FLOWERING LOCUS M isoforms differentially affect the subcellular localization and stability of SHORT VEGETATIVE PHASE to regulate temperature-responsive flowering in Arabidopsis
作者机构：Department of Life SciencesKorea UniversitySeoul 02841Republic of Korea
出 版 物：《Molecular Plant》 (分子植物（英文版）)
年 卷 期：2022年第15卷第11期
页 面：1696-1709页
核心收录：
学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学]
基 金：National Research Foundation of Korea grant(NRF-2022R1A3B1078180to J.H.A.)
主 题：flowering ambient temperature SVP FLM FLM-β FLM-δ nuclear translocation
摘 要：Temperature is an important environmental cue that affects flowering time in *** MADS-box transcription factor FLOWERING LOCUS M(FLM)forms a heterodimeric complex with SHORT VEGETATIVE PHASE(SVP)and controls ambient temperature-responsive flowering in ***-βand FLM-δ,two major splice variants produced from the FLM locus,exert opposite effects on flowering,but the molecular mechanism by which the interaction between FLM isoforms and SVP affects temperature-responsive flowering remains poorly ***,we show that FLM-βand FLM-δplay important roles in modulating the temperature-dependent behavior,conformation,and stability of *** localization of SVP decreases as temperature ***-βis required for SVP nuclear translocation at low temperature,whereas SVP interacts with FLM-δmainly in the cytoplasm at high *** preferentially binds to FLM-βat low temperature in tobacco leaf *** shows high binding affinity to FLM-βat low temperature and to FLM-δat high *** undergoes similar structural changes in the interactions with FLM-βand FLM-δ;however,FLM-δlikely causes more pronounced conformational changes in the SVP ***-δcauses rapid degradation of SVP at high temperature,compared with FLM-β,possibly via *** of lysine 53 or lysine 165 in SVP causes increased abundance of SVP due to reduced ubiquitination of SVP and thus delays flowering at high *** findings suggest that temperature-dependent differential interactions between SVP and FLM isoforms modulate the temperature-responsive induction of flowering in Arabidopsis.
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