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Polyphenols Extracted from Enteromorpha clathrata Induce Apoptosis in Hepa1-6 Cell by Activating the Mitochondrial Apoptosis Signaling Pathways

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Journal of Ocean University of China 2023年第5期22卷 1393-1402页

作者： ZHANG Panxue SUN Bolun TONG Jingjing xia geran ZHANG Jinjie LI Chao YANG Wenge College of Food and Pharmaceutical Sciences Ningbo UniversityNingbo 315211China Key Laboratory of Animal Protein Food Deep Processing Technology of Zhejiang Province Ningbo UniversityNingbo 315211China

Green alga Enteromorpha clathrata(***)contains a variety of bioactive compounds,including polysaccharides,polyphenols and fat-soluble pigments etc.,among which polyphenols exhibit a wide range of medicinal *** polyphenols(ECPs)have shown various biological activities such as antioxidant,anti-inflammatory and antidiabetic effects;however,the potential of ECPs as an anti-cancer reagent remains *** aim of this study was to investigate the anti-tumor activity and underlying mechanisms of ECPs on hepatocellular *** cytotoxicity of Hepa1-6 cells was determined by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)and lactate dehydrogenase(LDH)*** cytometry and fluorescence microscope analysis of cell apoptosis after annexin V-fluorescein isothiocyanate(FITC)/propidium iodide(PI)staining.2',7'-dichlorodihydrofluorescein diacetate(DCFH-DA)assay was used for intracellular reactive oxygen species(ROS)***-9 activity was determined using cspase-9 colorimetric *** transmembrane potential(Δψm)was measured using *** blot and quantitative real-time PCR(qPCR)were used to assess the expressions of the apoptosis regulators Bax,Bcl-2,cytochrome c and *** was found that ECPs showed a dose-dependent cytotoxicity against Hepa1-6 cells by inducing *** apoptosis in ECPs-treated Hepa1-6 cells was accompanied by the loss of mitochondrial membrane potential,elevated ROS generation,increased release of mitochondrial cytochrome c,and up-regulation of caspase-9 and *** expressions of Bax(pro-apoptotic molecule)and Bcl-2(apoptosis suppressor)were up-regulated and down-regulated,respectively,at both mRNA and protein *** molecular alterations revealed that ECPs caused apoptosis of cells through the mitochondrial pathway,suggesting that ECPs are potential candidates to be developed for liver cancer treatment.

关键词： Enteromorpha clathrate polyphenol anti-cancer activity apoptosis mitochondrial-dependent pathway

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Influence mechanisms of different setting time at low temperature on the gel quality and protein structure of Solenocera crassicornis surimi

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Food Bioscience 2023年第1期51卷 1109-1116页

作者： Jingjing Tong Ru Jia geran xia Xinxin Zhang Shutong Zhang Huamao Wei Wenge Yang College of Food and Pharmaceutical Sciences Ningbo University315211China Key Laboratory of Animal Protein Food Deep Processing Technology of Zhejiang Province 315211China

The effect of setting time(0-36 h)at 4℃ on the quality changes of shrimp(Solenocera crassicornis)gel was evaluated in this *** quality of shrimp gel over setting time was evaluated by physical properties including gel strength,drip loss,moisture distribution and *** physical properties of the shrimp gel were effectively improved after more than 24 h of *** addition,the mechanism of the shrimp gel formation was also studied by combining chemical bonds,total sulfhydryl,disulfide bond,intrinsic fluorescence spectra and protein *** results indicated that the main forces affecting the physical properties of the shrimp gel were hydrogen bond and hydrophobic interaction,and the formation of disulfide bond during the setting process was beneficial to the construction of a uniform gel ***,setting for 24-30 h at 4℃ was an effectual way to improve the shrimp gel qualities.

关键词： Solenocera crassicornis Shrimp gel Setting time Protein structure Gel quality

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大果榉对不同低温的生理响应

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山西农业科学 2020年第10期48卷 1599-1601页

作者：关廉科卫格冉郭慧凤夏鎔杰张轶轩郭红彦山西农业大学林学院山西太谷030801

以大果榉叶片为试验材料,分别在-5、-10、-15、-20℃下进行低温处理,测定不同低温条件下大果榉叶片的丙二醛含量、可溶性蛋白含量、可溶性糖含量、超氧化物歧化酶活性,研究大果榉对不同低温的生理响应。结果表明,大果榉对于不同低温的... 详细信息

以大果榉叶片为试验材料,分别在-5、-10、-15、-20℃下进行低温处理,测定不同低温条件下大果榉叶片的丙二醛含量、可溶性蛋白含量、可溶性糖含量、超氧化物歧化酶活性,研究大果榉对不同低温的生理响应。结果表明,大果榉对于不同低温的生理响应存在差异,随着温度的降低,丙二醛含量逐渐增加,但在-15℃之后略有下降;可溶性蛋白和可溶性糖含量整体呈下降趋势,变化幅度和趋势受温度影响;超氧化物歧化酶活性整体呈先升高后下降的趋势,其活性在-15℃后大幅降低。-15℃可能是大果榉叶片的最低忍受温度,低于此温度后大果榉叶片将无法正常生长代谢。

关键词：大果榉低温胁迫生理指标抗寒性

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