation was continuous at depths of 2.3–6.8 m with a maximum difference of 8.4%,compared to the *** increasing clay content,there was a discernible effect on CaCO3 and Cl-accumulation under black locust,but not observed under *** differences were explained by the differences in water uptake mechanisms and root patterns of the two species and the different mobility of Ca2^+and Cl-ions.

关键词： Tree plantations Ion accumulation Water regime Water uptake strategy Root structure

Insight into the antifungal mechanism of Neosartorya fischeri antifungal protein

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Protein & Cell 2015年第7期6卷 518-528页

作者： Mate Viragh Annamaria Marton Csaba Vizier Liliana toth Csaba Vagvolgyi Florentine Marx Laszlo Galgoczy Department of Microbiology Faculty of Science and Informatics University of Szeged Szeged Hungary Institute of Biochemistry Biological Research Centre Hungarian Academy of Sciences Szeged Hungary Division of Molecular Biology Biocenter Innsbruck Medical University Innsbruck Austria

Small, cysteine-rich, highly stable antifungal proteins secreted by filamentous Ascomycetes have great po- tential for the development of novel antifungal strate- gies. However, their practical application is still limited due to their not fully clarified mode of action. The aim of this work was to provide a deep insight into the anti-fungal mechanism of Neosartorya fischeri antifungal protein （NFAP）, a novel representative of this protein group. Within a short exposure time to NFAP, reduced cellular metabolism, apoptosis induction, changes in the actin distribution and chitin deposition at the hyphal tip were observed in NFAP-sensitive Aspergillus nidulans. NFAP did show neither a direct membrane disrupting- effect nor uptake by endocytosis. Investigation of A. nidulans signalling mutants revealed that NFAP acti- vates the cAMP/protein kinase A pathway via G-protein signalling which leads to apoptosis and inhibition of polar growth. In contrast, NFAP does not have any in- fluence on the cell wall integrity pathway, but an un- known cell wall integrity pathway-independent mitogen activated protein kinase A-activated target is assumed to be involved in the cell death induction. Taken to- gether, it was concluded that NFAP shows similarities, but also differences in its mode of antifungal action compared to two most investigated NFAP-related pro-teins from Aspergillus giganteus and Penicillium chrysogenum.

关键词： Neosartorya fischeri antifungal protein,Aspergillus nidulans cAMP/Pka signalling Pkc/Mpksignalling antifungal mechanism

X-ray-induced radiophotodynamic therapy （RPDT） using lanthanide micelles： Beyond depth limitations

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Nano Research 2015年第7期8卷 2373-2379页

作者： Slavka Kascakova Alexandre Giuliani Sara Lacerda Agnes Pallier Pascal Mercere eva toth Matthieu Refregiers Synchrotron SOLEIL L'Orme des Merisiers 91190 Gif-sur-Yvette France Universite Paris-Sud XI UMR-S I 193 94800 Villejuif France INRA UAR 1008 Caracterisation et elaboration des Produits Issus de I'Agriculture 44316 Nantes France Centre de Biophysique Moleculaire CNRS Rue Charles Sadron 45071 Orleans Cedex 2 France

We report lanthanide-based micelles integrating hypericin （Hyp） for X-ray- triggered photodynamic therapy （PDT）. The lanthanide luminescence induced by X-ray irradiation excites the photosensitizer, which leads to the generation of singlet oxygen. This versatile approach can be extended to other photosensitizers or other types of liponanoparticles and can allow for magnetic resonance imaging （MRI） guidance.

关键词： photodynamic therapy liponanoparticles,x-ray deep tumour photosensitizer

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建筑与自然的完美融合意大利芙拉总部

室内设计与装修 2022年第7期 92-96页

作者： [意大利]geza Architettura Fernando Guerra(摄影) 沙漏(编译) geza Architettura

经典皮革品牌芙拉(Furla)的新总部位于意大利塔瓦尔内莱瓦尔迪佩萨著名的葡萄酒产区基安蒂(Chianti)。该项目设计的核心是将工业建筑与意大利本土景观融为一体,激发人们进一步探寻优质工作空间的热情。芙拉总部园区占地42000 m^(2),建... 详细信息

经典皮革品牌芙拉(Furla)的新总部位于意大利塔瓦尔内莱瓦尔迪佩萨著名的葡萄酒产区基安蒂(Chianti)。该项目设计的核心是将工业建筑与意大利本土景观融为一体,激发人们进一步探寻优质工作空间的热情。芙拉总部园区占地42000 m^(2),建筑面积18300 m^(2),主要由办公区、实验室以及物流部3个部分组成。藏身于自然之中的建筑,与基安帝地区广袤的丘陵、工业遗产以及农业景观完美融合在一起.

