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The CUL3A–LFH1–UBC15 ubiquitin ligase complex mediates SHORT VEGETATIVE PHASE degradation to accelerate flowering at high ambient temperature

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Plant Communications 2024年第4期5卷 224-238页

作者： Suhyun Jin geummin youn Sun young Kim Taewook Kang Hyun-young Shin Ji-Yul Jung Pil Joon Seo Ji Hoon Ahn Department of Life Sciences Korea UniversitySeoul 02841Republic of Korea Department of Chemistry Seoul National UniversitySeoul 08826Republic of Korea These authors contributed equally to this article.

Ambient temperature affects flowering time in plants,and the MADS-box transcription factor SHORT VEGETATIVE PHASE(SVP)plays a crucial role in the response to changes in ambient *** protein stability is regulated by the 26S proteasome pathway and decreases at high ambient temperature,but the details of SVP degradation are ***,we show that SVP degradation at high ambient temperature is mediated by the CULLIN3–RING E3 ubiquitin ligase(CRL3)complex in Arabidopsis *** identified a previously uncharacterized protein that interacts with SVP at high ambient temperature and contains a BTB/POZ *** named this protein LATE FLOWERING AT HIGH TEMPERATURE 1(LFH1).Single mutants of LFH1 or CULLIN3A(CUL3A)showed late flowering specifically at 27***1 protein levels increased at high ambient *** found that LFH1 interacts with CUL3A in the cytoplasm and is important for SVP–CUL3A complex *** in CUL3A and/or LFH1 led to increased SVP protein stability at high ambient temperature,suggesting that the CUL3–LFH1 complex functions in SVP *** E2 ubiquitin-conjugating enzymes(UBCs)using RING-BOX PROTEIN 1(RBX1),a component of the CRL3 complex,as bait identified ***15 mutants also showed late flowering at high ambient *** vitro and in vivo ubiquitination assays using recombinant CUL3A,LFH1,RBX1,and UBC15 showed that SVP is highly ubiquitinated in an ATP-dependent ***,these results indicate that the degradation of SVP at high ambient temperature is mediated by a CRL3 complex comprising CUL3A,LFH1,and UBC15.

关键词： temperature-responsiveflowering SVP CUL3 proteasomal degradation ubiquitination

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FLOWERING LOCUS M isoforms differentially affect the subcellular localization and stability of SHORT VEGETATIVE PHASE to regulate temperature-responsive flowering in Arabidopsis

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Molecular Plant 2022年第11期15卷 1696-1709页

作者： Suhyun Jin Sun young Kim Hendry Susila Zeeshan Nasim geummin youn Ji Hoon Ahn Department of Life Sciences Korea UniversitySeoul 02841Republic of Korea

Temperature is an important environmental cue that affects flowering time in *** MADS-box transcription factor FLOWERING LOCUS M(FLM)forms a heterodimeric complex with SHORT VEGETATIVE PHASE(SVP)and controls ambient temperature-responsive flowering in ***-βand FLM-δ,two major splice variants produced from the FLM locus,exert opposite effects on flowering,but the molecular mechanism by which the interaction between FLM isoforms and SVP affects temperature-responsive flowering remains poorly ***,we show that FLM-βand FLM-δplay important roles in modulating the temperature-dependent behavior,conformation,and stability of *** localization of SVP decreases as temperature ***-βis required for SVP nuclear translocation at low temperature,whereas SVP interacts with FLM-δmainly in the cytoplasm at high *** preferentially binds to FLM-βat low temperature in tobacco leaf *** shows high binding affinity to FLM-βat low temperature and to FLM-δat high *** undergoes similar structural changes in the interactions with FLM-βand FLM-δ;however,FLM-δlikely causes more pronounced conformational changes in the SVP ***-δcauses rapid degradation of SVP at high temperature,compared with FLM-β,possibly via *** of lysine 53 or lysine 165 in SVP causes increased abundance of SVP due to reduced ubiquitination of SVP and thus delays flowering at high *** findings suggest that temperature-dependent differential interactions between SVP and FLM isoforms modulate the temperature-responsive induction of flowering in Arabidopsis.

关键词： flowering ambient temperature SVP FLM FLM-β FLM-δ nuclear translocation

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Chloroplasts prevent precocious flowering through a GOLDEN2-LIKE–B-BOX DOMAIN PROTEIN module

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Plant Communications 2023年第3期4卷 215-227页

作者： Hendry Susila Zeeshan Nasim Katarzyna Gawarecka Ji-Yul Jung Suhyun Jin geummin youn Ji Hoon Ahn Department of Life Sciences Korea UniversitySeoul 02841Republic of Korea

The timing of flowering is tightly controlled by signals that integrate environmental and endogenous *** produced by carbon fixation in the chloroplast are a crucial endogenous cue for floral *** also convey information directly to the nucleus through retrograde signaling to control plant growth and ***,we show that mutants defective in chlorophyll biosynthesis and chloroplast development flowered early,especially under long-day conditions,although low sugar accumulation was seen in some *** treated with the bleaching herbicide norflurazon also flowered early,suggesting that chloroplasts have a role in floral *** retrograde signaling mutants,the golden2-like 1(glk1)glk2 double mutants showed early flowering under long-day *** early flowering was completely suppressed by constans(co)and flowering locus t(ft)*** vascular-specific knockdown of both GLK1 and GLK2 phenocopied the glk1 glk2 ***1 and GLK2 repress flowering by directly activating the expression of B-BOX DOMAIN PROTEIN 14(BBX14),BBX15,and BBX16 via CCAATC cis-elements in the BBX ***14/15/16 physically interact with CO in the nucleus,and expression of BBXs hampered CO-mediated FT *** knockdown of BBX14/15/16 by artificial miRNA(35S::amiR-BBX14/15/16)caused early flowering with increased FT transcript levels,whereas BBX overexpression caused late *** of glk1/2 and 35S::amiR-BBX14/15/16 plants was insensitive to norflurazon *** these observations together,we propose that the GLK1/2-BBX14/15/16 module provides a novel mechanism explaining how the chloroplast represses flowering to balance plant growth and reproductive development.

关键词： Arabidopsis thaliana BBX chloroplast flowering GLK retrograde signaling

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