关键词：农业景观办公区完美融合葡萄酒产区实验室意大利

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迪氏局部麻醉学

中华医学杂志 1935年第2期 155-180页

作者： geza DE TAKATS 杨铭鼎南京鼓楼医院外科系

目次牛序(***’s preface)莫序(McCracken’s forewold)译者自序堪序(Kanavel’s foreword)迪序(De Takats’s preface) 第一章概论一.局部麻醉学之简史 (一)压紧法(二)冷之应用法(三)电透法二.麻醉技术与制药学上进展之沿革 (一)麻醉...

目次牛序(***’s preface)莫序(McCracken’s forewold)译者自序堪序(Kanavel’s foreword)迪序(De Takats’s preface) 第一章概论一.局部麻醉学之简史 (一)压紧法(二)冷之应用法(三)电透法二.麻醉技术与制药学上进展之沿革 (一)麻醉技术之进展一览(二)制药学上之进展一览三.局部麻醉药

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卫生体系分类方法的创新

中国卫生政策研究 2017年第1期10卷 70-77页

作者： Federico toth 博洛尼亚大学政治和社会科学系意大利博洛尼亚40125

本文研究的是卫生体系分类问题,希望得到比以往分析的卫生组织模式更深入的成果。从卫生服务的筹资方式看,可在标准三分法(据此可将卫生体系分为三类:自愿保险、社会医保和全民医保)基础上增加两种类型:强制国民医保和特殊人群医保计划... 详细信息

本文研究的是卫生体系分类问题,希望得到比以往分析的卫生组织模式更深入的成果。从卫生服务的筹资方式看,可在标准三分法(据此可将卫生体系分为三类:自愿保险、社会医保和全民医保)基础上增加两种类型:强制国民医保和特殊人群医保计划。从保险方和服务提供方之间的服务提供和关系来看,有必要对垂直整合和独立的体系加以区分。本文与以往大部分研究的不同之处在于其潜在的逻辑。本文假设所有体系均为混合体系,主张抛开传统的卫生体系分类逻辑(即根据现行体系将各个国家分入不同类别),转而采用拼图逻辑。细分卫生服务或人口的概念,被证明对这一目标非常有用。

关键词：分类卫生体系 OECD 分类学医疗保险细分垂直整合

Electrical control of quantum emitters in a Van der Waals heterostructure

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Light(Science & Applications) 2022年第8期11卷 1790-1798页

作者： Simon J.U.White Tieshan Yang Nikolai Dontschuk Chi Li Zai-Quan Xu Mehran Kianinia Alastair Stacey Milos toth Igor Aharonovich School of Mathematical and Physical Sciences University of Technology SydneyUltimoNSW2007Australia ARC Centre of Excellence for Transformative Meta-Optical Systems University of Technology SydneyUltimoNSW2007Australia School of Physics University of MelbourneParkvilleVIC3010Australia School of Science RMIT UniversityMelbourneVIC3001Australia

Controlling and manipulating individual quantum systems in solids underpins the growing interest in the development of scalable quantum ***,hexagonal boron nitride(hBN)has garnered significant attention in quantum photonic applications due to its ability to host optically stable quantum ***,the large bandgap of hBN and the lack of efficient doping inhibits electrical triggering and limits opportunities to study the electrical control of ***,we show an approach to electrically modulate quantum emitters in an hBN-graphene van der Waals *** show that quantum emitters in hBN can be reversibly activated and modulated by applying a bias across the ***,a significant number of quantum emitters are intrinsically dark and become optically active at non-zero *** explain the results,we provide a heuristic electrostatic model of this unique ***,employing these devices we demonstrate a nearly-coherent source with linewidths of~160 *** results enhance the potential of hBN for tunable solid-state quantum emitters for the growing field of quantum information science.

关键词： quantum hexagonal boron